Ability of Melon (Cucumis melo. L) Fruit Juices Based Tris Diluent on The Quality of Frozen Spermatozoa of Bali Cattle After Thawing
Authors
Mujahidurrohman Mujahidurrohman , Enny Yuliani , Lukman HYDOI:
10.29303/jbt.v23i3.5380Published:
2023-07-16Issue:
Vol. 23 No. 3 (2023): July - SeptemberKeywords:
Bali cattle, melon juice, semen quality.Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
The high potassium content in melon plays a role in maintaining the normal function of the muscles, heart, nervous system, and potassium is also the main regulator of blood pressure. The aim of this study was to evaluate the effect of using melon juice (Cucumis melo L.) as a diluent on the quality of spermatozoa and semen of Bali cattle both before and after freezing. The Banyumulek Artificial Insemination Center produces sperm used in Bali cattle breeding. The technique used was a completely randomized design (CRD) with 4 treatments and 15 replications. The first treatment was 100% egg yolk tris buffer without the addition of melon juice (P0), the second treatment was 80% buffer without the addition of 20% melon juice (P1), the third treatment was 60% buffer with the addition of 40% melon juice (P2). The fourth treatment was 40% buffer with the addition of 60% melon juice (P3). The outcomes showed that the worth of flawless plasma film and mortality upsides of ox-like semen after weakening and subsequent to defrosting were not essentially unique. Be that as it may, not altogether disparate in that frame of mind of motility, level of reasonability, level of ordinariness and post-defrosting anomalies. According to the findings of this study, melon juice can be used as an alternative extender for fresh Bali cattle sperm and preserve the quality of spermatozoa.
References
Afiati, F., & Said, I. S. (2013). Pembibitan Ternak dengan Inseminasi Buatan. Penebar Swadaya Grup.
Aliyah, S. N., Santoso, H., & Zayadi, H. (2022). Analisis Normalitas dan Abnormalitas Spermatozoa Segar Sapi Limousin (Bos taurus) dan Sapi Bali (Bos sondaicus) Sebelum Proses Pembekuan Di Balai Besar Inseminasi Buatan Singosari Malang. Sciscitatio, 3(1), 38-46.
Allai, L., Druart, X., Contell, J., Louanjli, N., Moula, A. B., Badi, A., ... & El Amiri, B. (2015). Effect of argan oil on liquid storage of ram semen in Tris or skim milk based extenders. Animal reproduction science, 160, 57-67. DOI: https://doi.org/10.1016/j.anireprosci.2015.07.003
Anwar, A., Solihati, N., & Rasad, S. D. (2019). Pengaruh medium dan lama inkubasi dalam proses sexing sperma terhadap kualitas semen kambing Boer. Jurnal Ilmu Ternak Universitas Padjadjaran, 19(1), 53-61. DOI: https://doi.org/10.24198/jit.v19i1.23009
Anwar, P., Ondho, Y., & Samsudewa, D. (2014). Pengaruh pengencer ekstrak air tebu dengan penambahan kuning telur terhadap kualitas spermatozoa sapi Bali. Jurnal Peternakan, 11(2), 48-58. DOI: http://dx.doi.org/10.24014/jupet.v11i2.2719
Arifiantini, R. I., & Purwantara, B. (2010). Motility and viability of friesian holstein spermatozoa in three different extender stored at 5oC. Journal of the Indonesian Tropical Animal Agriculture, 35(4), 222-226. DOI: https://doi.org/10.14710/jitaa.35.4.222-226
Barek, M.E., Hine, T.M., Nalley, W.M. and Belli, H.L. (2020). Pengaruh Penambahan Sari Wortel Dalam Pengencer Sitrat Kuning Telur Terhadap Kualitas Spermatozoa Kambing Bligon (The effect of carrot juice supplementation in citrate-egg yolk extender on spermatozoa quality of bligon goat). Jurnal Nukleus Peternakan, 7(2), pp.109-117. DOI: https://doi.org/10.35508/nukleus.v7i2.3152
Barth, A. D., & Oko, R. J. (1989). Abnormal morphology of bovine spermatozoa. Iowa State University Press.
