Detection of the oxa-23 Gene, Marker of Carbapenem Resistance in Acinetobacter baumannii Isolates from RSUD Dr. Moewardi Surakarta

Vector Stephen Dewangga; Ardy Prian Nirwana; Ratna Setiyaningrum

doi:10.29303/jbt.v24i4.7598

Detection of the oxa-23 Gene, Marker of Carbapenem Resistance in Acinetobacter baumannii Isolates from RSUD Dr. Moewardi Surakarta

Authors

Vector Stephen Dewangga , Ardy Prian Nirwana , Ratna Setiyaningrum

DOI:

10.29303/jbt.v24i4.7598

Published:

2024-11-08

Issue:

Vol. 24 No. 4 (2024): Oktober - Desember

Keywords:

Acinetobacter baumannii, carbapenem, detection of genes, oxa-23, resistance.

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Dewangga, V. S., Nirwana, A. P., & Setiyaningrum, R. (2024). Detection of the oxa-23 Gene, Marker of Carbapenem Resistance in Acinetobacter baumannii Isolates from RSUD Dr. Moewardi Surakarta. Jurnal Biologi Tropis, 24(4), 868–876. https://doi.org/10.29303/jbt.v24i4.7598

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Abstract

Acinetobacter baumannii is a pathogenic germ that is a major problem in cases of hospital-acquired infections. The mortality rate for cases of infectious diseases caused by A. baumannii reaches 23% in hospitalized patients. The increase in the incidence of A. baumannii infections is in line with the increase in the incidence of resistance to antibiotics. The oxa-23 gene is known to be one of the causes of carbapenem resistance in A. baumannii. This study aims to detect the presence of the oxa-23 gene that causes carbapenem resistance in A. baumannii isolates originating from RSUD Dr. Moewardi Surakarta. The research was carried out in a descriptive observational, using Polymerase Chain Reaction (PCR) and electrophoresis techniques, using 20 clinical isolates of A. baumannii obtained from the RSUD Dr. Moewardi. From this research, results were obtained for 8 clinical isolates of A. baumannii from Dr. Moewardi was detected positively as having the oxa-23 gene, so it can be concluded that 40% of the total samples of A. baumannii isolates from RSUD Dr. Moewardi Surakarta was detected to have the oxa-23 gene which causes carbapenem resistance.

References

Akiba, M., Sekizuka, T., Yamashita, A., Kuroda, M., Fujii, Y., Murata, M., Lee, K., Joshua, D. I., Balakrishna, K., Bairy, I., Subramanian, K., Krishnan, P., Munuswamy, N., Sinha, R. K., Iwata, T., Kusumoto, M., & Guruge, K. S. (2016). Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India. Antimicrobial Agents and Chemotherapy, 60(5), 2972–2980. https://doi.org/10.1128/AAC.01950-15

Alzaref, Mhd., Linosefa, L., & Aliska, G. (2021). Prevalensi Gen OXA-23 pada Isolat Klinis Bakteri Acinetobacter baumanii di RSUP Dr. M. Djamil Padang. Jurnal Ilmu Kesehatan Indonesia, 1(3), 403–411. https://doi.org/10.25077/jikesi.v1i3.113

Aydemir, H., Celebi, G., Piskin, N., Oztoprak, N., Keskin, A. S., Aktas, E., Sumbuloglu, V., & Akduman, D. (2012). Mortality attributable to carbapenem-resistant nosocomial Acinetobacter baumannii infections in a Turkish university hospital. Japanese Journal of Infectious Diseases, 65(1), 66–71.

Brunton, L., Knollman, B., & Hilal-Dandan, R. (2017). Goodman and Gilman’s The Pharmacological Basis of Therapeutics, 13th Edition. McGraw Hill LLC. https://books.google.co.id/books?id=yAg7DwAAQBAJ

Geneaid. (2017). PrestoTM Mini gDNA Bacteria Kit Biotech Ltd. Ver. 02. 10. 17.

