Analysis Antimicrobial Activity of Sumbawa White Honey against Staphylococcus aureus ATCC 25293
Authors
Abiel Dwi Cahya Firdaus Alamsyah , Nurmi Hasbi , Rosyunita RosyunitaDOI:
10.29303/jbt.v24i4.7467Published:
2024-10-07Issue:
Vol. 24 No. 4 (2024): Oktober - DesemberKeywords:
Antibacterial activity, S. aureus ATCC 25293, Sumbawa white honey.Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
Staphylococcus aureus is one of the major bacteria causing health problems in humans, such as skin infections, abscesses, sepsis, pneumonia, endocarditis, and osteomyelitis. Methicillin-Resistant Staphylococcus aureus (MRSA) has been identified since 1990. Consequently, research into alternative antibiotics utilizing natural products is crucial, one of which is honey. Honey is chosen due to its antibacterial properties, including sugars, hydrogen peroxide, phenolic compounds, and others. This study aims to analyze the antibacterial activity of white honey from Sumbawa against S. aureus ATCC 25293. The research method employed was experimental in a laboratory setting using a post-test only control group design. The honey was processed by dilution, and antibacterial testing was conducted using the disc diffusion method at concentrations of 25%, 75%, 100%, with chloramphenicol as positive control and DMSO as negative control. The antibacterial test was repeated five times, and antibacterial activity was assessed by measuring the diameter of inhibition zones around the discs. The results showed inhibition zones for honey at concentrations of 25%, 75%, 100% measuring 1.33 mm, 4.60 mm, and 7.33 mm respectively. Based on these findings, it can be concluded that white honey from Sumbawa exhibits antibacterial activity of weak to moderate strength, but its effectiveness against S. aureus ATCC 25293 is relatively low. Further identification of antibacterial compounds in Sumbawa white honey is recommended for quantitative analysis.
References
Adityarini, D., Suedy, S.W.A. & Darmanti, S. (2020). Kualitas Madu Lokal Berdasarkan Kadar Air, Gula Total dan Keasaman dari Kabupaten Magelang. Buletin Anatomi dan Fisiologi. 5(1): 18–24. DOI: https://doi.org/10.14710/baf.5.1.2020.18-24.
Ahmad-Mansour, N., Loubet, P., Pouget, C., Dunyach-Remy, C., Sotto, A., Lavigne, J.P. & Molle, V. (2021). Staphylococcus aureus toxins: An Update on their Pathogenic Properties and Potential Treatments. Toxins. 13(10): 1–22. DOI: https://doi.org/10.3390/toxins13100677.
Almasaudi, S. (2021). The Antibacterial Activities of Honey. Saudi Journal of Biological Sciences. 28(4): 2188–2196. DOI: https://doi.org/10.1016/j.sjbs.2020.10.017.
Astrini, D., Wibowo, M.S. & Nugrahani, I. (2014). Aktivitas Antibakteri Madu Pahit terhadap Bakteri Gram Negatif dan Gram Positif serta Potensinya Dibandingkan terhadap Antibiotik Kloramfenikol, Oksitetrasiklin dan Gentamisin. Acta Pharmaceutica Indonesia. 39(3 & 4): 75–83. DOI: https://doi.org/10.5614/api.v39i3%20&%204.5252
Davis, W.W. & Stout, T.R. (1971). Disc Plate Method of Microbiological Antibiotic Assay. Applied microbiology. 22(4): 659–665. DOI: https://doi.org/10.1128/aem.22.4.666-670.1971.
Dewi, M.A., Kartasasmita, R.E. & Wibowo, M. singgih (2017). Uji Aktivitas Antibakteri beberapa Madu Asli Lebah Asal Indonesia terhadap Staphylococcus aureus dan Escherichia coli. Kartika-Jurnal Ilmiah Farmasi. 5(1): 7–30. http://www.tjyybjb.ac.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=9987.
Dian, R., Fatimawali & Budiarso, F. (2015). Uji Resistensi Bakteri Escherichia coli Yang Diisolasi dari Plak Gigi terhadap Merkuri Dan Antibiotik Kloramfenikol. Jurnal e-Biomedik, 3(1): 59-63. DOI: https://doi.org/10.35790/ebm.3.1.2015.6607.
Evahelda, Pratama, F. & Santoso, B. (2017). Sifat Fisik dan Kimia Madu dari Nektar Pohon Karet di Kabupaten Bangka Tengah, Indonesia. Agritech. 37(4): 363–368. DOI: https://doi.org/10.22146/agritech.16424.
