Protease Enzyme Activity of Fungal Isolates from Avocado and Coconut Fleshes on Different pH and Temperature

Prapti  Sedijani; Dinda Propita Lestari; Dewa Ayu Citra Rasmi; Kusmiyati Kusmiyati

doi:10.29303/jbt.v23i4.5603

Protease Enzyme Activity of Fungal Isolates from Avocado and Coconut Fleshes on Different pH and Temperature

Authors

Prapti Sedijani , Dinda Propita Lestari , Dewa Ayu Citra Rasmi , Kusmiyati Kusmiyati

DOI:

10.29303/jbt.v23i4.5603

Published:

2023-09-01

Issue:

Vol. 23 No. 4 (2023): October - December

Keywords:

Avocado, coconut, pH, protease activity, temperature.

Articles

Downloads

How to Cite

Sedijani, P. ., Lestari, D. P., Rasmi, D. A. C., & Kusmiyati, K. (2023). Protease Enzyme Activity of Fungal Isolates from Avocado and Coconut Fleshes on Different pH and Temperature. Jurnal Biologi Tropis, 23(4), 218–225. https://doi.org/10.29303/jbt.v23i4.5603

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Protease enzymes are widely applied in the industrial field. Protease enzymes have high economic prospects with unmet demand. This article describes protease activity of two fungi isolated from avocados and from coconuts fleshes that previously have shown a high lipolytic activity on pH 7 to pH 10. The protease test was performed on PDA medium supplemented with 1% of skim milk, and adjusted to pH 7, pH 8 and pH 9 and was incubated at room temperature and at 30°C. Protease activity was determined based on the clear zone around the colony observed on day two – day eight. The results showed that variations in pH and temperature had a significant effect on the protease activity. The highest protease activity of isolate from avocado and from coconut fleshes were observed at pH 7, room temperature and incubated for 24 hours, with a protease activity index of 0.36 and 0.31 respectively.

References

Agustien, D. P., Patidar, T., Banerjee. & Patil. (2004). Production of Alkaline Protease by Penicillium sp. Under SSF conditions and Its Application to Soy Protein Hydrolysis. Process Biochemistry, 39(8) : 977-981. DOI : https://doi.org/10.1016/S0032-9592(03)00212-7.

Fuad, A.M., Rahmawati. & Mubarik. N.R. (2004). Produksi dan Karakterisasi Parsial Protease Alkali Termostabil bacillus thermoglucosidasius AF-01. Journal Mikrobiology Indonesia, 9(1) : 29-35. ISSN : 0852-358X.

Kalisz, H.M. (1998). Microbial Proteinases. Biochem. English : Biotechnol.

Li,J.Y., Ya.H.C. & Shann.T.J. (2013). Purificationand and Characterization of Acidic Protease from Aspergillus oryzae BCRC 20118. Journal Marine science and technology, 21(1) : 105-110. DOI : 10.6119/JMST-012-0529-1.

Ningsih, H., Evan, P.R., Dwiwiyati, N.S., Miranda, F.s., Resti, F., Widya, L., Abdus, S.J., Ria, P. & Elika, J. (2021). Pengantar Bioteknologi. Medan : Yayasan Kita Menulis.

Outtrup, H. dan Boyce, C.O.L. (1990). Microbial Proteinases and Biotechhnology. In : Fogarty C.T, Kelly K. (eds). Microbial Enzymes and Biotechnology. London ; Elssevier.

Poernomo, A.T., Isnael. & Purwanto. (2015). Aktivitas Invitro Enzim Fibrinolitik Ekstrak Tempe hasil Fermentasi Rhizopus Oligosporus ATCC 6010 pada Substrat Kedelai Hitam. Berkala Ilmiah Kimia Farmasi, 4(2) : 18-24. ISSN : 2302-8270. URL: https://journal.unair.ac.id/download-fullpapers-bikf2b8862565b2full.pdf.

Ratnayani, K., M.Nasib., J. Sibrani. & A.A.L.AM. Laksmiwati. (2018). Aktivitas Protease pada Getah Bagian Batang dari Tiga Jenis Spesies Tanaman Kamboja (Plumeria L). Jurnal Kimia, 12(2) : 147-151. DOI : https://doi.org/10.24843/JCHEM.2018.v12.i02.p09.

Rosa, E., Ekowati, C.N., Handayani, T., Ikhsanuddin, A., Apriliani, F. & Arifyanto, A. (2020). Characterization of Entomopathogenic Fungi as a Natural Biological Control of American Cokroches . Periplaneta Americana. 21(11). 44-52.DOI : https://doi.org/10.13057/biodiv/d211131.

Sabrini, Z., Isworo, R. & Rejeki, S.F. (2021). Aktivitas Enzimatis Biakan Kapang Aspergillus Section Nigri (Diponegoro University Culture Collection) dan Identifikasi Molekuler Isolat Potensial, Bioma. 23(1) : 1-5. DOI : https://doi.org/10.14710/bioma.23.1.

Sedijani, P., Dewa, A.C.R., Kusmiyati., dan Riska, A.A. (2021). Aktivitas Lipolitik Kuat Jamur Isolasi Daging Kelapa dan Alpukat pada pH dan Suhu Berbeda. Jurnal Penelitian Pendidikan IPA, 7 : 365-369. DOI : 10.29303/jppipa.v7iSpecialIssue.1261.

Suhartono,M.C.L. (2000). Exploration Of Indonesian Thermophiles Producing Thermostable Chitinolytic Enzymes. Bogor : Bogor Agriculture University.

Sumardi., Vanya. Q., Christina, N.E., Salman, F. & Acmad, F. (2021). Aktivitas Enzim Hidrolase pada Penapisan Isolat Actinomycetes Kandidat Probiotik Udang. Jurnal biologi dan Pembelajaran Biologi, 6(1) : 24-36. DOI : 10.32528/bioma.v6il. 3548.

Sumarlin,L.O. (2008). Aktivitas Protease dari Bacillus circulans pada Media Pertumbuhan dengan pH Tidak Terkontrol, Jurnal Kimia Valensi, 1(2) : 58-62. DoI : 10.15408.jkv.v1i2.215.

Susanti, Elfi. (2002). Isolasi dan Karakterisasi Protease dari Bacillus Subtilis 1012M15. Biodiversitas. 4(1). DOI : 10.13057/biodi/d040103.

Syafie, Yusron. (2014). Efektivitas Enzim Protease Aspergillus sp. pada Proses Unhairing terhadap Histologi Kulit Samak. Jurnal Bioedukasi, 391) : 313-317. DOI : https://doi.org/10.33387/bioedu.v2i1.67.

Yusriah & Nengah.D.K. (2013). Pengaruh pH dan Suhu Terhadap Aktivitas Protease Penicillium sp. Jurnal Sains dan Seni Pomits, 2(1) : 48-50. DOI : 10.12962/j23373520.v2i1.2744

Yusron,A., Nadila P. & Hamid A. (2021). Uji Aktivitas Enzim Protease pada Kedelai Grade C yang difermentasi Padat dengan Inokulum Tempe Kediri. Simposium Nasional Rapi, 10(10) : 235-242. ISSN : 2686-4274. URL: https://proceedings.ums.ac.id/index.php/rapi/article/view/166/166

Zainuddin, M. (2017). Isolasi, Seleksi dan Identifkasi Genotipik 16 S-rRNA Bakteri Proteolitik Indogeneus dari Ekosistem Mangrove Karimunjawa Sebagai Kandidat Konsursium Probiotik untuk Bioremediasi Limbah Organik Tambak. Akuatik Jurnal Sumberdaya Perairan. 11(1) : 71-77. ISSN : 1978-1652. URL: https://journal.ubb.ac.id/index.php/akuatik/article/view/218.

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

Most read articles by the same author(s)

Lalu Zulkifli, Prapti Sedijani, Dewa Ayu Citra Rasmi, Lalu Wira Zain Amrullah, Screening and Molecular Identification of Phosphate-Solubilizing Rhizobacteria from Mangrove Ecosystem of the Lombok Island , Jurnal Biologi Tropis: Vol. 20 No. 3 (2020): September - Desember
Prapti Sedijani, Dewa Ayu Citra Rasmi, Kusmiyati Kusmiyati, Miko Eniarti, Siti Rohimah, Isolation and Activity Test of Lipolitic Bacteria on Different pH and Temperature , Jurnal Biologi Tropis: Vol. 22 No. 3 (2022): July - September
Rizal Umami, Lalu Zulkifli, Syamsul Bahri, Mahrus, Prapti Sedijani, Molecular Characterization of Neem Rhizosphere Bacteria as A Nitrogen Fixer Isolated from The Dry Land of East Lombok , Jurnal Biologi Tropis: Vol. 23 No. 2 (2023): Special Issue
Baiq Elita Mandalini, Prapti Sedijani, Ahmad Raksun, The Effects of Liquid Organic Fertilizer of Vegetable Waste on The Growth of Cayenne Pepper Plants (Capsicum frutescens L.) , Jurnal Biologi Tropis: Vol. 22 No. 4 (2022): October - December
Priyo Hartanto, Prapti Sedijani, Lalu Zulkifli, Miko Erniarti, The effect of Lemongrass (Cymbopogon nardus) Extract in inhibiting Bread Fungal Growth, Aspergilus Oyizae Using a combination of N-Hexane-Ethanol Solvent , Jurnal Biologi Tropis: Vol. 22 No. 1 (2022): January - March
Prapti Sedijani, Bq. Novi Aprilia, Dewa Ayu Citra Rasmi, Kusmiyati Kusmiyati, Isolation and Screening of Amilolytic Bacteria Isolate from Cassava (Manihot utilissima) , Jurnal Biologi Tropis: Vol. 23 No. 4 (2023): October - December
Valeria Gifridus, Prapti Sedijani, Ahmad Raksun, Effect of Silver Black Plastic Mulch and Organic Fertilizer Dosage on Plant Chlorophyll Growth and Content Cayenne Pepper (Capsicum annum L.) , Jurnal Biologi Tropis: Vol. 24 No. 1 (2024): Januari - Maret
Prapti Sedijani, Nida'an Khovia , Dewa Ayu Citra Rasmi, Kusmiyati, Fungal Crude Lipase Enzyme Produced Using the SSF (Solid State Fermentation) Method Increases The Washing Test Performance , Jurnal Biologi Tropis: Vol. 23 No. 1 (2023): Special Issue
Masaradi Putra, Ahmad Raksun, Prapti Sedijani, Effect of NPK Fertilizer and Vermicompost on The Vegetative Growth of Green Eggplant (Solanum melongena L.) , Jurnal Biologi Tropis: Vol. 25 No. 1 (2025): Januari - Maret
Baiq Fadilla Yuliana, Lalu Zulkifli, Dewa Ayu Citra Rasmi, Prapti Sedijani, Isolation and Identification of IAA-Producing and Phosphate-Solubilizing Rhizobacteria from Sesuvium portulacastrum and In Vitro Growth-Promotion Effect on Vigna radiata L. , Jurnal Biologi Tropis: Vol. 25 No. 4a (2025): Special Issue

<< < 1 2 3 4 5 6 7 > >>

Protease Enzyme Activity of Fungal Isolates from Avocado and Coconut Fleshes on Different pH and Temperature

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow