Pemilihan Bagian Eksplan pada Stek Batang Tanaman Sambung Nyawa
Authors
Ratna Dewi Eskundari , Nur Rohimah HanikDOI:
10.29303/jbt.v20i2.1791Published:
2020-05-13Issue:
Vol. 20 No. 2 (2020): Mei - AgustusKeywords:
organogenesis, propagation, sambung nyawa, cuttings,Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
Abstrak: Tanaman sambung nyawa (Gynura procumbens) dikenal sebagai salah satu tanaman yang bermanfaat bagi kesehatan tubuh. Daun tanaman ini diketahui mempunyai efek anti peradangan yang dapat mengurangi bahkan mengobati berbagai macam penyakit. Penelitian ini berfokus pada pemilihan bagian batang tanaman sambung nyawa yang mempunyai kapasitas terbaik sebagai eksplan stek. Penelitian ini dilakukan dengan menanam 3 bagian batang tanaman sambung nyawa yang berbeda, yaitu bagian atas (pucuk sampai dengan 10 cm ke bawah), tengah (bagian batang yang berjarak 10cm dari pucuk sampai 20 cm), dan bawah (bagian batang yang berjarak 20 cm dari pucuk hingga 30 cm). Ketiga jenis eksplan ini ditanam di tanah yang mengandung humus tanpa diberi perlakuan penambahan ZPT. Penelitian ini menggunakan rancangan acak lengkap dengan sepuluh ulangan. Hasil penelitian menunjukkan bahwa tidak ada perbedaan yang nyata antara bagian batang dengan panjang daun, tetapi terdapat perbedaan yang nyata dengan lebar daun, panjang tunas, dan panjang akar. Hasil penelitian ini diharapkan dapat menjadi rujukan bagian batang ââ¬Åtengahââ¬Â dan atau ââ¬Åbawahââ¬Â sebagai eksplan terbaik dalam usaha perbanyakan tanaman sambung nyawa menggunakan stek.
Kata kunci: organogenesis, perbanyakan, sambung nyawa, stek.
Abstract: Sambung nyawa plant (Gynura procumbens) is known as one of the plants that are beneficial for human health. The leaves of this plant are known to have anti-inflammatory effects that can reduce and even treat various diseases. This research focused on the selection of the stem part of sambung nyawa plant which has the best capacity as explant cuttings. This research was carried out by planting three different parts of the stem of the plant, namely the upper part (shoots apical up to 10 cm down part), the middle (the stem part that is 10cm from the top to 20 cm down part), and the bottom (the part of the stem which is 20 cm apart from shoots up to 30 cm down part). These three types of explants were planted in soil containing humus without being treated with the addition of PGR(s). This study used a completely randomized design with ten replications. The results showed that there was no significant differences between the stem parts with leaf length parameter, but there were significant differences in leaf width, shoot length, and root length ones. The results of this study were expected to be a reference part of the stem "middle" and or "bottom" as the best explant in the effort to propagate the sambung nyawa plants using cuttings.
Keywords: organogenesis, propagation, sambung nyawa, cuttings.ÃÂ
References
Chandler, J.W., Cole, M., Flier, A., Grewe, B. & Werr, W. (2007). The AP2 Transcription Factors DORNROSCHEN and DORNRO SCHEN-LIKE Redundantly Control Arabidopsis Embryo Patterning via Interaction with PHAVOLUTA. Development, 134: 1653-1662. DOI: 10.1242/dev.001016.
Chatfield, S.P., Capron, R., Severino, A., Penttila, P.A., Alfred, S., Nahal, H. & Provart, N.J. (2013). Incipient Stem Cell Niche Conversion in Tissue Culture: Using a Systems Approach to Probe Early Events in WUSCHEL Dependent Conversion of Lateral Root Primordia into Shoot Meristems. Plant Journal, 73: 798ââ¬â813. DOI: 10.1111/tpj.12085.
Eskundari, R.D., Taryono, T., Indradewa, D. & Purwestri, Y.A. (2019). Protein Profile of Tissue Culture of TRI2025 Tea Clone. Biosaintifika: Journal of Biology & Biology Education, 11(1): 8-14. DOI:https://doi.org/10.15294/biosaintifika.v11i1.17522.
Gordon, S.P., Heisler, M.G., Reddy, G. V., Ohno, C., Das, P. & Meyerowitz, E.M. (2007). Pattern Formation during de novo Assembly of the Arabidopsis Shoot Meristem. Development, 134: 3539-3548. DOI: 10.1242/dev.010298.
Hoesen, D.S.H. (2001). Perbanyakan dan Penyimpanan Kultur Sambung Nyawa (Gynura procumbens (Lour.) Merr) dengan Teknik In-Vitro. Berita Biologi, 5(4): 379-385. DOI: 10.14203/beritabiologi.v5i4.1122.
