Callus Induction of Ciangir Passion Fruit (Passiflora sp.) Leaves at Several Concentration Levels of Growth Regulators Substance 2,4-D and BAP
DOI:
10.29303/jbt.v24i1.6454Published:
2024-01-24Downloads
Abstract
Passion fruit plants are important economically due to the high nutrient value of the fruit, and they have an important role in the pharmaceutical world. People often exploit passion fruit plants for medicinal purposes without cultivating them. Passion fruit propagation can be done using seeds, grafting, or cuttings. However, such propagation can increase the value of high genetic variability so that to overcome this can be done by propagating passion fruit seedlings in tissue culture by callus induction. This research was carried out to determine the effect of 2,4-D and BAP on callus induction in passion fruit leaves. The method utilized was a factorial Randomized Group Design (RGD), which consisted of two factors, namely the 2,4-D concentration level (0, 1, 2, 3) ppm and the BAP concentration level (0, 1, 2) ppm, so there were 12 treatment combinations which were repeated three times. The observed parameters were callus area, percentage of browning explants, percentage of living explants, percentage of callus explants, color and texture of callus. The concentration level of 2,4-D 3 ppm and BAP 0 ppm best affected the callus area parameter of 1.96 . The results of this research showed that the percentage of the highest living explants achieved 100%, the percentage of contaminated explants was 0%, the percentage of explants that experienced browning was 66%, and the percentage of explants that experienced callus was 100%, yellowish green in color of callus, and callus texture is crumbly, compact, intermediate. The research should focus on callus induction with other growth regulators.
Keywords:
Auxin, cytokinin, tissue culture, vegetative.References
Anggraeni, D., Ismaini, L., Surya, M. I., Rahmi, H., dan Saputro, N. W. (2022). Inisiasi Kalus Daun Talinum triangulare (Jacq.) Willd Pada Beberapa Kombinasi Konsentrasi Zat Pengatur Tumbuh 2,4-Dichlorophenoxyasetic Acid dan Benzyl Adenine. Jurnal Agrikultura, 33(3), 276-288. DOI: https://doi.org/10.24198/agrikultura.v33i3.40540
Angio, M. H., dan Irawanto, R. (2019). Pendataan Jenis Buah Lokal Indonesia Koleksi Kebun Raya Purwodadi. Jambura Edu Biosfer Journal, 1(2), 41-46. DOI: https://doi.org/10.34312/jebj.v1i2.2476
Astello-garcia, M. G., Robles-martines, M., Rosa, A. P. B. I., dan Santos-diaz, M. d. S. (2012). Establishment of callus from Opuntia robusta Wendl., a wild and medicinal cactus, for phenolic compounds production. African Journal of Biotechnology, 12(21), 3204-3207. DOI: https://www.ajol.info/index.php/ajb/article/download/131811/121413
Azizi, A. A. A., Roostika, I., dan Efendi, D. (2017). Multiplikasi Tunas In Vitro Berdasarkan Jenis Eksplan Pada Enam Genotipe Tebu (Saccharum officinarum L.). Jurnal Littri, 23(2), 90-97. DOI: http://dx.doi.org/10.21082/littri
Cahyono, E. H., Ningsih, R. (2023). Pengembangan Metode Teknik Sterilisasi Eksplan Guna Meningkatkan Keberhasilan Kultur Jaringan Tanaman Stevia (Stevia rebaudiana Bentoni). Jurnal Pengembangan Potensi Laboratorium, 2(2), 60-67. DOI: 10.25047/plp.v2i2.3685
Fachri, H.O., Adriatmoko, W., dan Astuti, P. (2018). Khasiat Ekstrak Buah Markisa Kuning (P. edulis Slims) Sebagai Antiinflamasi Dilihat dari Jumlah Monosit Pada Tikus Wistar Jantan (Rattus norvegicus). Jurnal Stomatognati, 15(2), 34-36. DOI: https://doi.org/10.19184/stoma.v15i2.17930
Farhanah, A., Ashar, J. R., dan Hamzah, P. (2021). Optimalisasi Teknik Isolasi dan Purifikasi DNA Menggunakan Buffer CTAB (Cetyltrimethyl Ammonium Bromide) Pada Tanaman Markisa (Passiflora sp.) Dataran Rendah Kabupaten Jeneponto. Jurnal Agrisistem, 17(1), 31-39. DOI: 10.52625/j-agr.v17i1.191
Huh, Yoon Sun., Joung, Kwan Lee., dan Young, Sang Nam. (2017). Effect of Plant Growth Regulators and antioxidants on in vitro Plant regeneration and callus induction from leaf explants of purple passion fruit (Passifloraa edulis Sims). Journal Plant Biotechnol, 44(1), 335-342. DOI: https://doi.org/10.5010/JPB.2017.44.3.335
Jabbar, F. B. A., Ardiansyah., dan Darsiani. (2020). Pengaruh Pemberian Antibiotik Terhadap Sintasan dan Pertumbuhan Eksplan Rumput Laut Kappaphycus al varezii Secara In Vitro. Jurnal of Fisheries and Marine Science, 2(1), 92-97. DOI: https://doi.org/10.31605/siganus.v2i1.818
Kurningsih, R., Ghazali, M., Rosidah, S., Muspiah, A., Astuti, S.P., dan Nikmatullah, A. (2020). Pelatihan Teknik Dasar Kultur Jaringan Tumbuhan. Jurnal Masyarakat Mandiri, 4(5), 888-896. DOI: https://doi.org/10.31764/jmm.v4i5.3049
Mahadi, I., Syafi’I, W., dan Sari, Y. (2016). Pengaruh Pemberian Hormon 2,4-D dan BAP Terhadap Pertumbuhan Kalus Jeruk Kasturi (Citrus microcarpa). Jurnal Biogenesis, 12 (2), 99-104. DOI: http://dx.doi.org/10.31258/biogenesis.12.2.97-101
Mariamah, Mukarlina, dan Londa, R. (2019). Pertumbuhan Kalus Tanaman Markisa (Passiflora sp.) dengan Penambahan Naphtalane Acetic Acid (NAA) dan 6-Benzyl Amino Purine (BAP). Jurnal Protobiont, 6 (3), 37-41. DOI: http://dx.doi.org/10.26418/protobiont.v6i2.20801
Muhsin, M. A., dan Iskandar. 2017. Pengembangan dalam Pengolahan Buah Markisa di Kelurahan Pasir Putih Kabupaten Sinjai. Jurnal Pengabdian Masyarakat, 1(1), 15-21. DOI: http://journal.unismuh.ac.id/index.php/jp/index
Natasha, K., dan Restiani, R. 2019. Optimasi Sterilisasi Eksplan Pada Kultur In Vitro Gingseng Jawa (Talium paniculatum). Prosiding Symbion-Prodi Pendidikan Biologi Universitas Ahmad Dahlan, Agustus. 87-95, Prodi Pendidikan Biologi Universitas Ahmad Dahlan, Yogyakarta, pp: 2528-5726. DOI: https://doi.org/10.26555/symbion.3512
Oratmagun, K. M., Pandiangana, D., Kandou, F. E. (2017). Deskripsi Jenis-jenis Kontaminan dari Kultur Kalus Catharantus roseus (L.) G. Don. Jurnal MIPA Unsrat, 6(1), 47-52. DOI: https://doi.org/10.35799/jm.6.1.2017.16154
Rasud., Yulianti., dan Bustaman. (2020). Induksi Kalus Secara In Vitro dari Daun Cengkeh (Syizigium aromaticum L.) dalam Media dengan Berbagai Konsentrasi Auksin. Jurnal Ilmu Pertanian Indonesia, 25(1), 67-72. DOI: 10.18343/jipi.25.1.67
Rosyidah, M., Ratnasari, E., dan Rahayu, Y. S. (2014). Induksi Kalus Daun Melati (Jasminum sambac) dengan Penambahan Berbagai Konsentrasi Dichlorophenoxyacetic Acid (2,4-D) dan 6-Benzylamino Purine (BAP) pada Media MS Secara In Vitro. LenteraBio, 3(1), 147-153. DOI: http://ejournal.unesa.ac.id/index.php/lenterabio
Satriadi, Ogie., Efendi, Darda., dan Sulassih. (2017). Konservasi In Vitro Pisang Kepok Unti Sayang (Musa balbisiana) Melalui Pertumbuhan Minimal Pada Berbagai Media. Jurnal Bul. Agrohorti, 5(1), 27-36. DOI: https://doi.org/10.29244/agrob.v5i1.15888
Sulichantini, E. D. (2015). Produksi Metabolit Sekunder Melalui Kultur Jaringan. Prosiding Seminar Nasional Kefarmasian Ke-1, Juni. 5-6, Universitas Mulawarman, Samarinda, pp: 205-212. DOI: https://doi.org/10.25026/mpc.v1i1.27
Sulichantini, E. D., Nazari, A. P. D., dan Nuansyah, A. (2023). Aplikasi Kombinasi Jenis dan Konsentrasi Antioksidan yang Berbeda Sebagai Penghambat Browning Pada Perbanyakan Pisang Cavendish Secara Kultur Jaringan. Jurnal Agroekoteknologi Tropika Lembab, 5(2), 78-83. DOI: http://dx.doi.org/10.35941/jatl.5.2.2023.9959.78-83
Sundari, L., Siregar, L. A. M., dan Hanafiah, D. S. (2015). Kajian Awal: Respon Eksplan Nodus dalam Inisiasi Tunas Mikro Tanaman Karet (Hevea brasiliensis Muell. Arg.) dalam Medium WPM. Jurnal Online Agroekoteknologi, 3(1), 179-187. DOI: 10.32734/jaet.v3i1.9387
Wijaya, B. E., Dwiyani, R., dan Mayadewi, N. N. A. (2023). Induksi dan Multiplikasi Kalus Eucalyptus sp. pada Berbagai Media Callus Induction Medium (CIM) Secara In Vitro. Agrotrop: Journal on Agriculture Science, 13(1), 76-84. DOI: 10.24843/AJoAS.2023.v13.i01.p07
Wulandari, A. S., Sulistyani, E., dan Agustiani, E. L. (2017). Respon Pertumbuhan Tunas Saninten (Castanopsis argentea (Blume) A.DC.) Terhadap Pemberian Zat Pengatur Tumbuh BAP dan IAA Seacara In Vitro. Jurnal Silvikultur Tropika, 8(3), 208-214. DOI: https://doi.org/10.29244/j-siltrop.8.3.208-214
Yuniardi, Fifit. (2019). Aplikasi Dimmer Switch Pada Rak Kultur Sebagai Pegatur Kebutuhan Intensitas Cahaya Optimum Bagi Tanaman In Vitro. Indonesian Journal of Laboratory, 2(1), 8-13. DOI: https://doi.org/10.22146/ijl.v1i4.52991
Ziraluo, Y. P. B. 2021. Metode Perbanyakan Tanaman Ubi Jalar Ungu (Ipomea batatas poiret) dengan Teknik Kultur Jaringan atau Stek Planlet. Jurnal Inovasi Penelitian, 2(3), 1037-1046. DOI: https://doi.org/10.47492/jip.v2i3.819
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