Antioxidant Activity and Nanoemulsion Formulation of Chlorella vulgaris Ethanolic Extract for Potential Topical Applications
DOI:
10.29303/jbt.v26i3.11959Published:
2026-07-17Downloads
Abstract
Chlorella vulgaris is a marine microalga abundant in West Nusa Tenggara (NTB) that contains flavonoids and other bioactive compounds exhibiting potent antioxidant activity. Antioxidant compounds can be utilized in skin maintenance. This research investigated the antioxidant activity of C. vulgaris ethanolic extract while developing a nanoemulsion formulation intended for topical application. Biomass was processed into simplisia and extracted by maceration using ethanol 96%. Phytochemical identification and total flavonoid content were determined using TLC and aluminum chloride methods. Antioxidant activity was determined using the DPPH radical scavenging assay and expressed as IC₅₀ values. The extract was subsequently formulated into nanoemulsions using virgin coconut oil, Tween 80, and Span 80, followed by physical characterization. The extraction process produced a C. vulgaris extract yield of 7.14% (w/w). TLC analysis confirmed the presence of flavonoids, and the TFC of the extract was 89,31 ± 0,03 mg QE/g extract. Strong antioxidant activity was demonstrated by the ethanolic extract, with an IC₅₀ of 52.869 ppm. The nanoemulsion formulation containing 1% extract exhibited a green color, characteristic odor, clear appearance, with a pH value of 6,96, viscosity of 990 cPs, a transmittance value of 89,8%, particle size of 16,5 nm, and polydispersity index 0,294, indicating the formation of a nanosized dispersion with moderate particle size distribution. Overall, Nanoemulsion delivery enhances C. vulgaris antioxidant performance, supporting its potential application in skin maintenance and cosmeceutical products.
Keywords:
Antioxidant activity Chlorella vulgaris Nanoemulsion Total flavonoid content Topical formulationReferences
Alhasso, B., Ghori, M. U., & Conway, B. R. (2023). Development of nanoemulsions for topical application of mupirocin. Pharmaceutics, 15(2), Article 378. https://doi.org/10.3390/pharmaceutics15020378
Baitariza, A., Darijanto, S. T., Pamudjie, J. S., Fidrianny, I., & Suherman, S. E. (2014). Formulasi dan Evaluasi Mikroemulsi Antikerut Ekstrak Beras Hitam (Oryza sativa L.). Indonesian Journal of Pharmaceutical Science and Technology, 1(1), 14-18.
Bhardwaj, S., Gaur, P. K., & Tiwari, A. (2022). Development of topical nanoemulgel using combined therapy for treating psoriasis. ASSAY and Drug Development Technologies, 20(1), 42–54. https://doi.org/10.1089/adt.2021.112
Chamidah, A., Afrilia, H. C., Ahmad, M. G., & Arisandi, D. (2024). Isolasi klorofil a dan analisis aktivitas antioksidan dari mikroalga Chlorella vulgaris. Jurnal Pengolahan Hasil Perikanan Indonesia, 27(11), 1006–1020. https://doi.org/10.17844/jphpi.v27i11.57470
Fan, X.; Bai, L.; Zhu, L.; Yang, L.; Zhang, X. (2014). Marine algae-derived bioactive peptides for human nutrition and health. J. Agric. Food Chem. 62, pp. 9211-9222. https://dx.doi.org/10.1021/jf502420h
Fassi Fihri, R., Ez-Zoubi, A., Mbarkiou, L., Amar, A., Farah, A., & Bouchamma, E. O. (2024). Antibacterial and antioxidant activities of Chlorella vulgaris using natural deep eutectic solvent. Heliyon, 10(15), e35071. https://doi.org/10.1016/j.heliyon.2024.e35071
García-Segovia, P., Uribe-Wandurraga, Z. N., Igual, M., Mullor, J. L., & Martínez-Monzó, J. (2024). Lipid extract of microalgae Chlorella vulgaris as a bioactive ingredient. Journal of Food Science, 89(7), 4123–4135. https://doi.org/10.1111/1750-3841.17025
Georgiopoulou, I., Tzima, S., Louli, V., & Magoulas, K. (2023). Process optimization of microwave-assisted extraction of chlorophyll, carotenoid and phenolic compounds from Chlorella vulgaris and comparison with conventional and supercritical fluid extraction. Applied Sciences, 13(4), 2740. https://doi.org/10.3390/app13042740
Gharib, F. A., Osama, K., Abd El Sattar, A. M., & Ahmed, E. Z. (2024). Microalgae as bio-stimulants and antioxidant sources. Scientific Reports, 14, 1398. https://doi.org/10.1038/s41598-023-50040-4
Hamouda, R. A., Amera Abd, E. L., Elkaw, E. M., Alotaibi, A. S., Asma, M. A., & Hamza, H. A. (2022). Assessment of antioxidant and anticancer activities of microgreen alga Chlorella vulgaris and its blend with different vitamins. Molecules, 27(5), 1602. https://doi.org/10.3390/molecules27051602
Hernández, A. R., Sepúlveda, L., Hata, Y., Castellanos, L., Björklund, S., & Aragón, M. (2025). Algae extract-based nanoemulsions for photoprotection against UVB radiation. Scientific Reports, 15, 1911. https://doi.org/10.1038/s41598-025-85604-z
Hussein, H. J., Naji, S. S., & Al-Khafaji, N. (2018). Antibacterial properties of the Chlorella vulgaris isolated from polluted water in iraq. Journal of Pharmaceutical Sciences and Research, 10(10), 2457-2460.
