Biological Agent Trichoderma asperellum and Its in Vitro Inhibitory Activity Against Mango Fruit Rot Pathogens

Gilang Vaza Benatar; Yeyet Nurhayati; Umi Kulsum

doi:10.29303/jbt.v23i3.4982

Biological Agent Trichoderma asperellum and Its in Vitro Inhibitory Activity Against Mango Fruit Rot Pathogens

Authors

Gilang Vaza Benatar , Yeyet Nurhayati , Umi Kulsum

DOI:

10.29303/jbt.v23i3.4982

Published:

2023-05-22

Issue:

Vol. 23 No. 3 (2023): July - September

Keywords:

dual culture, morphology, molecular technique, plant disease.

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Benatar, G. V., Nurhayati, Y., & Kulsum, U. (2023). Biological Agent Trichoderma asperellum and Its in Vitro Inhibitory Activity Against Mango Fruit Rot Pathogens. Jurnal Biologi Tropis, 23(3), 70–75. https://doi.org/10.29303/jbt.v23i3.4982

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Abstract

Diseases are one of the major challenges in crop production which can lead to significant economic losses. Ironically, while having multiple negative impacts, synthetic chemical pesticides are considered the main strategy in tackling plant diseases. Trichoderma asperellum is one of the potential fungal species that can inhibit numerous plant diseases. This study reconfirmed the morphological characteristics of Trichoderma isolates obtained and identified it by molecular techniques. In addition, the inhibitory activity of the isolates was tested in vitro against the mango pathogens Colletotrichum asianum and Diaporthe pseudophoenicicola. The results showed that isolate T4 was identified as Trichoderma asperellum. Isolate T4 was able to inhibit C. asianum and D. pseudophoenicicola by 28.19% and 15.64%, respectively. Therefore, this isolate can be used as a biological agent to control plant pathogens in an environmentally friendly, sustainable manner, and as an alternative to synthetic chemical pesticides.

References

Anees, M., Azim, R., Ur Rehman, S., Jamil, M., El Hendawy, S. E., & Al-Suhaiban, N. A. (2018). Antifungal potential of Trichoderma strains originated from north western regions of Pakistan against the plant pathogens. Pakistan Journal of Botany, 50(5), 2031–2040.

Bruns, T. D., Lee, S. B., & Taylor, J. W. (1990). White , T . J ., T . D . Bruns , S . B . Lee , and J . W . Taylor . Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics. May 2014.

Ferreira, F. V., & Musumeci, M. A. (2021). Trichoderma as biological control agent: scope and prospects to improve efficacy. World Journal of Microbiology and Biotechnology, 37(5), 1–17. https://doi.org/10.1007/s11274-021-03058-7

He, D. C., He, M. H., Amalin, D. M., Liu, W., Alvindia, D. G., & Zhan, J. (2021). Biological control of plant diseases: An evolutionary and eco-economic consideration. Pathogens, 10(10). https://doi.org/10.3390/pathogens10101311

Islam, S., Siddique, S., Chowdhury, M., & Mishu, N. (2021). Inhibitory Effect of Trichoderma asperellum Isolate against Ralstonia solanacearum Causing Brinjal Wilt. Annals of Bangladesh Agriculture, 24(2), 107–120. https://doi.org/10.3329/aba.v24i2.55788

Ismaiel, M. H., El Zanaty, A. M., & Abdel-Lateif, K. S. (2022). Molecular and Morphological Identification of Trichoderma Isolates From Egyptian Agriculture Wastes-Rich Soil. Sabrao Journal of Breeding and Genetics, 54(3), 598–607. https://doi.org/10.54910/sabrao2022.54.3.12

Katoch, A., & Kapoor, P. (2014). Recent Concepts in Fungal Taxonomy: A Review. Research & Reviews: Journal of Agriculture and Allied Sciences, 3(2), 23–35. http://www.rroij.com/open-access/recent-concepts-in-fungal-taxonomy-a-review.php?aid=33856

Khalid, S. A. (2017). Trichoderma as biological control weapon against soil borne plant pathogens. African Journal of Biotechnology, 16(50), 2299–2306. https://doi.org/10.5897/ajb2017.16270

Li, N., Alfiky, A., Wang, W., & Nourollahi, K. (2018). Volatile Compound-Mediated Recognition and Inhibition Between Trichoderma Biocontrol Agents and Fusarium oxysporum. 9(October), 1–16. https://doi.org/10.3389/fmicb.2018.02614

Liu, X., Min, W., Mei, S., Wang, L., & Jiang, S. (2021). Plant Disease Recognition: A Large-Scale Benchmark Dataset and a Visual Region and Loss Reweighting Approach. IEEE Transactions on Image Processing, 30, 2003–2015. https://doi.org/10.1109/TIP.2021.3049334

Mukhopadhyay, R., & Kumar, D. (2020). Trichoderma: a beneficial antifungal agent and insights into its mechanism of biocontrol potential. Egyptian Journal of Biological Pest Control, 30(1). https://doi.org/10.1186/s41938-020-00333-x

Nurhayati, Y., Suryanti, S., & Wibowo, A. (2021). In Vitro Evaluation of Trichoderma asperellum Isolate UGM-LHAF against Rhizoctonia solani Causing Sheath Blight Disease of Rice. Jurnal Perlindungan Tanaman Indonesia, 25(1), 64. https://doi.org/10.22146/jpti.65290

Pandit, M. A., Kumar, J., Gulati, S., Bhandari, N., Mehta, P., Katyal, R., Rawat, C. D., Mishra, V., & Kaur, J. (2022). Major Biological Control Strategies for Plant Pathogens. Pathogens, 11(2), 1–21. https://doi.org/10.3390/pathogens11020273

Salem-Bango, Z., Price, T. K., Chan, J. L., Chandrasekaran, S., Garner, O. B., & Yang, S. (2023). Fungal Whole-Genome Sequencing for Species Identification: From Test Development to Clinical Utilization. Journal of Fungi, 9(2). https://doi.org/10.3390/jof9020183

Siddiquee, S. (2017). Practical Handbook of the Biology and Molecular Diversityof Trichoderma Species from Tropical Regions, Fungal Biology.

Thavaprakasapandian, R., Raja, M., Kumar, A., & Sharma, P. (2016). Morphological and molecular characterization of Trichoderma asperellum strain Ta13. Indian Phytopathology, 69(3), 298–303.

Tilocca, B., Cao, A., & Migheli, Q. (2020). Scent of a Killer: Microbial Volatilome and Its Role in the Biological Control of Plant Pathogens. Frontiers in Microbiology, 11(February). https://doi.org/10.3389/fmicb.2020.00041

Wang, H., Zhang, R., Duan, Y., Jiang, W., Chen, X., Shen, X., Yin, C., & Mao, Z. (2021). The endophytic strain trichoderma asperellum 6s-2: An efficient biocontrol agent against apple replant disease in china and a potential plant-growth-promoting fungus. Journal of Fungi, 7(12). https://doi.org/10.3390/jof7121050

Zhang, C., Wang, W., Xue, M., Liu, Z., Zhang, Q., Hou, J., Xing, M., Wang, R., & Liu, T. (2021). The combination of a biocontrol agent trichoderma asperellum sc012 and hymexazol reduces the effective fungicide dose to control fusarium wilt in cowpea. Journal of Fungi, 7(9). https://doi.org/10.3390/jof7090685

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Biological Agent Trichoderma asperellum and Its in Vitro Inhibitory Activity Against Mango Fruit Rot Pathogens

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