Determination of Total Flavonoid Content and Antioxidant Activity of Avocado Stem Bark (Persea americana Mill) Ethanol Extract in Inhibiting DPPH

Richa Poetri Risnata; Agriana Rosmalina Hidayati

doi:10.29303/jbt.v24i4.7776

Determination of Total Flavonoid Content and Antioxidant Activity of Avocado Stem Bark (Persea americana Mill) Ethanol Extract in Inhibiting DPPH

Authors

Richa Poetri Risnata , Agriana Rosmalina Hidayati

DOI:

10.29303/jbt.v24i4.7776

Published:

2024-11-09

Issue:

Vol. 24 No. 4 (2024): Oktober - Desember

Keywords:

Antioxidants, avocado stem bark, DPPH, total flavonoids content.

Articles

Downloads

pdf

How to Cite

Risnata, R. P., & Hidayati, A. R. (2024). Determination of Total Flavonoid Content and Antioxidant Activity of Avocado Stem Bark (Persea americana Mill) Ethanol Extract in Inhibiting DPPH . Jurnal Biologi Tropis, 24(4), 877–886. https://doi.org/10.29303/jbt.v24i4.7776

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Excessive free radicals in the body can trigger degenerative diseases. Therefore, antioxidant help is needed from outside the body to inhibit free radicals. Long-term use of synthetic antioxidants is limited because they can cause cancer. This encourages the development of natural antioxidants, one of which comes from avocado stem bark. Avocado stem bark contains flavonoids which act as antioxidants. This study aims to determine the total flavonoid content and antioxidant activity of avocado stem bark ethanol extract in inhibiting DPPH. Avocado stem bark was extracted using sonication followed by the identification of flavonoids using a tube test and thin layer chromatography test. Determination of total flavonoid content was carried out using the colorimetric method and antioxidant testing using the DPPH method. The results obtained were that the avocado stem bark was positive for containing flavonoids based on tube tests and thin layer chromatography. The results of determining the flavonoid content in the ethanol extract of avocado stem bark were 5.572 mgQE/g. The ethanol extract of avocado stem bark is a very strong antioxidant with an IC₅₀ of 21.221 ppm. So it was concluded that avocado stem bark can be used as a natural antioxidant candidate.

References

Amalia, B. R., Muliasari, H., & Hidayati, A. R. (2023). Uji Aktivitas Antioksidan Kombinasi Ekstrak Kulit Pisang Kepok (Musa paradisiaca L.) dan Kulit Buah Naga Merah (Hylocereus polyrhizus (Weber) Britton & Rose) dengan Metode DPPH. Jurnal Pharmascience, 10(1), 69. https://doi.org/10.20527/jps.v10i1.14863

Aminah, A., Tomayahu, N., & Abidin, Z. (2017). Penetapan Kadar Flavonoid Total Ekstrak Etanol Kulit Buah Alpukat (Persea americana Mill.) dengan Metode Spektrofotometri Uv-Vis. Jurnal Fitofarmaka Indonesia, 4(2), 226–230. https://doi.org/10.33096/jffi.v4i2.265

Anal, A. K., Jaisanti, S., & Noomhorm, A. (2014). Enhanced yield of phenolic extracts from banana peels (Musa acuminata Colla AAA) and cinnamon barks (Cinnamomum varum) and their antioxidative potentials in fish oil. Journal of Food Science and Technology, 51(10), 2632–2639. https:// doi.org/10.1007/s13197-012-0793-x

Ariviani, S., & Rajendra, F. (2021). Kacang Tunggak Sebagai Pangan Sumber Antioksidan Potensial dan Alternatif Strategi Peningkatan Kapasitas Antioksidatifnya. Yogyakarta: Deepublish.

Asmorowati, H., & Lindawati, N. Y. (2019). Penetapan kadar flavonoid total alpukat (Persea americana Mill.) dengan metode spektrofotometri. Jurnal Ilmiah Farmasi, 15(2), 51–63.

Azizah, D. N., Kumolowati, E., & Faramayuda, F. (2014). Penetapan Kadar Flavonoid Metode AlCl3 Pada Ekstrak Metanol Kulit Buah Kakao (Theobroma cacao L.). Kartika Jurnal Ilmiah Farmasi, 2(2), 45–49. https://doi.org/10.26874/kjif.v2i2.14

Bertin, J., Bindzi, A. Z., Ngono, R., Abondo, M., Ngoupayo, J., & Victorine, B. F. (2020). Micrography, phyto-chemical screening and physico-chemical properties of Bridelia micrantha (Hochst.) Baill. and Persea americana L. stem bark. Journal of Pharmacognosy and Phytochemistry, 9(3). www.phytojournal.com

Departemen kesehatan republik indonesia. (1985). Cara Pembuatan Simplisia. Ditjen POM RI.

Dewatisari, W. F., Rumiyanti, L., & Rakhmawati, I. (2018). Rendemen dan Skrining Fitokimia pada Ekstrak Daun Sanseviera sp. Jurnal Penelitian Pertanian Terapan, 17(3), 197. https://doi.org/ 10.25181/jppt.v17i3.336

Dewi, A. P. (2019). Penetapan Kadar Vitamin C Dengan Spektrofotometri Uv-Vis Pada Berbagai Variasi Buah Tomat. JOPS (Journal Of Pharmacy and Science), 2(1), 9–13. https://doi.org/10.36341/jops. v2i1.1015

Dewi, I. S., Septawati, T., & Rachma, F. A. (2021). Skrining Fitokimia Ekstrak Etanol Kulit dan Biji Terong Belanda ( Solanum betaceum Cav.). Prosiding Seminar Nasional UNIMUS, 4, 1210–1218.

Dewi, S. R., Argo, B. D., & Ulya, N. (2018). Kandungan Flavonoid dan Aktivitas Antioksidan Ekstrak Pleurotus ostreatus. Rona Teknik Pertanian, 11(1), 1–10. https://doi.org/10.17969/rtp.v11i1.9571

Ergina, S. N. (2014). Ergina, Siti Nuryanti & Indarini Dwi Pursitasari. Uji Kualitatif Senyawa Metabolit Sekunder Pada Daun Palado (Agave angustifolia) Yang Diekstraksi Dengan Pelarut Air Dan Etanol. Akad. Kim, 3(3), 165–172.

Erlidawati., & S. (2018). Potensi Antioksidan Sebagai Antidiabetes. Syiah Kuala University Press.

Fauziah, A., Sudirga, S. K., & Parwanayoni, N. M. S. (2021). Uji Antioksidan Ekstrak Daun Tanaman Leunca (Solanum nigrum L.). Metamorfosa: Journal of Biological Sciences, 8(1), 28. https://doi.org/ 10.24843/metamorfosa.2021.v08.i01.p03

Forestryana, D., & Arnida, A. (2020). Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Daun Jeruju (Hydrolea Spinosa L.). Jurnal Ilmiah Farmako Bahari, 11(2), 113. https://doi.org/10. 52434/jfb.v11i2.859

Harningsih, T., & Thalitha Larassati Sekolah Tinggi Ilmu Kesehatan Nasional, W. (2020). Uji Aktivitas Antioksidan Dan Tabir Surya Kombinasi Ekstrak Kulit Buah Pisang Kepok (Musa Paradisiaca Linn) Dan Ekstrak Kulit Buah Alpukat (Persea americana Mill). Jurnal.Akfarsam.Ac.Id, 6(2), 231–239. https://www.jurnal.akfar sam.ac.id/index.php/jim_akfarsam/article/view/365

Husa, F., & Mita, S. R. (2020). Identifikasi Bahan Kimia Obat dalam Obat Tradisional Stamina Pria dengan Metode Kromatografi Lapis Tipis. Farmaka, 18(2), 16–25. https://jurnal.unpad.ac.id/farmaka/article/view/25955

Indonesia, departemen K. R. (2017). Farmakope Herbal Indonesia (edisi kedu). Ditjen POM RI.

Ipandi, I., Triyasmono, L., & Prayitno, B. (2016). Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Kajajahi (Leucosyke capitellata Wedd.). Jurnal Pharmascience, 5(1), 93–100.

Islamie, R., & Puspita. (2015). Uji Potensi Antibakteri Ekstrak Etanol Kulit Batang Alpukat (Persea americana Mill.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aeruginosa ATCC 25922. Jurnal Ilmiah Sains Dan Teknologi.

Kemit, N., Dewa, G.M.P., & Pande K.D.K. (2019). Stabilitas Senyawa Flavonoid Ekstrak Daun Alpukat (Persea americana Mill.) Terhadap Perlakuan pH dan Suhu Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology), 6(1), 34–42.

Koirewoa, Y. A., Fatimawali, & Wiyono, W. I. (2012). Isolasi Dan Identifikasi Senyawa Flavonoid Dalam Daun Beluntas (Pluchea Indica L .). Jurnal Farmasi, 47–52.

