The Potential of The Entomopathogenic Fungus Beauveria Bassiana (bals) Vuillemin to Control Mealybugs Planococcus spp. Hemiptera: Pseudococcidae
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Lukman Taupiq , I Made Sudantha , A. A. SudharmawanDOI:
10.29303/jbt.v24i1.6514Published:
2024-03-10Issue:
Vol. 24 No. 1 (2024): Januari - MaretKeywords:
Beauveria bassiana, biopesticide, controlling, efficacy, entomopathogenic fungus, mealybug insect (planococcus spp.).Articles
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Abstract
The mealybug insect pest planococcus spp. (Hemiptera: Pseudococcidae) attacks most plants, including food, horticultural, and plantation crops, which directly impacts reducing yields. Continuous control using pesticides made from synthetic chemicals harms environmental sustainability. The use of the entomopathogenic fungus Beauveria bassiana as a pest control for Planococcus spp. can be a long-term, environmentally friendly solution. The Beauveria bassiana fungus can kill all insect stages of various plant pests from all insect pest orders. The efficacy of beauveria bassiana is influenced by various types of enzymes from the secondary metabolites produced, namely: chitinase, protease, amylase, and lipase which function as degraders of the insect integument layer. Efficacy is also influenced by the production of toxins consisting of beauvericin, bassianin, bassiacridin, beauvericin, bassianolide, cyclosporine, oosporein, and tenellin which disrupt the nervous system and kill target insects. The results showed that the application of the Beauveria bassiana fungus was effective in reducing the population of the pest Planococcus spp. and reducing plant damage. The Beauveria bassiana mushroom is an alternative substitute for pesticides made from synthetic chemicals and is very prospective to be used as a biopesticide in controlling the pest Planococcus spp. which attacks agricultural plants and plantations.
References
Adelina, N. S. Studi biologi dan pemberian dosis letal iradiasi gamma 60co terhadap kutu putih planococcus lilacinus cockerell, 1905 hemiptera: pseudococcidae (Bachelor's thesis, Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta).
Ahmed, N. H., & Abd-Rabou, S. M. (2010). Host plants, geographical distribution, natural enemies and biological studies of the citrus mealybug, Planococcus citri (Risso)(Hemiptera: Pseudococcidae). Egyptian Academic Journal of Biological Sciences. A, Entomology, 3(1), 39-47. 10.21608/eajbsa.2010.15207.
Al Khoury, C., Guillot, J., & Nemer, N. (2019). Lethal activity of beauvericin, a Beauveria bassiana mycotoxin, against the two‐spotted spider mites, Tetranychus urticae Koch. Journal of Applied Entomology, 143(9), 974-983. https://doi.org/10.1111/jen.12684
Altinok, H. H., Altinok, M. A., & Koca, A. S. (2019). Modes of action of entomopathogenic fungi. Current Trends in Natural Sciences, 8(16), 117-124.
Andriyani R, Nismah N & Emantis R. (2016). The toxicity of purified isolate of polar extract powder leafs Gliricidia maculata hbr. to cacao mealybug (Planococcus minor maskell ) 3 rd International Wildlife Symposium “ Conserving Sumatran Wildlife Heritage for Sustainable Livelihood ” pp 175–81
Anggarawati, S. H., Santoso, T., & Anwar, R. (2017). Penggunaan Cendawan Entomopatogen Beauveria bassiana (Balsamo) Vuillemin Dan Lecanicillium lecanii (ZIMM) Zare & Gams Untuk Mengendalikan Helopeltis antonii Sign (Hemiptera: Miridae) The Use of Entomopathogenic Fungi Beauveria bassiana (Balsamo) Vuillemin. Journal of Tropical Silviculture, 8(3), 197-202. 10.29244/j-siltrop.8.3.197-202.
Bayu, M. S. Y. I., Prayogo, Y., & Indiati, S. W. (2021). Beauveria bassiana: biopestisida ramah lingkungan dan efektif untuk mengendalikan hama dan penyakit tanaman. Buletin Palawija, 19(1), 41-63. 10.21082/bulpa.v19n1.2021.p41-63.
