Aktivitas Antibakteri Ekstrak Metanol Daun Turi (Sesbania grandiflora L.) Terhadap Pertumbuhan Klebsiella pneumoniae
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Huurun Iien , Lalu Zulkifli , Prapti SedijaniDOI:
10.29303/jbt.v20i2.1790Published:
2020-06-06Issue:
Vol. 20 No. 2 (2020): Mei - AgustusKeywords:
Turi Leaf Extract, maceration, antimicrobial, Klebseilla pneumoniae, Secondary MetabolitesArticles
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Abstract
Abstrak: Sesbania grandiflora (tanaman turi) memiliki berbagai khasiat dalam pengobatan penyakit termasuk yang dapat digunakan sebagai campuran dalam persiapan bahan antibakteri. Tumbuhan ini mengandung senyawa metabolit sekunder yang dapat memiliki aktivitas sebagai zat antibakateri. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder ekstrak methanol daun turi dan mengetahui bioaktivitas ekstrak tersebut terhadap pertumbuhan bakteri Klebsiella pneumoniae. Daun S. grandiflora diekstraksi dengan metode maserasi menggunakan pelarut metanol. Hasil uji fitokimia secara kualitatif menunjukkan bahwa ekstrak metanol daun turi mengandung senyawa metabolit sekunder seperti tannin, saponin, dan terpenoid. Uji penghambatan sifat antibakteri dilakukan berdasarkan metode difusi sumuran, dengan mengukur rata-rata zona hambat yang terbentuk pada media MHA. Hasil penelitian menunjukkan bahwa ekstrak metanol dari daun S. grandiflora dengan konsentrasi 10%, 25%, 40%, dan 55% menunjukkan diameter rata-rata zona hambatan masing-masing 7,2, 14,4, 17,9, dan 22,5 mm. Kemampuan untuk menghambat pertumbuhan bakteri pada konsentrasi 55%, 40%, dan 25% dikategorikan sebagai penghambatan kuat karena memiliki diameter zona hambat rata-rata lebih dari 12 mm (sesuai dengan kategori Vasanthakumari). Konsentrasi yang semakin tinggi memberikan efek penghambatan yang lebih kuat. Hasil di atas menunjukkan bahwa kandungan ekstrak daun tanaman turi dapat digunakan sebagai sumber bahan obat baru di masa depan melalui penelitian lebih lanjut.
Kata kunci: Ekstrak Daun Turi, maserasi, antibakteri, Klebsiella pneumoniae, metabolit sekunder
Abstract: Sesbania grandifloraÃÂ (Turi plant) has various medicinal properties, including as a mixture in the preparation of antibacterial ingredients. This plant contains secondary metabolites that can have antibacterial activity. This study aims to determine the secondary metabolite content of the turi leaf methanol extract and determine the bioactivity of the extract on the growth ofÃÂ Klebsiella pneumoniae.ÃÂ S. grandifloraÃÂ leaves were extracted using the maceration method using methanol as a solvent. Phytochemical test results show that the turi leaf methanol extract contains secondary metabolites such as tannin, saponin, and terpenoids. The antibacterial inhibition test was carried out based on the well diffusion method, by measuring the average of the zone of inhibition formed on the MHA media. The results showed that the methanol extract of the leaves ofÃÂ S. grandifloraÃÂ with concentrations of 10%, 25%, 40%, and 55% showed an average diameter of inhibition zones 7.2, 14.4, 17.9, and 22, 5 mm, respectively. The ability to inhibit bacterial growth at concentrations of 55%, 40%, and 25% were categorized as having a strong inhibitory power because it has an average inhibition zone diameter of more than 12 mm (according to the Vasanthakumari category). The higher concentration gives a stronger inhibitory effect. The above results show that the leaf of the turi plant can be used as a source of new medicinal ingredients in the future.ÃÂ ÃÂ
Keywords: Turi Leaf Extract, maceration, antimicrobial, Klebsiella pneumoniae, secondary metabolites
References
Ajizah, A., Thihana & Mirhanuddin (2007). Potensi Ekstrak Kayu Ulin (Eusideroxylon zwageri TetB) dalam Menghambat Pertumbuhan Bakteri Staphylococcus aureus Secara In Vitro. Bioscientiae. 4 (1): 37-42. DOI: (https://ppjp.ulm.ac.id/journals/index.php/bioscientiae/article/view/161).
