Antibacterial of Endophytic Bacteria from Papaya (Carica papaya) Seeds Againts Staphylococcus aureus and Escherichia coli

Esti Rizkiana Pratiwi; Nadiah Al Batati; Nuzzulul Anisa Firamadhani

doi:10.29303/jbt.v24i1b.8018

Antibacterial of Endophytic Bacteria from Papaya (Carica papaya) Seeds Againts Staphylococcus aureus and Escherichia coli

Authors

Esti Rizkiana Pratiwi , Nadiah Al Batati , Nuzzulul Anisa Firamadhani

DOI:

10.29303/jbt.v24i1b.8018

Published:

2024-12-12

Issue:

Vol. 24 No. 1b (2024): Special Issue

Keywords:

Antibacterial, endophytic bacteria, papaya, seeds.

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Pratiwi, E. R., Al Batati, N., & Firamadhani, N. A. (2024). Antibacterial of Endophytic Bacteria from Papaya (Carica papaya) Seeds Againts Staphylococcus aureus and Escherichia coli. Jurnal Biologi Tropis, 24(1b), 230–236. https://doi.org/10.29303/jbt.v24i1b.8018

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Abstract

Endophytic bacteria were produce antibacterial compounds from various plants has been widely reported as considered efficient in overcoming resistant pathogen infections. This study aims to obtain endophytic bacteria from papaya seeds which can produce antibacterials. Papaya seeds were used to isolate endophytic bacteria, which were then examined for antibacterial activity. The dics diffusion method is used to determine the antibacterial activity's efficacy against Escherichia coli and Staphylococcus aureus. There are nine isolates of endophytic bacteria in all. According to a potency test, the nine isolates exhibit strong antibacterial activity against Escherichia coli and Staphylococcus aureus, respectively. Every isolate has the ability to effectively inhibit Staphylococcus aureus. The only isolates that could suppress Escherichia coli were CPA 5, CPA 6, and CPA 8. Based on their antibacterial activity against Escherichia coli and Staphylococcus aureus, it was determined that the endophytic bacterial isolates from papaya seed have the capacity to generate antibacterials.

References

Afzal, I., Shinwari, Z. K., Sikandar, S., & Shahzad, S. (2019). Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiological Research, 221(December 2018), 36–49. https://doi.org/10.1016/j.micres.2019.02.001

AR, M. M., Lestari, M. D., Setiawati, U. N., Setyaningrum, E., Nukmal, N., Arifiyanto, A., & Aeny, T. . (2022). Uji Daya Hambat Pertumbuhan Mikroba Patogen Oleh Streptomyces sp. strain 118 Sebagai Agen Biokontrol. Bioeskperimen, 8(2), 88–96.

Ariani, N., Monalisa., & Febrianti, D. R. (2019). Uji Aktivitas Antibakteri Ekstrak Biji Pepaya (Carica papaya L.) Terhadap Pertumbuhan Escherichia coli (Test Activity of Antibacterial Pepaya Seeds (Carica papaya L.) on Growth of Escherichia coli). Journal of Current Pharmaceutical Sciences, 2(2), 2598–2095. journal.umbjm.ac.id/index.php/jcps

Azyenela, L., Purnama Sari, H., Supri Wardi, E., & Sartika, D. (2023). Isolation and identification with 16s rRNA gene of endophytic bacteria from papaya (Carica papaya L.) and test of its antibacterial activity. Journal of Pharmaceutical and Sciences, 6(3), 1068–1078.

Buang, A., Isnaeni, D., & Nurhunaida, E. (2019). Uji Efektivitas Antibakteri Ekstrak Kulit Buah Pepaya (Carica Papaya L.)Terhadap Propioni bacterium acnes Ariani. Majalah Farmasi Nasional, 16(01), 13–20.

Faujiah, P., Hijriani, B. I., & Kurniawan, E. (2023). The Inhibition Activity Of Endophytic Bacteria Of Papaya Leaves (Carica papaya L.) Against Staphylococcus aureus. Journal of Natural Sciences, 1(4), 101–106.

Ginting, L., Wijanarka, & Kusdiyantini, E. (2020). Isolasi bakteri endofit tanaman pepaya (Carica papaya L.) dan uji aktivitas enzim amilase. Berkala Bioteknologi, 3(2), 1–7.

Gond, S. K., Bergen, M. S., Torres, M. S., & White, J. F. (2015). Endophytic Bacillus spp. produce antifungal lipopeptides and induce host defence gene expression in maize. Microbiological Research, 172, 79–87. https://doi.org/10.1016/j.micres.2014.11.004

Hardoim, P. R., van Overbeek, L. S., Berg, G., Pirttilä, A. M., Compant, S., Campisano, A., Döring, M., & Sessitsch, A. (2015). The Hidden World within Plants: Ecological and Evolutionary Considerations for Defining Functioning of Microbial Endophytes. Microbiology and Molecular Biology Reviews, 79(3), 293–320. https://doi.org/10.1128/mmbr.00050-14

Jamilatun, M., Aminah, A., & Shufiyani, S. (2020). Uji Daya Hambat Antibakteri Kapang Endofit Dari Tanaman Alang-Alang (Imperata Cylindrica (L.) Beauv.) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Dan Escherichia Coli. Jurnal Medikes (Media Informasi Kesehatan), 7(2), 335–346. https://doi.org/10.36743/medikes.v7i2.224

