Effectivenessof the Anammox Process for Nitrogen Waste Treatment in Tropical Environments: A Comprehensive Review
Authors
Frentina Murti Sujadi , Jefri Anjaini , Baruna Kusuma , Asro Nurhabib , Lilik Setiyaningsih , Setyo Budi KurniawanDOI:
10.29303/jbt.v25i3.9991Published:
2025-08-21Issue:
Vol. 25 No. 3 (2025): Juli-SeptemberKeywords:
anaerobic; anammox; microbial; wastewater.Articles
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Abstract
The anaerobic ammonium oxidation (Anammox) process is a biological mechanism that converts ammonium and nitrite into nitrogen gas without the need for oxygen, making it a potentially efficient solution for nitrogen waste treatment. This study aims to analyze the effectiveness of Anammox in reducing nitrogen concentrations from various types of waste and compare it with conventional methods. This study was conducted using a systematic literature review approach on the effectiveness of the Anammox process in nitrogen removal from wastewater. The analysis was carried out by extracting information related to reactor types, operational parameters (pH, temperature, C/N ratio, and substrate concentration), nitrogen removal efficiency, and inhibitory factors. The results show that this process can consistently reduce ammonium and nitrite, with performance highly influenced by operational conditions. Factors such as microbial stability, temperature, and nitrite availability are the main determinants of success. This study confirms that Anammox offers a more energy-efficient approach, producing less sludge and being more environmentally friendly compared to nitrification–denitrification. Thus, Anammox technology contributes to the development of sustainable wastewater treatment systems while expanding scientific understanding of the role of Anammox bacteria in the nitrogen cycle.
References
Du, R., Cao, S., Jin, R., Li, X., Fan, J., & Peng, Y. (2022). Beyond an Applicable Rate in Low-Strength Wastewater Treatment by Anammox: Motivated Labor at an Extremely Short Hydraulic Retention Time.. Environmental science & technology. DOI: https://doi.org/10.1021/acs.est.1c05123
Guo, Y., Chen, Y., Webeck, E., & Li, Y. (2019). Towards more efficient nitrogen removal and phosphorus recovery from digestion effluent: Latest developments in the anammox-based process from the application perspective. Bioresource technology. DOI: https://doi.org/10.1016/j.biortech.2019.122560
Jin, R. C., Yang, G. F., Yu, J. J., & Zheng, P. (2012). The inhibition of the Anammox process: A review. Chemical Engineering Journal, 197: 67-79. DOI: https://doi.org/10.1016/j.cej.2012.05.014
Kartal, B., van Niftrik, L., Keltjens, J. T., Op den Camp, H. J. M., & Jetten, M. S. M. (2012). Anammox-Growth Physiology, Cell Biology, and Metabolism. In Advances in Microbial Physiology, 1(60). DOI: https://doi.org/10.1016/B978-0-12-398264-3.00003-6
Li, J., Peng, Y., Zhang, L., Li, X., Zhang, Q., Yang, S., Gao, Y., & Li, S. (2020). Improving Efficiency and Stability of Anammox through Sequentially Coupling Nitritation and Denitritation in a Single-Stage Bioreactor. Environmental Science and Technology, 54(17): 10859-10867. DOI: https://doi.org/10.1021/acs.est.0c01314
Lu, J., Hong, Y., Wei, Y., Gu, J. D., Wu, J., Wang, Y., Ye, F., & Lin, J. G. (2021). Nitrification mainly driven by ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in an anammox-inoculated wastewater treatment system. AMB Express, 11(1). DOI: https://doi.org/10.1186/s13568-021-01321-6
Lulrahman Faldi, Silvia Shinta, & Zulkarnaini. (2022). Penyisihan Nitrogen dengan Proses Anammox Menggunakan Lumpur Muara Penjalinan Kota Padang sebagai Inokulum Nitrogen Removal by Anammox Process Using Sludge from Muara Penjalinan of Padang City as Inoculum. Jurnal Teknologi Lingkungan, 23(2): 143-150.
Lotti, T., Kleerebezem, R., and van Loosdrecht, M. C. M. (2015). Effect of temperature change on anammox activity. Biotechnology and Bioengineering. 112(1): 98-103. DOI: https://doi.org/10.1002/bit.25333
Marlis, I. S., Komala, P. S., dan Zulkarnaini, Z. (2023). Penyisihan nitrogen melalui proses anammox dengan inokulum lumpur instalasi pengolahan air limbah pabrik pupuk. Jurnal Ilmu Lingkungan. 21(3): 717-724. DOI: https://doi.org/10.14710/jil.21.3.717-724
Nabilah Ismail, I., Zulkarnaini, Z., Umar, Z., & Ismail, S. Bin. (2024). Development of an anaerobic ammonium oxidation (ANAMMOX) process reactor for aquaculture wastewater. treatment. Water Science & Technology, 90(6): 1886-1896. DOI: https://doi.org/10.2166/wst.2024.306
Putra, R. P., Komala, P. S., Zulkarnaini, Z., and Komala, P. S. (2020). Start-Up Anammox Process Using Sludge from Koto Baru Lake as Inoculum. Teknologi Lingkungan. 21(2): 138-146.
Putri, W., & Nur, A. (2023). Review Pengolahan Air Limbah Menggunakan Upflow Anaerobic Sludge Blanket (UASB) di Negara Berkembang. Cived, 10(2): 753-765.
Sheng, L., Sheng, L., Lei, Z., Dzakpasu, M., Li, Y., Li, Q., & Chen, R. (2020). Application of the anammox-based process for nitrogen removal from anaerobic digestion effluent: A review of treatment performance, biochemical reactions, and impact factors. Journal of water process engineering, 38. DOI: https://doi.org/10.1016/j.jwpe.2020.101595
Wahyu Wijaya, I. M., & Dharma Putra, P. E. (2021). Anaerobic Ammonium Oxidation (Anammox) Pada Penyisihan Nitrogen Air Limbah Domestik. Jurnal Ecocentrism, 1(2): 113-122. DOI: https://doi.org/10.36733/jeco.v1i2.2425
Witkabel, P., and Abendroth, C. 2024. A systematic literature review of microbial anammox consortia in UASB/ EGSB-reactors. Chemosphere, 367. DOI: https://doi.org/10.1016/j.chemosphere.2024.143630
Xie, H., Ji, D., and Zang, L. 2017. Effects of Inhibition Conditions on Anammox process. IOP. 100: 1-8. DOI: 10.1088/1755-1315/100/1/012149
Zhang, M., Gu, J., Wang, S., & Liu, Y. (2022). A mainstream anammox fixed-film membrane bioreactor with novel sandwich-structured carriers for fast start-up, effective sludge retention and membrane fouling mitigation. Bioresource Technology, 347. DOI: https://doi.org/10.1016/j.biortech.2021.126370
Zhao, Q., Peng, Y., Li, J., Gao, R., Jia, T., Deng, L., & Du, R. (2021). Sustainable upgrading of biological municipal wastewater treatment based on anammox: From microbial understanding to engineering application. The Science of the total environment. DOI: https://doi.org/10.1016/j.scitotenv.2021.152468
Zulkarnaini, Afrianita, R., dan Putra, I. H. (2020). Aplikasi Proses Anammox Dalam Penyisihan Nitrogen Menggunakan Reaktor Up-Flow Anaerobic Sludge Blanket. Jurnal Teknologi Lingkungan. 21(1): 31-39.
Zulkarnaini, Z., Gumelar, G., & Zainuddin, E. (2022). Anaerobic Ammonium Oxidation Performance in Shrimp Pond Wastewater Treatment. Andalasian International Journal of Applied Science, Engineering and Technology, 2(2): 51-56. DOI: https://doi.org/10.25077/aijaset.v2i1.41
Zulkarnaini, Z., Nur, A., & Ermaliza, W. (2019). Nitrogen Removal in the Anammox Biofilm Reactor Using Palm Fiber As Carrier in Tropical Temperature Operation. Jurnal Riset Teknologi Pencegahan Pencemaran Industri, 10(2): 7-15. DOI: https://doi.org/10.21771/jrtppi.2019.v10.no2.p7-15
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