Biological Agent Trichoderma asperellum and Its in Vitro Inhibitory Activity Against Mango Fruit Rot Pathogens
DOI:
10.29303/jbt.v23i3.4982Published:
2023-05-22Downloads
Abstract
Diseases are one of the major challenges in crop production which can lead to significant economic losses. Ironically, while having multiple negative impacts, synthetic chemical pesticides are considered the main strategy in tackling plant diseases. Trichoderma asperellum is one of the potential fungal species that can inhibit numerous plant diseases. This study reconfirmed the morphological characteristics of Trichoderma isolates obtained and identified it by molecular techniques. In addition, the inhibitory activity of the isolates was tested in vitro against the mango pathogens Colletotrichum asianum and Diaporthe pseudophoenicicola. The results showed that isolate T4 was identified as Trichoderma asperellum. Isolate T4 was able to inhibit C. asianum and D. pseudophoenicicola by 28.19% and 15.64%, respectively. Therefore, this isolate can be used as a biological agent to control plant pathogens in an environmentally friendly, sustainable manner, and as an alternative to synthetic chemical pesticides.
Keywords:
dual culture, morphology, molecular technique, plant disease.References
Anees, M., Azim, R., Ur Rehman, S., Jamil, M., El Hendawy, S. E., & Al-Suhaiban, N. A. (2018). Antifungal potential of Trichoderma strains originated from north western regions of Pakistan against the plant pathogens. Pakistan Journal of Botany, 50(5), 2031–2040.
Bruns, T. D., Lee, S. B., & Taylor, J. W. (1990). White , T . J ., T . D . Bruns , S . B . Lee , and J . W . Taylor . Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics. May 2014.
Ferreira, F. V., & Musumeci, M. A. (2021). Trichoderma as biological control agent: scope and prospects to improve efficacy. World Journal of Microbiology and Biotechnology, 37(5), 1–17. https://doi.org/10.1007/s11274-021-03058-7
He, D. C., He, M. H., Amalin, D. M., Liu, W., Alvindia, D. G., & Zhan, J. (2021). Biological control of plant diseases: An evolutionary and eco-economic consideration. Pathogens, 10(10). https://doi.org/10.3390/pathogens10101311
Islam, S., Siddique, S., Chowdhury, M., & Mishu, N. (2021). Inhibitory Effect of Trichoderma asperellum Isolate against Ralstonia solanacearum Causing Brinjal Wilt. Annals of Bangladesh Agriculture, 24(2), 107–120. https://doi.org/10.3329/aba.v24i2.55788
Ismaiel, M. H., El Zanaty, A. M., & Abdel-Lateif, K. S. (2022). Molecular and Morphological Identification of Trichoderma Isolates From Egyptian Agriculture Wastes-Rich Soil. Sabrao Journal of Breeding and Genetics, 54(3), 598–607. https://doi.org/10.54910/sabrao2022.54.3.12
Katoch, A., & Kapoor, P. (2014). Recent Concepts in Fungal Taxonomy: A Review. Research & Reviews: Journal of Agriculture and Allied Sciences, 3(2), 23–35. http://www.rroij.com/open-access/recent-concepts-in-fungal-taxonomy-a-review.php?aid=33856
Khalid, S. A. (2017). Trichoderma as biological control weapon against soil borne plant pathogens. African Journal of Biotechnology, 16(50), 2299–2306. https://doi.org/10.5897/ajb2017.16270
Li, N., Alfiky, A., Wang, W., & Nourollahi, K. (2018). Volatile Compound-Mediated Recognition and Inhibition Between Trichoderma Biocontrol Agents and Fusarium oxysporum. 9(October), 1–16. https://doi.org/10.3389/fmicb.2018.02614
Liu, X., Min, W., Mei, S., Wang, L., & Jiang, S. (2021). Plant Disease Recognition: A Large-Scale Benchmark Dataset and a Visual Region and Loss Reweighting Approach. IEEE Transactions on Image Processing, 30, 2003–2015. https://doi.org/10.1109/TIP.2021.3049334
Mukhopadhyay, R., & Kumar, D. (2020). Trichoderma: a beneficial antifungal agent and insights into its mechanism of biocontrol potential. Egyptian Journal of Biological Pest Control, 30(1). https://doi.org/10.1186/s41938-020-00333-x
Nurhayati, Y., Suryanti, S., & Wibowo, A. (2021). In Vitro Evaluation of Trichoderma asperellum Isolate UGM-LHAF against Rhizoctonia solani Causing Sheath Blight Disease of Rice. Jurnal Perlindungan Tanaman Indonesia, 25(1), 64. https://doi.org/10.22146/jpti.65290
Pandit, M. A., Kumar, J., Gulati, S., Bhandari, N., Mehta, P., Katyal, R., Rawat, C. D., Mishra, V., & Kaur, J. (2022). Major Biological Control Strategies for Plant Pathogens. Pathogens, 11(2), 1–21. https://doi.org/10.3390/pathogens11020273
Salem-Bango, Z., Price, T. K., Chan, J. L., Chandrasekaran, S., Garner, O. B., & Yang, S. (2023). Fungal Whole-Genome Sequencing for Species Identification: From Test Development to Clinical Utilization. Journal of Fungi, 9(2). https://doi.org/10.3390/jof9020183
Siddiquee, S. (2017). Practical Handbook of the Biology and Molecular Diversityof Trichoderma Species from Tropical Regions, Fungal Biology.
Thavaprakasapandian, R., Raja, M., Kumar, A., & Sharma, P. (2016). Morphological and molecular characterization of Trichoderma asperellum strain Ta13. Indian Phytopathology, 69(3), 298–303.
Tilocca, B., Cao, A., & Migheli, Q. (2020). Scent of a Killer: Microbial Volatilome and Its Role in the Biological Control of Plant Pathogens. Frontiers in Microbiology, 11(February). https://doi.org/10.3389/fmicb.2020.00041
Wang, H., Zhang, R., Duan, Y., Jiang, W., Chen, X., Shen, X., Yin, C., & Mao, Z. (2021). The endophytic strain trichoderma asperellum 6s-2: An efficient biocontrol agent against apple replant disease in china and a potential plant-growth-promoting fungus. Journal of Fungi, 7(12). https://doi.org/10.3390/jof7121050
Zhang, C., Wang, W., Xue, M., Liu, Z., Zhang, Q., Hou, J., Xing, M., Wang, R., & Liu, T. (2021). The combination of a biocontrol agent trichoderma asperellum sc012 and hymexazol reduces the effective fungicide dose to control fusarium wilt in cowpea. Journal of Fungi, 7(9). https://doi.org/10.3390/jof7090685
License
Copyright (c) 2023 Gilang Vaza Benatar, Yeyet Nurhayati, Umi Kulsum
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