Bimanteri, L. and Widaryati, W. (2014). Pengaruh Pemberian Jus Melon Terhadap Tekanan Darah Penderita Hipertensi di Modinan Banyuraden Sleman Yogyakarta (Doctoral dissertation, Stikes'Aisyiyah Yogyakarta).
Blegur, J., Nalley, W.M. and Hine, T.M. (2020). Pengaruh Penambahan Virgin Coconut Oil Dalam Pengencer Tris Kuning Telur Terhadap Kualitas Spermatozoa Sapi Bali Selama Preservasi (Infiluence addition virgin coconut oil in tris egg yolk on the quality of Bali bull spermatozoa during preservation). Jurnal Nukleus Peternakan, 7(2), pp.130-138. DOI: https://doi.org/10.35508/nukleus.v7i2.2997
Bria, M.M., Nalley, W.M., Kihe, J.N. and Hine, T.M. (2022). Pengaruh Subtitusi Sari Buah Semangka (Citrullus Lanatus) Dalam Pengencer Sitrat-Kuning Telur Terhadap Kualitas Spermatozoa Sapi Bali (Effect of watermelon juice supplementation in citrate-egg yolk extender on spermatozoa quality of Bali bulls). Jurnal Nukleus Peternakan, 9(1), pp.23-32. DOI: https://doi.org/10.35508/nukleus.v9i1.4393
Dasrul, R. and Lubis, T.M., (2014). Pengaruh penambahan sari buah tomat dalam media pengencer terhadap motilitas dan viabilitas spermatozoa kambing Boer yang disimpan pada suhu 3–5 C. Jurnal Ilmiah Peternakan, p.97324.
Fadilah, Z.N., Isnaini, N. and Ihsan, M.N., (2016). Kualitas semen cair sapi Bali selama penyimpanan suhu ruang menggunakan pengencer skim milk dengan penambahan filtrat kecambah kacang hijau. Ternak Tropika Journal of Tropical Animal Production, 17(1), pp.22-30. DOI: https://doi.org/10.21776/ub.jtapro.2016.017.01.3
Fafo, M., Hine, T.M. and Nalley, W.M. (2016). Pengujian efektivitas ekstrak daun kelor dalam pengencer sitrat-kuning telur terhadap kualitas semen cair babi landrace. Jurnal Nukleus Peternakan, 3(2), pp.184-195. DOI: https://doi.org/10.35508/nukleus.v3i2.805
Feradis. (2010). Bioteknologi Reproduksi pada Ternak. Alfabeta, Bandung.
Garner, D. L & Hafez, E.S.E., (2000). Spermatozoa and Seminal Plasma. In Reproduction in Farm Animal Ed ke-7. In ESE Hafez (ed). Lea and Febiger Publishing, Philadelphia.
Garner, D. L. & Hafez, E.S.E., (2016). Spermatozoa and Seminal Plasma, in Reproduction in Farm Animals. Lippincott Williams & Wilkins. Baltimore, Maryland, USA. pp. 96-109.
Gordon, I. (2017). Reproductive Technologies in Farm Animals 2nd Edition. University College Dublin. Ireland.
Harahap, A. E., Handoko, J., Rodiallah, M., & Arman, C. (2020). Quality of Bali bull cryopreserved sperm using different extenders and equilibration times on pregnancy rate of Bali cows. Songklanakarin Journal of Science & Technology, 42(3).
Harahap, A.E., Handoko, J., Rodiallah, M. and Arman, C., 2020. Quality of Bali bull cryopreserved sperm using different extenders and equilibration times on pregnancy rate of Bali cows. Songklanakarin Journal of Science & Technology, 42(3).
Hardiyanti, H. and Kurniawan, M.E., (2020). Pengaruh Penambahan Sari Wortel (Daucus Carota, L) Pada Pengencer Terhadap Kualitas Spermatozoa Ayam Kampung Selama Penyimpanan. Musamus Journal of Livestock Science, 3(2), pp.12-20.
Hastuti, D.W.B. and Riviani, R., (2020). Efektifitas Penggunaan Jenis Ekstender dan Dosis Madu Berbeda Terhadap Motilitas dan Viabilitas Sperma Ikan Nilem (Osteochilus vittatus) Setelah Penyimpanan. Jurnal Airaha, 9(02), pp.122-129. DOI: https://doi.org/10.15578/ja.v9i02.183
Kune, P., Uly, K. and Nalley, W.M., (2021). Pengaruh Penambahan Filtrat Kelopak Bunga Rosella (Hibiscus sabdariffa Linn) dalam Pengencer Tris-Kuning Telur terhadap Kualitas Spermatozoa Sapi Bali: The Effect of Adding Rosella Flower Petal Filrate (Hibiscus Sabdariffa Linn) to Tris-Egg Yolk Diluent on The Quality of Bali Bull Spermatozoa. Jurnal Peternakan Lahan Kering, 3(1), pp.1309-1323.
Kusumawati, E.D., Betu, H., Krisnaningsih, A.T.N. and Rahadi, S., (2018). Kualitas semen segar sapi Limousin pada lama simpan yang berbeda. Jurnal Ilmiah Fillia Cendekia, 3(1), pp.1-9. DOI: https://doi.org/10.32503/fillia.v3i1.162
Labetubun, J. and Siwa, I.P., (2011). Kualitas spermatozoa kauda epididimis sapi Bali dengan penambahan laktosa atau maltosa yang dipreservasi pada suhu 3-5ºC. Jurnal Veteriner, 12(3), pp.200-207.
Lestari, S.D., Tagama, T.R. and Saleh, D.M., (2013). Profil produksi semen segar sapi simmental pada tingkat umur yang berbeda di balai inseminasi buatan lembang jawa barat. Jurnal Ilmiah Peternakan, 1(3), pp.897-906.
MataHine, T. and Burhanuddin, M.A., (2014). Efektivitas air buah lontar dalam mempertahankan motilitas, viabilitas dan daya tahan hidup spermatozoa sapi Bali. Jurnal veteriner, 15(2), pp.263-273. DOI: http://dx.doi.org/10.33772/jitro.v6i2.5936
Mokoagow, F., Pudjihastuti, E., Hendrik, M.J. and Paputungan, U., 2021. Makroskopik semen segar kambing bangsa Peranakan Etawa (PE), Boer dan Saanen di Balai Inseminasi Buatan Lembang. Zootec, 41(1), pp.150-157. DOI: https://doi.org/10.35792/zot.41.1.2021.32462
Muhammad, R., Abdillah, H., Ika, S. and Muhammad, R., (2017). Kemampuan Sari Melon Dalam Mempertahankan Kualitas Semen Kambing Peranakan Ettawa. Prosiding Seminar Nasional Peternakan 3. Universitas Hasanuddin. Makassar, 18 September 2017. Hlm. 64-69.
Nabilla, A., Arifiantini, R.I. and Purwantara, B., (2018). Kualitas semen segar sapi Bali umur produktif dan non-produktif serta penentuan konsentrasi krioprotektan dalam pengencer tris kuning telur. Jurnal Veteriner, 19(2), pp.242-250.