Fadhmi, Mudatsir & Syaukani, E. (2015). Perbandingan Daya Hambat Madu Seulawah dengan Madu Trumon terhadap Staphylococcus aureus secara In Vitro. BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan. 3(1): 9–14. DOI: https://doi.org/10.22373/biotik.v3i1.986.
Fajar, I., Yudha Perwira, I. & Made Ernawati, N. (2022). Pengaruh Derajat Keasaman (pH) terhadap Pertumbuhan Bakteri Toleran Kromium Heksavalen dari Sedimen Mangrove di Muara Tukad Mati, Bali. Current Trends in Aquatic Science. 6(1): 1–6. https://ojs.unud.ac.id/index.php/CTAS/article/view/72176.
Handayani, T.H., Budiman, M.A., Amalia, R.L.R., Pribadi, A., Elfirta, R.R. & Ferdian, P.R. (2022). Aktivitas Antioksidan, Total Fenolik, dan Total Flavonoid Madu Apis mellifera dari Hutan Akasia (Accacia crassicarpa) Riau, Indonesia dengan Beberapa Perlakuan Pengeringan. Jurnal Biologi Indonesia. 18(2): 231–243. DOI: https://doi.org/10.47349/jbi/18022022/231.
Hasan, A.E.Z., Herawati, H., Purnomo, P. & Amalia, L. (2020). Fisikokimia Madu Multiflora Asal Riau dan Potensinya sebagai Antibakteri Escherichia coli dan Staphylococcus aureus. Chemistry Progress. 13(2): 81–90. DOI: https://doi.org/10.35799/cp.13.2.2020.31594.
Huda, M. (2013). Pengaruh Madu Terhadap Pertumbuhan Bakteri Gram Positif (Staphylococcus aureus) dan Bakteri Gram Negatif (Escherichia coli). Jurnal Analis Kesehatan. 2(2): 250–259. https://eresources.poltekkes-smg.ac.id/storage/journal/Jurnal-Analis-Kesehatan/Vol-2,-No-2-(2013)-JURNAL-ANALIS-KESEHATAN/3cfe467aebb886d2fe97fe22e3f0ced9.pdf
Katrin, D., Idiawati, N. & Sitorus, B. (2015). Uji Aktivitas Antibakteri dari Ekstrak Daun Malek (Litsea graciae vidal) terhadap Bakteri Stapylococcus aureus dan Escherichia coli. Jkk. 4(1): 7–12. https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/11720/
Mahmudah, R., Soleha, T.U. & Ekowati, C. (2013). Identifikasi Methicillin-Resistant Staphylococcus aureus (MRSA) Pada Tenaga Medis Dan Paramedis di Ruang Intensivecare Unit (ICU) dan Ruang Perawatan Bedah Rumah Sakit Umum Daerah Abdul Moeloek. Medical Journal of Lampung University. 2(4): 70–78. https://juke.kedokteran.unila.ac.id/index.php/majority/article/view/64/63.
Mandal, M.D. & Mandal, S. (2011). Honey: Its Medicinal Property and Antibacterial Activity. Asian Pacific Journal of Tropical Biomedicine 1(2): 154–160. DOI: https://doi.org/10.1016/S2221-1691(11)60016-6.
Manguntungi, B., Mustopa, A.Z., Meilina, L., Nurfatwa, M., Vanggy, L.R., Irawan, S., Tamzil, M.S., Aprilian, T., Yulianti, Fidduha, A.S. & Wersian, I.N. (2021). The Profile Analysis of Lactic Acid Bacteria (Lab) from Sumbawa White Honey and Its Potential Producing Antibacterial Compounds. Walailak Journal of Science and Technology. 18(15): 1–12. DOI: https://doi.org/10.48048/wjst.2021.22204.
Nadhilla, N.F. (2014). The Activity of Bacterial Agent of Honey Againts Staphylococcus aureus. Jurnal Kesehatan. 3(7): 94-101. https://www.academia.edu/84357080/THE_ACTIVITY_OF_ANTIBACTERIAL_AGENT_OF_HONEY_AGAINST_Staphylococcus_aureus
Pavlova, T., Dimov, I., Kalevska, T. & Stamatovska, V. (2018). Quality Characteristics of Honey: A Review. Proceedings of University of Ruse. 57 (September), hal. 31–37. https://www.honey.com/files/general/refguide.pdf;
Permana, A., N, M., Zuraida, Z. & Ricardo, V. (2023). Uji Daya Hambat Ekstrak Jintan Hitam (Nigella sativa) terhadap Bakteri Acinetobacter baumannii dengan Metode Dilusi Cair. Anakes : Jurnal Ilmiah Analis Kesehatan. 9(2): 242–250. DOI: https://doi.org/10.37012/anakes.v9i2.1887.
