Isolation and Characterization of Thermophilic Bacteria as Producing Enzyme Amylase from Kepanasan Hot Springs In Tapung Hulu, Kampar, Riau
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Fadia Rezika Herwanda , Tetty Marta LindaDOI:
10.29303/jbt.v25i1.7888Published:
2025-03-14Issue:
Vol. 25 No. 1 (2025): Januari - MaretKeywords:
Amylase enzymes, amylolytic index, kepanasan hot springs, thermophilic bacteria.Articles
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Abstract
Thermophilic bacteria are a group of bacteria that can live and survive at high temperatures ranging from 45°C-80°C. Thermophilic bacteria are the best choice as a producer of thermostable enzymes that can endure high temperatures used in various industrial fields including the textile industry, food industry, detergent industry, and pharmaceutical industry. This study aims to isolate, characterize, and test the activity of thermostable amylase enzyme from thermophilic bacterial isolates in Kepanasan hot spring in Tapung Hulu District, Kampar Regency, Riau. Characterization of thermophilic bacterial isolates is macroscopic, physiological, and biochemical tests: Triple sugar iron agar, Sulphur indole motility, symmons citrate, and catalase. The results of study obtained ten isolates of thermophilic bacteria consisting of six isolates from hot water samples and four isolates from soil sediments isolated at temperatures of 45°C and 50°C using a nutrient agar (NA) medium. The results of Gram staining were obtained one Gram-positive isolate and nine Gram-negative isolates. Positive catalase test results, negative indole and motility tests for ten isolates. The results of the amylase enzyme activity test obtained three bacterial isolates, namely BTA 6, BTT 2 and BTT 4 with the highest amylolytic index of 1.32 produced by BTT 2 isolate at an incubation temperature of 50°C with a starch concentration of 1%.
References
Alawiyah DN, Astuti W, Hairani R. (2021). Isolasi bakteri penghasil lipase dari sumber air panas Long Kali. Jurnal Atomik 6(1): 32-34. ISSN 2549-0052. DOI :
Brock TD, Brock KM, Ward DM. (1986). Basic Microbiology with Applications. Madison: Prentice-Hall.
Devi M. (2019). Isolasi, identifikasi morfologi, dan uji aktivitas enzim hidrolitik dari bakteri termofilik sumber air panas Pawan Rokan Hulu, Riau [skripsi]. Pekanbaru: Universitas Riau.
Gazali FM, Suwastika IN. (2018). Thermostable amylase activity from thermophilic bacteria isolated from Bora Hot Spring, Central Sulawesi. Journal of Physics 2:1-6. DOI: 10.1088/17426596/979/1/012001
Ginting EL. (2020). Penapisan dan karakterisasi bakteri proteolitik termofilik dari sumber air panas Pantai Moinit, Sulawesi Utara. Jurnal Ilmiah Platax 8(1)118-125. DOI : https://doi.org/10.35800/jip.8.1.2020.28628
Hardi J, Jusman, Razak AR, Silva. (2016). Produksi dan uji aktivitas enzim kitinase dari isolat bakteri termofilik b1211 asal air panas Bora. Jurnal Kovalen 2(3):67-72. DOI : https://www.neliti.com/ publications/144220/produksi-dan-uji-aktivitas-enzim-kitinase-dari-isolat-bakteri-termofilik-b1211-a#cite
Huwae LM, Aditiawati P. (2020). Isolasi dan identifikasi bakteri termofilik penghasil enzim selulase dari sumber air panas Sila. Jurnal Ilmu Kelautan dan Perikanan Papua 3(1):32-35. DOI: https://doi.org/10.31957/acr.v3i1.1214
Indriati G, Megahati R. (2021). Thermozyme Amylase from Enterobacter sp Extremophiles in Bioethanol Production. Journal of Science and Science Education 2(1): 1-7. DOI.org/10.29303/jossed.v2i1.698
