Antibacterial Effectiveness Test of Roselle Flower (Hibiscus sabdariffa L.) Ethanol Extract Against Escherichia coli, Staphylococcus aureus, and Propionibacterium acnes Bacteria
DOI:
10.29303/jbt.v25i1.8765Published:
2025-03-20Downloads
Abstract
Bacterial infections are a growing global health challenge, especially with the increasing cases of antibiotic resistance. This study used a true experimental method with a posttest-only control group design to investigate the antibacterial efficacy of roselle flower (Hibiscus sabdariffa L.) ethanol extract against the bacteria Staphylococcus aureus, Escherichia coli, and Propionibacterium acnes. The maceration method was used to extract the roselle flowers using 70% ethanol as the solvent, and the disc diffusion method was used to test for antibacterial activity at extract concentrations of 30%, 40%, and 50%, using the antibiotic chloramphenicol as a positive control. The results showed that the roselle flower ethanol extract exhibited antibacterial activity against all three test bacteria. The highest inhibition zone against E. coli was found at 50% concentration (9.87 mm), classified as moderate. For S. aureus, the 50% concentration produced the highest inhibition zone (18.13 mm) with strong classification, while 30% and 40% concentrations also demonstrated strong inhibitory effects. For P. acnes, all extract concentrations showed strong inhibitory responses with the highest inhibition zone at 50% concentration. Chloramphenicol antibiotic consistently demonstrated higher antibacterial activity across all test bacteria. Statistical analysis revealed significant differences in inhibitory effectiveness based on extract concentration and bacterial type (p<0.001), with a significant interaction between these two factors (η²=0.605). These findings indicate the potential development of roselle flower ethanol extract as a natural antibacterial agent, although further optimization is needed to enhance its effectiveness.
Keywords:
Antibacterial activity, ethanol extract, Escherichia coli, Staphylococcus aureus, Propionibacterium acnes, Roselle flower.References
Alharbi, A. E., AlHussaini, A. M., & Alshami, I. (2024). A comprehensive review of the antimicrobial effects of Hibiscus species. Cureus, 16(3), e73062. https://doi.org/10.7759/cureus.73062
Almajid, A., Bazroon, A., AlAhmed, A., & Bakhurji, O. (2023). Exploring the health benefits and therapeutic potential of Roselle (Hibiscus sabdariffa) in human studies: A comprehensive review. Cureus, 15(12), e49309. https://doi.org/10.7759/cureus.49309
Apriliantisyah, W., Haidir, I., Rasfayanah, R., Sodiqah, Y., & Said, M. F. M. (2022). Daya hambat ekstrak kunyit (Curcucma domestica Val) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Fakumi Medical Journal: Jurnal Mahasiswa Kedokteran, 2(10), Article 10. https://doi.org/10.33096/fmj.v2i10.127
Aprillia, D., & Az-Zahra, N. P. D. (2024). Deteksi Escherichia coli pada pemeriksaan air limbah. Journal of Medical Laboratory Science Technology, 7(1), 11-18. https://doi.org/10.21070/medicra.v7i1.1785
Davis, W. W., & Stout, T. R. (1971). Disc plate method of microbiological antibiotic assay: I. Factors influencing variability and error. Applied Microbiology, 22(4), 659-665. https://doi.org/10.1128/am.22.4.659-665.1971
Dewi, N. P. K. S., & Santika, I. W. M. (2023). Mekanisme Anti-Hipertensi dari Bunga Rosela (Hibiscus sabdariffa L.) dalam Pengobatan Berbasis Bahan Alam: A Systematic Review. Prosiding Workshop Dan Seminar Nasional Farmasi, 2, 184–195. https://doi.org/10.24843/WSNF.2022.v 2.p15
Fitriyani, N. L., Susilowati, S., Lestari, W., Hairat, U., Prastiwi, D., Pangandaheng, T., Putri, N. M. D., Serinadi, D. M., Daryaswanti, P. I., & Efitra, E. (2023). Patofisiologi. PT. Sonpedia Publishing Indonesia.
Indarto, I., Narulita, W., Anggoro, B. S., & Novitasari, A. (2019). Aktivitas antibakteri ekstrak daun binahong terhadap Propionibacterium acnes. Biosfer: Jurnal Tadris Biologi, 10(1), 67-78. https://doi.org/10.24042/biosfer.v10i1.4102
Khasanah, N., & Rianti, E. D. D. (2024). Pengaruh tinggi konsentrasi propolis terhadap efektivitas daya hambat pada bakteri Staphylococcus aureus. Prosiding Seminar Nasional COSMIC Kedokteran, 2, 198-204.
