Antibacterial Activity of Bitter Drugs from Lingga Malay Ethnic in Riau Archipelago Against Bacterial Causing Skin and Diarrhea Diseases
Authors
Rahma Dhani , Rodesia Mustika Roza , FitmawatiDOI:
10.29303/jbt.v23i1.4430Published:
2023-01-17Issue:
Vol. 23 No. 1 (2023): January - MarchArticles
Downloads
How to Cite
Downloads
Metrics
Abstract
Infection is the major threat to humanity caused by infectious agents. The most common infectious agent of human is the cause of skin and diarrhea diseases. Melayu Lingga people uses alternative medicine called obat pahit potion to treat skin and diarrhea diseases and mixes by Praktisi Obat Tradisional (POT). The purpose of this research was to determine antibacterial activity of the methanol extract from obat pahit potion against bacteria that cause skin diseases (Staphylococcus aureus ATCC 12600, Staphylococcus epidermidis ATCC 12228, Pseudomonas aeruginosa ATCC 10145) and diarrhea (Escherichia coli ATCC 11775, Salmonella typhi ATCC 14028, Shigella flexneri ATCC 12022) by using disc diffusion method with variation of extract concentration (0.75 gr/ml, 0.5 gr/ml, and 0.25 gr/ml). Antibacterial activity was determined by measuring the inhibitory zone formed around the disc. Based on the result of antibacterial activity test, the largest inhibitory zone was found in S.aureus ATCC 12600, S.epidermidis ATCC 12228 and P.aeruginosa ATCC 10145 shown in POT Musai at 0.25 mg/ml concentrations with value 6,61 mm; 6,10 mm; and 10,11 mm. Meanwhile, antibacterial activity against E.coli ATCC 11775 shown in TMP Musai at 0.5 gr/ml concentration with value 4,47 mm. Antibacterial activity against S.typhi ATCC 14028 and S.flexneri ATCC 12022 shown in TMP Musai at 0.25 gr/ml concentration with value 8,20 mm and 11,73 mm.
References
Akinduti, P. A., Emoh-Robinson, V., Obamoh-Triumphant, H. F., Obafemi, Y. D., & Banjo, T. T. (2022). Antibacterial activities of plant leaf extracts against multi-antibiotic resistant Staphylococcus aureus associated with skin and soft tissue infections. BMC complementary medicine and therapies, 22(1), 1-11. DOI: 10.1186/S12906-022-03527-Y.
Akinyemi, K. O., Oladapo, O., Okwara, C. E., Ibe, C. C., & Fasure, K. A. (2005). Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC complementary and alternative medicine, 5(1), 1-7. DOI: 10.1186/1472-6882-5-6.
Alzoreky, N. S. and Nakahara, K. (2003) ‘Antibacterial activity of extracts from some edible plants commonly consumed in Asia’, International Journal of Food Microbiology, 80(3), pp. 223–230. DOI: 10.1016/S0168-1605(02)00169-1.
Bessa, L. J., Fazii, P., Di Giulio, M., & Cellini, L. (2015). Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. International wound journal, 12(1), 47-52. DOI: 10.1111/IWJ.12049.
Castro, S. B. R., Leal, C. A. G., Freire, F. R., Carvalho, D. A., Oliveira, D. F., & Figueiredo, H. C. P. (2008). Antibacterial activity of plant extracts from Brazil against fish pathogenic bacteria. Brazilian Journal of Microbiology, 39, 756-760. DOI: 10.1590/S1517-838220080004000030.
DeLeon, S., Clinton, A., Fowler, H., Everett, J., Horswill, A. R., & Rumbaugh, K. P. (2014). Synergistic interactions of Pseudomonas aeruginosa and Staphylococcus aureus in an in vitro wound model. Infection and immunity, 82(11), 4718-4728. DOI: 10.1128/IAI.02198-14.
