Effect of Ecoenzyme Addition on Corn-Based Trichoderma asperellum Formula on Spore Count
DOI:
10.29303/jbt.v25i2.8607Published:
2025-04-22Downloads
Abstract
Trichoderma asperellum is recognized as an effective biocontrol agent and plant growth promoter, playing a vital role in sustainable agriculture. To enhance the efficacy of Trichoderma formulations, ecoenzyme supplementation has been proposed as a promising approach. This study investigated the effect of ecoenzyme supplementation on the spore count of corn-based Trichoderma asperellum formulations. A Completely Randomized Design (CRD) was employed with five treatments and five replicates, and spore counts were assessed under varying concentrations of ecoenzyme (0%, 20%, 40%, 60%, and 80%). The results indicated that a 60% ecoenzyme concentration significantly increased the spore count, reaching a maximum of 252.32 × 10⁶ spores. However, higher concentrations (80%) resulted in a decreased spore count (174.88 × 10⁶), which was similar to that of the control (172.6 × 10⁶), likely due to toxic effects or nutrient imbalances. These findings highlight the importance of selecting the optimal ecoenzyme concentration to maximize the spore count of Trichoderma asperellum, thereby contributing to more effective biocontrol and biofertilizer applications in sustainable agricultural practices.
Keywords:
Biocontrol, corn, ecoenzyme, spore count, sustainable agriculture, Trichoderma asperellum.References
Amanullah, F., & Khan, W. U. D. (2023). Trichoderma asperellum L. coupled the effects of biochar to enhance the growth and physiology of contrasting maize cultivars under copper and nickel stresses. Plants, 12(4), 958.
Chandra, K., et al. (2020). Enhancing growth and sporulation of Trichoderma harzianum using different agricultural wastes. Biocatalysis and Agricultural Biotechnology, 29, 101784. DOI: https://doi.org/10.1016/j.bcab.2020.101784
Dhawi, F., et al. (2021). Valorization of agricultural wastes for sustainable production of Trichoderma-based biofertilizers: A review. Journal of Fungi, 7(12), 1048. DOI: https://doi.org/10.3390/jof7121048
Fu, J., Xiao, Y., Wang, Y. F., Liu, Z. H., Zhang, Y. F., & Yang, K. J. (2021). Trichoderma asperellum alters fungal community composition in saline–alkaline soil maize rhizospheres. Soil Science Society of America Journal, 85(4), 1091-1104. DOI: https://doi.org/10.1002/saj2.20245
Hyundai Motorstudio (2024). 10 Benefits of Eco Enzyme for Agriculture and Health. https://hyundai.motorstudio.co.id/senayan-park/newsrooms/benefits-of-eco-enzyme
Ihtiar, A., Vira, T., Faizsyahrani, L., Anggraini, N., Azuhro, V., Dewi, E., & Nurwahyunani, A. (2023). The Utilization of Household Waste Through Ecoenzymes. International Journal of Humanities, Social Sciences and Business (Injoss), 2(2), 239-49. https://pdfs.semanticscholar.org/5b07/33639dfcdd083af01aa757b4d4485d8e7fdc.pdf
Khan et al. (2023). Trichoderma asperellum Empowers Tomato Plants and Suppresses Fusarium oxysporum Through Priming Responses. Frontiers in Microbiology. https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1140378/full
Kim, Y. H., et al. (2024). The role of Trichoderma in plant disease suppression and growth promotion. Journal of Microbiology, 62(2), 125-135. DOI: https://doi.org/10.1007/s12275-024-3417-2
Maharani, P., Suryanti, S., Joko, T., & Somowiyarjo, S. (2024). Rhizophagus intraradices dan Trichoderma asperellum sebagai bioprotektan dan biofertilizer pada bawang merah TSS. Jurnal Ilmu Pertanian Indonesia, 29(2), 287-297. DOI: https://doi.org/10.18343/jipi.29.2.287
Martinez, E., et al. (2022). Trichoderma spp. application improves the nutritional quality of horticultural crops. Agronomy, 12(3), 654. DOI: https://doi.org/10.3390/agronomy12030654
Reyes, A., et al. (2023). Secondary metabolites produced by Trichoderma spp. and their role in plant disease control. Microorganisms, 11(5), 1187. DOI: https://doi.org/10.3390/microorganisms11051187
Saravanakumar et al. (2019). Co-cultivation of Trichoderma asperellum and Bacillus amyloliquefaciens Improves Wheat Growth and Biocontrol Activity. Frontiers in Microbiology. https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01068/full
Soni, S. K., Manhas, R., Jakhar, Y., Sharma, A., & Soni, R. (2022). Biofertilizers for sustainable agriculture: current trends and future perspective. Genomic, Proteomics, and Biotechnology, 331-356. DOI: https://doi.org/10.1080/01904167.2021.1984683
Singh, V., et al. (2023). Low-cost substrates for Trichoderma production and its application in crop improvement. Biocatalysis and Agricultural Biotechnology, 48, 102637. DOI: https://doi.org/10.1016/j.bcab.2023.102637
Woo, P. C., et al. (2021). Multifaceted beneficial effects of Trichoderma on plant growth and health. Journal of Applied Microbiology, 131(4), 1645-1662. DOI: https://doi.org/10.1111/jam.15147
Xue, L., et al. (2022). Effects of Trichoderma harzianum biofertilizer on growth, yield, and quality of Bupleurum chinense. Frontiers in Microbiology, 13, 1029834. DOI: https://doi.org/10.3389/fmicb.2022.1029834
Zhang, C., et al. (2022). Trichoderma-amended biofertilizer stimulates soil resident Aspergillus population for joint plant growth promotion. Microbiome, 10(1), 116. DOI: https://doi.org/10.1186/s40168-022-01310-2
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