Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS

Tri Haidar Munif; Marjori Bethania Rahmawan; Nurlita Abdulgani; Endry Nugroho Prasetyo

doi:10.29303/jbt.v25i3.9474

Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS

Authors

Tri Haidar Munif , Marjori Bethania Rahmawan , Nurlita Abdulgani , Endry Nugroho Prasetyo

DOI:

10.29303/jbt.v25i3.9474

Published:

2025-07-02

Issue:

Vol. 25 No. 3 (2025): Juli-September

Keywords:

Cellobiosa dehydrogenase, enzyme, Trametes versicolor.

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Munif, T. H., Rahmawan, M. B., Abdulgani, N., & Prasetyo, E. N. (2025). Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS. Jurnal Biologi Tropis, 25(3), 2404–2409. https://doi.org/10.29303/jbt.v25i3.9474

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Abstract

Cellobiose dehydrogenase (CDH, EC 1.1.99.18) is an enzyme classified under the oxidoreductase group. CDH has been widely utilized in various fields, including healthcare such as in wound dressing and in the energy sector for bioenergy production. To support these industrial applications, it is necessary to investigate the characteristics of CDH to enhance process efficiency. Therefore, this study aims to characterize CDH produced by the fungus Trametes versicolor strain TRAMS. The research involved constructing a CDH production curve followed by enzyme activity assays, protein content determination, isoelectric point (pI) analysis, and kinetic studies using Michaelis-Menten and Lineweaver-Burk plots. The results showed that the enzyme exhibited an activity of 3.441 U/mL on the 8th day of production. The enzyme's specific activity reached 61.57 U/mg at the 80% ammonium sulfate fraction, with an isoelectric point at pH 4. Enzyme kinetics based on the Michaelis-Menten plot revealed a V_max of 6.7 µM/min and a K_m of 3.35 µM, while the Lineweaver-Burk analysis indicated a V_max of 9.625 µM/min and a K_m of 0.23 µM.

References

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Characterization of Cellobiose Dehydrogenase (CDH) Enzyme in Trametes versicolor strain TRAMS

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