Fungal Crude Lipase Enzyme Produced Using the SSF (Solid State Fermentation) Method Increases The Washing Test Performance
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Prapti Sedijani , Nida'an Khovia , Dewa Ayu Citra Rasmi , KusmiyatiDOI:
10.29303/jbt.v23i1.6046Published:
2023-11-04Issue:
Vol. 23 No. 1 (2023): Special IssueKeywords:
Biodetergent, fungal isolates (Al and Cl), SSF, Washing Test.Articles
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Abstract
Lipase is an enzyme that commonly used in various industries, including the detergent industry to improve washing performance. Two fungal isolates (Al and Cl) has been reported to be highly active on high pH. This research aims to see the potential of those fungi (crude enzyme) in improving washing performance. Enzymes were produced using the SSF (Solid State Fermentation) method with sugarcane bagasse as a medium. To determine how adding enzymes might affect the effectiveness of washing, studies were conducted. The results show that washing performance increases from 52% (detergent only) to 74% in combination with the crude enzyme. It is concluded that Al has a potential to be involved in biodetergent production. Similar assays with concentrated enzymes are suggested for future studies.
References
Amara, A. A., Salem, S. R., & Shabeb, M. S. A. (2009). The Possibility to Use Bacterial Protease and Lipase as Biodetergent. Global Journal of Biotechnology & Biochemistry, 4(2), 104–114. DOI: https://www.idosi.org/gjbb/gjbb4(2)09/7.pdf
Andaka, G. (2013). Optimasi Konsentrasi Asam Sulfat Dan Kecepatan Pengadukan Pada Proses Hidrolisis Ampas Tebu Menjadi Furfural . Jurnal Teknologi Technoscientia, 5(2), 152–161. URL: https://journal.akprind.ac.id/index.php/technoscientia/article/view/537
Arifiani, N., Norma Ethica, S., Studi, P. D., & Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang, A. (2018). Isolasi Bakteri Penghasil Lipase dan Protease yang Berpotensi sebagai Agen Bioremediasi dari Limbah Biomedis Cair Puskesmas Halmahera Kota Semarang. In Prosiding Seminar Nasional Mahasiswa Unimus (Vol. 1). URL: https://prosiding.unimus.ac.id/index.php/mahasiswa/article/view/156
Cordova, J., Nemmaoui, M., Ismaıli-Alaoui, M., Morin, A., Roussos, S., Raimbault, M., & Benjilali, B. (1998). Lipase production by solid state fermentation of olive cake and sugar cane bagasse. In Journal of Molecular Catalysis B: Enzymatic (Vol. 5). URL: http://hdl.handle.net/10625/18609
E. Sirisha, N. Rajasekar, & M. Lakshmi Narasu. (2010). Isolation and Optimization of Lipase Producing Bacteria from Oil Contaminated Soils. Advances in Biological Research, 4(5), 249–252. URL: https://www.idosi.org/abr/4(5)/2.pdf
Falony, G., Coca Armas, J., Mendoza, J. C. D., & Martínez Hernández, J. L. (2006). Production of Extracellular Lipase from Aspergillus niger by Solid-State Fermentation. Biotechnol, 44(2), 235–240. URL: https://hrcak.srce.hr/file/161921
FAO. (2015). FAO Statistical Pocketbook 2015: World food and agriculture. In Food and Agriculture Organization of the United Nations. URL: https://www.fao.org/documents/card/en/c/cc2211en
Firdaus, Dali, S., & Rusman, H. J. (2017). Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil. Indo. J. Chem. Res., 5(1), 32–36. DOI: https://doi.org/10.30598//ijcr.2017.5-fir
Hasan, F., Shah, A. A., & Hameed, A. (2006). Industrial applications of microbial lipases. Enzyme and Microbial Technology, 39(2), 235–251. DOI: https://doi.org/10.1016/j.enzmictec.2005.10.016
Hasan, F., Shah, A., Javed, S., & Hameed, A. (2010). Enzymes used in detergents: Lipases. African Journal of Biotechnology, 9(31), 4836–4844. DOI: 10.5897/AJBx09.026