Naijian, H.R., Kohram, H., Shahneh, A.Z., Sharafi, M. and Bucak, M.N., (2013). Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze–thaw process. Cryobiology, 66(2), pp.151-155. DOI: https://doi.org/10.1016/j.cryobiol.2012.12.010
Novita, R., Karyono, T. and Rasminah, R. (2019). Kualitas Semen Sapi Brahman pada Persentase Tris Kuning Telur yang Berbeda. Jurnal Sain Peternakan Indonesia, 14(4), pp.351-358. DOI: https://doi.org/10.31186/jspi.id.14.4.351-358
Nugraha, C.D., Herwijanti, E., Novianti, I., Furqon, A., Septian, W.A., Busono, W. and Suyadi, S. (2019). Correlations between age of Bali bull and semen production at National Artificial Insemination Center, Singosari-Indonesia. J. Indones. Trop. Anim. Agric, 44, pp.258-260. DOI: https://doi.org/10.14710/jitaa.44.3.258-265
Nugraheni, T., Astirin, O.P. and Widiyani, T., (2003). Pengaruh vitamin c terhadap perbaikan spermatogenesis dan kualitas spermatozoa mencit (Mus musculus L.) setelah pemberian ekstrak tembakau (Nicotiana tabacum L.). Biofarmasi, 1(1), pp.13-19.
Parera, F., Prihatiny, Z., Souhoka, D.F. and Rizal, M., (2009). Pemanfaatan sari wortel sebagai pengencer alternatif spermatozoa epididimis sapi Bali. J. Indon. Trop. Anim. Agric, 34(1), pp.50-56. DOI: http://dx.doi.org/10.33772/jitro.v9i2.22616
Prajnanta, F., (2008). Melon: Pemeliharaan Secara Intensif dan Kiat Su ses Beragribisnis. Jakarta: Penebar Swadaya. hal, pp.8-12.
Prastowo, S., Dharmawan, P., Nugroho, T., Bachtiar, A. and Pramono, A., (2018). Kualitas semen segar sapi Bali (Bos javanicus) pada kelompok umur yang berbeda. Jurnal Ilmu Ternak Universitas Padjadjaran, 18(1), pp.1-7.
Rahayu, S., Susilawati, T. and Soewondo, A., (2020). Biologi Reproduksi: Kajian Seluler dan Molekuler. Universitas Brawijaya Press.
Rahmah, U.I.L., Imanudin, O. and Permadi, D.I.D.I., (2018). Analisis faktor-faktor yang berhubungan dengan tingkat keberhasilan inseminasi buatan pada kambing kacang (Capra hircus). Jurnal Ilmu Pertanian dan Peternakan, 6(2), pp.180-189.
Rahmiati, R., Eriani, K. and Dasrul, D., (2018). Kualitas dan morfologi abnormal spermatozoa sapi aceh pada berbagai frekuensi ejakulasi. In Prosiding Seminar Nasional Biotik (Vol. 3, No. 1). DOI: http://dx.doi.org/10.22373/pbio.v3i1.2716
Setyani, N.M., N.P. Sariani, & I.L. Oka. (2017). Heterogenitas kuantitas dan kualitas semen sapi Bali pejantan di Unit Pelaksana Teknis Balai Inseminasi Buatan Daerah, Baturiti. Peternakan Tropika, 5(1):91-104. URL: https://ojs.unud.ac.id/index.php/tropika/article/view/29466
Siswanto, I. (2010). Meningkatkan Kadar Gula Buah Melon. MT. Dosen Program Studi Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur ISBN, pp.978-602.
Sisy, D., El-Nattat, W.S., El-Sheshtawy, R.I. and El-Maaty, A.M.A., (2016). Substitution of egg yolk with different concentrations of soybean lecithin in tris-based extender during bulls' semen preservability. Asian Pacific Journal of Reproduction, 5(4). DOI: 10.1016/j.apjr.2016.10.011
Soi, M.N.J., (2016). Uji viabilitas spermatozoa sapi Bali jantan dengan menggunakan larutan natrium clorida (NACL) yang berbeda level. JAS, 1(2), pp.28-29. DOI: https://doi.org/10.32938/ja.v1i02.39
Solihati, N. and Kune, P. (2009). Pengaruh jenis pengencer terhadap motilitas dan daya tahan hidup spermatozoa semen cair sapi Simmental. Jurnal Fakultas Peternakan Universitas Padjajaran, Bandung.
Sukmawati, E., Arifiantini, R.I. and Purwantara, B. (2014). Freezing capacity of sperm on various type of superior bulls. Jurnal Ilmu Ternak dan Veteriner, 19(3), pp.168-175. URL: https://www.cabdirect.org/cabdirect/abstract/20153070689
Sulistyowati, D., Faris, M.A., Yekti, A.P.A., Wahjuningsih, S. and Susilawati, T., (2018). Kualitas semen cair sapi Peranakan Ongole pada pengencer tris aminomethan kuning telur tanpa raffinosa yang disimpan pada media yang berbeda suhu. Ternak Tropika Journal of Tropical Animal Production, 19(1), pp.38-45. DOI: https://doi.org/10.21776/ub.jtapro.2018.019.01.6
Sunarti, T.S. and Nafiu, L.O., (2016). Karakteristik spermatozoa sapi Bali setelah sexing menggunakan metode kolom albumin dengan lama waktu sexing yang berbeda. Fakultas Peternakan UHO, Kendari. Jitro, 1(1), pp.65-76.
Susilawati, T. and Yekti, A.P.A., 2018. Teknologi Inseminasi Buatan Menggunakan Semen Cair (Liquid Semen): Solusi untuk Daerah yang Tidak Ada/Sulit Nitrogen Cair. Universitas Brawijaya Press.
Susilawati, T., Isnaini, N., Satria, A.T., Huda, A.N. and Yekti, A.P.A., (2020). The pregnancy evaluation on Ongole crossbred cows by using liquid semen and frozen semen. In IOP Conference Series: Earth and Environmental Science (Vol. 478, No. 1, p. 012015). IOP Publishing. DOI: 10.1088/1755-1315/478/1/012015
Suyadi, A.R. and Iswanto, N. (2012). Pengaruh α-tocopherol yang berbeda dalam pengencer dasar tris aminomethane kuning telur terhadap kualitas semen kambing boer yang disimpan pada suhu 5oC. Jurnal Ilmiah Ilmu-ilmu Peternakan, 22(3), pp.1-8.
Tamboza, B. (2008). Serba-serbi Bertanam Melon. Bandung: Rawansah.
Tarig, A.A., Wahid, H., Rosnina, Y., Yimer, N., Goh, Y.M., Baiee, F.H., Khumran, A.M., Salman, H., Assi, M.A. and Ebrahimi, M., (2017). Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen. Veterinary world, 10(6), p.672. URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5499086/
Wahyuningsih, A. and Saleh, D.M., Sugiyatno. (2013). Pengaruh umur pejantan dan frekuensi penampungan terhadap volume dan motilitas semen segar sapi Simmental di Balai Inseminasi Buatan Lembang. Jurnal Ilmiah Peternakan, 1(3), pp.947-953.
Watson, P.F., (2000). The causes of reduced fertility with cryopreserved semen. Animal reproduction science, 60, pp.481-492.
Yahaq, M.A., Ondho, Y.S. and Sutiyono, B., (2019). Pengaruh Penambahan Vitamin C dalam Pengencer Semen Sapi Limousin yang Dibekukan Terhadap Kualitas Post Thawing. Jurnal Sain Peternakan Indonesia, 14(4), pp.380-386. DOI: https://doi.org/10.31186/jspi.id.14.4.380-386
Yendraliza, M.M., Elviriadi, Z. and Rodiallah, M., (2018). Viabilitasspermatozoa sapi Bali menggunakan pengencer andromed dengan penambahan konsentrasi sari wortel yang berbeda. Jurnal Ilmu dan Teknologi Peternakan Tropis, 6(2), pp.239-245. URL: https://garuda.kemdikbud.go.id/documents/detail/1053829
License
Copyright (c) 2023 Mujahidurrohman Mujahidurrohman, Enny Yuliani, Lukman HY
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.