Ethanol Extract Concentrations of Anredera cordifolia, Aloe vera and Centella asiatica L. Leaves as Staphylococcus aureus Growth Inhibitor
DOI:
10.29303/jbt.v25i4a.10636Published:
2025-11-21Downloads
Abstract
Excessive oil production, irritation, and bacterial infections, especially those produced by Staphylococcus aureus, are the main causes of acne, a common skin condition. The antibacterial qualities of a variety of therapeutic plants have long been utilized. This study aimed to evaluate the antibacterial effectiveness of ethanol extracts of Binahong (Anredera cordifolia), Aloe vera (Aloe vera), and Pegagan (Centella asiatica L.) leaves against the growth of Staphylococcus aureus. This research was a true experimental study using the Kirby-Bauer disk diffusion method. The ethanol concentrations used for extraction were 30%, 40%, and 50%, with meropenem as a comparison. The inhibition zones were measured after 24 hours of incubation at 37°C. The results showed that all extracts exhibited moderate antibacterial activity, with inhibition zones increasing along with higher extract concentrations. The highest mean inhibition zone was observed in Pegagan (8.9 mm), followed by Binahong (8.783 mm) and Aloe vera (8.26 mm). Statistical analysis showed that concentration (p = 0.005), day (p = 0.038), interaction between plant*day (p = 0.011), and plant*concentration*day (p = 0.001) have a significant effect on the inhibition zone diameter (p < 0.05). Meanwhile, plant (p = 0.244), interaction between plant*concentration (p = 0.380), and interaction between concentration*day (p = 0.342) show no significant differences in the inhibition zone diameter (p > 0.05). The conclusion is that there are differences in the inhibitory power of binahong, aloe vera, and pegagan plant extracts against Staphylococcus aureus bacteria used with 70% ethanol solvent.
Keywords:
Anredera cordifolia Aloe vera Antibacterial activity Centella asiatica Ethanol extract Staphylococcus aureusReferences
Ardini, D., & Rahayu, P. (2019). Studi Variasi Gelling Agent PVA (Propil Vinil Alkohol) pada Formulasi Masker Peel-off Ekstrak Lidah Buaya (Aloe vera) sebagai Anti Jerawat. Jurnal Kesehatan, 10(2), 245–251. https://doi.org/10.26630/jk.v10i2.1422
Ata, A., Ulfa, M., Emelda, E., Munir, M. A., & Sulistyani, N. (2023). Pengaruh Metode Ekstraksi Maserasi dan Sokletasi terhadap Standardisasi Parameter Spesifik dan Non Spesifik Ekstrak Etanol Biji Pepaya (Carica papaya L.). Jurnal Insan Farmasi Indonesia, 6(1), 1–12. https://doi.org/10.36387/jifi.v6i1.1387
Awaluddin, N., Farid, N., & Bachri, N. (2020). Uji Efektivitas Gel Ekstrak Etanol Daun Binahong (Anredera cordifolia) sebagai Penyembuhan Luka Insisi pada Tikus Wistar Jantan. Jurnal Kesehatan, 13(2). 10.24252/kesehatan.v13i2.16435
Dadiono, M. S., & Andayani, S. (2022). Potensi Tanaman Binahong (Anredera cordifolia) sebagai Obat Alternatif pada Bidang Akuakultur. Jurnal Perikanan Pantura (JPP), 5(1), 156. https://doi.org/10.30587/jpp.v5i1.3769
Davis, W. W., & Stout, T. R. (1971). Disc Plate Method of Microbiological Antibiotic Assay. Applied Microbiology, 22(4), 659–665. doi.org/10.1128/am.22.4.659-665.1971
Dewi, N. P., Vani, A. T., Abdullah, D., & Oktora, M. (2022). Uji Kuersetin pada Ekstrak Etanol Lidah Buaya (Aloe vera) dengan Metode Kromatografi Lapis Tipis. Nusantara Hasana Journal, 1(11), 59–69. https://repository.unbrah.ac.id/id/eprint/144
Fatimah, S., Prasetyaningsih, Y., & Astuti, R. W. (2022). Efektifitas Antibakteri Ekstrak Daun Pegagan (Centella asiatica) terhadap Pertumbuhan Bakteri Staphylococcus aureus. Jurnal Ilmu Kefarmasian, 3(1), 61. https://doi.org/10.31764/lf.v3i1.7233
Fitri, N., Nurlaeli, L., & Ely, A. F. (2024). Formulasi dan Evaluasi Fisik Sediaan Facial Wash dari Ekstrak Daun Pegagan (Centella asiatica L.) sebagai Bahan Aktif Antibakteri Staphylococcus aureus. Medical Research and Public Health Information Journal, 2(1). https://journal.myrepublikcorp.com/index.php/MERAPI/article/view/134
Imasari, T., & Emasari, F. (2022). Deteksi Bakteri Staphylococcus sp. Penyebab Jerawat dengan Tingkat Pengetahuan Perawatan Wajah pada Siswa Kelas XI di SMK Negeri 1 Pagerwojo. Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya, 2(2), 58–65. https://doi.org/10.56399/jst.v2i2.20