Jurado, E., Bravo, V., Luzón, G., Fernández-Serrano, M., García-Román, M., Altmajer-Vaz, D., & Vicaria, J. M. (2007). Hard-surface cleaning using lipases: Enzyme-surfactant interactions and washing tests. Journal of Surfactants and Detergents, 10(1). DOI: https://doi.org/10.1007/s11743-006-1009-z
Layly, I. R., & Nita Oktavia Wiguna. (2016). Studi Potensi Lipase Alcaligenes faecalis Untuk Aplikasi Biodeterjen. Bioteknologi & Biosains Indonesia, 3(2). Diakses dari: URL: http://ejurnal.bppt.go.id/index.php/JBBI
Maftuhah, A. N., Susanti, A., Febrianti, R., Fakultas, A., Universitas, P., Wahab, K. A., Jombang, H., Balai, ), Perbenihan, B., Proteksi, D., Perkebunan, T., Bbpptp, (, & Surabaya, ). (2019). Uji Efektivitas Sifat Antagonisme Lima Isolat Lokal Trichoderma spp. Terhadap Fusarium sp. Agrosaintifika: Jurnal Ilmu-Ilmu Pertanian, 1(1), 1–4. DOI: DOI: https://doi.org/10.32764/agrosaintifika.v1i1.306
Manan, M. A., & Webb, C. (2017). Modern microbial solid state fermentation technology for future biorefineries for the production of added-value products. In Biofuel Research Journal (Vol. 4, Issue 4, pp. 730–740). Green Wave Publishing of Canada. DOI: https://doi.org/10.18331/BRJ2017.4.4.5
M.K.B. Weerasooriya, & A.A.N. Kumarasinghe. (2012). Isolation of alkaline lipase from rubber seed-Partial purification, characterization and its potential applications as a detergent additive. Article in Indian Journal of Chemical Technology, 19, 244–249. URL: https://www.researchgate.net/publication/267366645
Mukherjee, A. K., Adhikari, H., & Rai, S. K. (2008). Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrica grass and potato peel as low-cost medium: Characterization and application of enzyme in detergent formulation. Biochemical Engineering Journal, 39(2). DOI: https://doi.org/10.1016/j.bej.2007.09.017
Ridder, E. R., Nokes, S. E., & Knutson, B. L. (1998). Optimization of solid-state fermentation parameters for the production of xylanase by Trichoderma longibrachiatum on wheat bran. Transactions of the American Society of Agricultural Engineers, 41(5), 1453–1459. DOI: https://doi.org/10.13031/2013.17281
Romdhane, I. B. Ben, Fendri, A., Gargouri, Y., Gargouri, A., & Belghith, H. (2010). A novel thermoactive and alkaline lipase from Talaromyces thermophilus fungus for use in laundry detergents. Biochemical Engineering Journal, 53(1). DOI: https://doi.org/10.1016/j.bej.2010.10.002
Santhosh Kumar, D., & Ray, S. (2014). Fungal Lipase Production by Solid State Fermentation-An Overview. Journal of Analytical & Bioanalytical Techniques, 06(01). DOI: https://doi.org/10.4172/2155-9872.1000230
Sedijani, P., Rasmi, D. A. C., Kusmiyati, K., & Anggriani, R. A. (2021). Powerful Lipolytic Activity of Fungi Isolated from Coconut and Avocado Flesh on Different pH and Temperature. Jurnal Penelitian Pendidikan IPA, 7(SpecialIssue), 365–369. DOI: https://doi.org/10.29303/jppipa.v7ispecialissue.1261
Sharma, R., Chisti, Y., & Banerjee, U. C. (2001). Production, purification, characterization, and applications of lipases. Biotechnology Advances, 19(8), 627–662. DOI: https://doi.org/10.1016/S0734-9750(01)00086-6
Verma N, Thakur S, & Bhatt AK. (2012). Microbial Lipases: Industrial Applications and Properties (A Review). In International Research Journal of Biological Sciences (Vol. 1, Issue 8). www.isca.in
Zahria, I., Nawfa, R., & Zahria, I. (2015). Pemindaian Jamur Kontaminan Ampas Tebu untuk Produksi Enzim Selulase. Jurnal Sains Dan Seni ITS, 4(2). DOI: 10.12962/J23373520.V4I2.13420
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