Linda TM, Devi S, Roza RM, Silaban MDU. (2015). Aktivitas protease alkalin oleh bakteri termofilik alkalitoleran dari sumber air panas Desa Sungai Pinang Kabupaten Kuantan Singingi, Riau. Prosiding Semirata Bidang MIPA BKS-PTN Barat. hlm. 351-358. DOI : https://jurnal.untan.ac.id/index.php/semirata2015/article/view/13757
Lingga R, Afriyansyah B, Septiani R, Miranti I. (2021). Isolasi dan Karakterisasi Bakteri Asal Sumber Air Panas Non-Vulkanik. Bioedusains: Jurnal Pendidikan Biologi dan Sains 4(2): 175-184. DOI : https://doi.org/10.31539/bioedusains.v4i2.2423
Majidah R, Lubis SS, Harahap D. (2023). Potensi enzim selulase dari bakteri termofilik di kawasan Wisata Ie Suum Kabupaten Aceh Besar. Journal of Biological Sciences and Applied Biology 3(1): 25-34. DOI: 10.22373/kenanga.v3i1.3119
Mawati SD, Harpeni E, Fidyandini HP. (2021). Skrining bakteri termofilik potensial amilolitik dari sumber air panas Way Belerang Kalianda Lampung Selatan. Journal of Aquatropica Asia 6(1):1-7. DOI: 10.33019/aquatropica.v6i1.2458
Megahati RRP. (2017). Bakteri termofilik penghasil selulase asal sumber air panas di Indonesia. Jurnal Bioconcetta 3(1):61-68. 22202/bc.2017.v3i1.2728
Nanda PT, Siregar SA, Kurniawan R, Meriyanti H, Yatno. (2017). Isolasi, karakterisasi dan uji potensi bakteri penghasil enzim termostabil Air Panas Kerinci. Chempublish Journal 2(1):26-31. DOI : https://online-journal.unja.ac.id/chp/article/view/3238
Pitri RE, Agustien A, Febria FA. (2015). Isolasi dan karakterisasi bakteri amilotermofilik dari sumber air panas Sungai Medang. Jurnal Biologi Universitas Andalas 4(2):119-122. DOI: https://doi.org/10.25077/jbioua.4.2.%25p.2015
Rana N, Verma N, Vaidya D, Ghabru A. (2017). Application of amylase producing bacteria isolated from hotspring water in food industry. Annals of Phytomedicine 6(2):93-100. DOI:10.21276/ap.2017.6.2.9
Runtuboi DYP, Gunaedi T, Simonapendi M, Pakpahan N. (2018). Isolasi dan identifikasi bakteri termofilik dari sumber air panas di Moso Distrik Muara Tami Kota Jayapura Provinsi Papua. Jurnal Biologi Papua 10(2):68-73. DOI: https://doi.org/10.31957/jbp.474
Septiana L, Susanti E, Haryani Y, Rani Z, Rambe R. (2024). Penapisan dan karakterisasi bakteri selulolitik termofilik. Forte Journal 4(2):472-480. DOI: https://doi.org/10.51771/fj.v4i2.974
Sharma H, Batra N, Singh J. (2022). Purification, characterization and potential detergent industry application of a thermostable α-amylase from Bacillus licheniformis RA31. Indian Journal of Experimental Biology 60:331-342. DOI: https://doi.org/10.56042/ijeb.v60i05.35491.
Suharti N, Putra G. (2022). Isolasi dan identifikasi bakteri termofilik penghasil amilase dari sumber air panas Sidebuk-Debuk Sumatera Utara. Jurnal Ilmiah Pannmed 17(1):1-11. DOI: 10.36911/pannmed.v17i1.1277
Syafriyani D, Agustien A, Periadnadi. (2013). Penapisan bakteri termo-amilolitik dari sumber air panas Sungai Medang, Kerinci, Jambi. Jurnal Biologi Universitas Andalas 2(2):77-82. DOI: https://doi.org/10.25077/jbioua.2.2.%25p.2013.
Zafar A, Aftab MN, Iqbal I, Din ZU, Saleem MA. (2019). Pilot-scale production of a highly thermostablea-amylase enzyme from Thermotoga petrophila cloned into E. coli and its application as a desizer intextile industry. Royal Society of Chemistry 9:984-992. DOI: https://doi.org/10.1039/C8RA06554C
Zuraidah, Wahyuni D, Astuty E. (2020). Karakteristik morfologi dan uji aktivitas bakteri termofilik dari kawasan wisata Ie Seuum. Jurnal Ilmu Alam dan Lingkungan 11(2):40-47. DOI: 10.20956/jal.v11i2.10200
Author Biography
Tetty Marta Linda, Prodi Biologi Jurusan Biologi FMIPA Universitas Riau Pekanbaru
Prodi Biologi FMIPA
Universitas Riau
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