Amaranti, W., Inur, T. & Aldi B. R. (2017). Uji Kandungan Saponin pada Daun, Tangkai Daun dan Biji Tanaman Turi (Sesbania grandiflora). Politeknik Tegal: Seminar Nasional 2nd IPTEK Terapan (SENIT). DOI: (http://ejournal.poltektegal.ac.id/index.php/SENIT2017/article/view/565).
Anderson, K.F., Lonsway, D.R. & Rasheed, J.K. (2007). Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae. J Clin Microbiol, 45, pp.2723-5. DOI: (https://www.ncbi.nlm.nih.gov/pubmed/17581941).
Arun, A., Karthikeyan, P., Sagadevan, P. A., Umamaheswari, R.A. & Rex-Peo, R. (2014). Phytochemical Screening of Sesbania grandifora Linn. Int.J. Biosci. Nanosci. 1 (2): 33-36. DOI: (https://www.semanticscholar.org/paper/Phytochemical-screening-of-Sesbania-grandiflora-Arun/2f8f788590f173cce00b81a6d7819c78662ad6ae#paper-header).
Arunabha, M. & Satish, N. (2015). Study the Immunomodulatory Effects of Combined Extracts of Sesbania grandiflora Flowers and Cocculus hirsutus Leaves on The Circulating Antibody Response. Am. J. Phytomed. Clin. Ther. 3 (3): 199-208. DOI: (https://www.imedpub.com/abstract/study-the-immunomodulatory-effects-ofrncombined-extracts-of-sesbania-grandiflorarnflowers-and-cocculus-hirsutus-leaves-onrnthe-circulating-antibody-response-10360.html).
Avalaskar A.N., Itankar P.R., Joshi V.S., Agrawal M. & Vyas J. (2011). Phytochemical and TLC Studies of Ethanolic Extract of Sesbania grandiflora (Fabaceae). Int.J. Pharm.Tech. Res. 3 (3): 1346-1349. DOI: (http://www.sphinxsai.com/Vol.3No.3/pharm/pdf/).
Bangun, A. (2012). Ensiklopedia Tanaman Obat Indonesia. Bandung: IPH
Beesley, T., Gascoyne, N. & KnottHunziker, V. (1983). The inhibition of class C ò-lactamases by boronic acids. Biochem J, 209, pp.229-33. DOI: (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1154076/).
Cavalieri, S.J., Rankin, I.D., Harbeck, R.J., Sautter, R.S., McCarter, Y.S., Sharp, S.E., Ortez., J.H. & Spiegel, C.A. (2005). Manual of Antimicrobial Susceptibility Testing. USA: American Society for Microbiology.
Cowan, M.M. (1999). Plant Products as Antimicrobial Agents. Clinical Microbiology Review. 12(4): 564-582. DOI: (https://www.ncbi.nlm.nih.gov/pubmed/10515903).
Dwidjoseputro, D. (2003). Dasar-Dasar Mikrobiologi. Djambatan. Jakarta.
Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Edisi ke-3. Chapman and Hall, New York. Halaman 4-6, 30-31, 60-61, 90-92, 108-110, 132-133, dan 203-205. DOI: https://www.springer.com/gp/book/9780412572609
Hariana, A.H. (2006). Tumbuhan Obat dan Khasiatnya seri 3. Jakarta: Penebar Swadaya.
Jawetz, M. & Adelberg, E. (2005). Mikrobiologi Kedokteran. Jakarta: Buku Kedokteran ECG.
Jones, W.P. & Kinghorn, A.D. (2006). Extraction of Plant Secondary Metabolites. In: Sharker, S.D. Latif Z., Gray A.L, eds. Natural Product Isolation. 2 nd edition. New Jersey: Humana Press.
Lestari, P. (2016). Studi Tanaman Khas Sumatera Utara yang Berkhasiat sebagai Obat. Akademi Farmasi Yayasan Tenaga Pembangunan Arjuna, Sumatera Utara.
Masduki (1996). Efek Antibakteri Ekstrak Biji Pinang (Areca catechu) terhadap S. aureus dan E. coli. Cermin Dunia Kedokteran, Jakarta.
Morin R.B. & M. Gorman (1995). Kimia dan Biologi Antibiotik ò-Lactam (Chemistry and Biology of b-Lactam Antibiotics). Edisi III. Diterjemahkan oleh Mulyani S. Semarang: IKIP Semarang Press.