Intan, K., Diani, A., & Nurul, A. S. R. (2021). Aktivitas Antibakteri Kayu Manis (Cinnamomum burmanii) terhadap Pertumbuhan Staphylococcus aureus. Jurnal Kesehatan Perintis (Perintis’s Health Journal), 8(2), 121–127. https://doi.org/10.33653/jkp.v8i2.679
Khairunnisa, S., Hakim, A. R., & Audina, M. (2022). Perbandingan Kadar Flavonoid Total berdasarkan Perbedaan Konsentrasi Pelarut Etanol dari Ekstrak Daun Pegagan (Centella asiatica [L.]). Journal Pharmaceutical Care and Sciences, 3(1), 121–131. https://doi.org/10.33859/jpcs.v3i1.236
Lutfiah, A., Putri, M. W., & Mohammad, M. T. (2023). Uji Efektivitas Ekstrak Lidah Buaya (Aloe vera) dalam Menghambat Pertumbuhan Bakteri Propionibacterium acnes secara In Vitro. Jurnal Ilmiah Manusia dan Kesehatan, 6(2). https://doi.org/10.31850/makes.v6i2.2175
Marhaeni, I. L. S. (2020). Potensi Lidah Buaya (Aloe vera Linn) Sebagai Obat dan Sumber Pangan. Jurnal Pertanian Universitas Borobudur. https://ejournal.borobudur.ac.id/index.php/3/article/view/746
Nasution, J., Ramadhani, C. S., & Marianti, M. (2024). Studi Literatur Potensi Binahong (Anredera cordifolia) dalam Pengobatan Luka. Journal of Natural Sciences, 5(2), 133–142. https://doi.org/10.34007/jonas.v5i2.468
Nurhaida, D., Busnetty, I., Tambunan, T. T. H., & Munawar, M. A. (2023). Pemberdayaan Kelompok Ibu PKK Melalui Pelatihan Olahan Pangan Lidah Buaya dengan Metode Participatory Rural Appraisal. Buletin Udayana Mengabdi, 22(4), 235. https://doi.org/10.24843/BUM.2023.v22.i04.p11
Nurasmi, K. 2020. Uji Aktivitas Daun Binahong (Anredera Cordifolia (Ten.) Steenis) terhadap Cacing Ascaridia Galli secara In Vitro. Program Studi Kedokteran Hewan Fakultas Kedokteran Universitas Hasanuddin Makassar.
Purba, S. K. R. (2020). Uji Daya Hambat Ekstrak Jahe Merah (Zingiber officinale var. rubrum) terhadap Pertumbuhan Bakteri Staphylococcus aureus. The Indonesian Journal of Medical Laboratory, 1(1). https://ijml.jurnalsenior.com/index.php/ijml/article/view/11
Ratu, S. D. R., Fifendy, M., & Advinda, L. (2022). Pengaruh Berbagai Konsentrasi Sabun Cair Anti-Acne terhadap Staphylococcus aureus Bakteri Penyebab Jerawat. Jurnal Serambi Biologi, 7(4), 311–317. doi.org/10.53864/jifakfar.v6i1.122
Ritonga, L. S., Meutia, R., & Mutia, M. S. (2024). Formulasi dan Aktivitas Gel Ekstrak Daun Pegagan (Centella asiatica L. Urban) terhadap Pertumbuhan Bakteri Penyebab Jerawat. Jurnal Kesehatan Masyarakat, 8(2). 10.31004/prepotif.v8i2.30520
Sasebohe, V. Y., Prakasita, V. C., & Aditiyarini, D. (2023). Aktivitas Antibakteri Ekstrak Etanol Daun Binahong terhadap Staphylococcus aureus dan Propionibacterium acnes Penyebab Jerawat. Science Scitatio, 4(1), 41–107. https://doi.org/10.21460/sciscitatio.v4i1.41
Sulistio, A. D. (2020). Pemanfaatan Daun Pegagan (Centella asiatica) menjadi Olahan Keripik oleh Masyarakat Desa Wisata Jatimulyo, Girimulyo. Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA, 5(2), 125–130. https://jurnal.uny.ac.id/index.php/jpmmp/article/view/44317
Tarigan, R. V. B., & Sambahtra, D. (2022). Uji Antifertilitas Ekstrak Lidah Buaya (Aloe vera L.) terhadap Kuantitas Spermatozoa dan Ekspresi Cyclooxygenase-2 pada Testis Mencit (Mus musculus L.). Journal of Indonesian Medical Laboratory and Science, 3(1), 51–64. https://jurnal.aiptlmi-iasmlt.id/index.php/joimedlabs/article/view/65
Trisnawita, Y., Mahyani, N., & Puspita, M. (2024). Potensi Antibakteri Sediaan Gel Facial Wash Ekstrak Etanol Daun Binahong (Anredera cordifolia) terhadap Bakteri Propionibacterium acnes. Jurnal Sains dan Teknologi, 1(1).
Tumundo, C. S., & Wewengkang, D. S. (2024). Uji Potensi Antibakteri Ekstrak Spons Stylissa carteri dari Perairan Poopoh Minahasa terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa. Jurnal Pharmacon, 13(1), 529–539. https://doi.org/10.35799/pha.13.2024.49697
Widiastuti, D., Karima, I. F., & Setiyani, E. (2019). Efek Antibakteri Sodium Hypochlorite terhadap Staphylococcus aureus. Jurnal Ilmiah Kesehatan Masyarakat, 11(4). Dikutip pada 18 Maret 2025 dari https://jikm.upnvj.ac.id/index.php/home/article/view/34
Wijaya, H., Jubaidah, S., & Rukayyah. (2022). Perbandingan Metode Ekstraksi Maserasi dan Sokhletasi terhadap Rendemen Ekstrak Batang Turi (Sesbania grandiflora L.). Indonesian Journal of Pharmacy and Natural Product, 5(1). Dikutip pada 20 Maret 2025 dari DOI: 10.35473/ijpnp.v5i1.1469
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