Behie, S. W., Jones, S. J., & Bidochka, M. J. (2015). Plant tissue localization of the endophytic insect pathogenic fungi Metarhizium and Beauveria. Fungal Ecology, 13, 112-119.
1016/j.funeco.2014.08.001
Borghi, E. J. A., Fornaciari, G., Vieira, M. L., Aguiar, R. L., Holtz, A. M., Verdin Filho, A. C., ... & Carvalho, J. R. D. (2021). Planococcus spp.: behavior and monitoring in conilon coffee crops. 10.25186/.v16i.1820
Cheong, P. C., Glare, T. R., Rostás, M., Haines, S., Brookes, J. J., & Ford, S. (2020). Lack of involvement of chitinase in direct toxicity of Beauveria bassiana cultures to the aphid Myzus persicae. Journal of invertebrate pathology, 169, 107276. 169(2020):107276. 10.1016/j.jip.2019.107276
Chinnadurai S, & Ganesh P. (2013). Optimization process for blastospore production of Beauveria bassiana isolates in poly ethylene glycol (peg) supplemented medium. International Journal of Current Microbiology and Applied Scince 2(11):114-122.
Cito, A., Barzanti, G. P., Strangi, A., Francardi, V., Zanfini, A., & Dreassi, E. (2016). Cuticle‐degrading proteases and toxins as virulence markers of Beauveria bassiana (Balsamo) Vuillemin. Journal of Basic Microbiology, 56(9), 941-948. 10.1002/jobm.201600022
Cutino, I., Benvenuti, C., Mazza, G., Conti, B., Marraccini, D., & Gargani, E. (2023). Efficacy of Beauveria bassiana and Mechanical Traps for the Control of Aclees taiwanensis (Coleoptera: Curculionidae) in Fig Plants. Agriculture, 13(11), 2050. https://doi.org/10.3390/agriculture13112050
Daane, K. M., Almeida, R. P., Bell, V. A., Walker, J. T., Botton, M., Fallahzadeh, M., ... & Zaviezo, T. (2012). Biology and management of mealybugs in vineyards. Arthropod management in vineyards: pests, approaches, and future directions, 271-307. 10.1007/978-94-007-4032-7_12
Daane, K. M., Bentley, W. J., Walton, V. M., Malakar-Kuenen, R., Millar, J. G., Ingels, C. A., ... & Gispert, C. (2006). New controls investigated for vine mealybug. Califo rnia Agriculture, 60(1), 31-38.] 10.3733/ca.v060n01p31
Daniel, J. F., Silva, A. A., Nakagawa, D. H., Medeiros, L. S. D., Carvalho, M. G., Tavares, L. J., ... & Rodrigues-Filho, E. (2017). Larvicidal activity of Beauveria bassiana extracts against Aedes aegypti and identification of beauvericins. Journal of the brazilian chemical society, 28, 1003-1013. https://doi.org/10.21577/0103-5053.20160253
Dannon, H. F., Dannon, A. E., Douro-Kpindou, O. K., Zinsou, A. V., Houndete, A. T., Toffa-Mehinto, J., ... & Tamò, M. (2020). Toward the efficient use of Beauveria bassiana in integrated cotton insect pest management. Journal of Cotton Research, 3, 1-21. 10.1186/s42397-020-00061-5
De Oliveira, D. G. P., Lopes, R. B., Rezende, J. M., & Delalibera Jr, I. (2018). Increased tolerance of Beauveria bassiana and Metarhizium anisopliae conidia to high temperature provided by oil-based formulations. Journal of Invertebrate Pathology, 151, 151-157. 0.1016/j.jip.2017.11.012
Dinesh, A. S., & Venkatesha, M. G. G. (2011). Predation of the apefly, Spalgis epius (Lepidoptera: Lycaenidae) on citrus mealybug, Planococcus citri (Hemiptera: Pseudococcidae). Biocontrol Science and Technology, 21(5), 523-533. 10.1080/09583157.2011.559533
Erdoğan, O., & Sağlan, Z. (2023). In Vitro Compatibility of Entomopathogenic Fungi Beauveria Bassiana (Bals.) Vuill. with Different Fungicides. Black Sea Journal of Agriculture, 6(4), 416-421. 10.47115/bsagriculture.1301874.
