An Amperometric Biosensor For Hg(II) Based On Urease/Alginateââ¬âChitosan Membrane Modified Screen Printed Carbon Electrode
DOI:
10.29303/jpm.v14i3.1338Published:
2019-09-30Issue:
Vol. 14 No. 3 (2019): SeptemberKeywords:
Amperometric biosensor, Urease/alginateââ¬âchitosan membrane, Screen- printed carbon electrodeArticles
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Abstract
A screen-printed three-electrode system is fabricated to prepare a novel screen-printed biosensor for rapid determination of Hg(II) in aqueous solution. The amperometric biosensor is prepared by entrapping urease in alginateââ¬âchitosan membrane to modify the screen-printed carbon electrode. The urease/alginateââ¬âchitosan membrane for Hg(II) had optimum measurement conditions at work potential of -0.15 V, pH of 7, urea concentration of 75 mM, response time of 8 seconds, inhibition time of 7 minutes and temperature of 25 ðC. The resulted biosensor characteristic were found to have the range concentration of Hg(II) ion between 40-90 ppb with the detection limit I10% was 66.45 ppb, the coefficient of variance (Cv) was 0.8%, and reactivation was 5 times reuse.References
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Author Biographies
Rochmad Kris Sanjaya, Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas Kadiri, Kediriââ¬âJawa Timur, Indonesia
Nurul Ismillayli, Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mataram.
Dhony Hermanto, Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mataram.
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