Kopon, A. M., Baunsele, A. B., & Boelan, E. G. (2020). Skrining Senyawa Metabolit Sekunder Ekstrak Metanol Biji Alpukat (Persea americana Mill.) Asal Pulau Timor. Akta Kimia Indonesia, 5(1), 43. https://doi.org/10.12962/j25493736.v5i1.6709

Latif, R. A., Mustapa, M. A., & Duengo, S. (2018). Analisis Kadar Senyawa Flavonoid Ekstrak Metanol Kulit Batang Waru (Hibiscus tiliaceus L.) dengan Menggunakan Metode Spektrofotometri UV-Vis. Seminar Nasional Farmasi Universitas Negeri Gorontalo, 435–448.

Lohita Sari, B., Rurianti, W., & Simanjuntak, P. (2015). Toksisitas, Aktivitas Antioksidan Dan Antibakteri Ekstrak Air Kulit Kayu Massoi (Cryptocarpa massoy (Lauraceae)). FITOFARMAKA: Jurnal Ilmiah Farmasi, 4(1), 18–26. https://doi.org/10.33751/ jf.v4i1.183

Mardiyaningsih, A., & Ismiyati, N. (2014). Cytotoxic Activity Of Ethanolic Extract Of Persea americana Mill. Leaves On HeLa Cervical Cancer Cell Aktivitas Sitotoksik Ekstrak Etanolik Daun Alpukat (Persea americana Mill.) Pada Sel Kanker Leher Rahim HeLa. Traditional Medicine Journal, 19(1), 2014.

Molyneux, P. (2004). The Use of the Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin Journal of Science and Technology, 26(December 2003), 211–219. https://doi.org/10.1287/isre.6.2.144

Mulyaningsih, S., Yasrifah, H. S., & Taofik, D. B. I. (2022). Uji Kadar Flavonoid Total dari Ekstrak Daun Alpukat (Persea americana Mill.). Jurnal Life Science: Jurnal Pendidikan Dan Ilmu Pengetahuan Alam, 4(2), 64–69. https://doi.org/10.31980/jls. v4i2.2352

Murwanto, P. E., & Santosa, D. (2012). Uji Aktivitas Antioksidan Tumbuhan Cynara scolimus L., Artemisia china L., Borreria repensDC., Polygala paniculata L. Hasil Koleksi dari Taman Nasional Gunung Merapi dengan Metode Penangkapan Radikal DPPH (2,2-Difenil-1- Pikrilhidra- zil). Majalah Obat Tradisional, 17(3), 53.

Muthia, R., Saputri, R., & Verawati, S. A. 2019. Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Mundar (Garcinia forbesii King.) Menggunakan Metode DPPH (2,2-Diphenyl-1-Picrylhydrazil), Jurnal Pharmascience, 6(1), 74

Mutiara, R., Priani, S. E., & Mulyanti, D. (2015). Uji Aktivitas Antioksidan Ekstrak Kulit Batang Kayu Manis (Cinnamomum burmanni Nees ex Bl.) dan Formulasinya dalam Bentuk Sediaan Masker Gel Peel Off. Prosiding Penelitian SPeSIA Unisba 2015, 5(2), 223–224. http://ejournal. poltektegal.ac.id/index.php/parapemikir/article/view/381

Osuntokun, O. temitope, MO, A., OM, A., & AO, O. (2018). Efficacy of Essential Oils from Persea americana Stem Bark and Seed Extracts. Journal of Applied Microbiology and Biochemistry, 01(03), 0–6. https:// doi.org/10.21767/2576-1412.100012

Pourmorad, Liang JY, Ho ChW, T. C. (2006). Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia). Food Chemistry 120:1,. Food Chemistry, 20(1), 290–295.

Riwanti, P., Izazih, F., & Amaliyah. (2020). Pengaruh Perbedaan Konsentrasi Etanol pada Kadar Flavonoid Total Ekstrak Etanol 50,70 dan 96%. Journal of Pharmaceutical Care Anwar Medika, 2(2), 82–95.

Rosamah, E. (2019). Kromatografi Lapis Tipis. Mulawarman University Press.

Rusmawati, L., Rahmawan Sjahid, L., & Fatmawati, S. (2021). Pengaruh Cara Pengeringan Simplisia Terhadap Kadar Fenolik Dan Aktivitas Tabir Surya Ekstrak Etanol 70% Daun Cincau Hijau (Cyclea barbata Miers.). Media Farmasi Indonesia, 16(1), 1643–1651. https://doi.org/10. 53359/mfi.v16i1.171

Sari, D. Y., R, W., & AN, T. (2021). Penentuan Kadar Flavonoid Total Ekstrak Etanol Jamur Susu Harimau (Lignosus rhinocerus). Jurnal Farmasi Udayana, 10(1), 23. https://doi.org/10.24843/jfu. 2021.v10.i01.p03