Fan, Y., Pei, X., Guo, S., Zhang, Y., Luo, Z., Liao, X., & Pei, Y. (2010). Increased virulence using engineered protease-chitin binding domain hybrid expressed in the entomopathogenic fungus Beauveria bassiana. Microbial pathogenesis, 49(6), 376-380. 10.1016/j.micpath.2010.06.013
Fernandes, M. H. D. A., Oliveira, J. E. D. M., Costa, V. A., & Menezes, K. O. D. (2016). Coccidoxenoides perminutus parasitizing Planococcus citri on vine in Brazil. Ciência Rural, 46, 1130-1133. https://doi.org/10.1590/0103-8478cr20150357
Francis, A. W., Kairo, M. T., Roda, A. L., Liburd, O. E., & Polar, P. (2012). The passionvine mealybug, Planococcus minor (Maskell)(Hemiptera: Pseudococcidae), and its natural enemies in the cocoa agroecosystem in Trinidad. Biological Control, 60(3), 290-296. 10.1016/j.biocontrol.2011.12.002
Franco, J. C., Suma, P., da Silva, E. B., Blumberg, D., & Mendel, Z. (2004). Management strategies of mealybug pests of citrus in Mediterranean countries. Phytoparasitica, 32, 507-522. 10.1007/BF02980445.
Franco, J. C., Zada, A., & Mendel, Z. (2009). Novel approaches for the management of mealybug pests. Biorational control of arthropod pests: application and resistance management, 233-278. 10.1007/978-90-481-2316-2_10
Godfrey, K., Ball, J., Gonzalez, D., & Reeves, E. (2003). Biology of the vine mealybug in vineyards in the Coachella Valley, California. Southwestern entomologist, 28(3), 183–196
Gouli, V., Gouli, S., & Kim, J. S. (2014). Production of Beauveria bassiana air conidia by means of optimization of biphasic system technology. Brazilian Archives of Biology and Technology, 57, 571-577. 10.1590/S1516-8913201401745
Indriati G & Samsudin. (2018). Potensi asap cair sebagai insektisida nabati pengendali penggerek buah kopi Hypothenemus hampei. J. Tanam. Ind. dan Penyegar, 5 123–34. 10.21082/JTIDP.V5N3.2018.P123-134
Jaber, L. R., & Ownley, B. H. (2018). Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens?. Biological control, 116, 36-45. https://doi.org/10.1016/j.biocontrol.2017.01.018
Karamaouna, F., Kimbaris, A., Michaelakis, Α., Papachristos, D., Polissiou, M., Papatsakona, P., & Τsora, Ε. (2013). Insecticidal activity of plant essential oils against the vine mealybug, Planococcus ficus. Journal of Insect Science, 13(1), 142. https://doi.org/10.1673/031.013.14201
Kerns D, Wright G., & Loghry J., (2006). Citrus Mealybug Encycl. Entomol. 85721 515–515
Keswani, C., Singh, S. P., & Singh, H. B. (2013). Beauveria bassiana: status, mode of action, applications and safety issues. Biotech Today, 3(1), 16-20. 10.5958/J.2322-0996.3.1.002
Klieber, J., & Reineke, A. (2016). The entomopathogen B eauveria bassiana has epiphytic and endophytic activity against the tomato leaf miner T uta absoluta. Journal of Applied Entomology, 140(8), 580-589. https://doi.org/10.1111/jen.12287
Kumar, V., Singh, G. P., Babu, A. M., Ahsan, M. M., & Datta, R. K. (1999). Germination, penetration, and invasion of Beauveria bassiana on silkworm, Bombyx mori, causing white muscardine. Italian Journal of Zoology, 66(1), 39-43. https://doi.org/10.1080/11250009909356235
Liu, M., Ding, J., & Lu, M. (2023). Influence of symbiotic bacteria on the susceptibility of Plagiodera versicolora to Beauveria bassiana infection. Frontiers in Microbiology, 14, 1290925. 10.3389/fmicb.2023.1290925