Elisha, I. L., Botha, F. S., McGaw, L. J., & Eloff, J. N. (2017). The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC complementary and alternative medicine, 17(1), 1-10. DOI: 10.1186/S12906-017-1645-Z/TABLES/3.
Enan, G., Al-Mohammadi, A. R., Mahgoub, S., Abdel-Shafi, S., Askar, E., Ghaly, M. F., ... & El-Gazzar, N. (2020). Inhibition of Staphylococcus aureus LC 554891 by Moringa oleifera seed extract either singly or in combination with antibiotics. Molecules, 25(19), 4583. DOI: 10.3390/MOLECULES25194583.
Farha, A. K., Yang, Q. Q., Kim, G., Li, H. B., Zhu, F., Liu, H. Y., ... & Corke, H. (2020). Tannins as an alternative to antibiotics. Food Bioscience, 38, 100751. DOI: 10.1016/J.FBIO.2020.100751.
Fazli, M., Bjarnsholt, T., Kirketerp-Møller, K., Jørgensen, B., Andersen, A. S., Krogfelt, K. A., ... & Tolker-Nielsen, T. (2009). Nonrandom distribution of Pseudomonas aeruginosa and Staphylococcus aureus in chronic wounds. Journal of clinical microbiology, 47(12), 4084-4089. DOI: 10.1128/JCM.01395-09.
Fitmawati, F., Sofiyanti, N., Roza, R. M., Isnaini, I., Hazimi, H., Irawan, Y. R., & Dewi, A. P. K. (2017). Traditional medicinal formulation: Obat pahit from lingga Malay ethnic in riau archipelago, Indonesia. Biodiversitas Journal of Biological Diversity, 18(3), 1196-1200. DOI: 10.13057/BIODIV/D180343.
Fitmawati, F., Sofiyanti, N., Roza, R. M., Isnaini, I., Irawan, Y. R., Winata, D. R., & Dewi, A. P. K. (2017). Antioxidant activity of dominant plants species in Obat pahit from Lingga Malay ethnic in Riau Archipelago. Biosaintifika: Journal of Biology & Biology Education, 9(2), 325-331. DOI: 10.15294/biosaintifika.v9i2.9808.
Gjødsbøl, K., Christensen, J. J., Karlsmark, T., Jørgensen, B., Klein, B. M., & Krogfelt, K. A. (2006). Multiple bacterial species reside in chronic wounds: a longitudinal study. International wound journal, 3(3), 225-231. DOI: 10.1111/J.1742-481X.2006.00159.X.
Gonelimali, F. D., Lin, J., Miao, W., Xuan, J., Charles, F., Chen, M., & Hatab, S. R. (2018). Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Frontiers in microbiology, 9, 1639. DOI: 10.3389/FMICB.2018.01639/BIBTEX.
Henley-Smith, C.J., Steffens, F.E., Botha, F.S. et al. Predicting the influence of multiple components on microbial inhibition using a logistic response model - a novel approach. BMC Complement Altern Med, 14(1), pp. 1–10. doi: 10.1186/1472-6882-14-190/TABLES/14.
Hussain, T., Arshad, M., Sarzamin, K., Sattar, H., & Qureshi, M. (2011). In Vitro Screening of Methanol Plant Extracts For Their Antibacterial Activity. Pakistan Journal of Botany, 43(1), 531-538.
Irawaty F. (2017). Uji Aktivitas antibakteri ekstrak rebusan ramuan obat pahit warisan budaya Melayu Lingga Kepulauan Riau terhadap bakteri penyebab diare. Unpublished thesis in partial fulfillment of the requirements for the degree of Bachelor of Science, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau.
Kaper, J. B., Nataro, J. P. and Mobley, H. L. T. (2004) ‘Pathogenic Escherichia coli’, Nature reviews. Microbiology, 2(2), pp. 123–140. DOI: 10.1038/NRMICRO818.
