Antibacterial Activity of Streptomyces sp. NrASA6 Culture Extract Isolated from Nypa Palm Worm Substrate against Escherichia coli, Staphylococcus aureus, and Aeromonas sp. NrBF9
DOI:
10.29303/jbt.v24i1.6469Published:
2024-02-13Downloads
Abstract
Aquaculture is an effort to maintain aquatic commodities aimed at increasing cultivation. Probiotics are one of the components needed to serve as food additives that can prevent pathogenic bacterial infections in animals. One of the Actinomycetes species that has the potential to be used as a probiotic is Streptomyces sp. These species are known to be able to inhibit patogenic bacterial infections. The purpose of this research is to know about the antibacterial activity of the isolates Streptomyces sp. NrASA6 inhibiting the growth of Escherichia coli, Staphylococcus aureus, and Aeromonas sp. NrBF9, as well as the optimal for the test bacteria. The methods used in the antibacterial test are the agar diffusion method with pits and the swabbing method on the surface of medium MHA. The test treatment included incubation on time for Streptomyces sp. NrASA6, divided into 10, 15, 20, and 25 days. The tests were conducted at incubating temperatures of 30 °C and the diameter of the clear zone for 24 and 48 hours. The results of this research were that Streptomyces sp. NrASA6 made slowly for the patogenic bacterial Aeromonas sp. NrBF9 with a diameter of 16.97±1.15 mm for 24 hours, while an inhibition zone did not form on Escherichia coli and Staphylococcus aureus bacteri. The characteristic inhibiton of culture extract 15 days for Streptomyces sp. NrASA6 is bacteriostatic. The incubation period of Streptomyces sp. NrASA6 is influential in the stationary phase, namely 15th days after incubation.
Keywords:
Aquaculture, antimicrobe, Streptomyces sp. NrASA6, nypa palm.References
Abdullah, Almuhardi, I., Antoni, & Rahmawati (2020). Aktivitas Antibakteri Actinomycetes Asal Desa Cempaka Kapuas Hulu Kalimantan Barat Terhadap Enteropatogenik Gastroenteritis. Al-Kauniyah: Jurnal Biologi, 13(1):20-30. DOI: https://doi.org/10.15408/kauniyah.v13i1.11731
Abdullah, Rahmawati, & Kurniatuhadi, R. (2020). Aktivitas Antifungi Ekstrak Isolat Nocardia sp. ATS-4.1Terhadap Jamur Candida albicans InaCC-Y116.Jurnal Ilmu Hayati. 19 (3A): 327-334. DOI: https://doi.org/10.14203/beritabiologi.v19i3A.3868
Afrianto, E. & Liviawaty, E. (2009). Pengendalian Hama dan Penyakit Ikan. Kanisus: Yogyakarta.
Ali, H.H. (2013). Isolation and Identification of Staphylococcus Bacteria from Fish of Fresh Water and Its Antibiotics Sensitivity in Mosul City.Basrah Journal of veterinary Research. 13(1):33-42. DOI: https://doi.org/10.33762/bvetr.2014.88123
Ambarwati (2007). Efektivitas Zat Antibakteri Biji Mimba (Azadirachta indica) untuk Menghambat pertumbuhan Salmonella thyposa dan Staphylococcus aureus. Jurnal Biodiversitas, 8(3): 320-325. DOI: https://doi.org/10.13057/biodiv/d080415
Annalisa, P., Andraea, S., & Monica, M. (2007). Mechanisms of Antibiotic Resistance in Staphylococcus aureus. Journal of Future Microbiology. 2(3): 323-343. DOI: https://doi.org/10.2217/17460913.2.3.323
Aziz, G. (2017). Uji Aktivitas Antibakteri dan Antioksidan dari Ekstrak Etil Asetat Kapang Endofit Daun Tanaman Bakung Rawa (Crinum jagus (J. Thomps) Dandy). Universitas Islam Negeri Syarif Hidayahtullah: Jakarta
Berna, M.G., Campa-Córdova ángel, I., Saucedo, P.E., González, M.C., Marrero, R.M., & Mazón-Suástegui, J.M. (2015). Isolation and in Vitro selection of Actinomycetes Strains as Potential Probiotics for Aquaculture. Journal of Veterinary World, 8(2): 170–176. DOI: https://doi.org/10.14202/vetworld.2015.170-176
Budiyanto, M. & Muhtadi, F. (2012). Peranan Bakteri Actinomycetes dalam Industri Antibiotik, Journal online Biosains. 1:71-85.
Fadilah, A. (2023). Komposisi Jenis dan Potensi Actinomycetes Sebagai Antibakteri yang Diisolasi dari Serasah Nipah (Nypa fruticans) di Muara Sungai Kakap Kabupaten Kubu Raya. Skripsi. Universitas Tanjungpura. Pontianak.
Genilloud, O. (2017). Actinomycetes: Still a Source of Novel Antibiotics. Critical Reviews in Biotechnology. 37(6): 1-25. DOI: https://doi.org/10.1039/c7np00026j
Hardi, E.H. (2018). Bakteri Patogen pada Ikan Air Tawar Aeromonas hydrophila dan Pseudomonas fluorescens. Skripsi. Universitas Mulawarman. Samarinda.
Herlina, Rante, Wahyono, Yosi, Murti, B., & Gemini, A. (2010). Purifikasi dan Karakterisasi Antibakteri dari Actinomycetes Asosiasi Spons Terhadap Bakteri Patogen Resisten. Jurnal Farmasi Indonesia. 21(3): 158-165.
