Identification of Bacteria in Fresh Vegetables from The Traditional Market of Mataram City
DOI:
10.29303/jbt.v22i4.4445Published:
2022-12-17Downloads
Abstract
Indonesia is very rich in potential for vegetable cultivation. Vegetables contain a lot of vitamins and minerals. Therefore, people prefer to eat vegetables in a fresh state either as salads or fresh vegetables. However, contamination of pathogenic bacteria is much greater both at the farmer and trader levels. Therefore this study aims to determine the feasibility of consumption and identify bacteria in fresh vegetables in the traditional market of Mataram City. Samples in the form of lettuce, cucumber, cabbage, and long beans were taken from Pagesangan, Kebon Roek, Gunung Sari, and Bertais markets, respectively. The existence of bacteria were detected using traditional method by inoculating samples in agar cultures followed by biochemical testing and Gram staining. Species of bacteria that can be identified from samples are Enterobacter cloacae, Bhanhamella catarrhalis, E-coli, Staphylococus sapropyticus, and Aeromonas hydrophila. Based on the data, long beans from the Bertais market and lettuce from the Pagesangan, Kebon Roek, and Gunung Sari markets are not suitable for consumption because the TPC yield exceeds the consumption quality standard of 105-106 CFU/g.
Keywords:
bacteria pathogen; TPC; vegetable quality standardsReferences
Azhar, S. W. (2020). Efektivitas Ekstrak Kulit Petai (Parkia Spesioca Hassk) Sebagai Disinfektan Alami Pada Sayuran Segar. Diploma thesis, Politeknik Negeri Jember.
Bandangan, A. A. (2016). Hubungan Antara Faktor Risiko Infeksi Saluran Pernafasan Akut Dengan Terjadinya Otitis Media Akut Pada Balita. Thesis, Universitas Muhammadiyah Yogyakarta.
Barakat S. M. M. (2011). Salmonella – A Dangerous Foodborne Pathogen. InTech: Croatia.
BSN. (2009). Batas maksimum cemaran mikroba dalam pangan. SNI 7388: ICS 67.220.20
Huda, M. (2013). Pengaruh Madu Terhadap Pertumbuhan Bakteri Gram Positif (Staphylococcus Aureus) Dan Bakteri Gram Negatif (Escherichia Coli). Jurnal Analis Kesehatan, 2(2).
Hutasoit, D. P. (2020). Pengaruh Sanitasi Makanan dan Kontaminasi Bakteri Escherichia coli Terhadap Penyakit Diare. Jurnal Ilmiah Kesehatan Sandi Husada, 9(2).
Idar, Kusumawardhani, S., & Novianti, M. T. (2018). Deteksi Bakteri Patogen Salmonella typhipada Sayuran Mentah Menggunakan Metodenested Polymerase Chain Reaction. Jurnal Al-Kimia, 6(2), 146-154.
Iqbal, M. (2019). Perbandingan Jumlah Bilangan Kuman Pada Sayur Lalapan Dengan Perlakuan Pencucian Yang Berbeda. Medan: Politeknik Kesehatan Kemenkes RI Medan.
Kementerian Kesehatan Republik Indonesia. (2018). Tabel Komposisi Pangan Indonesia 2017. Jakarta: Kementrian Kesehatan RI.
Kustyawati, M. E. (2020). Mikrobiologi hasil pertanian. Bandar Lampung: Pusaka Media. ISBN: 978-623-7560-96-8.
Metisya, H. (2016). Perbedaan Pencucian Menggunakan Air Mengalir Dan Menggunakan Teknik Blansir Terhadap Pertumbuhan Koloni Bakteri Pada Lalapan Selada (Lactuca Sativa L.) Di Warung Makan Kelurahan Jati Kota Padang. Diploma thesis, Universitas Andalas.
Muslikha, Pujiyanto, S, & Jannah, SN (2016). Isolasi, Karakterisasi Aeromonas Hydrophila dan Deteksi Gen Penyebab Penyakit Motile Aeromonas Septicemia (MAS) dengan 16s Rrna dan Aerolysin Pada Ikan Lele (Clarias Sp.). Jurnal Biologi, 5(4).
Primaharani, O. (2021). Efektivitas Serbuk Ekstrak Kulit Jengkol (Pithecellobium lobatum Benth) Sebagai Disinfektan Alami Pada Sayuran Segar. Diploma thesis, Politeknik Negeri Jember.
Riga, P. N., Buntuan, V & Rares, F (2015). Isolasi dan Identifikasi Bakteri Aerob yang Dapat Menyebabkan Infeksi Nosokomial di Ruangan Instalasi Gizi BLU RSUP prof. Dr. R. D. Kandou Manado. Jurnal e-Biomedik (eBm), 3(1).
Setiawan, Y. S. (2020). Deteksi Cepat Listeria monocytogenes pada Sayuran di Kota Malang Menggunakan Metode Real Time Polymerase Chain Reaction (qPCR). Thesis, Universitas Brawijaya.
Siahaan, R. O. I. (2010). Isolasi salmonella sp. Pada Sayuran Segar Di Wilayah Bogor dan Evaluasi Pengaruh Perlakuan Pencucian dengan Sanitaiser Komersial (Skripsi). Bogor: Institut Pertanian Bogor.
Silviani, Y., & Saktiningsih, H. (2020).Pemberdayaan Masyarakat dalam Pencegahan Demam Typhoid dengan Pemanfaatan Antiseptik Jus Daun Sirih Hijau Sebagai Pencuci Buah dan Sayur. JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat), 4(2).
Soesetyaningsih, E., & Azizah. (2020). Akurasi Perhitungan Bakteri pada Daging Sapi Menggunakan Metode Hitung Cawan. BERKALA SAINSTEK, VIII (3): 75-79.
Sopandi, W. T. (2014). Mikrobiologi Pangan. Yogyakarta: Penerbit Andi.
Suryani, D. (2012). Hubungan Perilaku Mencuci dengan Kontaminasi Telur Nematode Usus pada Sayuran Kubis (Brassica Oleracea) Pedagang Pecel Lele di Kelurahan Warungboto Kota Yogyakarta. Jurnal KES MAS UAD, 6(2), 162-232.
License
Copyright (c) 2022 Syamsul Bahri, Rika Ratnasari, Ulfah Nurkhaeroni, Mujitahid Mujitahid
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.
