Antibacterial and Cytotoxic Activity from Ethyl Acetate Extract of Sargassum from Kabung Island West Kalimantan
DOI:
10.29303/jbt.v23i1.4560Published:
2023-01-10Downloads
Abstract
Sargassum belongs to Phaeophyceae is widely distributed in Indonesian waters, including Kabung Island. Seaweed has bioactive compounds that potential to be developed. In the health sector, one of the global problems that still increasing is the bacterial infection. This condition leads us to explore antibiotic from natural resources to overcome this problem. Sargassum grow abundantly in the waters of Kabung Island and this genus has not been utilized by local community. For this reason, the aim of this study is to evaluate the antibacterial and cytotoxic activity from ethyl acetate extract of Sargassum from Kabung Island, West Kalimantan. Antibacterial activity test of the ethyl acetate extract against E. coli and S. aureus was conducted using the diffusion method with concentration series of 100; 50; 25; 5; 1; and 0.1 g/mL. While, the assessment of cytotoxic activity was carried out with concentration of 10; 100 and 1000 g/mL. The result showed that ethyl acetate extract of Sargassum from Kabung Island only showed antibacterial activity against E. coli bacteria. The greatest inhibition zone was 11.59 mm at a concentration of 100 g/mL, while in the cytotoxic test exhibited that the LC50 was more than 1000 ppm, so it was considered into non-toxic category.
Keywords:
antibacterial; cytotoxic; ethyl acetate; sargassum; Kabung Island, West KalimantanReferences
Adam, N., Lolo, W.A., Sudewi, S. (2019). Aktivitas Antibakteri Fraksi Alga Turbinaria ornata (Turner) J. Agardh yang Diperoleh dari Perairan Teluk Manado. J. Pharmacon, 8(2): 325-334. DOI: https://doi.org/10.35799/pha.8.2019.29298
Alamsjah, M.A., Nurhayati, D., Tjahjaningsih. (2011). Pengaruh Ekstrak Alga Cokelat (Sargassum sp.) terhadap Pertumbuhan Bakteri Escherichia coli Secara in Vitro. J. Ilmiah Perikanan dan Kelautan, 3(2): 69-78. DOI: 10.20473/jipk.v3i1.11627
Anggadiredja, J.T.; Zatnika, A.; Purwoto, H.; dan Istini, S. (2008). Rumput Laut, Pembudidayaan, Pengolahan, dan Pemasaran Komoditas Perikanan Potensial. Jakarta: Penebar Swadaya. 147 hlm.
Baud, Grace S., Meiske, S., Sangi, Harry, S.J., Koleangan. (2014). Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Etanol Batang Tanaman Patah Tulang (Euphorbia tirucalli L.) dengan Metode Brine Shrimp Lethality Test (BSLT). J. Ilmiah Sains., 14(2): 106-112. DOI: https://doi.org/10.35799/jis.14.2.2014.6065
Handayani, P.A. dan Nurcahyanti, H. (2015). Ekstraksi Minyak Atsiri Daun Zodia (Evodia suaveolens) dengan Metode Maserasi dan Distilasi Air. J. Bahan Alam Terbarukan. 4(1): 1-7. DOI: https://doi.org/10.15294/jbat.v3i1.3095
Herawati, D. and Pudjiastuti, P. (2021). Effect of Different Solvents on The Phytochemical Compounds of Sargassum sp. from Yogyakarta and East Nusa Tenggara. Journal of Physics: Conference Series, 1783: 012001. DOI 10.1088/1742-6596/1783/1/012001
John, D.M. and Al-Thani, R.F. (2014). Benthic marine algae of the Arabian Gulf: a critical review and analysis of distribution and diversity patterns. Nova Hedwigia, 98(3-4): 341-392. DOI:10.1127/0029-5035/2014/0156
Khanavi, M., Nabavi, M., Sadati, N., Ardekani, M.S., Sohrabipour, J., Nabavi, S.M.B., Ghaeli, P., Ostad, S.N. (2010). Cytotoxic activity of some marine brown algae against cancer cell lines. Biol. Res., 43: 31-37. DOI: doi.org/10.4067/S0716-97602010000 100005
Lekameera, Vijayabaskar, R.P., Somasundaram, S.T. (2008). Evaluating antioxidant property of brown ALGA Colpomenia sinuosa (DERB. ET SOL). African Journal of Food Science, 2: 126-130. https://www.researchgate.net/publication/237740016
Lidiawati, T.E., Saleh, C., Alimuddin. (2018). Sintesis Etil Asetat dari Hasil Fermentasi Kulit Singkong (Manihot esculenta L.) dengan Asam Asetat Menggunakan Katalis Asam. Prosoding Seminar Nasional Kimia, 82-86. URL: http://jurnal.kimia.fmipa.unmul.ac.id/index.php/prosiding/article/view/764>.
Lomartire, S. and Gonçalves, A.M.M. (2022). Antiviral Activity and Mechanisms of Seaweeds Bioactive Compounds on Enveloped Viruses - A Review. Mar. Drugs., 20: 385. DOI: 10.3390/ md20060385.
