The Effectiveness Probiotic Drink of Salak Bali (Salacca zalacca) in Inhibiting Growth of Escherichia coli
Authors
Nadya Treesna Wulansari , A.A.Istri Mas Padmiswari , Ida Ayu Manik DamayantiDOI:
10.29303/jbt.v22i3.3515Published:
2022-09-12Issue:
Vol. 22 No. 3 (2022): July - SeptemberKeywords:
Escherichia coli, inhibition, probiotics, salak baliArticles
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Abstract
Salak bali (Salacca zalacca) is one of the local fruit varieties cultivated in Bali. This fruit contains antioxidants, so that it is potential to be used as a probiotic drink. Probiotic drinks containing live microbes can inhibit the growth of pathogenic microbes and balance the microbes in the intestines so that they can affect the health of those who consume them. This study aimed to determine the effectiveness of the probiotic drink of salak bali fruit juice (Salacca zalacca) in inhibiting the growth of Escherichia coli. The study employed experimental with a completely randomized design. Salak juice was fermented for 18 days and harvested every 3 days, then an antibacterial test was carried out. The data were analyzed by using analysis of variance (ANOVA) followed by Duncan's Multiple Range Test (DMRT) at a 5% significance level. The finding showed that probiotic drink of salak bali fruit juice effective to inhibiting the growth of Escherichia coli. Optimal antibacterial growth was seen on the 15th day of fermentation with an inhibition zone of 8.13 ± 0.40 mm. The presence of inhibition caused by the phytochemical content found in salak fruit, SCOBY contained in drinks, and the length of the fermentation process.
References
Arivianti, S., & Parnanto, N. H. R. (2013). Kapasitas antioksidan buah salak (Salacca edulis REINW) kultivar pondoh, nglumut dan Bali serta korelasinya dengan kadar fenolik total dan vitamin C. Agritech, 33(3), 324-333. DOI: https://doi.org/10.22146/agritech.9555.
Coton, M., Pawtowski, A., Taminiau, B., Burgaud, G., Deniel, F., Coulloumme-Labarthe, L., & Coton, E. (2017). Unraveling microbial ecology of industrial-scale Kombucha fermentations by metabarcoding and culture-based methods. Fems Microbiology Ecology, 93(5), 1–16. https://doi.org/10.1093/femsec/fix048.
Faizin, M & Fidyasari, A. (2019). Uji Aktivitas Antibakteri Dari Minuman Probiotik Sirsak Gunung (Annona montana Macf.) Dalam Menghambat Pertumbuhan Bakteri Salmonella sp. Diploma thesis, Akademi Farmasi Putera Indonesia Malang.
Fransisca, D., Kahanjak, D. N., & Frethernety, A. (2020). Uji aktivitas antibakteri ekstrak etanol daun sungkai (Peronema canescens Jack) terhadap pertumbuhan Escherichia coli dengan metode difusi cakram Kirby-Bauer. Jurnal Pengelolaan Lingkungan Berkelanjutan (Journal of Environmental Sustainability Management), 4 (1): 460-470. DOI: https://doi.org/10.36813/jplb.4.1.460-470.
Faizain, M. & Fidyasari, A. (2019). Aktivitas Antibakteri dari Minuman Probiotik Sirsak Gunung (Annona montana Macf.) Dalam Menghambat Pertumbuhan Bakteri Salmonella sp. Karya Tulis Ilmiah. Akademi Farmasi Putra Indonesia Malang. Retrieved from http://repository.poltekkespim.ac.id/id/eprint/441/1/ARTIKEL%20KTI.pdf
Fuller, R. (1999). Probiotics for Farm Animals. Tannock, G.W., editor. New York: Horizon Scientific Press.
Hartmann, M., Berditsch, M., Hawecker, J., Ardakani, M. F., Gerthsen, D., & Ulrich, A. S. (2010). Damage of the bacterial cell envelope by antimicrobial peptides gramicidin S and PGLa as revealed by transmission and scanning electron microscopy. Antimicrobial agents and chemotherapy, 54(8), 3132-3142.
Harmita & M. Radji. (2008). Kepekaan Antibiotik dalam Buku Ajar Analisis Hayati Ed. 3, EGC, Jakarta, pp. 1- 5.