Maistrou, S., Paris, V., Jensen, A. B., Rolff, J., Meyling, N. V., & Zanchi, C. (2018). A constitutively expressed antifungal peptide protects Tenebrio molitor during a natural infection by the entomopathogenic fungus Beauveria bassiana. Developmental & Comparative Immunology, 86, 26-33. 10.1016/j.dci.2018.04.015
Mani, M., Krishnamoorthy, A., & Shivaraju, C. (2011). Biological suppression of major mealybug species on horticultural crops in India. Journal of horticultural sciences, 6(2), 85-100. https://doi.org/10.24154/jhs.v6i2.412
Mansour, R., Belzunces, L. P., Suma, P., Zappalà, L., Mazzeo, G., Grissa-Lebdi, K., ... & Biondi, A. (2018). Vine and citrus mealybug pest control based on synthetic chemicals. A review. Agronomy for Sustainable Development, 38, 1-20. https://doi.org/10.1007/s13593-018-0513-7
Mascarin, G. M., & Jaronski, S. T. (2016). The production and uses of Beauveria bassiana as a microbial insecticide. World Journal of Microbiology and Biotechnology, 32, 1-26. 10.1007/s11274-016-2131-3
Meena M, Prasad V, Zehra A, Gupta VK, & Upadhyay RS. (2015). Manitol metabolism during pathogenic fungalhost interactions under stressed conditions. Frontiers in Microbiology 6(2015):1-12. https://doi.org/10.3389/fmicb.2015.01019
Naegele, R. P., Cousins, P., & Daane, K. M. (2020). Identification of Vitis cultivars, rootstocks, and species expressing resistance to a Planococcus mealybug. Insects, 11(2), 86. https://doi.org/10.3390/insects11020086
Naningsi, N. (2019). Penggunaan Mol Hijau Pada Tanaman Kakao Di Desa Dangintukadaya, Kecamatan Jembrana, Kabupaten Jembrana. Jurnal Ilmiah Vastuwidya, 2(2), 16-19. https://doi.org/10.47532/jiv.v2i2.85
Nukmal, N. (2017). Insecticidal effects of the flavonoid-rich fraction of leaves extract of gamal (Gliricidia sepium) on the coffee mealybugs (Planococcus citri Risso.). Annual Research & Review in Biology, 16(6), 1-9. 10.9734/ARRB/2017/36209
Nwilene, F. E., Adeoti, A. O., & Shaibu, A. A. (2023). Efficacy of metarhizium anisopliae and beauveria bassiana as biological control agents of African Rice Gall Midge (AfRGM), Orseolia oryzivora Harris & Gagné. International Journal of Pest Management, 1-8. 10.1080/09670874.2023.2274353
Ortiz-Urquiza, A., & Keyhani, N. O. (2016). Molecular genetics of Beauveria bassiana infection of insects. Advances in Genetics, 94, 165-249. 10.1016/bs.adgen.2015.11.003
Petlamul, W., & Prasertsan, P. (2012). Evaluation of strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the basis of their virulence, germination rate, conidia production, radial growth and enzyme activity. Mycobiology, 40(2), 111-116. 10.5941/MYCO.2012.40.2.111
Purwaningsih, T., Kristanto, B. A., & Karno, K. (2018). Efektifitas aplikasi Beauveria bassiana sebagai upaya pengendalian wereng batang coklat dan walang sangit pada tanaman padi di Desa Campursari Kecamatan Bulu Kabupaten Temanggung. Journal of Agro Complex, 2(1), 12-18. 10.14710/joac.2.1.12-18.
Puspitasari, M., Susilawati, S., Hapsari, A. D., & Harni, R. (2023). Mealybug (Planococcus spp. Hemiptera: Pseudococcidae) as a pest on plantation crops and its control techniques: A review. In IOP Conference Series: Earth and Environmental Science (Vol. 1133, No. 1, p. 012032). IOP Publishing. 10.1088/1755-1315/1133/1/012032.