Guh, A. Y., Limbago, B. M., & Kallen, A. J. (2014). Epidemiology and prevention of carbapenem-resistant Enterobacteriaceae in the United States. Expert Review of Anti-Infective Therapy, 12(5), 565–580. https://doi.org/10.1586/14787210.2014.902306

Gustawan, I. W., Satari, H. I., Amir, I., & Astrawinata, D. A. (2014). Gambaran Infeksi Acinetobacter baumannii dan Pola Sensitifitasnya terhadap Antibiotik. Sari Pediatri, 16(1), 35–40. https://doi.org/10.14238/sp16.1.2014.35-40

Jeon, B.-C., Jeong, S. H., Bae, I. K., Kwon, S. B., Lee, K., Young, D., Lee, J. H., Song, J. S., & Lee, S. H. (2005). Investigation of a Nosocomial Outbreak of Imipenem-Resistant Acinetobacter baumannii Producing the OXA-23 β-Lactamase in Korea. Journal of Clinical Microbiology, 43(5), 2241–2245. https://doi.org/10.1128/JCM.43.5.2241-2245.2005

Karuniawati, A., Saharman, Y. R., & Lestari, D. C. (2013). Detection of carbapenemase encoding genes in Enterobacteriace, Pseudomonas aeruginosa, and Acinetobacter baumanii isolated from patients at Intensive Care Unit Cipto Mangunkusumo Hospital in 2011. Acta Medica Indonesiana, 45(2), 101–106.

Lemos, E. V, de la Hoz, F. P., Einarson, T. R., McGhan, W. F., Quevedo, E., Castañeda, C., & Kawai, K. (2014). Carbapenem resistance and mortality in patients with Acinetobacter baumannii infection: systematic review and meta-analysis. Clinical Microbiology and Infection : The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 20(5), 416–423. https://doi.org/10.1111/1469-0691.12363

Liakopoulos, A., Miriagou, V., Katsifas, E. A., Karagouni, A. D., Daikos, G. L., Tzouvelekis, L. S., & Petinaki, E. (2012). Identification of OXA-23-producing Acinetobacter baumannii in Greece, 2010 to 2011. Euro Surveillance : Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin, 17(11).

Liu, Q., Li, X., Li, W., Du, X., He, J.-Q., Tao, C., & Feng, Y. (2015). Influence of carbapenem resistance on mortality of patients with Pseudomonas aeruginosa infection: a meta-analysis. Scientific Reports, 5, 11715. https://doi.org/10.1038/srep11715

Ludyasari, A. (2014). Pengaruh suhu annealing pada program PCR terhadap keberhasilan amplifikasi DNA udang jari (Metapenaeus elegan) laguna segara anakan Cilacap Jawa Tengah. Universitas Islam Negeri Maulana Malik Ibrahim.

Mugnier, P. D., Poirel, L., Naas, T., & Nordmann, P. (2010). Worldwide dissemination of the blaOXA-23 carbapenemase gene of Acinetobacter baumannii. Emerging Infectious Diseases, 16(1), 35–40. https://doi.org/10.3201/eid1601.090852

Peleg, A. Y., Seifert, H., & Paterson, D. L. (2008). Acinetobacter baumannii: Emergence of a Successful Pathogen. Clinical Microbiology Reviews, 21(3), 538–582. https://doi.org/10.1128/CMR.00058-07

Pertiwi, N. T. Y., & Budayanti, N. N. S. (2018). Studi Molekuler Gen oxa-23 pada Isolat bakteri Acinetobacter baumannii Resisten terhadap Antibiotik Karbapenem di RSUP Sanglah Denpasar. E-Jurnal Medika, 7(6).

