Growth of Pontianak Siamese Shoot Culture (Citrus nobilis var. microcarpa) Supplemented with Tomato Extract and BAP (6-benzylaminopurine)

Duwi Rida Istiani; Mukarlina Mukarlina; Siti  Ifadatin

doi:10.29303/jbt.v25i4a.9666

Growth of Pontianak Siamese Shoot Culture (Citrus nobilis var. microcarpa) Supplemented with Tomato Extract and BAP (6-benzylaminopurine)

Authors

Duwi Rida Istiani , Mukarlina Mukarlina , Siti Ifadatin

DOI:

10.29303/jbt.v25i4a.9666

Published:

2025-12-21

Issue:

Vol. 25 No. 4a (2025): Special Issue

Keywords:

BAP (6-Benzyl Amino Purine), Citrus nobilis var. Microcarpa, Shoot culture, Tomato extract

Articles

Downloads

pdf

How to Cite

Istiani, D. R., Mukarlina, M., & Ifadatin, S. (2025). Growth of Pontianak Siamese Shoot Culture (Citrus nobilis var. microcarpa) Supplemented with Tomato Extract and BAP (6-benzylaminopurine). Jurnal Biologi Tropis, 25(4a), 513–521. https://doi.org/10.29303/jbt.v25i4a.9666

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Pontianak Siamese (Citrus nobilis var. microcarpa) is a superior agricultural commodity especially in Sambas Regency, West Borneo. One solution to assist in the supply of Pontianak Siamese seedlings is propagation with tissue culture. This research aims to determine the impact of supplementation of tomato extract and BAP on the growth of Pontianak Siamese shoot cultures. This research was conducted from February to May 2023, at Biology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University. The research design used a Completely Randomized factorial design with two factors. The first factor is the treatment of BAP with concentrations 0M; 10-7M; 10-6M; and 5.10-6 M, while the second factor was tomato extract with concentrations 0% 7.5%, 10%, and 12.5%. The research results show the treatment B3T0 (5.10-6 M BAP and 0% T) has the most leaves (7 strands), the longest root length (0.30 cm), and the most roots (1 root).

References

Abidin, Z. (1993). Dasar-Dasar Pengetahuan Tentang Zat Pengatur Tumbuh. Angkasa. Bandung.

Angkasa, S., & Fendy. (1993). Keprok Grabag yang Langka dari Merbabu. Trubus, 24(288): 74.

Anugrah, A.R. (2022). Proliferasi Tunas Anggrek Hitam (Coelogyne pandurata L.) dengan Pemberian BAP dan Ekstrak Tomat Secara In Vitro. Skripsi. Universitas Muhammadiyah Sumatera Utara. Medan.

Chika, S., Kurniawati, F., & Rahmani, T.P.D. (2021). Kajian budidaya tanaman anggrek Dendrobium sp. menggunakan teknik kultur meristem serta pengaruh penambahan berbagai ekstrak terhadap pertumbuhannya. In Prosiding Seminar Nasional Biologi, 7(1): 434-441. https://doi.org/10.24252/psb.v7i1.24915

Corina, I.P., Mukarlina, & Riza, L. (2014). Respon Pertumbuhan Kultur Biji Jeruk Siam Seed (Citrus nobilis var. microcarpa) dengan Penambahan Ekstrak Tauge dan Benzilaminopurine (BAP). Protobiont, 3(2): 120-1. https://doi.org/10.26418/protobiont.v3i2.5519

Dewi, L.L., Nurcahyani, E., Zulkifli, & Lande, M.L. (2021). Efek pemberian ekstrak tomat (Solanum lycopersicum L.) terhadap kandungan karbohidrat dan pertumbuhan planlet anggrek Dendrobium striaenopsis. Agritrop: Jurnal Ilmu-Ilmu Pertanian, 19(1): 67–73. https://doi.org/10.32528/agritrop.v19i1.5473

George, E.F., & Sherrington, P.O. (1984). Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories. Exegetics Limited. London

George, E.F., Hall, M.A., & Klerk, G.J.D. (2008). Plant Propagation by Tissue Culture 3rd Edition. The Background, (1): 1–28. https://doi.org/10.1007/978-1-4020-5005-3

Hardiansyah, B. (2022). Hormon dan Zat Pengatur Tumbuh (ZPT). National Taiwan University (NTU). Taiwan.

Hernita. (2004). Teknologi Perbanyakan Vegetatif Tanaman Buah. Jurnal Balai Pengkajian Teknologi Pertanian Jambi, 3(7): 61-84.

