Formulation of Papaya Leaf (Carica papaya L.) Decoction Extract Spray as a Botanical Insecticide against House Flies (Musca domestica)
DOI:
10.29303/jbt.v26i3.12529Published:
2026-07-13Downloads
Abstract
The housefly (Musca domestica) is a mechanical vector for various infectious diseases, the control of which is still dominated by synthetic pesticides. This study aims to formulate and test the efficacy of a spray preparation of papaya (Carica papaya L.) leaf aqueous extract with variations in concentration (F1: 20%, F2: 40%, and F3: 60%) against housefly mortality. The experimental design used three replications for each treatment, utilizing a total of 120 houseflies, with 10 flies placed in each test container. A negative control group was included to ensure validity. Observations were conducted every 10 minutes over a 1-hour exposure period, and the collected data were analyzed descriptively. The results showed that an increase in extract concentration was directly proportional to the speed and rate of fly mortality. The negative control showed no mortality (0.00%). The F1 formulation resulted in an average mortality of 43.33%, while F2 and F3 demonstrated very high efficacy at 86.66% and 90.00%, respectively. Based on the killing speed, F3 recorded the highest cumulative mortality within the first 10 minutes (17 flies) compared to F2 (8 flies) and F1 (2 flies). The 40% (F2) and 60% (F3) concentration papaya leaf extract spray preparations possess potential and environmentally friendly insecticidal activity.
Keywords:
Aqueous extract Botanical insecticide Carica papaya leaves Housefly MortalityReferences
Abobakr, Y., Al-Hussein, F. I., Bayoumi, A. E., Alzabib, A. A., & Al-Sarar, A. S. (2022). Organophosphate Insecticides Resistance in Field Populations of House Flies, Musca domestica L.: Levels of Resistance and Acetylcholinesterase Activity. Insects, 13(2). DOI: https://doi.org/10.3390/insects13020192
Adnyani, N. L. T. W., Aisyah, R., & Puspaningrat, L. P. D. (2023). Formulasi dan uji efektivitas sediaan spray ekstrak bunga kecombrang (Etlingera elatior (Jack) R.M.Sm.) sebagai repellent terhadap nyamuk Aedes aegypti. Jurnal Farmasi Kryonaut, 2(2), 1–10. DOI: https://doi.org/10.59969/jfk
Barbehenn, R. V., & Peter Constabel, C. (2011). Tannins in plant-herbivore interactions. In Phytochemistry (Vol. 72, Number 13, pp. 1551–1565). DOI: https://doi.org/10.1016/j.phytochem.2011.01.040
Desai, S. D., Desai, D. G., & Kaur, H. (2009). Saponins and their biological activities. Pharma Times, 41(3), 13–16.
Rowe, R. C., Sheskey, P. J., & Quinn, M. E. (Eds.). (2009). Handbook of pharmaceutical excipients (6th ed.). London: Pharmaceutical Press.
Marjuni, M., Minarto, O., & Wahyono, S. C. (2021). Modifikasi Sirkulasi Air Pendingin Alat Destilasi pada Proses Pembuatan Akuades. Jurnal Fisika Flux: Jurnal Ilmiah Fisika FMIPA Universitas Lambung Mangkurat, 18(1), 16. DOI: https://doi.org/10.20527/flux.v18i1.8888
Pereira, V., Figueira, O., & Castilho, P. C. (2024). Flavonoids as Insecticides in Crop Protection—A Review of Current Research and Future Prospects. In Plants (Vol. 13, Number 6). Multidisciplinary Digital Publishing Institute (MDPI). DOI: https://doi.org/10.3390/plants13060776
Piri, M., Sumampouw, H. M., Moko, E. M., Kamagi, D. W., & Lawalata, H. (2022). Uji Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) Sebagai Insektisida Alami Lalat Rumah (Musca domestica). JURNAL BIOS LOGOS, 12(2), 114-121. DOI: https://doi.org/10.35799/jbl.v12i2.41034
Pokhrel, S., & Karki, P. (2021). Phytochemical Screening, Antioxidant and Antidiabetic Activities of Extracts of Leaves and Seeds of Carica papaya. Nepal Journal of Science and Technology, 20(1), 126–135. DOI: https://doi.org/10.3126/njst.v20i1.43362
Kasim, K. P., Zaenab, & Riskyah, N. (2024). Perbandingan ekstrak daun pepaya (Carica papaya) dan daun srikaya (Annona squamosa) dalam mematikan lalat rumah (Musca domestica). Jurnal Sulolipu: Media Komunikasi Sivitas Akademika dan Masyarakat, 24(1), 58-67. DOI: https://doi.org/10.32382/sulo.v24i1.472
Purnomo, Y., & Tilaqza, A. (2022). Aktivitas Analgesik Infusa Dan Dekokta Daun Pulutan (Urena lobata). Jurnal Wiyata: Penelitian Sains Dan Kesehatan, 9(1), 8. DOI: https://doi.org/10.56710/wiyata.v9i1.586
Salim, R., Taslim, T., Selonni, F., Verawaty, V., & Dewi, I. P. (2025). Decoction of Cocos nucifera Linn. : Antioxidant Activity, Total Phenolics, and Flavonoids Contents. Indonesian Journal of Pharmaceutical Science and Technology, 12(2), 129–136. DOI: https://doi.org/10.24198/ijpst.v12i2.45058
Souto, A. L., Sylvestre, M., Tölke, E. D., Tavares, J. F., Barbosa-Filho, J. M., & Cebrián-Torrejón, G. (2021). Plant-derived pesticides as an alternative to pest management and sustainable agricultural production: Prospects, applications and challenges. In Molecules (Vol. 26, Number 16). MDPI AG. DOI: https://doi.org/10.3390/molecules26164835
Utami, D. T., Nofita, & Ulfa, A. M. (2022). Formulasi dan evaluasi fisik sediaan spray ekstrak daun kemangi (Ocimum x africanum Lour.) sebagai repellent alami terhadap nyamuk Aedes aegypti. Acta Pharmaceutica Indonesia, 47(2), 9–15. DOI: https://doi.org/10.5614/api.v47i2.19480
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