Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria

Muhammad Hasan Basri; Lalu Zulkifli; Abdul Syukur

doi:10.29303/jbt.v21i1.2340

Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria

Authors

Muhammad Hasan Basri , Lalu Zulkifli , Abdul Syukur

DOI:

10.29303/jbt.v21i1.2340

Published:

2021-01-20

Issue:

Vol. 21 No. 1 (2021): Januari - April

Keywords:

Vitex trifolia L, PIRG, Endophytic fungi, Sclerotium rolfsii.

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Hasan Basri, M., Zulkifli, L., & Syukur, A. (2021). Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria. Jurnal Biologi Tropis, 21(1), 72–80. https://doi.org/10.29303/jbt.v21i1.2340

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Abstract

Plant damage by pathogenic fungi is often found in plants, one of which is caused by Sclerotium rolfsii. Biological control strategy offers a promising alternative for managing disease in plants because they are environmental friendly compared to pesticides application. One of the biological control offered is by using endophytic fungi isolated from Vitex trivolia L. The aim of the study was to isolate, to identify macroscopic and microscopic endophytic fungi from Vitex trifolia L and to test their antagonism potency against the pathogenic fungus Sclerotium rolfsii in vitro. The isolation obtained 7 endophytic fungi isolates identified based on their genus characteristics, nsmely Periconia sp, Aspergillussp, DendrophomaÃÂ sp, GeotrichumÃÂ sp, AmpulliferinaÃÂ sp, ChalaraÃÂ sp, dan Bispora sp and 2 isolates have not been identified. The Antibacterial test of the fungi isolate on the 4 tested bacteria showed that of all the fungi isolate have low activity. The antagonism test using the direct opposition method with the PIRG formula, showed that the 3 isolates had high percentage of growth inhibition, in which ALJ1, BLJ5, and ALJ3 isolate has ÃÂ 85%, 90%, and 100% respectively. This potency could be used as biological agents on the pathogenic fungus Sclerotium rolfsii.

References

Hasiani, V. V., Ahmad, I., & Rijai, L. (2015). Isolasi jamur endofit dan produksi metabolit sekunder antioksidan dari daun pacar (Lawsonia inermis L.). Jurnal Sains dan Kesehatan, 1(4), 146-153. https://jsk.farmasi.unmul.ac.id/index.php/jsk/article/view/32

Sulistiyono, F. D., & Mahyuni, S. (2019). Isolasi Dan Identifikasi Jamur Endofit Pada Umbi Talas (Colocasia esculenta (L.) Schoot). Jurnal Sains Natural, 9(2), 66-70. https://repository.unpak.ac.id/tukangna/repo/file/files-20201208112200.pdf

Septiana, E. (2017). Bakteri Endohifa: Si Kecil di Balik Besarnya Manfaat Kapang Endofit. Biotrends, 8(1), 10-16. file:///C:/Users/ASUS/Downloads/198-363-1-SM.pdf

Sudantha, I. M., & Abadi, A. L. (2011). Uji Efektivitas Beberapa Jenis Jamur Endofit Trichoderma spp. Isolat Lokal NTB Jamur Fusarium oxysporum f.sp. vanillae Penyebab Penyakit Busuk pada Bibit vanili. Agroteksos,4(2): 64-73. DOI :https://scholar.google.co.id/citations?user=dhVzR6gAAAAJ&hl=id

Strobel, G., Daisy, B., Castillo, U., & Harper, J. (2004). Natural products from endophytic microorganisms. Journal of Natural products, 67(2), 257-268. https://pubs.acs.org/doi/abs/10.1021/np030397v

Aditya, K., & Kumar, A. R. (2014). Phytochemical evaluation of Vitex leuocoxylon, vitex negundo and vitex trifolia. Indian Journal of Research in Pharmacy and Biotechnology, 2(2), 1106. https://www.ijrpb.com/issues/Volume%202_Issue%202/ijrpb%202(2)%206%20K%20Aditya1%201106-1108.pdf

Mary, R. N. I., Meenashree, B., & Vasanthi, V. J. (2014). Screening of antibacterial activity and qualitative and quantitative analysis of phytochemicals in Vitex trifolia. Int. J. Curr. Microbiol. Appl. Sci, 3, 425-431. https://www.researchgate.net/profile/Meenashree_Balakrishnan/publication/325464368

Maia, M.F. & Moore, S.J. (2011). Plant-Based Insect Repellents: A Review of Their Efficacy, Development and Testing. Malaria Journal. 10(Suppl 1):S11. DOI: https://www.researchgate.net/publication/50408598

Berdy, J. (2005). Review Article: Bioactive Microbial Metabolites. The Journal of Antibiotics, 58(1): 1-26. DOI :https://www.nature.com/articles/ja20051

Azevedo, J.L. Maccheroni, W. Pereira, J.O. & Araujo, W.L.D. (2000). Endophytic Microorganism: A Review in Insect Control and Recent Advances on Tropical Plants. Electron Journal of Biotechnology, 3(1): 40-65. ISSN: 0717- 3458. DOI: http://www.bioline.org.br/pdf?ej00005

Yaqub, F. and Shahzad, S. (2005). Pathogenicity of Sclerotium rolfsii in different crops and effect of inoculums density on colonization of mungbean and sunflower roots. Pak J. Bot. 37(1): 175-180. DOI: https://www.researchgate.net/publication/266468138