Sholihah, M., Ahmad, U., & Budiastra, I. W. (2017). Application of Ultrasonic Wave to Increase Extraction Yield and Effectiveness of Antioxidant from Mangosteen Rind. Jurnal Keteknikan Pertanian, 05(2), 1–11. https://doi.org/10.19028/jtep.05.2.161-168

Silva, Y. R. R., Silva, L. D., Rocha, T. L., Dos Santos, D. B., Bezerra, J. C. B., Machado, K. B., DE PAULA, J. A. M., & Amaral, V. C. S. (2020). Molluscicidal activity of persea americana mill. (lauraceae) stem bark ethanolic extract against the snail biomphalaria glabrata (say, 1818): A novel plant-derived molluscicide? Anais Da Academia Brasileira de Ciencias, 92(4), 1–16.https://doi.org/10.1590/00013765202020200715

Siyanti, A., Fitriani, N., & Angga. (2019). Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Alpukat (Persea americana Mill.) terhadap Peredaman DPPH. Proceeding of Mulawarman Pharmaceuticals Conferen-ces, 10, 72–75. https://doi.org/10.25026/ mpc.v10i1.357

Suharyanto, & Prima, D. A. N. (2020). Penetepan Kadar Flavonoid Total pada Daun Ubi Jalar Ungu (Ipomoea batatas L.) yang Berpotensi Sebagai Hepatoprotektor dengan Metode Spektrofotometri UV-Vis. Cendekia Journal of Pharmacy, 4(2), 110–119.

Sulistyoingdyah, F., & Ramayani, L. . (2017). Skrining Fitokimia Ekstrak Infusa Kulit Petai (Parkia speciosa Hassk). Jurnal Jawara, 5 (1).

Susiloningrum, D., & Sari, D. E. M. (2021). Uji Aktivitas Antioksidan Dan Penetapan Kadar Flavonoid Total Ekstrak Temu Mangga ( Curcuma mangga Valeton & Zijp ) Dengan Variasi Konsentrasi Pelarut. Cendekia Journal of Pharmacy, 5(2), 117–127.

Susilowati, & Estiningrum, D. (2016). Penentuan Golongan Seyawa dan Total Flavonoid Ekstrak Etanol Sarang Semut (Myrmecodia pendens Merr & Perry) secara Spektrofotometri Uv-Vis Determination. Journal of Pharmacy, 5(1), 19–24. https://ojs.stikesnas.ac.id/index.php/jf/article/view/152

Sutriningsih, & Astuti, I. W. (2017). Uji Antioksidan dan Formulasi Sediaan Masker Peel -Off dari Ekstrak Biji Alpukat (Persea americana Miil.) Dengan Perbedaan Konsentrasi PVA (Polivinil Alkohol). Indonesi Natural Research Pharma-ceutichal Journal, 1(9), 67–75.

Syarifuddin, K. A., & Dewi, A. (2022). Analisis Kadar Flavonoid Total Ekstrak Etanol Tempuyung ( Sonchus arvensis ) Dengan Menggunakan Metode Spektrofotometri UV-Vis. FitoMedicine: Journal Pharmacy and Sciences, 12(2), 69–76.

vitasari, E. . (2013). Antihiperlipidemia Ekstrak Etanol Batang Kayu Kuning (Arcangelisia flafa (L.) Merr.) terhadap Tikus Putih Galur Wistar yang Diinduksi Pakan Tinggi Lemak. Sekolah Tinggi Ilmu Farmasi Yayasan Farmasi: Semarang.

Wahid, A., Diah, M., & Rama, M. (2017). 224183-Uji-Aktivitas-Antioksidan-Ekstrak-Air-Da. 6(May), 125–131.

wahyuningrum, retno ra. (n.d.). Antioksidan Daun Teh. PT. ISFI.

Yuliantari, N. W. A., Widarta, I. W. R., & Permana, I. D. G. M. (2017). Pengaruh Suhu dan Waktu Ekstraksi Terhadap Kandungan Flavonoid dan Aktivitas Antioksidan Daun Sirsak (Annona muricata L.) Menggunakan Ultrasonik The Influence of Time and Temperature on Flavonoid Content and Antioxidant Activity of Sirsak Leaf (Annona mur. Media Ilmiah Teknologi Pangan, 4(1), 35–42.

yuliarti, N. (2009). A to Z Food Supplement. CV ANDI.

Yuslianti, E. R. (2018). Pengantar Radikal Bebas dan Antioksidan (edisi 1). Yogyakarta: Deepublish.

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

Determination of Total Flavonoid Content and Antioxidant Activity of Avocado Stem Bark (Persea americana Mill) Ethanol Extract in Inhibiting DPPH

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow