Utilization of Black Rice Var Wojalaka with Lemongrass Essential Oil as a Face Mask
Authors
Jennilien Merinda Ghello , Ni Luh Arpiwi , A. A. S. Alit SukmaningsihDOI:
10.29303/jbt.v25i2.8944Published:
2025-05-17Issue:
Vol. 25 No. 2 (2025): April-JuniKeywords:
Antioxidants, black rice, face masks, lemongrass, phytochemicals.Articles
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Abstract
Black rice contains antioxidants that neutralize free radicals, helping prevent wrinkles and skin damage. Fragrant lemongrass produces essential oils with citronellal, geraniol, and citronellol, which have antibacterial effects. This study aims to develop and evaluate a face mask combining black rice extract and citronella essential oil to nourish, cleanse, and tighten the skin. Masks were formulated with black rice concentrations of 30%, 40%, and 50%. Evaluations included phytochemical screening, antioxidant activity (DPPH assay), organoleptic properties, pH, homogeneity, dispersibility, and physical stability. Black rice extract showed antioxidant content of 0.037 mg/L, while the mask formulations exhibited strong antioxidant activity at 2.231 mg/L. The FII mask met Indonesian National Standard (SNI) criteria for physical stability. Clinical tests from week 2 to 6 on volunteers revealed that the FIII mask improved skin firmness, reduced acne and black spots, and increased moisture. This research demonstrates that combining black rice antioxidants with citronella essential oil produces an effective natural face mask that enhances skin health.
References
Annisa, Y. N., & Cahyaningrum, S. E. (2022). Toothpaste Gel Preparation from Composite of Hydroxyapatite-Chitosan-Green Betel Leaf (Piper Batle L.) Extract. Indonesian Journal of Chemical Science, 11(2), 114–122. https://doi.org/10.15294/ijcs.v11i2.54583
Chandimali, N., Bak, S. G., Park, E. H., Lim, H. J., Won, Y. S., Kim, E. K., Park, S. I., & Lee, S. J. (2025). Free radicals and their impact on health and antioxidant defenses: a review. Cell Death Discovery, 11(1). https://doi.org/10.1038/s41420-024-02278-8
Das, A. K., Islam, M. N., Faruk, M. O., Ashaduzzaman, M., & Dungani, R. (2020). Review on tannins: Extraction processes, applications and possibilities. South African Journal of Botany, 135, 58–70. https://doi.org/10.1016/j.sajb.2020.08.008
Das, M., Dash, U., Mahanand, S. S., Nayak, P. K., & Kesavan, R. K. (2023). Black rice: A comprehensive review on its bioactive compounds, potential health benefits and food applications. Food Chemistry Advances, 3(September 2022), 100462. https://doi.org/10.1016/j.focha.2023.100462
de Souza Silva, F. K., Costa-Orlandi, C. B., Fernandes, M. A., Pegorin Brasil, G. S., Mussagy, C. U., Scontri, M., Sasaki, J. C. da S., de Sousa Abreu, A. P., Guerra, N. B., Floriano, J. F., de Mendonça, R. J., Caetano, G. F., Farhadi, N., Gómez, A., Huang, S., Farias, A. M., Primo, F. L., Li, B., Fusco-Almeida, A. M., … Herculano, R. D. (2023). Biocompatible anti-aging face mask prepared with curcumin and natural rubber with antioxidant properties. International Journal of Biological Macromolecules, 242, 124778. https://doi.org/https://doi.org/10.1016/j.ijbiomac.2023.124778
Ghello, J. M. (2025). Uji Fitokimia Oryza sativa L. Wojalaka dari 4 Kabupaten di Pulau Sumba. Journal on Education, 07(02), 11155–11162.
