Larvacidal Effect of Methanol Extract from Soursop Leaves Against 3rd Instar Larvae of Culex sp. Mortality
Authors
Ni Putu Maitri Vidya Cita Riesky , Syamsul Bahri , Lalu JapaDOI:
10.29303/jbt.v22i4.3906Published:
2022-12-09Issue:
Vol. 22 No. 4 (2022): October - DecemberArticles
Downloads
How to Cite
Downloads
Metrics
Abstract
The Culex genus is widely distributed in Indonesia. It is responsible for disrupting and interfering of human daily activities and also transmitting some diseases for human. Vector control strategies normally involve the use of synthetic larvacide however excessive use causes adverse effect on environmental quality and resistance to larvae. Thus, extract from soursop (Annona muricata L.) leaves may be an alternative sources of larval control agents. The present study reveals the larvicidal effect of methanol extract from soursop leaves against 3rd instar larvae of Culex sp. mortality. Experimental method with Completely Randomized Design (CRD) consisting of 6 treatments (0 ppm, 125 ppm, 250 ppm, 500 ppm, 1000 ppm and 2000 ppm) and 4 replications was carried out with Culex sp. 3rd instar larvae and the larval mortality was recorded after 48 hours since the exposure. Data were analyzed using one way ANOVA which was continued by Tukey HSD and followed by Probit analyze to determine LC50 and LC90. The results showed that the methanol extract of soursop leaves exhibited significant larvicidal activity with LC50 and LC90 values are 586,099 and 2119,645 ppm. The highest concentration increases the mortality rate of larvae. In conclusion, the soursop leaves methanol extract has a larvacide effect on the 3rd larvae of Culex sp.
References
Ahdiyah, I., & Purwani, K. I. (2015). Pengaruh Ekstrak Daun Mangkokan (Nothopanax scutellarium) sebagai Larvasida Nyamuk Culex sp. Jurnal Sains dan Seni ITS, 4(2):32-36.
Ahmad, A., & Adriyanto. (2019). Efektivitas Serbuk Biji Pepaya (Carica Papaya L.) Terhadap Kematian Jentik (Larva) Culex sp. Medikes Media Informasi Kesehatan, 6(1), 104-112.
Amirullah, Malik, N., & Rosmaya. (2019). Efektivitas Ekstrak Daun Sirih (Piper betleL) dan Ekstrak Bawang Putih (Allium sativum) terhadap Mortalitas Larva Nyamuk Aedes aegypti. Jurnal Bionature, 20(1), 47-56.
Bagaskara, R. (2018). Pemetaan Sebaran Nyamuk Culex di Kecamatan Sandubaya Kota Mataram Menggunakan Geographic Information SYSTEM (GIS) sebagai Dasar Pengendalian Penyakit Filariasis. Kota Mataram: UIN Mataram.
Fantin, M., Garelli, F., Napoli, B., Forgiarini, A., Gumeni, S., Martin, D. S., Orso, G. (2019). Flavonoids Regulate Lipid Droplets Biogenesis in Drosophila melanogaster. Natural Product Communications, 14(5), 1-8.
Hidayati, L., & Suprihatini, S. (2020). Pengaruh Pemberian Ekstrak Biji Mahoni (Swietenia mahagoni) Terhadap Kematian Larva Culex sp. Aspirator, 12(1), 45-52.
Kardinan, A. (2002). Pestisida Nabati Ramuan dan Aplikasi. Jakarta: Swadaya.
Kartikasari, D., & Novitasari, M. (2018). Uji Aktivitas Larvasida Perasan Herba Seledri (Apium graveolens L.) Terhadap Larva Aedes Aegypti. As-Syifaa , 10 (2) : 152-160.
Kewa, M. M., Almet, J., & Laut, M. M. (2020). Median Lethal Concentration (LC50) Ekstrak Daun Sirsak (Annona Muricata Linninn) terhadap Larva Culex sp. Jurnal Kajian Veteriner, 8 (2) :147-152.