Hull Vance, S., Tucci, M., & Benghuzzi, H. (2011). Evaluation of the antimicrobial efficacy of green tea extract (egcg) against Streptococcus pyogenes in vitro - biomed 2011. Biomedical sciences instrumentation, 47, 177–182.
Ismadi, I. T., Pestariati, P., & Astuti, S. S. E. (2019). Uji Antibakteri Ekstrak Buah Salak (Salacca Edulis) Pada Pertumbuhan Bakteri Salmonella Typhi Secara In Vitro. Analis Kesehatan Sains, 8(1).
Jang, J., Hur, H. G., Sadowsky, M. J., Byappanahalli, M. N., Yan, T., & Ishii, S. (2017). Environmental Escherichia coli: ecology and public health implications-a review. Journal of applied microbiology, 123(3), 570–581. DOI: https://doi.org/10.1111/jam.13468.
Kaczmarek, A. (2020). Tannic Acid with Antiviral and Antibacterial Activity as A Promising Component of Biomaterials—A Minireview. Materials (13) DOI: https://doi.org/10.3390/ma13143224.
Koesoemawardani D., S. Rizal & M. Tauhid. (2013). Perubahan sifat mikrobiologi dan kimiawi rusip selama fermentasi. Agritech 33(3): 265-272. Jurusan Teknologi Hasil Pertanian Fakultas Pertanian, Universitas Lampung.
Kusuma, S.A.F. (2010). Escherichia coli. Makalah Fakultas Farmasi Universitas Padjajaran, Bandung.
Marsh, A. J., O’Sullivan, O., Hill, C., Ross, R. P., & Cotter, P. D. (2014). Sequence-based analysis of the bacterial and fungal compositions of multiple Kombucha (tea fungus) samples. Food Microbiology, 38, 171–178. https://doi.org/10.1016/j.fm.2013.09.003.
Nurina, C., Samingan, S., & Iswadi, I. (2015). Uji Antimikroba Ekstrak Buah Salak (Salacca edulis) Terhadap Bakteri Escherichia Coli. Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi, 6(1), 19-23. Retrieved from http://jurnal.unsyiah.ac.id/JBE/article/view/2271.
Othman L, Sleiman A & Abdel-Massih RM (2019) Antimicrobial Activity of Polyphenols and Alkaloids in Middle Eastern Plants. Front. Microbiol. 10 (911): 1-28 DOI: 10.3389/fmicb.2019.00911.
Pallant, Jullie. 2010. SPSS Survival Manual 4 th Edition. New York: Mc Graw Hill.
Radji, M. (2011). Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi dan Kedokteran. Jakarta: Penerbit Buku Kedokteran EGC.
Rante, B. K., Assa, Y. A., & Gunawan, P. N. (2017). Uji daya hambat getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan Staphylococcus aureus. e-GiGi, 5(2).
Tannock, G.W. (2005). Probiotics and Prebiotics: Scientific Aspects. New York: Horizon Scientific Press. pp : 43.
Rahmiati & Simanjuntak, H.A (2019). Kemampuan Bakteri Asam Laktat Dalam Menghambat Salmonella thypii. Jurnal Jeumpa, 6 (2): 257-264. DOI: https://doi.org/10.33059/jj.v6i2.1898.
Rojas-Lopez M, Monterio R, Pizza M, Desvaux M and Rosini, R. (2018). Intestinal Pathogenic Escherichia coli: Insights for Vaccine Development. Front. Microbiol. 9:440. DOI: https://doi.org/10.3389/fmicb.2018.00440.
Roos, J. D., & Vuyst, L. D. (2018). Acetic acid bacteria in fermented foods and beverages. Current Opinion in Biotechnology. 49, 115–119. DOI: https://doi.org/10.1016/j.copbio.2017.08.007.
Suprihatin. (2010). Teknologi Fermentasi. Surabaya: UNESA University Press.
Yunus, Y., & Zubaidah, E. (2014). Pengaruh Konsentrasi Sukrosa Dan Lama Fermentasi Terhadap Viabilitas L. Casei Selama Penyimpanan Beku Velva Pisang Ambon [In Press April 2015]. Jurnal Pangan dan Agroindustri, 3(2), 303-312.
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