Prasati, O.H., Kristanti, I., & Nurhatika,S. (2013) Pengaruh Mikoriza Glomusfasciculatum Terhadap Pertumbuhan Vegetatif Tanaman Kacang Tanah yang Terinfeksi Patogen Sclerotiumrolfsii. Jurnal sains dan seni pomits. 2(2):74 Ã¢â¬â 78. DOI: http://ejurnal.its.ac.id/index.php/sains_seni/article/view/3624

Sofia, D. (2001). Pengaruh Pestisida Dalam Lingkungan Pertanian. Sumatera Utara: USU. DOI: http://repository.usu.ac.id/handle/123456789/1106

Tenrirawe, A dan A.H. Talanca. 2008. Bioekologi dan Pengendalian Hama dan Penyakit Utama Kacang Tanah. Prosiding: 464 Ã¢â¬â 471. DOI: https://id.scribd.com/doc/75956645/54-Tenrirawe-Pengendalian-Penyakit-K-tanah-464-471-2

Sudantha, I.M & A.L Abadi (2007). Identifikasi Jamur Endofit dan Mekanisme Antagonismenya terhadap Jamur Fusarium oxysporum f. sp vanillae Pada Tanaman Vanili. Agroteksos. 17 (1). DOI: https://www.semanticscholar.org/paper/identifikasi-jamur-endofit-dan-mekanisme-terhadap-Sudantha-Abadi/7228d53b821f48c5051f9dd950c9f8210d8332e4

Gandjar, I. Samson, R. A., Van Den Tweel-Vermeulen, K. Oetari, A. & Santoso, I. (1999). Pengendalian Kapang Tropik Umum. Yayasan Obor Indonesia. Jakarta. ISBN 979-461-289-8

Suryaningsih, E & W. Hadisoeganda (2012). Pestisida Botani untuk Mengendalikan Hama dan Penyakit pada Tanaman Sayuran. Bandung. Mitra Buana Pasundan

Rahman, M. A., Begum, M. F., & Alam, M. F. (2009). Screening of Trichoderma isolates as a biological control agent against Ceratocystis paradoxa causing pineapple disease of sugarcane. Mycobiology, 37(4), 277-285. DOI: https://doi.org/10.4489/MYCO.2009.37.4.277

Iien, H., Zulkifli, L., & Prapti, S. (2020). Aktivitas antibakteri ekstrak metanol daun turi (Sesbania grandifora L) terhadap pertumbuhan Klebsilla pneumonia. Jurnal Biologi Tropis, 20 (1): 219-225. DOI: http://dx.doi.org/10.29303/jbt.v20i2.1790

Barnett, H.L., and Hunter, B.B. (1978). Ilustrated Genera of Imperfect Fungi Fourth Edition. APS Press. Minnessota

Watanabe, S. (2002). Pictorial Atlas of Soil and Seed Fungi Morphologies of Cultured Fungi and Key to Species Second Edition. CRC Press. Washington, D.C. ISBN 0-8493-1118-7.

Mulyani, R. B., Usup, A., Supriati, L, & Ramlan. (2018). Peran Agen Hayati Asal Rizosfer Dan Endofit Menekan PenyakitBusuk Sklerotium rolfsii Bawang Daun Di Media Gambut. Jurnal AGRI PEAT, 19 (2). ISSN: 1411 Ã¢â¬â 6782 (Cetak) 2620-6935 (Elektronik. DOI: https://e-journal.upr.ac.id/index.php/Agp/article/view/159

Arios, L. N., Kiki, K., Erman, M., & Dwi, S. (2014). Asai kemampuan bakteri endofit dari kacang tanah dalam menghambat pertumbuhan sclerotium sp. Pada kecambah kacang tanah. Jurnal Hama dan Penyakit Tumbuhan Tropika, 14(2). DOI: https://doi.org/10.23960/j.hptt.214178-186

Arnold, A.E, Mejia, L.C., Kyllo, D., Rojas, E.L, Maynard, Z., Robbins, N., & Herre, E.A. (2003). Fungal endophytes limit pathogen damage in a tropical tree. PNAS, 100, 15649-15654. DOI: https://doi.org/10.1073/pnas.2533483100

Kalay, A.M., Abraham, T., & Wilhemina, R. (2018). Uji Antagonisme Trichodermaharzianum dan Azotobacterchroococcum terhadap Rhyzoctoniasolani, Sclerotiumrolfsii dan FusariumOxysporum Secara in-Vitro. Agrogolia, 7(2). DOI: http://dx.doi.org/10.30598/a.v7i2.764

Liu, C.H., Zou, W.X., Lu, H., & Tan, R.X. (2001). Antifungal Activity of Artemisia annua endophyte cultures against phytopathogenic fungi. J Biotechnol, 88, 277-282. DOI: https://doi.org/10.1016/S0168-1656(01)00285-1

Maulani, B. I. G., Rasmi, D. A. C., & Zulkifli, L. (2019). Isolation and characterization of endophytic bacteria from mangrove Rhizophora mucronata Lam. and antibacterial activity test against some pathogenic bacteria. In Journal of Physics: Conference Series, 1402 (3), p. 033038. IOP Publishing. https://iopscience.iop.org/article/10.1088/1742-6596/1402/3/033038

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Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria

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