Khaer, A., & Ekawardana. (2017). Efektivitas Ekstrak Daun Sirsak (Annona muricata) dalam Membunuh Jentik Aedes aegypti. Jurnal Sulolipu : Media Komunikasi Sivitas Akademika dan Masyarakat , 17 (1) : 9-14.
Kuncoro, H. (2013). Aktivitas Larvasida Ekstrak Daun Tumbuhan Mara Tunggal (Clausena Excavata Burm.F) Dan Daun Zodia (Euodia Ridleyi Hochr) Terhadap Larva Nyamuk Culex Sp. J. Trop. Pharm. Chem, 2(2), 91-99.
Kurnia, B., & Astuti, F. (2019). Uji Efek Larvasida Ekstrak Daun Kelor (Moringa oleifera) terhadap Larva Vektor Demam Berdarah Dengue Aedes Aegypti. Bachelor thesis, Universitas Ahmad Dahlan
Neupane, P., Bhuju, S., Thapa, N., & Bhattarai, H. K. (2018). ATP Synthase: Structure, Function and Inhibition. BioMol Concepts 2, 10(1), 1-10.
Oktiansyah, R., Wicaksono, A., Armanda, , F., Nurokhman, A., Habisukan, U. H., Aini, K., & Hapida, Y. (2020). Biolarvacide of Culex quinquefasciatus. Biota: Biologi dan Pendidikan Biologi, 13(1) :1-11.
Pramadya, P., & Hendrayana , M. (2020). Efek Ekstrak Metanol Daun Sirsak (Annona Muricata) Dalam Menghambat Pertumbuhan BakterI Salmonella Typhi Secara In Vitro. Jurnal Medika Udayana, 9 (9) : 65-69.
Rahmah, L. A., Tresnani, G., & Suryadi, B. F. (2019). Identifikasi Jenis Nyamuk dan Karakteristik Habitatnya di Desa Kekeri Kecamatan Gunung Sari Kabupaten Lombok Barat. Biowallacea Jurnal Ilmiah Biologi, 5(1):36-42.
Rattan, R. S. (2010). Mechanism of action of insecticidal secondary metabolites of plant origin . Crop Protection, 29 (9) : 913-920.
Roopashree, K., & Naik, D. (2019). Saponins : Properties, Applications and as Insecticides: A Review. Trends in Bioscience, 12 (1) : 1-14.
Semwal, D. K. (2016). Myricetin: A dietary molecule with diverse biological activities. Nutrients, 8(2), 1–31
Sumihe, G., Runtuwene, M. R., & Rorong, J. A. (2014). Analisis Fitokimia dan Penentuan Nilai LC50 Ekstrak Metanol Daun Liwas. Jurnal Ilmiah Sains, 14(2), 125-128.
Supriadin, A. K. (2017). Efek Larvasida Hasil Fraksinasi Metanol Daun Aglaia glabrata terhadap Larva Aedes aegypti. JURNAL ISTEK, 10 (1) : 69-82.
Susanti, M., Kuncoro, H., & Rijai , L. (2015). Uji Aktivitas Larvasida Ekstrak Daun Keladi Birah (Alocasia indica Schott) terhadap Larva Nyamuk Culex sp. Jurnal Sains dan Kesehatan, 1 (1) : 5-10.
Wahyuni, D., Prajnaparamita, D., & Suratno. (2014). Toksisitas Granula Ekstrak Biji Alpukat (Persea Americana Mill.) Terhadap Mortalitas Larva Nyamuk Aedes aegypti L. Artikel Ilmiah Hasil Penelitian Mahasiwa Tahun, 1-5.
Wuillda de Seuza, A. C. J., Campos Martins, R. C., & Costa, F. D. N. (2019). Larvicidal activity of secondary plant metabolites in aedes aegypti control: An overview of the previous 6 years. Natural Product Communications, 14(7), 1934578X19862893.
License
Copyright (c) 2022 Ni Putu Maitri Vidya Cita Riesky, Syamsul Bahri, Lalu Japa
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.