The Differences on Cholesterol Levels was Centrifuged Samples after Freezed for 30 Minutes and 2 Hours

Chalista Prarastri; Hari Saktiningsih

doi:10.29303/jbt.v24i2.6929

The Differences on Cholesterol Levels was Centrifuged Samples after Freezed for 30 Minutes and 2 Hours

Authors

Chalista Prarastri , Hari Saktiningsih

DOI:

10.29303/jbt.v24i2.6929

Published:

2024-06-20

Issue:

Vol. 24 No. 2 (2024): April - Juni

Keywords:

2 hours delay, 30 minutes delay, centrigutaion, cholesterol.

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Prarastri, C., & Saktiningsih, H. (2024). The Differences on Cholesterol Levels was Centrifuged Samples after Freezed for 30 Minutes and 2 Hours. Jurnal Biologi Tropis, 24(2), 999–1004. https://doi.org/10.29303/jbt.v24i2.6929

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Abstract

Cholesterol is one type of fat commonly recognized by the public that has a function for the formation of a number of important components. The pre-analytical stage accounts for 68% of errors in the laboratory, one of which is in the sample processing section, namely the delayed serum preparation process, this can affect the results that are not appropriate. The purpose of this study was to determine the difference in cholesterol levels in centrifuged samples after freezing 30 minutes and 2 hours. This type of research is experimental research. This research was conducted at the Clinical Chemistry Laboratory of the National College of Health Sciences. The sampling technique used was quota sampling. The samples used were serum consisting of 2 handling groups from 21 respondents. The study used SPSS 25.0 data analysis with paired T test where previously data normality test was conducted. Data were tested with data normality using the Shapiro-wilk test because the sample was less than 50. The results of the study in the paired T test sig (2-tailed) is 0.014 (p value>0.05) can be concluded that there are differences in cholesterol levels in centrifuged samples after freezing 30 minutes and 2 hours.

References

Adi, N., Jangga, & Faedatul Isma. D. (2019). Perbedaan Kadar Kolesterol Dan Trigliserida Serum Dari Darah Yang Dibekukan Sebelum Disentrifus Dan Yang Langsung Disentrifus. Jurnal Media Analis Kesehatan, 10(2),173-174. https://doi.org/10.32382/mak.v10i2.1315

Arieska, D. I., & Pusponegoro, N. H. (2016). Pendugaan Standard Error dan Confidence Interval Koefisien Gini Dengan Metode Bootstrap: Terapan pada Data Susenas Provinsi Papua Barat Tahun 2013. Jurnal Aplikasi Statistika & Komputasi Statistik, 8(2), 57–66. https://doi.org/10.34123/jurnalasks.v8i2.50

Asrori, A., Hermansyah, H., Edyansyah, E., & Sari, P. M. (2022). Analisis Pemeriksaan Kadar Kolesterol Menurut Waktu Sentrifugasi. Klinikal Sains : Jurnal Analis Kesehatan, 10(1), 17–24. https://doi.org/10.36341/klinikal_sains.v10i1.2344

Atika, H. 2023. Pengaruh Lama Penundaaan Pembuatan Serum Terhadap Kadar Kolesterol. Jurnal Analis Kesehatan, 2(1), 10-11.

Damhuri, P. O., Hartuti, Y., & Ica, M. (2023). Pengaruh Waktu Penundaan Pemeriksaan Terhadap Kadar Kolesterol. Jurnal Sains Dan Teknologi Laboratorium Medik, 9(1), 18–21.

DiaSys. (2016). Diagnostic reagent for quantitative in vitro determination of cholesterol in serum or plasma on photometric systems Order Information. Cholesterol FS, 1–2.

Firdaus, A. J. A., Pramono, D., & Purnomo, W. (2020). Pengembangan Sistem Informasi UPT Kalibrasi Dinas Kesehatan Kabupaten Malang Berbasis WEB. Jurnal Sistem Informasi, Teknologi Informasi, Dan Edukasi Sistem Informasi, 1(1), 23–34. https://doi.org/10.25126/justsi.v1i1.3

Gustira, M. dan I. (Ed.). (2017). Pengantar Laboratorium Medik, Bahan Ajar ATLM (2017th ed.). Kemenkes.

