Isolation and Antibacterial Activity Test of Mangrove Rhizosphere Bacteria Against Escherichia coli and Staphylococcus aureus

Okto Asriatno; Dewinta Nur Alvionita; Muhammad Syarief; Dwirariska Aprilianasari Syamsuardi

doi:10.29303/jbt.v25i4b.10875

Isolation and Antibacterial Activity Test of Mangrove Rhizosphere Bacteria Against Escherichia coli and Staphylococcus aureus

Authors

Okto Asriatno , Dewinta Nur Alvionita , Muhammad Syarief , Dwirariska Aprilianasari Syamsuardi

DOI:

10.29303/jbt.v25i4b.10875

Published:

2025-12-16

Issue:

Vol. 25 No. 4b (2025): Special Issue

Keywords:

Antibacterial activity, Escherichia coli, Isolation, Mangrove Rhiozosphere, Staphylococcus aureus

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Asriatno, O., Dewinta Nur Alvionita, Muhammad Syarief, & Dwirariska Aprilianasari Syamsuardi. (2025). Isolation and Antibacterial Activity Test of Mangrove Rhizosphere Bacteria Against Escherichia coli and Staphylococcus aureus. Jurnal Biologi Tropis, 25(4b), 421–428. https://doi.org/10.29303/jbt.v25i4b.10875

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Abstract

The global rise in multidrug-resistant pathogens necessitates the exploration of unique ecological niches, such as the mangrove rhizosphere, as promising reservoirs for novel antibacterial agents. In this study, we aimed to isolate and characterize bacteria from the rhizosphere of Rhizophora sp. in the mangrove forest of Bypass Kendari, Southeast Sulawesi, and to evaluate their antibacterial activity against Escherichia coli and Staphylococcus aureus. Isolation was performed using the spread plate method on three different media: Nutrient Agar (NA), Zobell Marine Agar (ZMA), and International Streptomyces Project 2 (ISP2). A total of seven bacterial isolates were obtained (NA.1, ZMA.1, ZMA.2, ZMA.x, ISP2.1, ISP2.2, and ISP2.x), exhibiting diverse morphological characteristics ranging from mucoid to smooth colonies. Antibacterial screening using a modified Kirby-Bauer disc diffusion method revealed that only one isolate, ZMA.1, possessed broad-spectrum antagonistic activity, effectively inhibiting both the Escherichia coli and Staphylococcus aureus. Further microscopic identification via Gram staining characterized isolate ZMA.1 as a Gram-negative and rod-shaped bacteria, appearing in monobacillus and diplobacillus arrangements. These findings underscore the potential of Gram-negative bacteria from the mangrove rhizosphere as a promising source of new antibacterial candidates to combat multidrug-resistant pathogens.

References

Alsamannoudi, H., Alamri, A., Almotiri, T., Alharbi, S., Alshammari, D., Alnasser, B., & Alswayeh, H. (2021). Antimicrobial resistance: A growing global threat. International Journal of Health Sciences, 3(S1): 145–151. https://doi.org/10.53730/ijhs.v3nS1.15079

Ambeng, Zubair, H., Oka, N. P., & Tonggiroh, A. (2019). Potential of soil bacteria isolated from mangrove rhizosphere in Sinjai Regency to suppress the growth of Vibrio harveyi. Journal of Physics: Conference Series, 1341(2), Article 022016. https://doi.org/10.1088/1742-6596/1341/2/022016

Azzahra, F., Prasetyawati, E. T., & Lestari, S. R. (2025). Isolation and characterization of endophytic bacteria from the roots of Avicennia sp. in the mangrove area of Gunung Anyar, Surabaya. Jurnal Pembelajaran dan Biologi Nukleus, 11(1), 219–232. https://doi.org/10.36987/jpbn.v11i1.6596

Birkelbach, J., Seyfert, C. E., Walesch, S., & Muller, R. (2024). Harnessing Gram-negative bacteria for novel anti-Gram-negative antibiotics. Microbial Biotechnology, 17(1), 1–10. https://doi.org/10.1111/1751-7915.70032

Breijyeh, Z., Jubeh, B., & Karaman, R. (2020). Resistance of Gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules, 25(6), Article 1340. https://doi.org/10.3390/molecules25061340

Chau, K. M., Quyen, D. V., Fraser, J. M., Smith, A. T., Van, T. T. H., & Moore, R. J. (2020). Broad spectrum antimicrobial activities from spore-forming bacteria isolated from the Vietnam Sea. PeerJ, 8, Article e10117. https://doi.org/10.7717/peerj.10117

Darabpour, E., Ardakani, M. R., Motamedi, H., & Ronagh, M. T. (2011). Isolation of a broad spectrum antibiotic producer bacterium, Pseudoalteromonas piscicida PG-02, from the Persian Gulf. Bangladesh Journal of Pharmacology, 6(2), 74–83. https://doi.org/10.3329/bjp.v6i2.8592

Fatimah, Rahayuningtyas, N. D., Nastiti, A., Alawiyah, D. D., Ramadhan, R., Geraldi, A., & Junairiah. (2024). Antibacterial and biosurfactant activity of endophytic bacteria isolated from mangrove plant in Lamongan, Indonesia. Biodiversitas, 25(7), 3035–3042. https://doi.org/10.13057/biodiv/d250725

Gopalakhrishnan, S., Sunder, J., Sasidharan, V., & Subramanian, S. E. (2016). Antibacterial activity of actinobacteria isolated from mangroves of Andaman and Nicobar Islands, India. Advances in Animal and Veterinary Sciences, 4(5), 230–236. https://doi.org/10.14737/journal.aavs/2016/4.5.230.236

