An Amperometric Biosensor For Hg(II) Based On Urease/Alginate–Chitosan Membrane Modified Screen Printed Carbon Electrode

Rochmad Kris Sanjaya, Nurul Ismillayli, Dhony Hermanto


A screen-printed three-electrode system is fabricated to prepare a novel screen-printed biosensor for rapid determination of Hg(II) in aqueous solution. The amperometric biosensor is prepared by entrapping urease in alginate–chitosan membrane to modify the screen-printed carbon electrode. The urease/alginate–chitosan membrane for Hg(II) had optimum measurement conditions at work potential of -0.15 V, pH of 7, urea concentration of 75 mM, response time of 8 seconds, inhibition time of 7 minutes and temperature of 25 °C. The resulted biosensor characteristic were found to have the range concentration of Hg(II) ion between 40-90 ppb with the detection limit I10% was 66.45 ppb, the coefficient of variance (Cv) was 0.8%, and reactivation was 5 times reuse.


Amperometric biosensor, Urease/alginate–chitosan membrane, Screen- printed carbon electrode

Full Text:



Szkoda, J., Żmudzki, J. A. N., & Grzebalska, A. (2006). Determination of Total Mercury in Biological Material by Atomic Absorption Spectrometry Method, Mercury, 45, 363–366.

Nixon, D. E., Burritt, M. F., & Moyer, T. P. (1999). The Determination of Mercury in Whole Blood and Urine by Inductively Coupled Plasma Mass Spectrometry, Spectrochim. Acta Part B: Atomic Spec., 54(8), 1141–1153.

Pujol, L., Evrard, D., Groenen-Serrano, K., Freyssinier, M., Ruffien-Cizsak, A., & Gros, P. (2014). Electrochemical Sensors and Devices for Heavy Metals Assay in Water: the French Groups’ Contribution., Front. Chem., 2(4), 1–24.

Elsebai, A. B., Ghica, M. E., Abbas, N., & Brett, C. M. A. (2017). Catalase Based Hydrogen Peroxide Biosensor for Mercury Determination by Inhibition Measurements, J. Hazard. Mater., 340, 344-350.

Turdean, G. L. (2011). Design and evelopment of Biosensors for the Detection of Heavy Metal Toxicity, Int. J. Electrochem., 2011, 1–15.

Krawczynski, T., & Krawczyk, V. (1998). Analytical Applications of Inhibition of Enzymatic Reactions, Chem. Anal., 43, 135-158.

Kuswandi, B. (2003). Simple Optical Fibre Biosensor Based on Immobilised Enzyme for Monitoring of Trace Heavy Metal Ions, Anal. Bioanal. Chem., 376(7), 1104–1110.

Kuralay, F., Ozy, H., & Yıldız, A. (2007). Inhibitive Determination of Hg2+ Ion by An Amperometric Urea Biosensor Using Poly(Vinylferrocenium) Film, Enzym. Microb. Technol., 40, 1156–1159.

Hermanto, D., Mudasir, M., Siswanta, D., & Kuswandi, B. (2019a). Synthesis of Alginate- Chitosan Polyelectrolyte Complex (PEC) Membrane and Its Physical-Mechanical Properties. J. Sci. Appl. Chem., 22(1), 11–16.

Yabuki, S. (2011). Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis, Anal. Sci. 27(7), 695–702.

Hermanto, D., Kuswandi, B., Siswanta, D., & Mudasir, M. (2019b). Inhibitive Determination of Hg(II) in Aqueous Solution Using Urease Amperometric Biosensor. Ind. J. Chem., 19(3), 786–795.

Azmi, N. E., Abdullah, J., Ahmad, M., Sidek, H., Heng, L. Y., & Rahman, S. A. (2012). An Optical Based Biosensor for the Determination of Ammonium in Aqueous Environment, Am. J. Anal. Chem., 3(5), 364–370.

Hermanto, D., Mudasir, M., Siswanta, D., & Kuswandi, B. (2019c). Pemanfaatan Kompleks Polielektrolit sebagai Matriks untuk Imobilisasi Urease dan Aplikasinya sebagai Membran Biosensor Monitor Hg(II), Al Kimia, 7(1), 1-9.

Tsai, H., & Doong, R. (2005). Simultaneous Determination of pH, Urea, Acetylcholine and Heavy Metals Using Array-Based Enzymatic Optical Biosensor, Biosens. Bioelectron., 20,1796–1804.

Lee, S., & Lee, W. (2002). Determination of Heavy Metal Ions Using Conductometric Biosensor Based on Sol-Gel-Immobilized Urease, Bull. Korean Chem. Soc. 23(8), 1169–1172.


  • There are currently no refbacks.