Monla, R.A., Dassouki, Z., Kouzayha, A., Salma, Y., Gali-Muhtasib, H., Mawlawi, H. (2020). The Cytotoxic and Apoptotic Effects of the Brown Algae Colpomenia sinuosa are Mediated by the Generation of Reactive Oxygen Species. Molecule, 25: 1993. DOI: 10.3390/molecules25081993
Muaja, A.D., Koleangan, H.S.J., Runtuwene, M.R.J. (2013). Uji Toksisitas dengan Metode BSLT dan Analisi Kandungan Fitokimia Ekstrak Daun Soyogik (Saurauia bracteosa DC) dengan Metode Soxhletasi. J. MIPA Unsarat, 2(2): 115-118. DOI: doi.org/10.35799/jm.2.2.2013.3000
Muharani, Fitrya, Farida, S. (2017). Uji Aktivias Antibakteri Ekstrak Etanol Tanaman Obat Suku Musi di Kabupaten Musi Bayuasin Sumatera Selatan. J. Kefarmasian Indonesia, 7(2): 127-135. DOI: https://doi.org/10.22435/jki.v7i2.3493
Naina, Y., Wulandari, R., Raza’i, T.S. (2019). Skrining Komponen Bioaktif Ethanol 96% Sargassum sp. sebagai Antibakteri terhadap Vibrio harveyi. J. Intek Akuakultur, 3(2): 22-33. DOI:10.31629/ INTEK.V3I2.1360
Rastina, Sudarwanto, M., Wientarsih, I. (2015). Aktivitas Antibakteri Ekstrak Etanol Daun Kari (Murraya koenigii) terhadap Staphylococcus aureus, Escherichia coli, dan Pseudomonas sp. J. Kedokteran Hewan, 9(2): 185-188. DOI: doi.org/10.21157/ j.ked.hewan.v9i2.2842
Safitri, I., Warsidah, Sofiana, M.S.J., Kushadiwijayanto, A.A., Sumarni, T.N. (2021). Total Phenolic Content, Antioxidant and Antibacterial Activities of Sargassum polycystum of Ethanol Extract from Waters of Kabung Island. BERKALA SAINSTEK, 9(3): 139-145. DOI: https://doi.org/10.19184/bst.v9i3.27199
Sanger, G., Rarung, L.K., Wonggo, D., Dotulong, V., Damongilala, L.J., Tallei, T.E. (2021). Cytotoxic activity of seaweeds from North Sulawesi marine waters against cervical cancer. Journal of Applied Pharmaceutical Science, 11(09): 066-073. DOI: 10.7324/JAPS.2021.1109 08
Sathya, R., Kanaga, N., Sankar, P., Jeeva, S. (2017). Antioxidant Properties of Phlorotanninsfrom Brown Seaweed Cystoseira trinodis (Forsskal) C. Agardh. Arabian J. Chem., 10: 2608-2614. DOI:10.1016/j.arabjc.2013.09.039
Savitri, I., Suhendra, L., Wartini, N.D. (2017). Pengaruh Jenis Pelarut pada Metode Maserasi terhadap Karakteristik Ekstrak Sargassum polycatum. J. Rekayasa dan Manajemen Agroindustri. 5(3): 93-101. https://ojs.unud.ac.id/index.php/jtip/article/view/35504
Sidauruk, S.W., Sari, N.I., Diharmi, A., Arif, I. (2021). Aktivitas Antibakteri Ekstrak Sargassum plagyophyllum terhadap Bakteri Listeria monocytogenes dan Pseudomonas aeruginosa. J. PHPI., 24(1): 27-37. DOI: https://doi.org/10.17844/ jphpi.v24i1.33417
Sinurat, A.A.P., Renta, P.P., Herliany, N.E., Negara, B.F., Purnama, D. (2019). Uji Aktivitas Aantibakteri Ekstrak Metanol Rumbut Laut Gracilaria edulis terhadap Bakteri Aeromonas hydrophil. J.Enggano, 4(1): 105-114. DOI: https://doi.org/10.31186/ jenggano.4.1.105-114
Siregar, A.F., Sabdono, A., Pringgenies, D. (2012). Potensi Antibakteri Ekstrak Rumput Laut terhadap Bakteri Penyakit Kulit Pseudomonas aeruginosa, Staphylococcus epidermidis dan Micrococcus inteus. Journal of Marine Research, 1(2): 152-160. DOI: https://doi.org/10.14710/jmr.v1i2.2032
Sofiana, M.S.J., Nurrahman, Y.A., Warsidah, Minsas, S., Yuliono, A., Safitri, I., Helena, S., Risko. (2022). Community Structure of Macroalgae in Lemukutan Island Waters, West Kalimantan. Jurnal Ilmu Kelautan SPERMONDE, 8(1): 1-8. DOI: https://doi.org/10.20956/jiks.v8i1.17914
Sudhakar, K., Mamat, R., Samykano, M., Azmi, W.H., Ishak, W.F.W., Yusaf, T. (2018). An overview of marine macroalgae as bioresource. Renewable and Sustainable Energy Reviews, 91: 165-179. DOI: https://doi.org/10.1016/j.rser.2018.03.100
Sumarni, T.N., Warsidah, Safitri, I., Kushadiwijayanto, A.A., Sofiana, M.S.J. (2022). Analisis Kandungan Proksimat dan Mineral Zink dari Sargassum sp. asal Perairan Pulau Kabung. Oseanologia, 1(1): 24-27. DOI: doi.org/10.26418/jose.v1i1. 53467
Szmolka, A. and Nagy, B. (2013). Multidrug resistant commensal Escherichia coli in animals and its impact for public health. Frontier in Microbiology, 4(258): 1-13. DOI: doi: 10.3389/fmicb.2013.00258
Vidotti, E.C., Rollemberg, M., Do, C.E. (2014). Algas: Da economia nos ambientes aquáticos à biorremediação e àquímica analítica. Quim. Nova, 27: 139-145. DOI: https://doi.org/10.1590/S0100-40422004 000100024
Warsidah, Safitri, I., Sofiana, M.S.J., Helena, S. (2022). Antibacterial Activity from Ethanol and Ethyl Acetate Extracts of Padina pavonica Hauck from Kabung Island against Escherichia coli. Saintek Perikanan: Indonesian Journal of Fisheries Science and Technology, 18(1): 1-6. DOI: https://doi.org/10.14710/ijfst.18.1.1-6
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