Effect of Garlic Filtrate as An Alternative Anticoagulant for Osmotic Fragility Test (OFT)

Aini; Baiq Isti Hijriani; Fitriani Kusuma Dewi; Bustanul Atfal

doi:10.29303/jbt.v23i1.5623

Effect of Garlic Filtrate as An Alternative Anticoagulant for Osmotic Fragility Test (OFT)

Authors

Aini , Baiq Isti Hijriani , Fitriani Kusuma Dewi , Bustanul Atfal

DOI:

10.29303/jbt.v23i1.5623

Published:

2023-12-06

Issue:

Vol. 23 No. 1 (2023): Special Issue

Keywords:

Anticoagulant, garlic, filtrate, Osmotic fragilitas tes (OFT).

Articles

Downloads

pdf

How to Cite

Aini, Hijriani, B. I., Dewi, F. K., & Atfal, B. (2023). Effect of Garlic Filtrate as An Alternative Anticoagulant for Osmotic Fragility Test (OFT). Jurnal Biologi Tropis, 23(1), 390–395. https://doi.org/10.29303/jbt.v23i1.5623

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Osmotic fragility test (OFT) is an examination conducted to measure the resistance of erythrocytes when exposed to hypotonic saline solutions with various concentrations. The purpose of this study was to analyze the effect of garlic filtrate as an alternative anticoagulant for osmotic fragility test (OFT). The samples used were blood samples with heparin anticoagulant. This study is a pre-experimental study, using NaCl solution with concentrations of 0.2%, 0.3%, 0.35%, and 0.55%. The method used to measure OFT value is spectrophotometric method. The data obtained were analyzed using statistical one way Anova. The results showed the percentage value of hemolysis with garlic filtrate at each concentration was 100%, 99%, 95%, and 5%, respectively. Data analysis using ANOVA showed that there was a significant effect on the effect of garlic filtrate as an alternative anticoagulant on OFT with (p < 0.05) 0.000 < 0.05.

References

Ahmad P, Alvi SS, Salman Khan M. (2019) Functioning of organosulfur compounds from garlic (Allium sativum Linn) in targeting risk factor-mediated at herosclerosis: A cross talk between alternative and modern medicine. Nat Bio-active Compd Vol 1: 561–85. DOI: 10.1007/978-981-13-7154-7_20

Entika R, (2013). Antikoagulan. Program Studi S1 Farmasi Sekolah Tinggi Farmasi Riau. Pekan Baru.

Faudziah. L, (2018). Perbandingan Penggunaan Antikoagulan EDTA dan Filtrat Bawang Putih (Allium Sativum) Sebagai Antikoagulan Alternatif Terhadap Keutuhan Dinding Sel Leukosit, Program Studi D IV Analis Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah.

Hernawan, U. E., & Setyawan, A. D. (2014). Senyawa Organosulfur Bawang Putih (Allium sativum L.) dan Aktivitas Biologinya Aktivitas Biologinya. Biofarmasi Jurusan Biologi FMIPA UNS, 1,64–76. DOI: 10.13057/biofar/f010205

Igbokwe, N. A. (2018). A review of the factors that influence erythrocyte osmotic fragility. Sokoto Journal of Veterinary Sciences, 16(4), 1-23. DOI: 10.4314/SOKJVS.V16I4.1

Imelda, M., & Kurniawan, S. (2013). Peranan garlic (bawang putih) pada pengelolaan hipertensi. CDK-209, 40(10), 746-750.

Kiswari, R. (2014). Hematologi dan Transfusi. jakarta: 2 ed. Jakarta.

Mateen, A. A., Rani, P. U., Naidu, M. U. R., & Chandrashekar, E. (2011). Pharmacodynamic interaction study of Allium sativum (garlic) with cilostazol in patients with type II diabetes mellitus. Indian Journal of Pharmacology, 43(3), 270. DOI: 10.4103/0253-7613.81514

Muslim. Z., Prasetyo. A., & Surya. W. S (2019). Pengaruh Vitamin C terhadap Fragilitas Osmotik Eritrosit pada Mahasiswa Kedokteran Universitas Jember yang Mengalami Stres Psikologis. e-Journal Pustaka Kesehatan, vol. 7 (no.1).

Nuary, A., Aryandi, R., & Makmur, A. S. (2021). Gambaran Morfologi Eritrosit Menggunakan Antikoagulan K3EDTA dan Antikoagulan Filtrat Bawang putih (Allium sativum). Jurnal TLM Blood Smear, 2(2), 48-53. DOI: https://doi.org/10.37362/jmlt.v2i2.735

Nugraha, G. (2017). Panduan Pemeriksaan Laboratorium Hematologi Dasar. Jakarta: Trans info media.

Olas, B. (2019). Anti-aggregatory potential of selected vegetables—Promising dietary components for the prevention and treatment of cardiovascular disease. Advances in Nutrition, 10(2), 280-290. DOI: 10.1093/ADVANCES/NMY085

Rodríguez-Varela, M., García-Rubio, D., De la Mora-Mojica, B., Méndez-Méndez, J., Durán-Álvarez, C., & Cerecedo, D. (2019). Alterations to plasma membrane lipid contents affect the biophysical properties of erythrocytes from individuals with hypertension. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1861(10), 182996. DOI: 10.1016/j.bbamem.2019.05.018.

Virgiati. V., Liliana. A., & Agus. P.W (2017). Perbedaan fragilitas osmotik eritrosit setelah penyimpanan 2 jam, 4 jam, dan 6 jam. Fakultas Kedokteran Universitas Jenderal Achmad Yani.

Salvagno, G. L., Demonte, D., Dima, F., Bovo, C., & Lippi, G. (2020). Stability of refrigerated whole blood samples for osmotic fragility test. Hematology, transfusion and cell therapy, 42, 134-138. DOI: 10.1016/j.htct.2019.06.001

Samuel O. S. (2016). Red blood cell hemolysis during processing. Scientific and Laboratory Services, Pall Corporation, Port Washington, NY. USA. DOI: 10.1111/j.15372995.2011.03298.x

Usman. K, (2020). Medscape. Osmotic fragility of erithyrocite

Warnock LB, Huang D. (2021). Heparin. Encycl Toxicol Third Ed, 837–9. DOI: 10.1016/B978-0-12-386454-3.00733-8

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

Effect of Garlic Filtrate as An Alternative Anticoagulant for Osmotic Fragility Test (OFT)

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow