Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS

Tri Haidar Munif; Marjori Bethania Rahmawan; Nurlita Abdulgani; Endry Nugroho Prasetyo

doi:10.29303/jbt.v25i3.9474

Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS

Authors

Tri Haidar Munif , Marjori Bethania Rahmawan , Nurlita Abdulgani , Endry Nugroho Prasetyo

DOI:

10.29303/jbt.v25i3.9474

Published:

2025-07-02

Issue:

Vol. 25 No. 3 (2025): Juli-September

Keywords:

Cellobiosa dehydrogenase, enzyme, Trametes versicolor.

Articles

Downloads

pdf

How to Cite

Munif, T. H., Rahmawan, M. B., Abdulgani, N., & Prasetyo, E. N. (2025). Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS. Jurnal Biologi Tropis, 25(3), 2404–2409. https://doi.org/10.29303/jbt.v25i3.9474

Download Citation

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Cellobiose dehydrogenase (CDH, EC 1.1.99.18) is an enzyme classified under the oxidoreductase group. CDH has been widely utilized in various fields, including healthcare such as in wound dressing and in the energy sector for bioenergy production. To support these industrial applications, it is necessary to investigate the characteristics of CDH to enhance process efficiency. Therefore, this study aims to characterize CDH produced by the fungus Trametes versicolor strain TRAMS. The research involved constructing a CDH production curve followed by enzyme activity assays, protein content determination, isoelectric point (pI) analysis, and kinetic studies using Michaelis-Menten and Lineweaver-Burk plots. The results showed that the enzyme exhibited an activity of 3.441 U/mL on the 8th day of production. The enzyme's specific activity reached 61.57 U/mg at the 80% ammonium sulfate fraction, with an isoelectric point at pH 4. Enzyme kinetics based on the Michaelis-Menten plot revealed a V_max of 6.7 µM/min and a K_m of 3.35 µM, while the Lineweaver-Burk analysis indicated a V_max of 9.625 µM/min and a K_m of 0.23 µM.

References

Canam, T., Town, J. R., Tsang, A., McAllister, T. A., & Dumonceaux, T. J. (2011). Biological pretreatment with a cellobiose dehydrogenase deficient strain of Trametes versicolor enhances the biofuel potential of canola straw. Biosource Technology, 102(21). DOI: https://doi.org/10.1016/j.biortech.2011.08.045

Chen, Y., Chen, J., Chang, C., Chen, J., Cao, F., Zhao, J., Zheng, Y., & Zhu, J. (2019). Physicochemical and functional properties of proteins extracted from three microalgal species. Food Hydrocolloids, 96. DOI: https://doi.org/10.1016/j.foodhyd.2019.05.025

Duong-Ly, K. C. & Gabelli, S. B. (2014). Salting out of Proteins Using Ammonium Sulfate Precipitation. Methods in enzymology, 541. 85-94. DOI: http://dx.doi.org/10.1016/B978-0-12-420119-4.00007-0

Felice, A. K. G., Schuster, C., Kadek, A., Filandr, F., Laurent, C. V. F. P., Scheiblbrandner, S., Schwaiger, L., Schachinger, F, Kracher, D., Sygmund, C., Man, P., Halada, P., Oostenbrink, C., & Ludwig, R. (2021). Chimeric Cellobiose Dehydrogenases Reveal the Function of Cytochrome Domain Mobility for the Electron Transfer to Lytic Polysaccharide Monooxygenase. ACS Publications, 11(2). 517-532. DOI: https://doi.org/10.1021%2Facscatal.0c05294

Geiss, A. F., Reichart, T. M. B., Pejker, B., Plattner, E., Herzog, P. L., Schulz, C., Ludwig, R., Felice, A. K. G., & Haltrich, D. (2021). Engineering the Turnover Stability of Cellobiose Dehydrogenases toward Long-term ioelectronic Applications. ACS Sustainable Chemistry & Engineering, 9(20). 7086-7100. DOI: https://doi.org/10.1021/acssuschemeng.1c01165

Giorgianni, A., Zenone, A., Sützl, L., Csarman, F., & Ludwig, R. (2024). Exploring class III cellobiose dehydrogenase: sequence analysis and optimized recombinant expression. Microbial Cell Factories, 23(146). DOI: https://doi.org/10.1186/s12934-024-02420-2

Greene, B. L. (2021). Progress and Opportunities in Photochemical Enzymology of Oxidoreductases. ACS Cataliysis, 11(23). 14635-14650. DOI: https://doi.org/10.1021/acscatal.1c04525

Jiang, Y., Tian, Q., Chen, C., Deng, Y., Hu, X., & Yi, Y. (2024). Impact of salting-in/out assisted extraction on rheological biological, and digestive, and proteomic properties of Tenbrio molitor larvae protein isolates. International Journal of Biological Macromolecules, 282(3). DOI: https://doi.org/10.1016/j.ijbiomac.2024.137044

Johnson, K. A., & Goody, R. S. (2011). The Original Michaelis Constant: Translation of the 1913 Michaelis-Menten Paper. Biochemistry, 50(39). 8264-8269. DOI: https://doi.org/10.1021/bi201284u

Karapetyan, K. N., Fedorova, T. V., Vasil'chenko, L. G., Ludwig, R., Haltrich, D., & Rabinovich, M. L. (2006). Properties of neutral cellobiose dehydrogenase from ascomycete Chaetomium sp. INBI 2-26(-) and comparison with basidiomyceteous cellobiose dehydrogenases. Journal of Biotechnology, 121(1). 34-48. DOI: https://doi.org/10.1016/j.jbiotec.2005.06.024

Mahbubillah, M. A., Nurhayati, A. P. D., & Prasetyo, E. N. (2019). The effect of various substrate on production of cellobiose dehydrogenase enzyme by Trametes versicolor. IOP Conf. Series: Materials Science and Engineering, 546. DOI: https://doi.org/10.1088/1757-899X/546/6/062014

Nyanhongo, G. S., Sygmund, C., Ludwig, R., Prasetyo, E. N., & Guebitz, G. M. (2013). An Antioxidant Regenerating system for continous quenching of free radicals in chronic wounds. European Journal of Pharmaceutics and Biopharmaceutics, 83(3). DOI: https://doi.org/10.1016/j.ejpb.2012.10.013

Purwantisari, S., Nurbayani, F. A., Safina, M. F. I., & Choiriyah, M. (2025). Antagonistic activity of Trichoderma harzianum against Aspergillus parasiticus and Mucor circinelloides in corn plant (Zea mays L.). Planta Tropika, 13(1). https://doi.org/10.18196/pt.v13i1.21507

Putri, S. R., Djamaan, A., & Agustien, A. (2025). Growth Curve and Antibacterial ActivityTest of Endophytic Bacteria Isolates 1 (IBE1) from Labu Koteka (Lagenaria siceraria) Against Escherechia coli, Pseudomonas aeruginosa and MRSA. Jurnal Biologi Tropis, 25(2). 1262-1268. http://doi.org/10.29303/jbt.v25i2.8506

Roy, B. P., Dumonceaux, T., Koukoulas, A. A., & Archibald, F. S. (1996). Purification and Characterization of Cellobiose Dehydrogenases from the Whtie Fungus Trametes versicolor. Applied and Environmental Mirobiology, 62(12). 4417-4427. DOI: https://doi.org/10.1128/aem.62.12.4417-4427.1996

Scheiblbrandner, S., & Ludwig, R. (2020). Cellobiose dehydrogenase: Bioelectrochemical insights and applications. Bioelectrochemistry, 131. DOI: https://doi.org/10.1016/j.bioelechem.2019.107345

Yadav, V., & Nighojkar, S. (2023). Cellobiose Dehydrogenase from Schizophyllum commune Bcc26414: Purification and Characterization. Biosciences Biotechnology Research Asia, 20(3). DOI: http://dx.doi.org/10.13005/bbra/3141

Zhong, Y., Guo, Z., Li, M., Jia, X., & Zeng, B. (2024). Expression of cellobiose dehydrogenase gene in Aspergillus niger C112 and its effect on lignocellulose degrading enzymes. Frontiers in Microbiology,15. DOI: https://doi.org/10.3389/fmicb.2024.1330079

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.

The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.

Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.

Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Downloads

Metrics

Abstract

References

License

Most read articles by the same author(s)

Similar Articles

Menu

Manuscript Template

visitor

Keywords

Mitra Kerja sama

Open Access Journals

Contact Us

Information

Share & Follow