Kurniatuhadi, R., Setyawati, T.R., & Yanti, A.H. (2019). Aktivitas Enzimatik Streptomyces spp. yang Diisolasi dari Usus dan Feses Cacing Nipah (Namalycastis rhodochorde). Pros. SemNas. Peningkatan Mutu Pendidikan. 1(1):125-130.
Lestari, S., Mukarlina, & Kurniatuhadi, R. (2019). Identifikasi dan Deteksi Aktiitas Daya Hambat Bakteri Actinomycetes yang Diisolasi dari Tanah Gambut di Desa Tajok Kayong Kalimantan Barat. Protobiont, 8(1): 13-19. DOI: http://dx.doi.org/10.26418/protobiont.v8i1.30843
Lukistyowati, I., & Kurniasih (2010). Pelacakan Gen Aerolysin dari Aeromonas hydrophila pada Ikan Mas yang Diberi Pakan Ekstrak Bawang Putih. Jurnal Veteriner 13(1): 43- 50.
Martin, V., Dorte, F., & Hanne, I. (2019). Antibiotic Resistance and The MRSA Problem. Journal of American Society for Microbiology 7(2). DOI: https://doi.org/10.1128/microbiolspec.gpp3-0057-2018
Maruka, Safriyanto, S., Gatot, S. & Rostiati D.R. (2017). Identifikasi Cemaran Bakteri Eschericia coli pada Ikan Layang (Decapterus ruselli) Segar di Berbagai Pasar Kota Palu. Jurnal Mitra Sains. 5 (1): 84-89.
Mulyadi & Sulistyani, N. (2013). Aktivitas Cairan Kultur 12 Isolat Actinomycetes terhadap Bakteri Resisten. Jurnal Kesehatan Masyarakat. 7(2): 55-112.DOI: http://dx.doi.org/10.12928/kesmas.v7i2.1043
Nurjasmi, R. & Suryani (2020). Uji Antagonis Actinomycetes terhadap Patogen Colletotrichum capsici Penyebab Penyakit Antraknosa pada Buah Cabai Rawit. Jurnal Ilmiah Respati 11(1): 1–12.
Qiting, L., Ganxing, L., Zheng, G., Yuting, W., Zhongheng, X., Yuxian, R., Qingzhong, Z., Miao, C., Xingqing, Z., & Delin, X. (2022). Application of Potential Probiotic Strain Streptomyces sp. SH5 on anti-Aeromonas Infection in Zebrafish Larvae. Journal of Fish and Shellfish Immunology, 127: 375-385. DOI: https://doi.org/10.1016/j.fsi.2022.06.049
Rahayu, W., Hardi, E.H. & Saptiani, G. (2020). Patogenesitas Bakteri Enterobacteriaceae pada Ikan Zebra (Danio rerio) sebagai Hewan Model. Jurnal Verteriner. 21 (4): 512-518. DOI: https://doi.org/10.19087/jveteriner.2020.21.4.512
Rosa, S.R., Endah, S., Nisma, N. & Achmad, A. (2021). Identifikasi Isolat Streptomyces hygroscopicus INACC A497 sebagai Anti Malaria: Uji Pendahulu. Journal of Biology. 8(2): 115-122.
Safia, A., Hanif, U. & Jizu, Z. (2022). Antimicrobial Drug Resistance against Escherichia coli and Its Harmful Effect on Animal Health. Journal of Veterenary Medicine Science 8(4): 1780–1786. DOI: https://doi.org/10.1002/vms3.825
Setyawati, T.R., Yanti, A.H., and Kurniatuhadi, R. (2020). Pathogenicity Profile of Indigenous Bacetria Isolated from Gastrointestinal Tracts and Fecal Pellets of Nipah Worm (Namalycastis rhodochorde). IOP Conf. Ser.: Earth Environ. Sci. 550 012016. DOI 10.1088/1755-1315/550/1/012016
Susanto, D., Sudrajat & Ruga, R. (2012). Studi Kandungan Bahan Aktif Tumbuhan Meranti Merah (Shorea leprosula Miq) Sebagai Sumber Senyawa Antibakteri. Jurnal Mulawarman Scientifie, 11(2): 181-190.
Vijayakumar, R., Panneerselvam, K., Muthukumar, C., Thajuddin, N., Panneerselvam, A., Saravanamuthu, R. (2012). Optimization of Antimicrobial Production by a Marine Actinomycetes Streptomyces afghaniensis VPTS3-1 Isolated from Palk Strait, East Coast of India. Indian J Microbiol. 52(2):230-9. doi: 10.1007/s12088-011-0138-x
Waluyo, L. (2008). Teknik Analisis Mikrobiologi. UMS Press. Solo.
Yanti, A.H., Setyawati, T.R., & Kurniatuhadi, R. (2019). Composition and Characterizations of Actinomycetes Isolated from Nipah Mangrove Sediment, Gastrointestinal and Fecal Pellets of Nipah Worm (Namalycastis rhodhocorde). IOP Conf. Series: Earth and Environmental Science 550. DOI 10.1088/1755-1315/550/1/012016
License
Copyright (c) 2024 Henno Wisnu Putra, Rikhsan Kurniatuhadi, Tri Rima Setyawati, Ari Hepi Yanti
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
