Potensi Aktivitas Antibakteri Minuman Fungsional Kombucha Berbahan Dasar Bunga Kecombrang (Etlingera elatior) Berdasarkan Lamanya Waktu Fermentasi
Authors
Putu Rima Sintyadewi , Pande P Elza Fitriani , Ida Ayu Putu Ary Widnyani , Putu IndrayoniDOI:
10.29303/jbt.v23i1.6126Published:
2023-12-06Issue:
Vol. 23 No. 1 (2023): Special IssueKeywords:
Antibacterial, functional drink, kecombrang flower, probiotic.Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
According to same reports, kombucha is a functional beverage that has the ability to boost immune system function by increasing T cells and containing antimicrobial substances that guard against microbial illness. The antibacterial properties of kombucha derived from kecombrang flowers have not been examined in a any of the numerous studies conducted on the beverage’s antimicrobial activity. The purpose of this study is to investigate how the lengt of fermentation effects the kecombrang flower kombucha antibacterial effectiveness. Fermentation times for the treatments were as follows (P1) 0 days, (P2) 3 days, (P3) 6 days, and (P4) 9 days. The commensal pathogenic microorganisms Escherichia coli, Salmonella typhimurium and Stapylococcus aureus were employed in this study. According to the research findings, the antibacterial activity of kecombrang flower kombucha was significantly impacted (P<0,01) by the duration of fermentation. The maximun antibacterial activity was found in kecombrang flower kombucha that was fermented for nine days (P4). It had an inhibitory power of 12,0 mm against Escherichia coli, 11,5 mm against Salmonella typhimurium, and 14,5 mm against Staphylococcus aureus. Kecombrang flower kombucha is known for its broad spectrum of antibacterial activity, which can suppress the growth of both gram-positif and gram-negatif pathogenic bateria. Based on these results, kecombrang flower kombucha has the potential to be an antibacterial agent and can functions as an alternative approach to functional drinks to control commensal pathogenic bacteria.
References
Alizadeh, A.M., Hashempour-Baltork, F., Alizadeh-Sani, M., Maleki, M., Azizi-Lalabad, M. & Khosravi-Darani, K. (2020). Inhibition of Clostridium botulinum and its toxins by probiotic bacteria and their metabolites: an update review. Quality Assurance and Safety of Crops & Foods, 12: 59–68. Doi: https://doi.org/10.15586/qas.v12iSP1.823
Battikh, K., Chaeib, A. Bakhrouf & E. Ammar. (2013). Antibacterial and Antifungal Activities of Black and Green Kombucha Teas. Journal of Food Biochemistry, 37(2): 231-236. Doi: https://doi.org/10.1111/j.1745-4514.2011.00629.x
Bhattacharya, D., Bhattacharya, S., Patra, M.M., Chakravorty, S., Sarkar, S., Chakraborty, W., et al., (2016). Antibacterial activity of polyphenolic fraction of kombucha against enteric bacte- rial pathogens. Current Microbiology 73: 885–896. Doi: https://doi. org/10.1007/s00284-016-1136-3
Bellassoued, K., Ferdaws, G., Fatma, M.A. Josh, V.P., Abdelfattah, E., & Emna, A. (2015). Protective Effect of Kombucha on Rats Fed A Hypercholesterolemic Diet is Mediated by its Antioxidant Activity. Journal Pharmaceutical Biology, 53(11): 199-1709. Doi: https://doi.org/10.3109/13880209.2014.1001408
Bermudez-Brito, M., Plaza-Diaz, J., Munoz-Quezada, S., Gomez-Llorente, C., & Gil, A. (2012). Probiotic mechanisms of action. Ann. Nutr. Metab, 61: 160–174. Doi: https://doi.org/10.1159/000342079
Bhattacharya, S., Gachhui, R., & Sill, P.C. (2013). Effect of Kombucha, a fermented black tea in attenuating oxidative stress mediated tissue demage in alloxan induced diabetic rats. Food and Chemical Toxicology, 60: 328-340. Doi: https://doi.org/10.1016/j.fct.2013.07.051
Bhattacharya, D., Bhattacharya, S., Patra, M. M., Chakravorty, S., Sarkar, S., Chakraborty, W., Koley H., … & Gachhui, R. (2016). Antibacterial activity of polyphenolic fraction of kombucha against enteric bacterial pathogens. Current Microbiology, 73:885–896. Doi: https://doi.org/10.1007/s00284-016-1136-3
Enan, G., Abdel-Shafi, S., Abdel-Haliem, M.F & Negm, S. (2013). Characterization of probiotic lactic acid bacteria to be used as starter and protective cultures for dairy fermentations. Int. J. Probiotics Prebiotics, 8:157–163.
Gao, Z.H., Daliri, E.B.M., Wang, J., Liu, D.H., & Chen, S.G.(2019). Inhibitory effect of lactic acid bacteria on foodborne pathogens: a review. Journal of Food Protection, 82: 441– 453. Doi: https://doi.org/10.4315/0362-028X.JFP-18-303
Harmita & M. Radji (2008). Kepekaan Antibiotik dalam Buku Ajar Analisis Hayati Ed. 3, EGC, Jakarta, pp. 1- 5, 2008
Ilicic, M., Kanuric, K., Milanovic, S., Loncar, E. S., Djuric, M. S., & Malbasa, R. V. (2012). Lactose fermentation by Kombucha-a process to obtain new milk-based beverages. Romanian Biotechnological Letters, 17(1), 7013-7021.
Ismaiel, A.A., Ali, A.E.-S & Enan, G. (2014). Incidence of Listeria in Egyptian meat and dairy samples. Food Sci. Biotechnol, 23: 179–185. Doi: https://doi.org/10.1007/s10068-014-0024-5
Kadyan, S., Rashmi, H.M., Pradhan, D., Kumari, A., Chaudhari, A & Deshwal, G.K. (2021). Effect of lactic acid bacteria and yeast fermentation on antimicrobial, antioxidative and metabolomic profile of naturally carbonated probiotic whey drink. LWT Food Science and Technology, 142: 111059. Doi: https://doi.org/10.1016/j.lwt.2021.111059
Kaewkod, T., Bovonsombut, S. & Tragoolpua, Y. (2019). Efficacy of kombucha obtained from green, oolong, and black teas on inhibition of pathogenic bacteria, antioxidation, and toxicity on colorectal cancer cell line. Microorganisms, 7: 700. Doi: https://doi.org/10.3390/microorganisms7120700
Kallel L, Desseaux V, & Hamdi M. (2012). Insights into the fermentation biochemistry of Kombucha teas and potential impacts of Kombucha drinking on starch digestion. Food Res Int 49:226–32, Doi: http://dx.doi.org/10.1016/j.foodres.2012.08.018
Kumar V & V.K. Joshi. (2016). Kombucha: Technology Microbiology, Production, Composition and Therapeutic Value. Intl. J. Food. Ferment. Technol, 6(1), 13-24. Doi: http://dx.doi.org/10.5958/2277-9396.2016.00022.2
Kohanski, M.A., D.J. Dwyer & J.J. Collins. (2010). How antibiotics kill bacteria: from targets to networks. Nat Rev Microbiol, 8 (6): 423–435. Doi: https://doi.org/10.1038%2Fnrmicro2333
Nurhayati,L.S., Nadhira, Y & Akhmad, H. 2020. Perbandingan Pengujian Aktivitas Antibakteri Stater Yougurt Dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2):41-46. Doi: https://doi.org/10.24198/jthp.v1i2.27537
Osman, A., El-Didamony, G., Sitohy, M., Khalifa, M & Enan, G. (2016). Soybean glycinin basic subunit inhibits methicillin resistant vancomycin intermediate Staphylococcus aureus (MRSA-VISA) in vitro. Int. J. Appl. Res. Nat. Prod. 9: 17–26. https://www.researchgate.net/publication/298413768_Soybean_glycinin_basic_subunit_inhibits_methicillin_resistant-vancomycin_intermediate_Staphylococcus_aureus_MRSA-VISA_in_vitro
Osman, A., Bin-Jumah, M., Abd El-Hack, M., Elaraby, G., Swelum, A.A., Taha, A.E., Sitohy, M., Allam, A.A & Ashour, E.A. (2020). Dietary supplementation of soybean glycinin can alter the growth, carcases traits, blood biochemical indices, and meat quality of broilers. Poult. Sci, 99:820–828. Doi: https://doi.org/10.1016/j.psj.2019.12.026
Poolman, J.T & Anderson, A.S. (2018). Escherichia coli and Staphylococcus aureus: Leading bacterial pathogens of healthcare associated infections and bacteremia in older-Age populations. Expert Rev. Vaccines, 17:607–618. Doi: https://doi.org/10.1080/14760584.2018.1488590
Ruiz, B., A. Chavez, A. Forero, Y. GarcíaHuante, A. Romero, & M. Sánchez (2010). Production of microbial secondary metabolites: regulation by the carbon source. Crit. Rev. Microbiol, 36: 146–167. Doi: https://doi.org/10.3109/10408410903489576
Simons, A., Alhanout, K. & Duval, R.E. (2020). Bacteriocins, antimicrobial peptides from bacterial origin: overview of their biology and their impact against multidrug-resistant bacteria. Microorganisms 8: 639. Doi: http://doi.org/10.3390/ microorganisms8050639
Sreeramulu, G., Zhu, Y. & Knol, W. (2000). Kombucha fermentation and its antimicrobial activity. Journal of Agricultural and Food Chemistry, 48: 2589–2594. Doi: http://doi.org/10.1021/jf991333m
Tan, W.C., Muhialdin, B.J, & Hussin, A.S.M., (2020). Influence of storage conditions on the quality, metabolites, and biological activity of Soursop (Annona muricata. L.) kombucha. Frontiers in Microbiology, 11: 603481. Doi: http://doi.org/10.3389/fmicb.2020.603481
Vitas, J., Malbasa, R., Grahovac, J., & Loncar, E. (2013). The antioxidant activity of Kombucha fermented milk products with stinging nettle and winter savory. Chemical Industry and Chemical Engineering Quarterly, 19(1):129–139. Doi: https://doi.org/10.1016/j
Verrillo, M., Salzano, M., Cozzolino, V., Spaccini, R. & Piccolo, A., 2021. Bioactivity and antimicrobial properties of chemically characterized compost teas from different green composts. Waste Management 120: 98–107. Doi: http://doi.org/10.1016/j. wasman.2020.11.013
Velićanski, A., Cvetković, D.D., Markov, S.L., Šaponjac, V.T.T. & Vulić, J.J., (2014). Antioxidant and antibacterial activity of the beverage obtained by fermentation of sweetened lemon balm (Melissaofficinalis L.) tea with symbiotic consortium of bacteria and yeast. Food Technology and Biotechnology, 52: 420–429. Doi: http://doi.org/10.17113/b.52.04.14.3611
Vorha, B.M., Shazrul, F., Fareed S., & Othman, B.A. (2019). Effects of Medium Variation and Fermentation Time on the Antiokxidant and Antimicrobial Properties of Kombucha. Malaysian Journal of Fundamental and Applied Sciences. 298-302. Doi: https://doi.org/10.11113/mjfas.v15n2-1.1536
License
Copyright (c) 2023 Putu Rima Sintyadewi, Pande P Elza Fitriani, Ida Ayu Putu Ary Widnyani, Putu Indrayoni
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.