Effect of Frequency of Administration of Kappa-Carrageenan on Immune Response and Survival of Vibrio parahaemolyticus Infected Vannamei Shrimp (Litopenaeus vannamei)
DOI:
10.29303/jbt.v25i3.9825Published:
2025-07-25Downloads
Abstract
Whiteleg shrimp (Litopenaeus vannamei) cultivation activities are often exposed to disease-causing pathogens, one of which is a bacterial disease from the genus Vibrio sp. One of the triggers is the accumulation of organic matter, leftover feed and feces in the waters which causes whiteleg shrimp to be toxic to NH3. Kappa carrageenan is a polysaccharide compound derived from red seaweed that has the potential as a natural immunostimulant. The study aimed to determine the effect of administering kappa carrageenan at different frequencies on the defense system of shrimp infected with Vibrio parahaemolyticus bacteria. The study used RAL, with 5 treatments and 3 replications. The highest Survival Rate (SR) value was found in P5 at 80% and the lowest in P3 at 22%. The highest RPS value was found in P5 at 94.82% which was given carrageenan once a week and the lowest in P3 at 82.18% which was given carrageenan every day. The highest THC amount was observed in P5 at 14.9 x 106 cells/ml and the lowest was in P1 at 5.01 x 106 cells/ml. The highest AF value was in the positive control treatment P1 66.6% and the lowest P2 negative control 44.2%. The highest TBC value was P1 1.24 CFU/mL and the lowest P5 0.29 CFU/mL. The highest TVC observation was P5 1.42 CFU/mL and the lowest was P1 0.18 CFU/mL. In conclusion, the administration of k-carrageenan 20 g/kg once every 7 days provided a better immune response and protection against shrimp infected with Vibrio parahaemolyticus bacteria.
Keywords:
Immunostimulant, Kappa carrageenan, Vaname shrimp, Vibriosis.References
Azhar, F., Mukhlis, A., Lestari, D. P., & Marzuki, M. (2023). Application of Kappa-carrageenan as Immunostimulant Agent in Non-Specific Defense System of Vannamei shrimp. AACL Bioflux, 16(1), 616-624.
Darwantin, K., Sidik, R., & Gunanti, M. (2016). Efisiensi Penggunaan Imunostimulan dalam Pakan terhadap Laju Pertumbuhan , Respon Imun dan Kelulushidupan Udang Vannamei (Litopenaeus vannamei). Jurnal Biosains Pascasarjana, 18(2), 3–17.
Direktorat Jenderal Perikanan Budidaya. (2024). Laporan kinerja 2024. Kementerian Kelautan dan Perikanan Republik Indonesia.
Febriani, D., Marlina, E., & Oktaviana, A. (2018). Total Hemosit Udang Vaname (Litopenaeus vannamei) yang Dipelihara pada Salinitas 10 ppt dengan Padat Tebar Berbeda. Journal of Aquaculture Science, 3(1), 276585.
Hapizah, I. A., Junaidi, M., & Azhar, F. (2024). Effectiveness of Use of UV Lamp in Disinfection of Additioned Vaname Shrimp (Litopenaeus vannamei) Cultivation Media Bacteria Vibrio Harveyi. Jurnal Biologi Tropis, 24(2), 954-965. https://doi.org/10.29303/jbt.v24i2.6889
Ir Harlina, M. P. (2023). Monograf Potensi Bahan Alami Dalam Peningkatan Sistem Imun Udang Vaname. Nas Media Pustaka, 53-54.
Li, H., Zhao, X., Wang, Y., & Liu, Q. (2021). Effect of Dietary Polysaccharides on Immune Function of Aquatic Animals: A Review. Aquaculture Nutrition, 27(3), 675–688. https://doi.org/10.1111/anu.13177
Muahiddah, N., Affandi, R., & Diamahesa, W. (2022). The Effect of Immunostimulants From Natural Ingredients on Vanamei shrimp (Litopenaeus vannamei) in Increasing Non-Specific Immunity to Fight Disease. Journal of Fish Health, 2(2), 90-96. https://doi.org/10.29303/jfh.v2i2.1462
Mudiarti, L., Setiyowati, D., Kursistiyanto, N., & Alimin, N. (2023). Pengaruh Penambahan Alginat dalam Pakan terhadap Performa Pertumbuhan dan Efisiensi Pemanfaatan Pakan Udang Vannamei (Litopenaeus vannamei). Jurnal Perikanan, 4(2), 56-63. http://dx.doi.org/10.33772/jma.v8i1.28413
Navianda, M. A. (2020). Profil Hemosit Udang Vaname (Litopenaeus vannamei) Untuk Menduga Kualitas Perairan Tambak Di Kabupaten Probolinggo Jawa Timur (Doctoral dissertation, Universitas Brawijaya).
Novriadi, R., Fadhilah, R., Wahyudi, A. E., & Trullas, C. (2021). Effects of Hydrolysable Tannins on the Growth Performance, Total Haemocyte Counts and Lysozyme Activity of Pacific White Leg Shrimp Litopenaeus vannamei. Aquaculture Reports, 21, 100796. D OI: 10.1016/j.aqrep.2021.100796
Nuraeni, W., & Dailami, M. (2023). Pertumbuhan Udang Vaname (Litopenaeus vannamei) pada tambak Dengan Padat Penebaran Dan Jenis Kolam yang Berbeda di Balai Produksi Induk Udang Unggul dan Kekerangan (BPIU2K) Karangasem, Bali.
Nurdin, F., Bustamin, B., Ridwan, R., & Seniati, S. (2021). Kajian Perubahan Sifat Fisika Kimia Bakteri Vibrio Harveyi di Berbagai Jenis Media Pada Suhu Penyimpanan -20°C. Agrokompleks, 21(2), 25–33. https://doi.org/10.51978/japp.v21i2.334
Nurlatifah, S., Darmayasa, I. B. G., Julyantoro, P. G. S., & Sudaryatma, P. E. (2022). Penghambatan Faktor Virulensi Vibrio parahaemolyticus Menggunakan Isolat Bakteri dari Saluran Pencernaan Ikan Kerapu. Acta VETERINARIA Indonesiana, 10(3), 228-238. https://doi.org/10.29244/avi.10.3.228-238
Nuryati, S., Wahjuningrum, D., Zulhelmi, A., & Kurniaji, A. (2024). Efektivitas Ekstrak Seduhan Batang Pohon Pisang Ambon dalam Mencegah Infeksi Aeromonas hydrophila Pada Larva Ikan Nila. Media Akuakultur, 19(2), 55–62. http://dx.doi.org/10.15578/ma.19.2.2024.55-62
Rahmawati, N., & Larasati, R. B. (2019). Produksi Garam Farmasi dari Air Laut dengan Metode Zero Discharge Desalination di Kabupaten Pamekasan. Doctoral dissertation. Institut Teknologi Sepuluh Nopember.
Rekha, P. N., Sarkar, S., Raja, R. N., Panigrahi, A., Jaimin, B., Rajamanickam, S., & Sivagnanam, S. (2020). Dosage Minimization of Chlorine to Improve Water Quality and Its Applicability For Shrimp Larval Rearing Operations In Hatchery. Dr. C. Balachandran, 49(4), 19-28.
Rusydi, R., Natasya, S., Ayuzar, E., Khalil, M., & Adhar, S. (2022). Pengaruh Ekstrak Daun Mimba (Azadirachta indica) dalam Mengobati Infeksi Bakteri Vibrio alginolyticus Pada Udang Vannamei (Litopenaeus vannamei). Jurnal Perikanan Unram, 2(12), 268–279. Doi: 10.29303/jp.v12i2.305
Sri, M. (2023). Pengaruh Coating Telur Asin Terhadap Umur Simpan. Skripsi. Universitas Lampung.
Wulandari, D. (2018). Respon Imun Non Spesifik Udang Vanamei (Litopenaeus Vannamei) yang Telah Diberi Perlakuan Ekstrak Polisakarida Rumput Laut Eucheuma cottonii Untuk Melawan Penyakit WSSV. Doctoral dissertation. Universitas Brawijaya.
Yanuhar, U., & Caesar, N. R. (2022). Imunologi Molekuler untuk Ikan. Universitas Brawijaya Press, pp:37
Zhang, Q., Li, S., Wang, H., & Liu, Y. (2021). The Role of Gut Microbiota in Immune Homeostasis and Digestive Health in Aquatic Animals. Aquaculture Research, 52(3), 1021–1035. https://doi.org/10.1111/are.14955
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