Ghiulai, R., Roşca, O. J., Antal, D. S., Mioc, M., Mioc, A., Racoviceanu, R., Macaşoi, I., Olariu, T., Dehelean, C., Creţu, O. M., Voicu, M., & Şoica, C. (2020). Tetracyclic and pentacyclic triterpenes with high therapeutic efficiency in wound healing approaches. Molecules, 25(23), 1–38. https://doi.org/10.3390/molecules25235557
Hussein, R. S., Bin Dayel, S., Abahussein, O., & El-Sherbiny, A. A. (2024). Influences on Skin and Intrinsic Aging: Biological, Environmental, and Therapeutic Insights. Journal of Cosmetic Dermatology. https://doi.org/10.1111/jocd.16688
Insanu, M., Nayaka, N. M. D. M. W., Solihin, L., Wirasutisna, K. R., Pramastya, H., & Fidrianny, I. (2022). Antioxidant activities and phytochemicals of polar, semi-polar, and nonpolar extracts of used and unused parts of Carica papaya fruit. Biocatalysis and Agricultural Biotechnology, 39, 102270. https://doi.org/https://doi.org/10.1016/j.bcab.2021.102270
Intharuksa, A., Kuljarusnont, S., Sasaki, Y., & Tungmunnithum, D. (2024). Flavonoids and Other Polyphenols: Bioactive Molecules from Traditional Medicine Recipes/Medicinal Plants and Their Potential for Phytopharmaceutical and Medical Application. Molecules, 29(23). https://doi.org/10.3390/molecules29235760
Jamilatun, M., Pratiwi, M. D., & Yulianto, S. (2024). Formulation and Evaluation of Face Mask Gel Peel Off Combination of Avocado Leaf Extract (Perssea Americana Mill) and Jicama (Pachyrrhuzul Erosus L.). Formosa Journal of Science and Technology, 3(8), 1739–1748. https://doi.org/10.55927/fjst.v3i8.10580
Jomova, K., Raptova, R., Alomar, S. Y., Alwasel, S. H., Nepovimova, E., Kuca, K., & Valko, M. (2023). Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. In Archives of Toxicology (Vol. 97, Issue 10). Springer Berlin Heidelberg. https://doi.org/10.1007/s00204-023-03562-9
Kirana, K. (2023). Oral Collagen and Aging: A Review. Healthy Tadulako Journal (Jurnal Kesehatan Tadulako), 9(2), 235–249.
Kollepara, P. K., Siegenfeld, A. F., Taleb, N. N., & Bar-Yam, Y. (2021). Unmasking the mask studies: why the effectiveness of surgical masks in preventing respiratory infections has been underestimated. Journal of Travel Medicine, 28(7), 1–8. https://doi.org/10.1093/jtm/taab144
Lawag, I. L., Nolden, E. S., Schaper, A. A. M., Lim, L. Y., & Locher, C. (2023). A Modified Folin-Ciocalteu Assay for the Determination of Total Phenolics Content in Honey. Applied Sciences (Switzerland), 13(4). https://doi.org/10.3390/app13042135
Liu, Y., Zhu, J., Liu, Z., Zhi, Y., Mei, C., & Wang, H. (2025). Flavonoids as Promising Natural Compounds for Combating Bacterial Infections. International Journal of Molecular Sciences, 26(6), 1–20. https://doi.org/10.3390/ijms26062455
Lukić, M., Pantelić, I., & Savić, S. D. (2021). Towards optimal ph of the skin and topical formulations: From the current state of the art to tailored products. Cosmetics, 8(3). https://doi.org/10.3390/cosmetics8030069
Mohanty, M., Mohanty, J., Dey, S., Dutta, K., Shah, M. P., & Das, A. P. (2024). The face mask: A tale from protection to pollution and demanding sustainable solution. Emerging Contaminants, 10(2), 100298. https://doi.org/10.1016/j.emcon.2023.100298
Mulyaningsih, S., & Rachmadani, F. A. (2022). Determination of the Active Chemical Compounds and the Antibacterial Activity of Various Fractions of Lawsonia inermis L. Borneo Journal of Pharmacy, 5(3), 194–201. https://doi.org/10.33084/bjop.v5i3.3221
Nemati, M. M., Abedi, M., Ghasemi, Y., Ashrafi, H., & Haghdel, M. (2024). Formulation and evaluation of antioxidant and antibacterial activity of a peel-off facial masks moisturizer containing curcumin and Rosa Damascena extract. Journal of Cosmetic Dermatology, 23(6), 2156–2169. https://doi.org/10.1111/jocd.16255
Niah, R., Ariani, N., & Febrianti, D. R. (2022). FORMULASI DAN EVALUASI SEDIAAN HAND WASH MINYAK SERAI WANGI (Cymbopogon nardus L. Rendle). Jurnal Insan Farmasi Indonesia, 5(2), 258–266. https://doi.org/10.36387/jifi.v5i2.1254
Ningsih, A. W., Syahrani, A., Septama, A. W., & Sukardiman. (2024). Metabolit Profilling and Antibacterial Activity of Ethanol Extract of the Unripe Fruit Peel of Kayu Banana (Musa paradisiaca L. Var. Kayu). Trends in Sciences, 21(2). https://doi.org/10.48048/tis.2024.7154
Park, M., Kim, H., Kim, S., Lee, J., Kim, S., Byun, J. W., Hwang-Bo, J., & Park, K. H. (2021). Changes in skin wrinkles and pores due to long-term mask wear. Skin Research and Technology, 27(5), 785–788. https://doi.org/10.1111/srt.13019
Pratiwi, L., & Wijianto, B. (2023). Development of Face Pack Powder of Arabica Coffee (Coffea arabica L.) Using Variations of Binders with D-Optimal Mixture Design. International Journal of Drug Delivery Technology, 13(4), 1290–1296. https://doi.org/10.25258/ijddt.13.4.28
Purwaningsih, I., Fathiah, F., Amaliyah, N., & Kuswiyanto, K. (2023). The Phenolic, Flavonoid, and Anthocyanin Content From Methanol Extract of Senggani Fruit and Its Antioxidant Activity. Indo. J. Chem. Res., 10(3), 195–202. https://doi.org/10.30598//ijcr.2023.10-pur
Rahim, M. A., Umar, M., Habib, A., Imran, M., Khalid, W., Lima, C. M. G., Shoukat, A., Itrat, N., Nazir, A., Ejaz, A., Zafar, A., Awuchi, C. G., Sharma, R., Santana, R. F., & Emran, T. Bin. (2022). Photochemistry, Functional Properties, Food Applications, and Health Prospective of Black Rice. Journal of Chemistry, 2022(1), 2755084. https://doi.org/https://doi.org/10.1155/2022/2755084
Rosaini, H., Makmur, I., Lestari, E. A., Sidoretno, W. M., & Yetti, R. D. (2021). Formulation of Gel Peel Off Catechins Mask from Gambir (Uncaria gambir (Hunter) Roxb) with the PVP K-30 Concentration Variation. International Journal of Research and Review (Ijrrjournal.Com), 8(3), 205–211.
Saptawati, T., Dayanti, E., Rachma, F. A., & Ovikariani. (2022). Phytochemical Screening of Ethanol Extract of Trembesi Fruit Seeds ( Samanea saman ). Science and Community Pharmacy Journal, 1(2), 69–77.
Sari, I., Misrahanum, M., Faradilla, M., Ayuningsih, C. M., & Hilda Maysarah. (2022). Antibacterial Activity of Citronella Essential Oil from Cymbopogon nardus (L.) Rendle) Against Methicillin Resistant Staphylococcus aureus. Indonesian Journal of Pharmaceutical and Clinical Research, 5(1), 16–22. https://doi.org/10.32734/idjpcr.v5i1.8302
Schwinn, D. A., & Jackson, L. M. (2020). Compounded topical pain creams. In Compounded Topical Pain Creams. https://doi.org/10.17226/25689
Shi, L., Zhao, W., Yang, Z., Subbiah, V., & Suleria, H. A. R. (2022). Extraction and characterization of phenolic compounds and their potential antioxidant activities. Environmental Science and Pollution Research, 29(54), 81112–81129. https://doi.org/10.1007/s11356-022-23337-6
Singh, S. P., Vanlalsanga, Mehta, S. K., & Singh, Y. T. (2022). New insight into the pigmented rice of northeast India revealed high antioxidant and mineral compositions for better human health. Heliyon, 8(8), e10464. https://doi.org/10.1016/j.heliyon.2022.e10464
Syakri, S., Syahrana, N. A., Ismail, A., Tahir, K. A., & Masri, A. (2021). A review: Testing antioxidant activity on kawista plants (limonia acidissima l.) in indonesia. Open Access Macedonian Journal of Medical Sciences, 9(F), 281–287. https://doi.org/10.3889/oamjms.2021.6497
Taebenu, D. A. N. T., Arpiwi, N. L., & Sudatri, N. W. (2023). FORMULATION OF SUNSCREEN BODY LOTION FROM MORINGA ( Moringa oleifera L.) LEAF EXTRACT. Jurnal Biosains Pascasarjana, 25(2), 137–145. https://doi.org/10.20473/jbp.v25i2.2023.137-145
Timilsena, Y. P., Phosanam, A., & Stockmann, R. (2023). Perspectives on Saponins: Food Functionality and Applications. International Journal of Molecular Sciences, 24(17). https://doi.org/10.3390/ijms241713538
Wang, M. X., Gwee, S. X. W., Chua, P. E. Y., & Pang, J. (2020). Effectiveness of Surgical Face Masks in Reducing Acute Respiratory Infections in Non-Healthcare Settings: A Systematic Review and Meta-Analysis. Frontiers in Medicine, 7(September). https://doi.org/10.3389/fmed.2020.564280
WUTSQA, Y. U., SURATMAN, S., & SARI, S. L. A. (2021). Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography. Asian Journal of Natural Product Biochemistry, 19(2), 66–69. https://doi.org/10.13057/biofar/f190204
Xu, H., Wu, Z., Zhao, D., Liang, H., Yuan, H., & Wang, C. (2022). Preparation and characterization of electrospun nanofibers-based facial mask containing hyaluronic acid as a moisturizing component and huangshui polysaccharide as an antioxidant component. International Journal of Biological Macromolecules, 214, 212–219. https://doi.org/https://doi.org/10.1016/j.ijbiomac.2022.06.047
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