Hardisari, R., & Koiriyah, B. (2016). Gambaran Kadar Trigliserida (Metode Gpo-Pap) Pada Sampel Serum dan Plasma EDTA. Jurnal Teknologi Laboratorium, 1(2), 10-15

Hartini, S., & Suryani, M. E. (2016). Uji Kualitas Serum Simpanan Terhadap Kadar Kolesterol Dalam Darah di Poltekkes Kemenkes Kaltim. Jurnal Ilmiah Manuntung, Volume 2(Nomor 1), 65–69. https://doi.org/10.51352/jim.v2i1.49

Hasan, Z., Arif, M., & Bahrun, U. (2017). Variasi Perlakuan Penanganan Sampel Serum Dan Pengaruhnya Terhadap Hasil Pemeriksaan Kreatinin Darah. JST Kesehatan, 7(1), 72–78.

Inayah, S. N., Tanuwidjaja, S., Rahayu, D., & Kusuma, W. N. (2023). Pengaruh Waktu Sentrifugasi Serum Terhadap Kadar Kolesterol. Jurnal Analisis Kimia STABA, 07(01), 25–29.

Kusumaning Tias, S. (2019). Hubungan Perokok Dengan Kadar Kolesterol Pada Orang Dewasa (Di Dusun Temor Lorong Desa Kebunagung). Angewandte Chemie International Edition, 6(11), 951–952., 5–48.

Listiyana, A. D., Mardiana, M., & Prameswari, G. N. (2013). Obesitas sentral dan kadar kolesterol darah total. Jurnal Kesehatan Masyarakat, 9(1), 37–43. https://doi.org/10.15294/kemas.v9i1.2828

Masy, A., & Indon, B. (2017). Seminar Nasional Biodiversitas. Abstrak Seminar Nasional Masyarakat Biodiversitas Indonesia, 4(5), 117–172.

Muharrami, L. K. (2011). Penentuan Kadar Kolesterol Dengan Metode Kromatografi Gas. Penentuan Kadar Kolesterol Dengan Metode Kromatografi Ga, 28–32.

Nurrahmani, U. dan K. H. Stop: Gejala Penyakit Jantung Koroner, Kolesterol Tinggi, Diabetes Melitus, Hipertensi. In Istana Media. Diakses pada 19 Mei 2014, dari http://www.digilib.unipdu.ac.id/beranda/index.php?p=show_detail&id=4109

Priyo Hastono, S., Tataan, G., & Gedong Tataan, K. (2020). Indeks Masa Tubuh, Usia dan Peningkatan Kolesterol Total. Jurnal Kesehatan Metro Sai Wawai, 13(1), 44–50.

Purbayanti, D. (2015). Pengaruh Waktu Pada Penyimpanan Serum Untuk Pemeriksaan Kolesterol Total. Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Palangkaraya, 1(1), 2–10.

Purnamiati, N. P. (2020). Analisis Kadar Bilirubin Serum Bayi Yang Mengalami Ikterus Neonatus. International Journal of Applied Chemistry Research, 1(2), 26. https://doi.org/10.23887/ijacr.v1i2.28720

Saputri, R. A. (2020). Membandingkan kadar kolestrol total menggunakan metode enzimatik kolorimetrik dan metode point of care testing pada pasien hipertensi. Skripsi, 19–20.

Setiani, A. (2022). Gambaran Faktor Yang Mempengaruhi Kadar Kolesterol Pada Pasien Hiperkolesterolemia Di Rumah Sakit Islam Sultan Agung Semarang. Jurnal Ilmu Keperawatan, 9–10.

Stefhanie Affrianti, & Adelia Febriyossa. (2022). Perbedaan Kadar Kolesterol Total Darah Pada Serum Yang Disentrifus Dan Tidak Disentrifus Di Klinik Ratnasari Medical Centre. Jurnal Medical Laboratory, 1(1), 6–9. https://doi.org/10.57213/medlab.v1i1.2

Subrata Tri Widada1, M. A. M. S. C. C. (2016). Gambaran Perbedaan Kadar Kolesterol Total Metode CHOD-PAP (Cholesterol Oxidase – Peroxsidase Aminoantypirin) Sampel Serum dan Sampel Plasma EDTA. Jurnal Teknologi Laboratorium, 5(1), 121–124. https://doi.org/10.1007/978-90-313-9258-2_37

Vani, T. D. (2022). Perbandingan Hasil Pemeriksaan Kolesterol Total Pada Serum Segar Dan Serum Tunda 6 Hari Pada Suhu 4°C Di Laboratorium Patologi Klinik. Skripsi. https://repository.binawan.ac.id/1936/1/Tlm-2022-Vani Talia Damayanti.pdf

Wahyudiati, D. (2017). Kependidikan mipa. Jakarta: Publisher.

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The Differences on Cholesterol Levels was Centrifuged Samples after Freezed for 30 Minutes and 2 Hours

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