Prakobsrikul, N., Malathum, K., Santanirand, P., Chumnumwat, S., Piebpien, P., & Montakantikul, P. (2019). Correlation between antimicrobial consumption and the prevalence of carbapenem-resistant Escherichia coli and carbapenem-resistant Klebsiella pneumoniae at a university hospital in Thailand. Journal of Clinical Pharmacy and Therapeutics, 44(2), 292–299. https://doi.org/10.1111/jcpt.12791

Primasari, F. S., Puspitasari, I., & Nuryastuti, T. (2022). Prevalensi Bakteri Resisten Karbapenem di RSUP Dr. Sardjito Periode Januari-Agustus 2020. Majalah Farmaseutik, 18(3), 265. https://doi.org/10.22146/farmaseutik.v18i3.65823

Schuertz, K. F., Tuon, F. F., Palmeiro, J. K., Conte, D., Telles, J. P. M., Trevisoli, L. E., & Dalla-Costa, L. M. (2018). Bacteremia and meningitis caused by OXA-23-producing Acinetobacter baumannii - molecular characterization and susceptibility testing for alternative antibiotics. Brazilian Journal of Microbiology : [Publication of the Brazilian Society for Microbiology], 49, 199–204. https://doi.org/10.1016/j.bjm.2018.04.002

Tang, S. S., Apisarnthanarak, A., & Hsu, L. Y. (2014). Mechanisms of β-lactam antimicrobial resistance and epidemiology of major community- and healthcare-associated multidrug-resistant bacteria. Advanced Drug Delivery Reviews, 78, 3–13. https://doi.org/10.1016/j.addr.2014.08.003

Turton, J. F., Ward, M. E., Woodford, N., Kaufmann, M. E., Pike, R., Livermore, D. M., & Pitt, T. L. (2006). The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii. FEMS Microbiology Letters, 258(1), 72–77. https://doi.org/10.1111/j.1574-6968.2006.00195.x

Vardakas, K. Z., Tansarli, G. S., Rafailidis, P. I., & Falagas, M. E. (2012). Carbapenems versus alternative antibiotics for the treatment of bacteraemia due to Enterobacteriaceae producing extended-spectrum -lactamases: a systematic review and meta-analysis. Journal of Antimicrobial Chemotherapy, 67(12), 2793–2803. https://doi.org/10.1093/jac/dks301

Vila, J., Martí, S., & Sánchez-Céspedes, J. (2007). Porins, efflux pumps and multidrug resistance in Acinetobacter baumannii. Journal of Antimicrobial Chemotherapy, 59(6), 1210–1215. https://doi.org/10.1093/jac/dkl509

Yang, Y., Xu, Q., Li, T., Fu, Y., Shi, Y., Lan, P., Zhao, D., Chen, Q., Zhou, Z., Jiang, Y., Peleg, A. Y., & Yu, Y. (2019). OXA-23 Is a Prevalent Mechanism Contributing to Sulbactam Resistance in Diverse Acinetobacter baumannii Clinical Strains. Antimicrobial Agents and Chemotherapy, 63(1). https://doi.org/10.1128/AAC.01676-18

Yang, Z., Wang, P., Song, P., & Li, X. (2020). Carbapenemase OXA-423: A Novel OXA-23 Variant in Acinetobacter baumannii. Infection and Drug Resistance, Volume 13, 4069–4075. https://doi.org/10.2147/IDR.S277364

Yustinadewi, P. D., Yustiantara, P. S., & Narayani, I. (2018). MDR-1 GENE 1199 Variant Primer Design Techniques In Pediatric Patient Buffy Coat Samples With LLA. Metamorfosa: Journal of Biological Sciences, 5(1), 105. https://doi.org/10.24843/metamorfosa.2018.v05.i01.p16

Zhao, Y., Hu, K., Zhang, J., Guo, Y., Fan, X., Wang, Y., Mensah, S. D., & Zhang, X. (2019). Outbreak of carbapenem-resistant Acinetobacter baumannii carrying the carbapenemase OXA-23 in ICU of the eastern Heilongjiang Province, China. BMC Infectious Diseases, 19(1), 452. https://doi.org/10.1186/s12879-019-4073-5

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Detection of the oxa-23 Gene, Marker of Carbapenem Resistance in Acinetobacter baumannii Isolates from RSUD Dr. Moewardi Surakarta

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