Antibacterial Activity of Extracts of Wild Mango (Mangifera spp.), Ruellia tuberosa L and Leucobryum sp. Causes of Gangrene in Patients with Diabetes Mellitus
DOI:
10.29303/jbt.v23i1.4339Published:
2023-01-11Downloads
Abstract
Plants are the main medicinal source that exists around the world, due to the content of bioactive compounds such as flavonoids, alkaloids, saponins, polyphenols, tannins, terpenoids, and essential oils. These bioactive compounds have antimicrobial activity. The purpose of this study was to analyze the antibacterial activity of a combination extracts of Sumatran wild mango (M. sumatrana, and M. foetida var. Batu), R. tuberosa L. and Leucobryum sp. against bacteria that cause gangrene wounds in diabetics. Preparation of plant extraction is carried out by the method of maceration using 70% alcohol. Testing of antibacterial activity using the disc paper method 6 mm in diameter at an extract concentration of 10% with seven treatments (P1-P7). The test results obtained the largest inhibitory zone diameter against Staphyococcus aureus ATCC 6538 and S. epidermidis ATCC 12228 both shown in combination II extracts at P7 treatment (2:3:1 ratio), 9.04±1.42 mm and 8.85±0.21 mm, respectively. The results of the inhibitory zone resulting from the combination extract test included criteria of resistance to Staphylococcus. In the positive control, the diameter of the chloramphenicol inhibition zone against S. aureus belongs to the sensitive category, but to S. epidermidis it belongs to the resistant category. Combination of methanol extract from wild mango, R. tuberosa L and Leucobryum sp. has the opportunity as the candidate for natural-based antibiotics to replace synthetic antibiotics.
Keywords:
antibacterial; bioactive compounds; diabetes mellitus; gangrene; wild mangoReferences
Ajayi, E.I.O., Modo, E.U., Kiakubu, O.T & Molehin, O.R. (2019). Diabetes Care and Wound Healing Using Nauclea latifolia, Manihot esculenta, and Other Natural Products. In: Bioactive Food as Dietary Interventions for Diabetes, Watson, R.R. and V.R Preedy, Eds). Books, Elsevier Inc. DOI: https://doi.org/10.1016/B978-0-12-813822-9.00035-7
Asbanu, Y. W. A., Wijayati, N., & Kusumo, E. (2019). Identifikasi Senyawa Kimia Ekstrak Daun Sirsak (Annona muricata L.) dan Uji Aktivitas Antioksidannya dengan Metode DPPH (2, 2-Difenil-1-Pikrilhidrasil). Indonesian Journal of Chemical Science, 8(3), 153-160. URL http://journal.unnes.ac.id/sju/index.php/ijcs
Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants, 4(196), 2167-0412. DOI: 10.4172/2167-0412.1000196
Bouarab-Chibane, L., Forquet, V., Lantéri, P., Clément, Y., Léonard-Akkari, L., Oulahal, N., ... & Bordes, C. (2019). Antibacterial properties of polyphenols: characterization and QSAR (Quantitative structure–activity relationship) models. Frontiers in microbiology, 10, 829. DOI: https://doi.org/10.3389/fmicb.2019.00829
Decroli, E. (2020). Diagnostic of diabetic foot uclear. Fakultas Kedokteran Universitas Andalas. Padang.
Dewatisari, W.F., Rumiyanti, L., & Rakhmawati, I. (2017). Rendemen dan Skrining Fitokimia pada Ekstrak Daun Sanseviera sp. Jurnal Penelitian Pertanian Terapan, 17 (3): 197-202. DOI: http://dx.doi.org/10.25181/jppt.v17i3.336.
Farhadi, F., Khameneh, B., Iranshahi, M. & Iranshahy, M. (2018). Antibacterial activity of flavonoids and their structure–activity relationship: An update review. Phytotherapy Research, 33 (1): 13-40. DOI: https://doi.org/10.1002/ptr.6208
Fitmawati, Khairunnisa, Resida, E., Emrizal & Roza, R.M. (2019). The Secondary Metabolite Diversity Analysis of Three Mangifera foetida L. Varieties Based on Liquid Chromatography-Mass Spectrometry (LC-MS). URICSE Journal of Physics: Conference Series, 1351, 012027 IOP Publishing. DOI:10.1088/1742-6596/1351/1/012027
Fitmawati, F., Resida, E., Kholifah, S. N., Roza, R. M., Almurdani, M., & Emrizal, E. (2020). Phytochemical screening and antioxidant profiling of Sumatran wild mangoes (Mangifera spp.): a potential source for medicine antidegenerative effects. F1000Research, 9.