Nikham & Taty E.B. (2012). Uji Baku Antibakteri dari Buah Mahkota Dewa (Phaleria Macrocarpa (Scheff) Boerl) Hasil Iradiasi Gamma dan Antibiotik Terhadap Bakteri Patogen. Dalam: Prosiding Pertemuan Ilmiah Ilmu.
Nuria, M. C., Faizatun, A. & Sumantri (2009). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha Curcas L) Terhadap Bakteri Staphylococcus Aureus ATCC 25923, Escherichia Coli ATCC, dan Salmonella Typhi ATCC 1408. Mediagro. 5(2):26ââ¬â37. DOI: (https://media.neliti.com/media/publications/149782-ID-none.pdf).
Padmalochana, K. & Rajan, D. M. S. (2014). Antimicrobial Activity of Aqueous, Ethanol and Acetone Extracts of Sesbania grandifora Leaves and Its Phytochemical Characterization. Int. J. of Pharma Sciences and Research. 5(12): 957-962. DOI: (http://www.ijpsr.info/docs/IJPSR14-05-12-035.pdf).
Panda, C., U.S. Mishra, S. Mahapatra, G. & Panigrahi (2013). Free Radical Scavenging Activity and Phenolic Content Estimation of Glinus oppositifolius and Sesbania grandiflora. Int. J. Pharm. 3(4): 722-727. DOI: (http://pharmascholars.com/abstractview/free-radical-scavenging-activity-and-phenolic-content-estimation-of-glinus-oppositifolius-and-sesbania-grandiflora).
Pratiwi, S.T. (2008). Mikrobiologi Farmasi. Jakarta: Erlangga.
Rachmawati, F., Nuria M. C. & Sumantri (2011). Uji Aktivitas Antibakteri Fraksi Kloroform Ektrak Etanol Pegagan (Centella asiantica (L) Urb) serta Identifikasi Senyawa Aktifnya. Semarang: Universitas Wahid. DOI: (https://scholar.google.co.id/citations?user=n8ksTikAAAAJ&hl=id).
Reji, A.F. & Alphonse, R.N. (2013). Phytochemical Study on Sesbania grandiflora. J. Chem. Pharm. Res. 5(2): 196-201. DOI: (https://www.researchgate.net/publication/281718054_Phytochemical_study_on_Sesbania_grandiflora).
Robinson, T. (1995). Kandungan Organik Tumbuhan Tingkat Tinggi. ITB: Bandung.
Sarathbabu, R.V.T. Ramani, B.K. Rao & S. Panda. (2012). Antibiotic Susceptibility Pattern of Klebsiella pneumoniae Isolated from Sputum, Urine and Pus Samples. IOSRJPBS. 1(2): 04-09. DOI: (http://iosrjournals.org/iosr-jpbs/papers/vol1-issue2/I0120409.pdf).
Sjoekoer, D.M., Roetiningsih & Sinarto, S. (2003). Bakteriologi Medik. Jakarta: Banyumedika.
Vandepitte, S. (2005). Prosedur Laboratorium Dasar untuk Bakteriologis Klinis. Edisi 2. Buku Kedokteran EGC, Jakarta. Vandepitte, S. 2005. Prosedur Laboratorium Dasar untuk Bakteriologis Klinis. Edisi 2. Buku Kedokteran EGC, Jakarta.
Vasanthakumari, R. (2009). Practical Microbiology. New Delhi: BI Publications Pvt Ltd.
Vinothini, K., Devi, M.S., Shalini, V., Sekar, S., Semwal, R.B. & Arjun, P. (2017). In Vitro Micropropagation, Total Phenolic Content and Comparative Antioxidant Activity of Different Extracts of Sesbania grandiflora (L.) Pers. Curr. Sci. 113 (6): 1142-1147. DOI: (https://www.researchgate.net/publication/320057976_In_vitro_micropropagation_total_phenolic_content_and_comparative_antioxidant_activity_of_different_extracts_of_Sesbania_grandiflora_L_Pers).
Author Biographies
Lalu Zulkifli, Program Studi Magister Pendidikan IPA Universitas Mataram, Mataram
Prapti Sedijani, Program Studi Magister Pendidikan IPA Universitas Mataram, Mataram
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