Prabowo, S., Yuliani, Prayitno, Y.A., Lestari, K. & Kusesvara, A. (2019). Penentuan Karakteristik Fisiko-kimia beberapa Jenis Madu menggunakan Metode Konvensional dan Metode Kimia. Journal of Tropical AgriFood. 1(2): 66. DOI: https://doi.org/10.35941/jtaf.1.2.2019.2685.66-73.
Ratulangi, W.R. dan Halid, M. (2023). Quality Analysis Based on Organoleptic Properties, Water Content, and Total Reducing Sugars Content in The Raw Honey (Apis dorsata) and Processed Honey. Jurnal Of Health Science. 16(1): 26–36. DOI: https://doi.org/10.33086/jhs.v16.i01.3091.
Rio, Y.B.P., Djamal, A. & Asterina, A. (2012). Perbandingan Efek Antibakteri Madu Asli Sikabu dengan Madu Lubuk Minturun terhadap Escherichia coli dan Staphylococcus aureus secara In Vitro. Jurnal Kesehatan Andalas. 1(2): 59–62. DOI: https://doi.org/10.25077/jka.v1i2.15.
Saepudin, R., Sutriyono, S. & Saputra, R.O. (2014). Kualitas Madu yang Beredar Di Kota Bengkulu Berdasarkan Penilaian Konsumen dan Uji Secara Empirik. Jurnal Sain Peternakan Indonesia. 9(1): 30–40. DOI: https://doi.org/10.31186/jspi.id.9.1.30-40.
Salsabila, H.A., Suwendar & Fitrianingsih, S.P. (2022). Studi Literatur Aktivitas Senyawa Aktif Antibakteri Madu terhadap Methicillin-Resistant Staphylococcus aureus (MRSA). Bandung Conference Series: Pharmacy. 2(2): 332–337. DOI: https://doi.org/10.29313/bcsp.v2i2.4163.
Syuhriatin (2019). Uji Kemurnian Madu yang Dihasilkan Lebah Spesies Cerana sp. dan Trigona sp. dengan Metode HMF (Hidroksi Methyl Furfural). Avesina, 13(1): 43–49. http://e-journal.unizar.ac.id.
Umam, K., Suharli, L., Manguntungi, B., Kusdianawati & Rimbun, R. (2021). Identifikasi Keanekaragaman Tanaman Bunga sebagai Sumber Pakan Lebah Madu di Kawasan Hutan Desa Batu Dulang, Kecamatan Batu Lanteh, Sumbawa. Majalah Ilmiah Biologi Biosfera : A Scientific Journal. 38(1): 18–23. DOI: https://doi.org/10.20884/1.mib.2021.38.1.1049.
Wahyuni & Karim, S.F. (2020). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kacapiring (Gardenia jasminoides ellis) terhadap Bakteri Streptococcus mutans. Jurnal Sains dan Kesehatan. 2(4). DOI: https://doi.org/10.22216/jsi.v4.
Wulandari, D.D. (2017). Kualitas Madu (Keasaman, Kadar Air, Kadar Gula Pereduksi) Berdasarkan Perbedaan Suhu Penyimpanan. Jurnal Sains Natural, 2(1: 16–22. DOI: https://doi.org/10.31938/jsn.v6i2.160.
Yuliati (2017). Uji Efektivitas Larutan Madu Sebagai Antibakteri Terhadap Pertumbuhan Staphylococcus aureus dan Pseudomonas aeruginosae dengan Metode Disk Diffusion. Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan. 11(1): 7–15. DOI: https://doi.org/10.33533/jpm.v11i1.206.
Yunus, M., Abbas, M. & Bakri, Z. (2019). Uji Daya Hambat Madu Hutan Murni (Meu Depuratum) terhadap Pertumbuhsn Bakteri Staphylococcus aureus. Farmasi, 16(1): 6–12. https://jurnal.uit.ac.id/MFN/article/view/5
Zahra, N.N., Muliasari, H., Andayani, Y. & Sudarma, I.M. (2021). Karakteristik Fisikokimia Ekstrak Madu dan Propolis Trigona Sp. Asal Lombok Utara. Jurnal Agrotek Ummat. 8(1): 7.-14. DOI: https://doi.org/10.31764/jau.v8i1.3826.
License
Copyright (c) 2024 Abiel Dwi Cahya Firdaus Alamsyah, Nurmi Hasbi, Rosyunita Rosyunita
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.