Rännbäck, L. M., Cotes, B., Anderson, P., Rämert, B., & Meyling, N. V. (2015). Mortality risk from entomopathogenic fungi affects oviposition behavior in the parasitoid wasp Trybliographa rapae. Journal of Invertebrate Pathology, 124, 78-86. 10.1016/j.jip.2014.11.003.
Reineke, A., & Thiéry, D. (2016). Grapevine insect pests and their natural enemies in the age of global warming. Journal of Pest Science, 89(2), 313-328. https://doi.org/10.1007/s10340-016-0761-8
Rosmiati, A., Hidayat, C., Firmansyah, E., & Setiati, Y. (2018). Potensi Beauveria bassiana sebagai agens hayati Spodoptera litura Fabr. pada tanaman kedelai. Agrikultura, 29(1), 43-47. 10.24198/agrikultura.v29i1.16925.
Saranraj, P., & Jayaparakash, A. (2017). Agrobeneficial entomopathogenic fungi–Beauveria bassiana: A review. Indo-Asian Journal of Multidisciplinary Research, 3(2), 1051-1087. 10.22192/iajmr.2017.3.2.4
Sarjan, M., & Nikmatullah, A. (2021, March). Population and attack intensity of Leaf Sucking pests during plantation initiation of three white potato (Solanum tuberosum L.) varieties in medium latitude, East Lombok. In IOP Conference Series: Materials Science and Engineering (Vol. 1098, No. 4, p. 042106). IOP Publishing. 10.1088/1757-899X/1098/4/042106
Sirisena, U. G. A. I., Watson, G. W., Hemachandra, K. S., & Wijayagunasekara, H. N. P. (2013). Mealybugs (Hemiptera: Pseudococcidae) species on economically important fruit crops in Sri Lanka. https://tar.sljol.info/articles/10.4038/tar.v25i1.8031
Soesanthy, F., & Hapsari, A. D. (2022). Efficacy of some plant extracts against mealybugs on cacao. In IOP Conference Series: Earth and Environmental Science (Vol. 974, No. 1, p. 012095). IOP Publishing. 10.1088/1755-1315/974/1/012095
Soetopo, D., & Indrayani, I. G. A. A. (2015). Jamur entomopatogen Beauveria bassiana: potensi dan prospeknya dalam pengendalian hama tungau. Perspektif: Review Penelitian Tanaman Industri, 8(2), 65-73. 10.21082/p.v8n2.2009.%p.
Ugine, T. A. (2011). The effect of temperature and exposure to Beauveria bassiana on tarnished plant bug Lygus lineolaris (Heteroptera: Miridae) population dynamics, and the broader implications of treating insects with entomopathogenic fungi over a range of temperatures. Biological Control, 59(3), 373-383. 10.1016/j.biocontrol.2011.08.004
Vidya, C. V., & Haseena Bhaskar, H. B. (2017). Scymnini (Coleoptera: Coccinellidae) associated with major sucking pests of Kerala. https://doi.org/10.18311/jbc/2017/18618
Vikhe, A. G., Dale, N. S., Umbarkar, R. B., Labade, G. B., Savant, A. R., & Walunj, A. A. (2016). In vitro and in vivo induction, and characterization of toxins isolated from Beauveria bassiana. International Journal of Pure & Applied Bioscience, 4(3), 97-103.
Vinayaga Moorthi, P., Balasubramanian, C., Selvarani, S., & Radha, A. (2015). Efficacy of sub lethal concentration of entomopathogenic fungi on the feeding and reproduction of Spodoptera litura. Springerplus, 4, 1-12. 10.1186/s40064-015-1437-1
Wiryadiputra, S. (2006). Effectiveness of Biopesticide Derived fromCassia spectabilis and Nicotiana tabacum Leaves Against the Main Insect Pests of Coffee and Its Effect On Other Arthropods. Pelita Perkebunan (a Coffee and Cocoa Research Journal), 22(1). 10.22302/iccri.jur.pelitaperkebunan.v22i1.27
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