Fitmawati, Safitri,M., Kholifah , S.N., Emrizal, Roza, R. M. (2021). (Macang) Source of Potent Antiviral Activity of Against Dengue Virus Serotype 2 (Anti DENV2). URICSE. Journal of Physics: Conference Series 2049, 012018 IOP Publishing. DOI:10.1088/1742-6596/2049/1/012018
Fitmawati, Saputra, A., Kholifah, S.N., Resida, E., Roza, R.M. & Emrizal. (2020b). Morphological and Histological Study of White Rats (Rattus norvegicus) Kidney Following the Consumption of Sumatran Wild Mango Extract (Mangifera spp.). Advances in Biological Sciences Research, vol. 14
Gebreyohannes, G., Moges, F., Sahile, S. & Raja, N. (2013). Isolation and Characterization of Potential Antibiotic Producing Actinomycetes from Water and Sediments of Lake Tana, Ethiopia. Asian Pacific Journal of Tropica Biomedicine. Vol. 3(6): 423-435. doi: 10.1016/S2221-1691(13)60092-1
Hoerr, V., Duggan, G. E., Zbytnuik, L., Poon, K. K., Große, C., Neugebauer, U., ... & Vogel, H. J. (2016). Characterization and prediction of the mechanism of action of antibiotics through NMR metabolomics. BMC microbiology, 16(1), 1-14. DOI 10.1186/s12866-016-0696-5.
Intan, A. E. K., Jannah, N., & Septiana, S. (2020). Pharmacological Activities Of Ruelia Tuberosa. Infokes, 10(1), 239-243.
Jawetz, Melnick, & Adelberg’s. (2005). Mikrobiologi Kedokteran. Terjemahan. Penerbit Salemba Medika. URL: http://penerbitsalemba.com
Kopustinskiene, D. M., Jakstas, V., Savickas, A., & Bernatoniene, J. (2020). Flavonoids as anticancer agents. Nutrients, 12(2), 457. DOI: 10.3390/nu12020457
Lopez-Romero, J. C., González-Ríos, H., Borges, A., & Simões, M. (2015). Antibacterial effects and mode of action of selected essential oils components against Escherichia coli and Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine, 2015. DOI: http://dx.doi.org/10.1155/2015/795435
Mateo, E. M., & Jiménez, M. (2022). Silver Nanoparticle-Based Therapy: Can It Be Useful to Combat Multi-Drug Resistant Bacteria?. Antibiotics, 11(9), 1205. DOI: https://doi.org/10.3390/antibiotics11091205
Mitra S., Manna A. & Rai R. (2019). Phytochemical screening and in-vitro antioxidant potential of two ethnomedicinally important mosses of Dicranaceae from Darjeeling hills. Journal of Pharmacognosy and Phytochemistry, 8(1): 649-654.
Mwitari, P.G., Ayeka, P.A., Ondhico, J., Matu, E.N. & Bii, C.C. (2013). Antimicrobial Activity and Probable Mechanisms of Action of Medicinal Plants of Kenya: Withania somnifera, Warbugia ugandensis, Prunus africana and Plectrunthus barbatus. PLoS One, 8(6): 1-9.
Obuekwe, I.S., Okoyomo, E.P. & Anka, U.S. (2020). Effect of Plant Extract Combinations on Some Bacterial Pathogens. J. Appl. Sci. Environ. Manage, 24 (4): 627- 632. DOI: 10.4314/jasem.v24i4.13
Oh, E. & Jeon, B. (2015). Synergistic anti-Campylobacter jejuni activity of fluoroquinolone and macrolide antibiotics with phenolic compounds. Front. Microbiol, 6:1129. DOI: doi:10.3389/fmicb.2015.01129
Oliver, T.I. & Mutluoglu, M. (2022). Diabetic Foot Ulcer. National Library of Medicine. StatPearls (internet). https://www.ncbi.nlm.nih.gov/books/NBK537328/. (Accessed on October 30, 2022)
Poongothai, P., & Rajan, S. (2013). Antibacterial Properties of Mangifera indica flower extracts on Uropathogenic Escherichia coli. International Journal of Current Microbiology and Aplied Science, 2(12), 104-111. URL: http://www.ijcmas.com
Rahayu W.P. & Nurwitri, C.C. (2012). Mikrobiologi Pangan. PT Penerbit IPB Press. Kampus IPB Taman Kencana. ISBN: 978-979-493-430-2.
Ramadhan, M., Sabarudin, A., & Safitri, A. (2019). In vitro anti-microbial activity of hydroethanolic extracts of Ruellia tuberosa L.: eco-friendly based-product against selected pathogenic bacteria. In IOP Conference Series: Earth and Environmental Science, 239(1). IOP Publishing. DOI: 10.1088/1755-1315/239/1/012028
Ramata-Stunda, A., Petriņa, Z., Valkovska, V., Borodušķis, M., Gibnere, L., Gurkovska, E., & Nikolajeva, V. (2022). Synergistic Effect of Polyphenol-Rich Complex of Plant and Green Propolis Extracts with Antibiotics against Respiratory Infections Causing Bacteria. Antibiotics, 11(2), 160. DOI: https://doi.org/10.3390/antibiotics11020160
Riyaphan J., Pham D.C., Leong M.K. & Weng CF. (2021). In silico approaches to identify polyphenol compounds as α-glucosidase and α-amylase inhibitors against type-ii diabetes. Biomolecules, 11(12): 1877. DOI: 10.3390/biom11121877.
Sagar, P.K., Poonam Sharma, P. & Singh, R. (2020). Antibacterial efficacy of different combinations of clove, eucalyptus, ginger, and selected antibiotics against clinical isolates of Pseudomonas aeruginosa. AYU (An International Quarterly Journal of Research in Ayurveda), 41 (2): 124-129.
Savoia, D. (2012). Plant-derived antimicrobial compounds: Alternatives to antibiotics. Future Microbiology, 7(8): 1-10. DOI: 10.2217/fmb.12.68
Shamsudin, N.F. Ahmed, Q.U., Syed Mahmood, S., Shah, S.A.A., Khatib, A., Mukhtar, S., Alsharif, M. A., Parveen, H. & Zakaria, Z. A. (2021). Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules 2022, 27, 1149. DOI: https://doi.org/10.3390/ molecules27041149
Soleimanpour, S. Sedighinia, F.S., Afshar, A.S., Zarif, R. & Ghazvini, K. (2015). Antibacterial activity of Tribulus terrestris and its synergistic effect with Capsella bursa-pastoris and Glycyrrhiza glabra against oral pathogens: an in-vitro study. Avicenna J Phytomed, 5 (3): 210-217. URL: https://www.researchgate.net/publication/277510229
Spera, A. M., Esposito, S., & Pagliano, P. (2019). Emerging antibiotic resistance: carbapenemase-producing enterobacteria. Bad new bugs, still no new drugs. Le Infezioni in Medicina, 27(4), 357-364. URL: https://europepmc.org/article/med/31846984
Sutrisno, E., Adnyana, I. K., Sukandar, E. Y., Fidrianny, I., & Lestari, T. (2014). Kajian aktivitas penyembuhan luka dan antibakteri binahong (Anredera cordifolia (Ten.) Steenis, pegagan (Centella asiatica (L.) Urban) serta kombinasinya terhadap bakteri Staphylococcus aureus dan Pseudomonas aeruginosa dari pasien luka kaki diabetes. Bionatura-Jurnal Ilmu-ilmu Hayati dan Fisika, 16, 78-82. URL: http://jurnal.unpad.ac.id/bionatura/article/view/7567
Ullah, R., Ibrar, M., Hameed, I., & Hussain, F. (2016). Pharmacognostic and pharmacological evaluation of Ruellia tuberosa L. Pakistan Journal of Pharmaceutical Sciences, 29(6), 2099-2102. URL: https://www.researchgate.net/publication/320890550
Vaou, N., Stavropoulou, E., Voidarou, C., Tsigalou, C. & Bezirtzoglou, E. 2021. Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives. Microorganisms. DOI: https://doi.org/10.3390/microorganisms9102041
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