Mahmudah, F. L., & Atun, S. (2017). Uji aktivitas antibakteri dari ekstrak etanol temu kunci (Boesenbergia pandurata Roxb) terhadap bakteri Streptococcus mutans. Jurnal Penelitian Saintek, 22(1), 59-68. https://doi.org/10.21831/jps.v22i1.15380
Misna, M., & Diana, K. (2016). Aktivitas antibakteri ekstrak kulit bawang merah (Allium cepa L.) terhadap bakteri Staphylococcus aureus. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy), 2(2), 138-144. https://doi.org/10.22487/j24428744.2016.v2.i2.5990
Nuryah, A., Yuniarti, N., & Puspitasari, I. (2019). Prevalensi dan Evaluasi Kesesuaian Penggunaan Antibiotik pada Pasien dengan Infeksi Methicillin Resistant Staphylococcus Aureus di RSUP Dr. Soeradji Tirtonegoro Klaten. Majalah Farmaseutik, 15(2), 123. https://doi.org/10.22146/farmaseutik.v15i2.47911
Pariury, J. A., Herman, J. P. C., Rebecca, T., Veronica, E., & Arijana, I. G. K. N. (2021). Potensi kulit jeruk bali (Citrus maxima Merr) sebagai antibakteri Propionibacterium acne penyebab jerawat. Hang Tuah Medical Journal, 19(1), 119-131. https://doi.org/10.30649/htmj.v19i1.65
Ramdhania, E. Y., Ferasyi, T. R., Sari, W. E., Abrar, M., Ismail, I. I., & Thasmi, C. N. (2020). Angka prevalensi cemaran bakteri Escherichia coli pada daging ayam broiler yang dijual di tiga pasar tradisional Kota Banda Aceh. Jurnal Ilmiah Mahasiswa Veteriner, 4(3), 185-190. https://doi.org/10.21157/jimvet.v4i3.15180
Retnaningsih, A. (2016). Uji daya hambat daun petai cina (Leucaena leucocephala folium) terhadap bakteri Staphylococcus aureus dan Escherichia coli menggunakan metode difusi agar. Jurnal Dunia Kesmas, 5(2), 93-98.
Rivai, H., Asra, R., & Putri, A. (2019). Analisis kualitatif dan kuantitatif kandungan kimia dari ekstrak heksan, aseton, etanol dan air dari kelopak bunga rosela (Hibiscus sabdariffa Linn.). Research Gate. https://doi.org/10.13140/RG.2.2.28840.21768
Sophia, A., Suraini, S., & Pangestu, M. W. (2021). Ekstrak daun jeruk purut (Citrus hystrix D.C) mampu menghambat pertumbuhan Candida albicans. Jurnal Kesehatan Perintis, 8(2), 180-186. https://doi.org/10.33653/jkp.v8i2.643
Umar, F. (2023). Mycobacterium tuberculosis: Kajian mekanisme resistensi intrinsik dan resistensi genetik terhadap obat anti tuberkulosis. PT Pusat Literasi Dunia.
Wahyudi, A., Sapada, I. E., & Agustin, Y. (2024). Aktivitas antibakteri dari ekstrak metanol maggot (Hermetia illucens) terhadap Propionibacterium acnes. Jurnal Kesehatan: Jurnal Ilmiah Multi Sciences, 14(1), 01-10. https://doi.org/10.52395/jkjims.v14i1.408
Wijanarko, B. S., & Cholid, M. A. (2022). Kelopak bunga rosela merah herbal (Hibiscus sabdariffa var. sabdariffa): Sumber minuman penjaga stamina tubuh. PT Kanisius.
World Health Organization. (2023). Antimicrobial resistance. Retrieved February 28, 2025, from https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance
Zahki, M. (2023). Efektifitas antibakteri senyawa metabolit sekunder pada beberapa tanaman obat terhadap pertumbuhan bakteri Staphylococcus aureus. Usadha, 2(2), 25-32. https://doi.org/10.36733/usadha.v2i2.5927
Zamilah, M., Ruhimat, U., & Setiawan, D. (2020). Media alternatif kacang tanah untuk pertumbuhan bakteri. Journal of Indonesian Medical Laboratory and Science (JoIMedLabS), 1(1), 45-51. https://doi.org/10.53699/joimedlabs.v1i1.11
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