Gupta, P., & Diwan, B. (2017). Bacterial exopolysaccharide mediated heavy metal removal: A review on biosynthesis, mechanism, and remediation strategies. Biotechnology Reports, 13, 58–71. https://doi.org/10.1016/j.btre.2016.12.006

He, W., Huan, X., Li, Y., Deng, Q., Chen, T., Xiao, W., Chen, Y., Ma, L., Liu, N., Shang, Z., & Wang, Z. (2025). A broad-spectrum antibiotic targets multiple-drug-resistant bacteria with dual binding targets and no detectable resistance. Nature Communications, 16, Article 7048. https://doi.org/10.1038/s41467-025-62407-4

Hutchings, M. I., Truman, A. W., & Wilkinson, B. (2019). Antibiotics: Past, present and future. Current Opinion in Microbiology, 51, 72–80. https://doi.org/10.1016/j.mib.2019.10.008

Kapoor, G., Saigal, S., & Elongavan, A. (2017). Action and resistance mechanisms of antibiotics: A guide for clinicians. Journal of Anaesthesiology Clinical Pharmacology, 33(3), 300–305. https://doi.org/10.4103/joacp.JOACP_349_15

Khairillah, Y. N., Alang, H., Haryanto, A., Syamsia, & Rismawati. (2024). Isolation and identification of bacteria producing antibiotic compounds from the rhizosphere of Avicennia marina against pathogenic bacteria in the mangrove ecosystem area of West Kalimantan. Bioscientist: Jurnal Ilmiah Biologi, 12(2), 1679–1691. https://doi.org/10.33394/bioscientist.v12i2.12527

Munita, J. M., & Arias, C. A. (2016). Mechanisms of antibiotic resistance. Microbiology Spectrum, 4(2). https://doi.org/10.1128/microbiolspec.VMBF-0016-2015

Murray, C. J. L., Ikuta, K. S., Sharara, F., et al. (2022). Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis. The Lancet, 399(10325), 629–655. https://doi.org/10.1016/S0140-6736(21)02724-0

Muwawa, E. M., Makonde, H. M., Obieze, C. C., de Oliveira, I. G., Jefwa, J. M., Kahindi, J. H. P., & Khasa, D. P. (2024). Diversity and assembly patterns of mangrove rhizosphere mycobiome along the coast of Gazi Bay and Mida Creek in Kenya. PLoS ONE, 19(4), Article e0298237. https://doi.org/10.1371/journal.pone.0298237

Palit, K., Rath, S., Chatterjee, S., & Das, S. (2022). Microbial diversity and ecological interactions of microorganisms in the mangrove ecosystem: Threats, vulnerability, and adaptations. Environmental Science and Pollution Research, 29, 32467–32512. https://doi.org/10.1007/s11356-022-19048-7

Pramono, H., Irawan, N. T., Firdaus, M. R. A., Sudarno, Sulmartiwi, L., & Mubarak, A. S. (2019). Bacterial endophytes from mangrove leaves with antibacterial and enzymatic activities. Malaysian Journal of Microbiology, 15(7), 543–553. https://doi.org/10.21161/mjm.190352

Sari, R., Apridamayanti, P., & Pratiwi, L. (2022). Efektivitas SNEDDS kombinasi fraksi etil asetat daun cengkodok (Melastoma malabathricum) - antibiotik terhadap bakteri hasil isolat dari pasien ulkus diabetik. Pharmaceutical Journal of Indonesia, 7(2), 105–114. https://doi.org/10.21776/ub.pji.2022.007.02.5

Shanthakumar, S. P., Duraisamy, P., Vishwanath, G., Selvanesan, B. C., Ramaraj, V., & David, B. V. (2015). Broad spectrum antimicrobial compounds from the bacterium Exiguobacterium mexicanum MSSRFS9. Microbiological Research, 178, 59–65. https://doi.org/10.1016/j.micres.2015.06.007

Sharma, D., Kaur, T., Chadha, B. S., & Manhas, R. K. (2011). Antimicrobial activity of actinomycetes against multidrug resistant Staphylococcus aureus, Escherichia coli and various other pathogens. Tropical Journal of Pharmaceutical Research, 10(6), 801–808. https://doi.org/10.4314/tjpr.v10i6.14

Tumundo, C., Wewengkang, D. S., & Jumriadi. (2024). Uji potensi antibakteri ekstrak spons Stylissa carteri dari perairan Poopoh Minahasa terhadap bakteri Staphylococcus aureus dan Pseudomonas aeruginosa. PHARMACON, 13(1), 529–539. https://doi.org/10.35799/pha.13.2024.49697

Uddin, T. M., Chakraborty, A. J., Khusro, A., Zidan, B. M. R. M., Mitra, S., Emran, T., Dhama, K., Ripon, M. K. H., Gajdacs, M., Sahibzada, M. U. K., Hossain, M. J., & Koirala, N. (2021). Antibiotic resistance in microbes: History, mechanisms, therapeutic strategies and future prospects. Journal of Infection and Public Health, 14(12), 1750–1766. https://doi.org/10.1016/j.jiph.2021.10.020

Zhang, Y., Gui, H., Zhang, S., & Li, C. (2022). Diversity and potential function of prokaryotic and eukaryotic communities from different mangrove sediments. Sustainability, 14(6), Article 3333. https://doi.org/10.3390/su14063333

Zhongsheng, Y., Zhihao, Z., & Fang, L. (2023). Community structures of mangrove endophytic and rhizosphere bacteria in Zhangjiangkou National Mangrove Nature Reserve. Scientific Reports, 13, Article 17127. https://doi.org/10.1038/s41598-023-44447-2

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Isolation and Antibacterial Activity Test of Mangrove Rhizosphere Bacteria Against Escherichia coli and Staphylococcus aureus

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