Iriani, D., Feliatra, Hasan, B., Karnila, R., Chaiyanate, N., & Rozi. (2024). Biochemical processes and antioxidant properties of Chlorella vulgaris. Jurnal Ilmiah Perikanan dan Kelautan, 17(1), 53–67. https://doi.org/10.20473/jipk.v17i1.61083
Jaiswal, M., Dudhe, R., & Sharma, P. K. (2015). Nanoemulsion: An advanced mode of drug delivery system. 3 Biotech, 5(2), 123–127. https://doi.org/10.1007/s13205-014-0214-0
Kumar, M., Bishnoi, R. S., Shukla, A. K., & Jain, C. P. (2019). Techniques for formulation of nanoemulsion drug delivery system: a review. Preventive nutrition and food science, 24(3), 225. https://doi.org/10.3746/pnf.2019.24.3.225
Kumar, R., Kaur, K., Uppal, S., & Mehta, S. K. (2017). Ultrasound processed nanoemulsion: A comparative approach between resveratrol and resveratrol cyclodextrin inclusion complex to study its binding interactions, antioxidant activity and UV light stability. Ultrasonics sonochemistry, 37(1), 478-489. https://doi.org/10.1016/j.ultsonch.2017.02.024
Nastiti, C. M. R. R., & Riswanto, F. D. O. (2021). Analytical method validation and formula optimization of topical nanoemulsion formulation containing resveratrol. Indonesian Journal of Chemistry, 21(5), 1308–1318. https://doi.org/10.22146/ijc.63730
Ningsih, S. D., Henri, H., Roanisca, O., & Gus Mahardika, R. (2020). Skrining Fitokimia dan Penetapan Kandungan Total Fenolik Ekstrak Daun Tumbuhan Sapu-Sapu (Baeckea frutescens L.). Biotropika: Journal of Tropical Biology, 8(3), 178–185.https://doi.org/10.21776/ub.biotropika.2020.008.03.06
Nursal, F. K., Sumirtapura, Y. C., Suciati, T., & Kartasasmita, R. E. (2019). Optimasi Nanoemulsi Natrium Askorbil Fosfat melalui Pendekatan Design of Experiment (Metode Box Behnken). Jurnal Sains Farmasi & Klinis, 6(3), 228-236. https://doi.org/10.25077/jsfk.6.3.228-236.2019
Savvidou, M. G., Georgiopoulou, I., Antoniou, N., Tzima, S., Kontou, M., Louli, V., . . . Kolisis, F. N. (2023). Extracts from Chlorella vulgarisprotect mesenchymal stromal cells from oxidative stress induced by hydrogen peroxide. Plants, 12(2), 361. https://doi.org/10.3390/plants12020361
Shoviantari, F., Liziarmezilia, Z., Bahing, A., & Agustina, L. (2019). Uji aktivitas tonik rambut nanoemulsi minyak kemiri (Aleurites moluccana L.). Jurnal Farmasi dan Ilmu Kefarmasian Indonesia, 6(2), 69-73. https://doi.org/10.20473/jfiki.v6i22019.69-73
Shriram, Ravi Gundadka, Afrasim Moin, Hadil Faris Alotaibi, El-Sayed Khafagy, Ahmed Al Saqr, Amr Selim Abu Lila, and Rompicherla Narayana Charyulu. 2022. “Phytosomes as a Plausible Nano-Delivery System for Enhanced Oral Bioavailability and Improved Hepatoprotective Activity of Silymarin.” Pharmaceuticals (Basel, Switzerland) 15(7):790. https://doi.org/0.3390/ph15070790
Ye, H.; Wang, K.; Zhou, C.; Liu, J.; Zeng, X. (2008). Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chem. 111, pp. 428-432. https://dx.doi.org/10.1016/j.foodchem.2008.04.012
Yu, H., Ge, X., Huang, D., Xue, C., Ren, M., & Liang, H. (2023). Dietary supplementation of Chlorella vulgaris effectively enhanced the intestinal antioxidant capacity and immune status of micropterus salmoides. Antioxidants, 12(8), 1565. https://doi.org/10.3390/antiox12081565
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