Ikeuchi, M., Ogawa, Y., Iwase, A. & Sugimoto, K. (2016). ââ¬ÅPlant Regeneration: Cellular Origins and Molecular Mechanismsââ¬Â, Development, 143: 1442-1451. DOI: 10.1242/dev.134668.
Kirch, T., Simon, R., Grunewald, M. & Werr, W. (2003). The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 Gene of Arabidopsis Acts in the Control of Meristem Cell Fate and Lateral Organ Development. Plant Cell, 15: 694-705. DOI: 10.1105/tpc.009480
Liu, J., Sheng, L., Xu, Y., Li, J., Yang, Z., Huang, H. & Xu, L. (2014). WOX11 and 12 are Involved in the First-Step Cell Fate Transition during de novo Root Organogenesis in Arabidopsis, Plant Cell, 26: 1081-1093. DOI: https://doi.org/10.1105/tpc.114.122887.
Meiyanto, E. (1996). Efek Antimutagenik Beberapa Fraksi Ekstrak Alkohol Daun Gynura procumbens (Lour.) Merr. Laporan Penelitian, Fakultas Farmasi UGM, Yogyakarta.
Meiyanto, E., Susilowati, S., Tasminatun, S. & Murwanti, R., Sugiyanto. (2007). Efek Ekstrak Etanolik Gynura procumbens (Lour.) Merr. terhadap Penghambatan Pertumbuhan Tumor Payudara Tikus yang Diinduksi DMBA, Majalah Farmasi Indonesia, 18(4): 169-175.
Mokshina, N, Gorshkova, T. & Deyholos, M.K. (2014) Chitinase-Like (CTL) and Cellulose Synthase (CESA) Gene Expression in Gelatinous-Type Cellulosic Walls of Flax (Linum usitatissimum L.) Bast Fibers. PLoS ONE, 9(6): e97949. doi:10.1371/journal.pone.0097949
Oboho, E.G. & Iyadi, J.N. (2013). Rooting Potential of Mature Stem Cuttings of Some Forest Tree Species for Vegetative Propagation. Journal of Applied and Natural Science, 5 (2): 442-446. DOI: https://doi.org/10.31018/jans.v5i2.350
Omagbemi, O. B. (2003): The Rooting of Juvenile Shoot Cuttings of Some Timber and Fruit Trees. Unpublished dissertation submitted to the Department of Forestry and Wildlife, University of Benin, Benin City.
Putri, K. P. & Danu. (2014). Pengaruh Umur Bahan Stek dan Zat Pengatur Tumbuh terhadap Keberhasilan Stek Kemenyan (Styrax benzoin Dryand). Jurnal Penelitian Hutan Tanaman, 11(3): 141-147. DOI: https://doi.org/10.20886/jpht.2014.11.3.141-147
Ren, W., Qiao, Z., Wang, H., Zhu, L. & Zhang, L. (2003). Flavonoids: Promising Anticancer Agents, Medicinal Research Review, 23(4): 519-534. DOI: 10.1002/med.10033
Santoso, B.B., Hasnam, Hariyadi, Susanto, S. & Purwoko, B.S. (2008). Perbanyakan Vegetatif Tanaman Jarak Pagar (Jatropa curcas L.) dengan Stek Batang: Pengaruh Panjang dan Diameter Stek, Buletin Agronomi, 36 (3): 255-262. DOI: https://doi.org/10.24831/jai.v36i3.1385
Sudarsono, Gunawan, D., Wahyuono, S., Donatus, I.A. & Purnomo. (2002). Tumbuhan Obat II, Hasil Penelitian, Sifat-sifat dan Penggunaan, 96-100, Pusat Studi Obat Tradisional, Universitas Gadjah Mada, Yogyakarta.
Sugiyanto, Sudarto, B., Meiyanto, E., Nugroho, A.E. & Jenie, U.A. (2003). Aktivitas Antikarsinogenik Senyawa yang Berasal dari Tumbuhan, Majalah Farmasi Indonesia, 14 (4), 216-225.
Utami & Siregar, H. M. (1998). Upaya Meningkatkan Produktivitas Daun Dewa [Gynura procumbens (Lour.) Merr.] dengan Pemupukan. Laporan Teknik Proyek Penelitian, Pengembangan dan Pendayagunaan Biota Darat Tahun 1997/1998. Puslitbang Biologi-LIPI. Hlm. 384.
Wei, K., Wang, L.Y., Wu, L.Y., Zhang, C.C., Li, H.L., Tan, L.Q., Cao, H.L. & Cheng, H. (2014). Transcriptome Analysis of Indole-3-Butyric Acid-Induced Adventitious Root Formation in Nodal Cuttings of Camellia sinensis (L.). PLoS ONE, 9(9): e107201. DOI:10.1371/journal.pone.0107201.
License
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.