Khan, U. A., Rahman, H., Niaz, Z., Qasim, M., Khan, J., Tayyaba, & Rehman, B. (2013). Antibacterial activity of some medicinal plants against selected human pathogenic bacteria. European Journal of Microbiology and Immunology, 3(4), 272-274. DOI: 10.1556/EUJMI.3.2013.4.6.
Körber, A., Schmid, E. N., Buer, J., Klode, J., Schadendorf, D., & Dissemond, J. (2010). Bacterial colonization of chronic leg ulcers: current results compared with data 5 years ago in a specialized dermatology department. Journal of the European Academy of Dermatology and Venereology, 24(9), 1017-1025. DOI: 10.1111/J.1468-3083.2010.03570.X.
Lim, J. Y., Yoon, J. W. and Hovde, C. J. (2010) ‘A Brief Overview of Escherichia coli O157:H7 and Its Plasmid O157’, Journal of microbiology and biotechnology, 20(1), p. 5. DOI: 10.4014/jmb.0908.08007.
Nataro, J. P. and Kaper, J. B. (1998) ‘Diarrheagenic Escherichia coli’, Clinical microbiology reviews, 11(1), pp. 142–201. DOI: 10.1128/CMR.11.1.142.
Nopiyanti, H. T., & Agustriani, F. (2016). Skrining Nypa fruticans sebagai Antibakteri Bacillus subtilis, Escherichia coli dan Staphylococcus aureus. Maspari Journal: Marine Science Research, 8(2), 83-90. DOI: 10.56064/MASPARI.V8I2.3484.
Nofita SH. (2017). Uji aktivitas antibakteri ekstrak rebusan ramuan obat pahit warisan budaya melayu lingga kepulauan riau terhadap bakteri penyebab penyakit kulit. Unpublished thesis in partial fulfillment of the requirements for the degree of Bachelor of Science, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau.
Rahman Neamah, S., Hadi Mohsin, D. and Hameed Kamil, Z. (2021) ‘Phytochemical Screening and Antibacterial Effect of Methanol Extracts of Suaeda aegyptiaca Leaves on Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa’, Archives of Razi Institute, 76(5), p. 1343. DOI: 10.22092/ARI.2021.356133.1784.
Rossi, R. and Ciofalo, M. (2020) ‘An Updated Review on the Synthesis and Antibacterial Activity of Molecular Hybrids and Conjugates Bearing Imidazole Moiety’, Molecules (Basel, Switzerland), 25(21). DOI: 10.3390/MOLECULES25215133.
Ristoja. (2012). Research of Medicinal plants and herbs at Riau and Riau Islands. Universitas Riau, Pekanbaru. [Indonesian]
Srivastava, J., Chandra, H., Nautiyal, A. R., & Kalra, S. J. (2014). Antimicrobial resistance (AMR) and plant-derived antimicrobials (PDAms) as an alternative drug line to control infections. 3 Biotech, 4(5), 451-460. DOI: 10.1007/S13205-013-0180-Y.
Talib, W. H. and Mahasneh, A. M. (2010) ‘Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine’, Molecules (Basel, Switzerland), 15(3), pp. 1811–1824. DOI: 10.3390/MOLECULES15031811.
Trivedi, U., Parameswaran, S., Armstrong, A., Burgueno-Vega, D., Griswold, J., Dissanaike, S., & Rumbaugh, K. P. (2014). Prevalence of multiple antibiotic resistant infections in diabetic versus nondiabetic wounds. Journal of pathogens, 1–6. doi: 10.1155/2014/173053.
Zaenal Mustopa, A., Umami, R. N. and Melki, D. (2015). Antibacterial Activity Assay Of Mangrove Extracts Against Salmonella Typhi And Listeria Monocytogenes. Jurnal Ilmu dan Teknologi Kelautan Tropis, 7(2), pp. 603–612. doi: 10.29244/JITKT.V7I2.11029.
License
Copyright (c) 2023 Rahma Dhani, Rodesia Mustika Roza, Fitmawati
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.