Vol. 23 No. 1 (2023): Special Issue
Open Access
Peer Reviewed

Potensi Aktivitas Antibakteri Minuman Fungsional Kombucha Berbahan Dasar Bunga Kecombrang (Etlingera elatior) Berdasarkan Lamanya Waktu Fermentasi

Authors

Putu Rima Sintyadewi , Pande P Elza Fitriani , Ida Ayu Putu Ary Widnyani , Putu Indrayoni

DOI:

10.29303/jbt.v23i1.6126

Published:

2023-12-06

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Abstract

According to same reports, kombucha is a functional beverage that has the ability to boost immune system function by increasing T cells and containing antimicrobial substances that guard against microbial illness. The antibacterial properties of kombucha derived from kecombrang flowers have not been examined in a any of the numerous studies conducted on the beverage’s antimicrobial activity. The purpose of this study is to investigate how the lengt of fermentation effects the kecombrang flower kombucha antibacterial effectiveness. Fermentation times for the treatments were as follows (P1) 0 days, (P2) 3 days, (P3) 6 days, and (P4) 9 days. The commensal pathogenic microorganisms Escherichia coli, Salmonella typhimurium and Stapylococcus aureus were employed in this study. According to the research findings, the antibacterial activity of kecombrang flower kombucha was significantly impacted (P<0,01) by the duration of fermentation. The maximun antibacterial activity was found in kecombrang flower kombucha that was fermented for nine days (P4). It had an inhibitory power of 12,0 mm against Escherichia coli, 11,5 mm against Salmonella typhimurium, and 14,5 mm against Staphylococcus aureus. Kecombrang flower kombucha is known for its broad spectrum of antibacterial activity, which can suppress the growth of both gram-positif and gram-negatif pathogenic bateria. Based on these results, kecombrang flower kombucha has the potential to be an antibacterial agent and can functions as an alternative approach to functional drinks to control commensal pathogenic bacteria.

Keywords:

Antibacterial, functional drink, kecombrang flower, probiotic.

References

Alizadeh, A.M., Hashempour-Baltork, F., Alizadeh-Sani, M., Maleki, M., Azizi-Lalabad, M. & Khosravi-Darani, K. (2020). Inhibition of Clostridium botulinum and its toxins by probiotic bacteria and their metabolites: an update review. Quality Assurance and Safety of Crops & Foods, 12: 59–68. Doi: https://doi.org/10.15586/qas.v12iSP1.823

Battikh, K., Chaeib, A. Bakhrouf & E. Ammar. (2013). Antibacterial and Antifungal Activities of Black and Green Kombucha Teas. Journal of Food Biochemistry, 37(2): 231-236. Doi: https://doi.org/10.1111/j.1745-4514.2011.00629.x

Bhattacharya, D., Bhattacharya, S., Patra, M.M., Chakravorty, S., Sarkar, S., Chakraborty, W., et al., (2016). Antibacterial activity of polyphenolic fraction of kombucha against enteric bacte- rial pathogens. Current Microbiology 73: 885–896. Doi: https://doi. org/10.1007/s00284-016-1136-3

Bellassoued, K., Ferdaws, G., Fatma, M.A. Josh, V.P., Abdelfattah, E., & Emna, A. (2015). Protective Effect of Kombucha on Rats Fed A Hypercholesterolemic Diet is Mediated by its Antioxidant Activity. Journal Pharmaceutical Biology, 53(11): 199-1709. Doi: https://doi.org/10.3109/13880209.2014.1001408

Bermudez-Brito, M., Plaza-Diaz, J., Munoz-Quezada, S., Gomez-Llorente, C., & Gil, A. (2012). Probiotic mechanisms of action. Ann. Nutr. Metab, 61: 160–174. Doi: https://doi.org/10.1159/000342079

Bhattacharya, S., Gachhui, R., & Sill, P.C. (2013). Effect of Kombucha, a fermented black tea in attenuating oxidative stress mediated tissue demage in alloxan induced diabetic rats. Food and Chemical Toxicology, 60: 328-340. Doi: https://doi.org/10.1016/j.fct.2013.07.051

Bhattacharya, D., Bhattacharya, S., Patra, M. M., Chakravorty, S., Sarkar, S., Chakraborty, W., Koley H., … & Gachhui, R. (2016). Antibacterial activity of polyphenolic fraction of kombucha against enteric bacterial pathogens. Current Microbiology, 73:885–896. Doi: https://doi.org/10.1007/s00284-016-1136-3

Enan, G., Abdel-Shafi, S., Abdel-Haliem, M.F & Negm, S. (2013). Characterization of probiotic lactic acid bacteria to be used as starter and protective cultures for dairy fermentations. Int. J. Probiotics Prebiotics, 8:157–163.

Gao, Z.H., Daliri, E.B.M., Wang, J., Liu, D.H., & Chen, S.G.(2019). Inhibitory effect of lactic acid bacteria on foodborne pathogens: a review. Journal of Food Protection, 82: 441– 453. Doi: https://doi.org/10.4315/0362-028X.JFP-18-303

Harmita & M. Radji (2008). Kepekaan Antibiotik dalam Buku Ajar Analisis Hayati Ed. 3, EGC, Jakarta, pp. 1- 5, 2008

Ilicic, M., Kanuric, K., Milanovic, S., Loncar, E. S., Djuric, M. S., & Malbasa, R. V. (2012). Lactose fermentation by Kombucha-a process to obtain new milk-based beverages. Romanian Biotechnological Letters, 17(1), 7013-7021.

Ismaiel, A.A., Ali, A.E.-S & Enan, G. (2014). Incidence of Listeria in Egyptian meat and dairy samples. Food Sci. Biotechnol, 23: 179–185. Doi: https://doi.org/10.1007/s10068-014-0024-5

Kadyan, S., Rashmi, H.M., Pradhan, D., Kumari, A., Chaudhari, A & Deshwal, G.K. (2021). Effect of lactic acid bacteria and yeast fermentation on antimicrobial, antioxidative and metabolomic profile of naturally carbonated probiotic whey drink. LWT Food Science and Technology, 142: 111059. Doi: https://doi.org/10.1016/j.lwt.2021.111059

Kaewkod, T., Bovonsombut, S. & Tragoolpua, Y. (2019). Efficacy of kombucha obtained from green, oolong, and black teas on inhibition of pathogenic bacteria, antioxidation, and toxicity on colorectal cancer cell line. Microorganisms, 7: 700. Doi: https://doi.org/10.3390/microorganisms7120700

Kallel L, Desseaux V, & Hamdi M. (2012). Insights into the fermentation biochemistry of Kombucha teas and potential impacts of Kombucha drinking on starch digestion. Food Res Int 49:226–32, Doi: http://dx.doi.org/10.1016/j.foodres.2012.08.018

Kumar V & V.K. Joshi. (2016). Kombucha: Technology Microbiology, Production, Composition and Therapeutic Value. Intl. J. Food. Ferment. Technol, 6(1), 13-24. Doi: http://dx.doi.org/10.5958/2277-9396.2016.00022.2

Kohanski, M.A., D.J. Dwyer & J.J. Collins. (2010). How antibiotics kill bacteria: from targets to networks. Nat Rev Microbiol, 8 (6): 423–435. Doi: https://doi.org/10.1038%2Fnrmicro2333

Nurhayati,L.S., Nadhira, Y & Akhmad, H. 2020. Perbandingan Pengujian Aktivitas Antibakteri Stater Yougurt Dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2):41-46. Doi: https://doi.org/10.24198/jthp.v1i2.27537

Osman, A., El-Didamony, G., Sitohy, M., Khalifa, M & Enan, G. (2016). Soybean glycinin basic subunit inhibits methicillin resistant vancomycin intermediate Staphylococcus aureus (MRSA-VISA) in vitro. Int. J. Appl. Res. Nat. Prod. 9: 17–26. https://www.researchgate.net/publication/298413768_Soybean_glycinin_basic_subunit_inhibits_methicillin_resistant-vancomycin_intermediate_Staphylococcus_aureus_MRSA-VISA_in_vitro

Osman, A., Bin-Jumah, M., Abd El-Hack, M., Elaraby, G., Swelum, A.A., Taha, A.E., Sitohy, M., Allam, A.A & Ashour, E.A. (2020). Dietary supplementation of soybean glycinin can alter the growth, carcases traits, blood biochemical indices, and meat quality of broilers. Poult. Sci, 99:820–828. Doi: https://doi.org/10.1016/j.psj.2019.12.026

Poolman, J.T & Anderson, A.S. (2018). Escherichia coli and Staphylococcus aureus: Leading bacterial pathogens of healthcare associated infections and bacteremia in older-Age populations. Expert Rev. Vaccines, 17:607–618. Doi: https://doi.org/10.1080/14760584.2018.1488590

Ruiz, B., A. Chavez, A. Forero, Y. GarcíaHuante, A. Romero, & M. Sánchez (2010). Production of microbial secondary metabolites: regulation by the carbon source. Crit. Rev. Microbiol, 36: 146–167. Doi: https://doi.org/10.3109/10408410903489576

Simons, A., Alhanout, K. & Duval, R.E. (2020). Bacteriocins, antimicrobial peptides from bacterial origin: overview of their biology and their impact against multidrug-resistant bacteria. Microorganisms 8: 639. Doi: http://doi.org/10.3390/ microorganisms8050639

Sreeramulu, G., Zhu, Y. & Knol, W. (2000). Kombucha fermentation and its antimicrobial activity. Journal of Agricultural and Food Chemistry, 48: 2589–2594. Doi: http://doi.org/10.1021/jf991333m

Tan, W.C., Muhialdin, B.J, & Hussin, A.S.M., (2020). Influence of storage conditions on the quality, metabolites, and biological activity of Soursop (Annona muricata. L.) kombucha. Frontiers in Microbiology, 11: 603481. Doi: http://doi.org/10.3389/fmicb.2020.603481

Vitas, J., Malbasa, R., Grahovac, J., & Loncar, E. (2013). The antioxidant activity of Kombucha fermented milk products with stinging nettle and winter savory. Chemical Industry and Chemical Engineering Quarterly, 19(1):129–139. Doi: https://doi.org/10.1016/j

Verrillo, M., Salzano, M., Cozzolino, V., Spaccini, R. & Piccolo, A., 2021. Bioactivity and antimicrobial properties of chemically characterized compost teas from different green composts. Waste Management 120: 98–107. Doi: http://doi.org/10.1016/j. wasman.2020.11.013

Velićanski, A., Cvetković, D.D., Markov, S.L., Šaponjac, V.T.T. & Vulić, J.J., (2014). Antioxidant and antibacterial activity of the beverage obtained by fermentation of sweetened lemon balm (Melissaofficinalis L.) tea with symbiotic consortium of bacteria and yeast. Food Technology and Biotechnology, 52: 420–429. Doi: http://doi.org/10.17113/b.52.04.14.3611

Vorha, B.M., Shazrul, F., Fareed S., & Othman, B.A. (2019). Effects of Medium Variation and Fermentation Time on the Antiokxidant and Antimicrobial Properties of Kombucha. Malaysian Journal of Fundamental and Applied Sciences. 298-302. Doi: https://doi.org/10.11113/mjfas.v15n2-1.1536

Author Biographies

Putu Rima Sintyadewi, Institut Teknologi dan Kesehatan Bali

Author Origin : Indonesia

Pande P Elza Fitriani, Institut Teknologi dan Kesehatan Bali

Author Origin : Indonesia

Ida Ayu Putu Ary Widnyani, Institut Teknologi dan Kesehatan Bali

Author Origin : Indonesia

Putu Indrayoni, Institut Teknologi dan Kesehatan Bali

Author Origin : Indonesia

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How to Cite

Sintyadewi, P. R. ., Fitriani, P. P. E. ., Widnyani, I. A. P. A. ., & Indrayoni, P. . (2023). Potensi Aktivitas Antibakteri Minuman Fungsional Kombucha Berbahan Dasar Bunga Kecombrang (Etlingera elatior) Berdasarkan Lamanya Waktu Fermentasi. Jurnal Biologi Tropis, 23(1), 403–410. https://doi.org/10.29303/jbt.v23i1.6126

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