Isnaeni, E., & Habibah, N.A. (2014). Efektivitas Skarifikasi dan Suhu Perendaman terhadap Perkecambahan Biji Kepel (Stelechocarpus burahol Blume) Hook. F dan Thompson) secara In Vitro dan Ex Vitro. Jurnal MIPA, 37(2): 105-114. http://journal.unnes.ac.id/nju/index.php/JM

Mariamah, Mukarlina, & Linda, R. (2017). Pertumbuhan Kalus Tanaman Markisa (Passiflora sp.) dengan Penambahan Naphtalene Acetic Acid (NAA) dan 6-Benzyl Amino Purine (BAP). Protobiont, 6(3): 37-41. https://doi.org/10.26418/protobiont.v6i2.20801

Mokoginta, R.V., Simbala, H.E.I., & Mansauda, K.L.R. (2020). Uji Aktivitas Antioksidan Ekstrak Etanol Bulbus Bawang Dayak (Eleutherine americana Merr) Dengan Metode DPPH (1,1-Diphenyl-2-Picrylhydrazyl). PHARMACON, 9(3). https://doi.org/10.35799/pha.9.2020.30031

Mokoginta, B., Beatrix, D., & Doortje, M.F.S. (2021). Penggunaan Zat Pengatur Tumbuh Sitokinin Dan Ekstrak Bahan Organik Terhadap Pertumbuhan Anggrek Dendrobium Secara In-Vitro. Jurnal Cocos, 3: 1-12. https://doi.org/10.35791/cocos.v2i2.32446

Nurjanah, E. (2009). Pengaruh Kombinasi NaCl dan ZPT IBA pada Media MS Terhadap Pertumbuhan Galur Mutan Padi Secara In Vitro. Prodi Biologi. Fakultas Islam Negeri Syarif Hidayatullah. Tanggerang.

Pierik. (1987). In Vitro Culture of Higher Plants. Martinus Nijhoff Publisher. Netherland.

Purnomosidhi, P., Suparman J.M, & Roshetko, M. (2002). Perbanyakan dan budidaya tanaman buah-buahan dengan penekanan pada durian, mangga, jeruk, melinjo dan sawo. Pedoman Lapang, International Centre for Research in Agroforestry (ICRAF) dan Winrock International. Bogor.

Rita, S., Mukarlina, & Linda, R. (2017). Respon Pertumbuhan Tunas Lidah Buaya (Aloe barbadensis Mill.) dengan Penambahan Ekstrak Taoge dan BAP (Benzyl Amino Purine). Protobiont, 6(3): 142-146. https://doi.org/10.26418/protobiont.v6i3.22466

Rodziah, K., Ahmad, L.L., Rokiah, Z., & Hafsah, J. (2010). Basal Media for In Vitro Germination of Red-Purple Dragon Fruit (Hylocereus polyrhizus). Agrobiotech, 1(1): 88-93. https://journal.unisza.edu.my/agrobiotechnology/index.php/agrobiotechnology/article/view/9/8

Septasari, M., & Mercuriani, I.S. (2023). Inducing an axillary bud of Dendrobium Red Emperor ‘Prince’ with an addition of BAP in vitro. Indonesian Journal of Bioscience (IJOBI), 1(2): 74-84. https://doi.org/10.21831/ijobi.v1i2.216

Serliana, Mukarlina, & Linda, R. (2017). Pertumbuhan Anggrek Hitam (Coelogyne pandurata Lindl.) secara In Vitro Dengan Penambahan Ekstrak Tomat (Solanum lycopersicum L.) Dan Benzyl Amino Purine (BAP). Protobiont, 6(3): 310 – 315. https://doi.org/10.26418/protobiont.v6i3.22500

Setiawati, T., Nurzaman, M., Rosmiati E.S., & Pitaloka, G.G. (2016). Pertumbuhan tunas anggrek Dendrobium sp. menggunakan kombinasi benzyl amino purin (BAP) dengan ekstrak bahan organik pada media Vacin and Went (VW). Jurnal Pro-Life, 3(3): 143-152. doi:10.33541/pro-life.v3i3.93

Wattimena, G.A. (1992). Bioteknologi Tanaman, Departemen Pendidikan dan Kebudayaan, Dikti, Pusat Antar Universitas Bioteknologi. Institut Pertanian Bogor, Bogor.

Yuniastuti, E., Praswanto, & Harminingsih, I. (2010). Pengaruh Konsentrasi BAP Terhadap Multipikasi Tunas Anthurium (Anthurium andraeanum Linden.) pada Beberapa Media Dasar Secara In vitro. J. Caraka Tani, 25(1): 2-7. http://dx.doi.org/10.20961/carakatani.v25i1.15476

Zulkarnain. (2009). Kultur Jaringan Tanaman. Bumi Aksara. Jakarta.

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

Growth of Pontianak Siamese Shoot Culture (Citrus nobilis var. microcarpa) Supplemented with Tomato Extract and BAP (6-benzylaminopurine)

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow