Evaluation of Compatibility and Effectiveness of Fungal Lipase Crude Extract from Avocado as an Eco-Friendly Biodetergent

Prapti Sedijani; Kusmiyati Kusmiyati; AA Sukarso; Respi Eka Muhidayani

doi:10.29303/jbt.v25i4.10578

Evaluation of Compatibility and Effectiveness of Fungal Lipase Crude Extract from Avocado as an Eco-Friendly Biodetergent

Authors

Prapti Sedijani , Kusmiyati Kusmiyati , AA Sukarso , Respi Eka Muhidayani

DOI:

10.29303/jbt.v25i4.10578

Published:

2025-10-31

Issue:

Vol. 25 No. 4 (2025): Oktober-Desember

Keywords:

lipase fungi, crude extract, avocado, compatibility, effectivity, washing test

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Sedijani, P., Kusmiyati, K., Sukarso, A., & Muhidayani, R. E. (2025). Evaluation of Compatibility and Effectiveness of Fungal Lipase Crude Extract from Avocado as an Eco-Friendly Biodetergent. Jurnal Biologi Tropis, 25(4), 6082–6087. https://doi.org/10.29303/jbt.v25i4.10578

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Abstract

Chemical detergents may have negative impacts on both the environment and human health. The addition of enzymes to detergents can help reduce these effects. Therefore, discovering new enzyme sources that are compatible with commercial detergents and can enhance their cleaning performance may provide an ideal solution to this issue. This study aimed to evaluate the compatibility and effectiveness of a crude fungal lipase extract isolated from avocado flesh (AIF) when combined with commercial detergents, as well as to assess its potential in reducing the required amount of chemical detergent. Compatibility tests were conducted by comparing the washing performance of three commercial detergent brands, while effectiveness was evaluated based on washing performance under various detergent concentrations. The results showed that the crude fungal lipase extract was compatible with all tested detergents and had the potential to reduce chemical detergent usage by up to 50%.

References

Aehle, W. (2007). Enzymes in Industry: Production and Applications. Wiley-VCH.

Gupta, R., Beg, Q. K., & Lorenz, P. (2002). Bacterial alkaline proteases: molecular approaches and industrial applications. Applied Microbiology and Biotechnology, 59(1), 15–32.

Jaeger, K.-E., & Eggert, T. (2002). Lipases for biotechnology. Current Opinion in Biotechnology, 13(4), 390–397.

Kirk, O., Borchert, T. V., & Fuglsang, C. C. (2002). Industrial enzyme applications. Current Opinion in Biotechnology, 13(4), 345–351.

Kuo, C. H., & Haynes, R. J. (2011). The effects of pH, exchangeable cations and anions on enzyme activity in soils. Journal of Environmental Quality, 40(4), 1216–1225

Maurer, K. H. (2004). Detergent proteases. Current Opinion in Biotechnology, 15(4), 330–334.

Krajewska, B. (2018). Application of enzymes in detergents: Recent developments. Enzyme and Microbial Technology, 114, 1–17.

Banerjee, G., Ray, A., & Kumar, M. (2021). Microbial enzymes in detergent formulation: Recent developments and future perspectives. Journal of Cleaner Production, 285, 124858. https://doi.org/10.1016/j.jclepro.2020.124858

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254.

Hasan, F., Shah, A. A., & Hameed, A. (2020). Industrial applications of microbial lipases. Enzyme and Microbial Technology, 142, 109688. https://doi.org/10.1016/j.enzmictec.2020.109688

Jain, R., & Rathore, N. (2022). Food-grade microbes as sustainable enzyme producers: A review. Biotechnology Advances, 58, 107906. https://doi.org/10.1016/j.biotechadv.2022.107906

Krajewska, B. (2018). Application of enzymes in detergents: Recent developments. Enzyme and Microbial Technology, 114, 1–17.

Maurer, K. H. (2004). Detergent proteases. Current Opinion in Biotechnology, 15(4), 330–334. (sudah kamu masukkan, bisa diacu ulang di sini)

Mousavi, SA dan Khodadoost F (2019). Effects of detergents on natural ecosystems and wastewater treatment processes: a review. Epub. 26 (26): 26439-26448.

Nguyen, H. T., Kim, J. S., & Lee, D. H. (2022). Enhancement of detergent efficiency using natural lipase: A step toward sustainable cleaning technology. Applied Microbiology and Biotechnology, 106(14), 5411–5423. https://doi.org/10.1007/s00253-022-12003-y

Patel, P. S., Dutta, S., & Mondal, K. C. (2023). Synergistic action of lipase and protease in detergent applications: A review. International Journal of Biological Macromolecules, 243, 125132. https://doi.org/10.1016/j.ijbiomac.2023.125132

Sharma, R., Chisti, Y., & Banerjee, U. C. (2001). Production, purification, characterization, and applications of lipases. Biotechnology Advances, 19(8), 627–662.

Sedijani, P., Rasmi, D.A.C., Kusmiyati, K., & Anggriani, R.A. (2021). Powerful lipolytic Activity of Fungi Isolated from Coconut and Avocado Flesh on different pH and Temperature. Jurnal Penelitian Pendidikan IPA, 7(SpecialIssue), 365–369. ttps://doi.org/10.29303/jppipa.v7iSpecialIssue.1261

Sedijani , P; Khofia, N; Rasmi, DAC and Kusmiyati (2023). Fungal Crude Lipase Enzyme Produced Using the SSF (Solid State Fermentation) Method Increases The Washing Test Performance. Jurnal Biologi Tropis, 23 (1): 140 – 147. DOI: http://dx.doi.org/10.29303/jbt.v23i1.6046

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Evaluation of Compatibility and Effectiveness of Fungal Lipase Crude Extract from Avocado as an Eco-Friendly Biodetergent

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