Potential of Antimicrobial Peptides (AMPs) Extracted from Black Soldier Fly Larvae Against Vibrio parahaemolyticus In Vitro

Riskia Khanifa; Aida Himmatul Ulya; Ahmad Edi Darmawan; Anis H. Mahsunah; Wahyu Aji Mahardhika

doi:10.29303/jbt.v25i4.9655

Potential of Antimicrobial Peptides (AMPs) Extracted from Black Soldier Fly Larvae Against Vibrio parahaemolyticus In Vitro

Authors

Riskia Khanifa , Aida Himmatul Ulya , Ahmad Edi Darmawan , Anis H. Mahsunah , Wahyu Aji Mahardhika

DOI:

10.29303/jbt.v25i4.9655

Published:

2025-10-01

Issue:

Vol. 25 No. 4 (2025): Oktober-Desember

Keywords:

Antimicrobial peptides (AMPs); SDS Page; Vanname shrimp; Vibrio parahaemolyticus

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Khanifa, R., Ulya, A. H., Darmawan, A. E., Mahsunah, A. H., & Mahardhika, W. A. (2025). Potential of Antimicrobial Peptides (AMPs) Extracted from Black Soldier Fly Larvae Against Vibrio parahaemolyticus In Vitro. Jurnal Biologi Tropis, 25(4), 5019–5029. https://doi.org/10.29303/jbt.v25i4.9655

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Abstract

Whiteleg shrimp (Litopenaeus vannamei) is one of the most widely farmed shrimp species; however, its cultivation is frequently threatened by vibriosis caused by Vibrio parahaemolyticus. To date, the potential of antimicrobial peptides (AMPs) derived from Black Soldier Fly (BSF) larvae as natural antimicrobial agents has not been reported. This study aimed to evaluate the effectiveness of BSF larval AMPs against V. parahaemolyticus, the causative agent of vibriosis in L. vannamei. Data were analyzed using a quantitative descriptive approach. The results showed that BSF larval AMPs contained protein levels ranging from 5.2–12.03% in the first trial and 9.19–10.82% in the second trial, while the water content varied between 4.77% and 77.89%. In vitro testing demonstrated that BSF larval AMPs inhibited V. parahaemolyticus growth with a 9 mm inhibition zone in all treatments. Further in vivo studies and optimization are necessary to develop BSF larval AMPs as sustainable, eco-friendly antimicrobial agents.

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Potential of Antimicrobial Peptides (AMPs) Extracted from Black Soldier Fly Larvae Against Vibrio parahaemolyticus In Vitro

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