The Potential of Gelam (Melaleuca leucadendra) Bark Extract as an Antibacterial Agent Against Pseudomonas aeruginosa and Enterococcus faecalis

Fathul Yusro; Yeni Mariani

doi:10.29303/jbt.v25i3.9012

The Potential of Gelam (Melaleuca leucadendra) Bark Extract as an Antibacterial Agent Against Pseudomonas aeruginosa and Enterococcus faecalis

Authors

Fathul Yusro , Yeni Mariani

DOI:

10.29303/jbt.v25i3.9012

Published:

2025-07-09

Issue:

Vol. 25 No. 3 (2025): Juli-September

Keywords:

Antibacterial, Enterococcus faecalis, Gelam bark, Melaleuca leucadendra, Pseudomonas aeruginosa.

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Yusro, F., & Mariani, Y. (2025). The Potential of Gelam (Melaleuca leucadendra) Bark Extract as an Antibacterial Agent Against Pseudomonas aeruginosa and Enterococcus faecalis. Jurnal Biologi Tropis, 25(3), 2584–2589. https://doi.org/10.29303/jbt.v25i3.9012

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Abstract

This study aims to evaluate the antibacterial activity of Melaleuca leucadendra (Gelam) bark extract against Pseudomonas aeruginosa and Enterococcus faecalis. Bark samples were collected from Karimunting Village, Bengkayang Regency, and extracted using maceration with 96% ethanol. Antibacterial testing was conducted using the disc diffusion method on MHA (Mueller Hinton Agar), with bacterial suspensions adjusted to McFarland standard 1. The results showed that the extract exhibited concentration-dependent antibacterial activity. The highest inhibition zones were observed at a concentration of 200 mg/mL, measuring 4.5 mm for P. aeruginosa and 3.5 mm for E. faecalis. Although the inhibitory effect was relatively low compared to the positive control (amoksilin), these findings indicate the potential of Gelam bark extract as a natural antibacterial agent. Further studies are recommended to isolate active compounds, assess toxicity, and develop formulations to enhance its antibacterial efficacy.

References

Abers, M., Schroeder, S., Goelz, L., Sulser, A., St. Rose, T., Puchalski, K., & Langland, J. (2021). Antimicrobial activity of the volatile substances from essential oils. BMC Complementary Medicine and Therapies, 21(1), 1–4. https://doi.org/10.1186/s12906-021-03285-3

Ahmed, S. N., Al Touby, S. S., & Hossain, M. A. (2023). Isolation and evaluation of significant antibacterial fraction of methanol and its derived fractions from the leaves extract of Zygophyllum simplex. Advances in Biomarker Sciences and Technology, 5, 1–7. https://doi.org/10.1016/j.abst.2023.01.001

Anggita, D., Nuraisyah, S., & Wiriansya, E. P. (2022). Mekanisme Kerja Antibiotik Open Access ABSTRAK. UMI Medical Journal, 7, 46–58.

Filbert, K., Wijaya, S., Budi, A., & Tobing, A. N. L. (2023). Uji Aktivitas Antibakteri Ekstrak Kulit Jeruk Bali (Citrus maxima pericarpium) Terhadap Pseudomonas aeruginosa dan Enterococcus faecalis. Jambura Journal of Health Science and Research, 5(1), 51–58. https://ejurnal.ung.ac.id/index.php/jjhsr/index

Gauba, A., & Rahman, K. M. (2023). Evaluation of Antibiotic Resistance Mechanisms in Gram-Negative Bacteria. Antibiotics, 12(11), 1–30. https://doi.org/10.3390/antibiotics12111590

Hidayatullah, S. H., & Mourisa, C. (2023). Uji Efektivitas Akar Karamunting (Rhodomyrtus Tomentosa (Aiton) Hassk) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus. Jurnal Ilmiah Kohesi, 1, 34–40.

Kaczmarek, B. (2020). Tannic acid with antiviral and antibacterial activity as a promising component of biomaterials-A minireview. Materials, 13(14), 1–13. https://doi.org/10.3390/ma13143224

Kesuma, S., Saputri, J., & Alekandra, P. (2023). Profil Bakteri Penginfeksi Pus Pada Luka Di Laboratorium Mikrobiologi Rsud Abdoel Wahab Sjahranie Periode Bulan Januari-Juni Tahun 2023. Jurnal Kesehatan Tambusai, 4(4), 6013–6023.

Mariani, Y., Yusro, F., & Wardenaar, E. (2020). Aktivitas Ekstrak Metanol Daun Ulin (Eusideroxylon zwageri Tisjm & Binn) Terhadap Empat Jenis Bakteri Patogen. Jurnal Biologi Tropis, 20(1), 94–101. https://doi.org/10.29303/jbt.v20i1.1642

Masyudi, Noviyanti, A., Ridhwan, M., Nurman, S., Jailani, Armi, Rafsanjani, T. M., Usman, S., Hanafiah, M., & Marlina. (2023). Antibacterial activity of Blumea balsamifera leaf extracts from Aceh, Indonesia. Biodiversitas, 24(8), 4584–4589. https://doi.org/10.13057/biodiv/d240862

Nababan, F., Panjaitan, I. M. S., & Ricky, D. R. (2025). Antibacterial Effectiveness Test of Roselle Flower (Hibiscus sabdariffa L.) Ethanol Extract Against Escherichia coli, Staphylococcus aureus, and Propionibacterium acnes Bacteria. Jurnal Biologi Tropis, 25(1), 1074–1083. https://doi.org/10.29303/jbt.v25i1.8765

Noor, A. A. M. (2023). Melaleuca cajuputi Powell Essential Oil: A Review of Botanical, Phytochemical and Pharmacological Properties. Borneo Journal of Resource Science and Technology, 13(2), 1–12. https://doi.org/10.33736/bjrst.5314.2023

Setiawan, E., Royland Marpaung, F., Sukandar, E., Lily Lukas, D., Wijono, H., Warindra, T., Kurniawan, R., Wibowo, T., Hendradi, W., Osbert Costa, M., Abdul-Aziz, M.-H., & Roberts, J. (2019). Kajian Narrative terhadap Profil Farmakokinetik Antibiotik pada Pasien Kritis: Implikasi terhadap Ketercapaian Target Farmakokinetik-Farmakodinamik. Pharmaceutical Sciences and Research, 6(1), 1–12.

Sinarsih, N. K., Susanah Rita, W., & Puspawati, N. M. (2021). Aktivitas Antibakteri Fraksi Ekstrak Etanol Daun Trembesi (Samanea saman (Jacq.) Merr) terhadap Staphylococcus aureus. International Journal of Applied Chemistry Research |, 3(1), 2549–3671. https://doi.org/10.23887/ijacr-undiksha

Sudiansyah, M. I., Yusro, F., & Mariani, Y. (2023). Aktivitas Antibakteri Ekstrak Kulit Batang Gelam (Melaleuca leucadendra Linn.) terhadap Salmonella enterica serovar Typhimurium. Jurnal Serambi Engineering, VIII(3), 6161–6167.

Sulaiha, S., Mustikaningtyas, D., Widiatningrum, T., & Dewi, P. (2022). Senyawa Bioaktif Trichoderma erinaceum dan Trichoderma koningiopsis Serta Potensinya Sebagai Antibakteri. 11(2), 120–131.

Tan, C. A. Z., Lam, L. N., Biukovic, G., Soh, E. Y. C., Toh, X. W., Lemos, J. A., & Kline, K. A. (2022). Enterococcus faecalis Antagonizes Pseudomonas aeruginosa Growth in Mixed-Species Interactions. Journal of Bacteriology, 204(7), 1–16. https://doi.org/10.1128/jb.00615-21

Tang, K. W. K., Millar, B. C., & Moore, J. E. (2023). Antimicrobial Resistance (AMR). British Journal of Biomedical Science, 80, 1–11. https://doi.org/10.3389/bjbs.2023.11387

Unok, W., & Sabir Mangawing, M. (2024). Resistensi Antibiotik Terhadap Infeksi Saluran Kemih (ISK): Literature Review Antibiotic Resistance in Urinary Tract Infections : Literature Review Artikel Review. Jurnal Kolaboratif Sains, 7(5), 1822–1828. https://doi.org/10.56338/jks.v7i5.5347

Wahyudi, D., Silviani, Y., Nirwana, A. P., & Saroh, D. (2024). Deteksi Gen Resisten Kloramfenikol (cat) pada Isolat Klinik Pseudomonas aeruginosa dan Escherichia coli dengan Metode Polymerase Chain Reaction. Sciscitatio, 5(1), 10–19.

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The Potential of Gelam (Melaleuca leucadendra) Bark Extract as an Antibacterial Agent Against Pseudomonas aeruginosa and Enterococcus faecalis

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