Liu, H., Carvalhais, L. C., Crawford, M., Singh, E., Dennis, P. G., Pieterse, C. M. J., & Schenk, P. M. (2017). Inner plant values: Diversity, colonization and benefits from endophytic bacteria. Frontiers in Microbiology, 8(DEC), 1–17. https://doi.org/10.3389/fmicb.2017.02552

Mahdi, R. A., Bahrami, Y., & Kakaei, E. (2022). Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica. Scientific Reports, 12(1), 1–13. https://doi.org/10.1038/s41598-022-23073-4

Mai, P. Y., Levasseur, M., Buisson, D., Touboul, D., & Eparvier, V. (2020). Identification of antimicrobial compounds from sandwithia guyanensis-associated endophyte using molecular network approach. Plants, 9(1). https://doi.org/10.3390/plants9010047

Peter, J. K., Kumar, Y., Pandey, P., & Masih, H. (2014). Antibacterial Activity of Seed and Leaf Extract of Carica Papaya var. Pusa dwarf Linn. IOSR Journal of Pharmacy and Biological Sciences, 9(2), 29–37. https://doi.org/10.9790/3008-09272937

Pratiwi, E. ., Suryani, E. ., Prasetya, A. I. ., & Al Batati, N. (2024). KARAKTERISASI DAN POTENSI BAKTERI ENDOFIT AKAR KOPI (Coffea sp) SEBAGAI PENGHASIL INDOLE ACETIC ACID (IAA). Bio-Lectura : Jurnal Pendidikan Biologi, 11(1), 77–92. https://doi.org/10.31849/bl.v11i1.19229

Pratiwi, E. R., Ardyati, T., & Suharjono, S. (2020). Plant Growth Promoting Endophytic Bacteria of Coffea canephora and Coffea arabica L. in UB Forest. The Journal of Experimental Life Sciences, 10(2), 119–126. https://doi.org/10.21776/ub.jels.2020.010.02.07

Riu, F., Ruda, A., Ibba, R., Sestito, S., Lupinu, I., Piras, S., Widmalm, G., & Carta, A. (2022). Antibiotics and Carbohydrate-Containing Drugs Targeting Bacterial Cell Envelopes: An Overview. Pharmaceuticals, 15(8), 1–38. https://doi.org/10.3390/ph15080942

Sarkar, P., Yarlagadda, V., Ghosh, C., & Haldar, J. (2017). A review on cell wall synthesis inhibitors with an emphasis on glycopeptide antibiotics. MedChemComm, 8(3), 516–533. https://doi.org/10.1039/c6md00585c

Semenzato, G., Faddetta, T., Falsini, S., Del Duca, S., Esposito, A., Padula, A., Greco, C., Mucci, N., Zaccaroni, M., Puglia, A. M., Papini, A., & Fani, R. (2022). Endophytic Bacteria Associated with Origanum heracleoticum L. (Lamiaceae) Seeds. Microorganisms, 10(10). https://doi.org/10.3390/microorganisms10102086

Sharma, M., & Mallubhotla, S. (2022). Diversity, Antimicrobial Activity, and Antibiotic Susceptibility Pattern of Endophytic Bacteria Sourced From Cordia dichotoma L. Frontiers in Microbiology, 13(May), 1–17. https://doi.org/10.3389/fmicb.2022.879386

Singh, M., Kumar, A., Singh, R., & Pandey, K. . (2017). Endophytic bacteria: a new source of bioactive compounds. 3 Biotech, 7(315).

Subramani, R., Narayanasamy, M., & Feussner, K. D. (2017). Plant-derived antimicrobials to fight against multi-drug-resistant human pathogens. 3Biotech, 7(172).

Taulé, C., Vaz-Jauri, P., & Battistoni, F. (2021). Insights into the early stages of plant–endophytic bacteria interaction. World Journal of Microbiology and Biotechnology, 37(1). https://doi.org/10.1007/s11274-020-02966-4

Torar, G. M. J., Lolo, W. A., & Citraningtyas, G. (2017). Uji Aktivitas Antibakteri Ekstrak Etanol Biji Pepaya (Carica papaya L.) Terhadap Bakteri Pseudomonas aeruginosa DAN Staphylococcus aureus. PHARMACONJurnal Ilmiah Farmasi, 6(2), 14–22. https://repository.unugiri.ac.id/id/eprint/643/

Truyens, S., Weyens, N., Cuypers, A., & Vangronsveld, J. (2015). Bacterial seed endophytes: Genera, vertical transmission and interaction with plants. Environmental Microbiology Reports, 7(1), 40–50. https://doi.org/10.1111/1758-2229.12181

Vital, P. G., Velasco, R. N., Demigillos, J. M., & Rivera, W. . (2010). Antimicrobial Activity and Phy tochemical Screening of Ficus septic and Stercu lia foetida L. Leaf Extracts. Journal of Medicinal Plants Research, 4(1), 058–063.

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Antibacterial of Endophytic Bacteria from Papaya (Carica papaya) Seeds Againts Staphylococcus aureus and Escherichia coli

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