Isolation of piperine from black pepper (Piper nigrum) in the provision of standard compounds for natural chemical practice and research activities
DOI:
10.29303/jpm.v16i5.2981Published:
2021-11-30Issue:
Vol. 16 No. 5 (2021): November 2021Keywords:
Isolation, Black Pepper, Reflux, Recrystallization, Piperine, Standard CompoundArticles
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Abstract
Research conducted to meet the needs of standard compounds in practical and research activities. The use of standard compounds improves the quality of a study. Piperine is the main secondary metabolite compound of the alkaloid group contained in Black PepperàPiper nigrumàL. The isolation method used is reflux and recrystallization methods. The reflux process in absorbing piperine compounds with diethyl ether solvent will help break down the cell walls to absorb the compounds optimally. Recrystallization was repeated 7-8 times until the crystals were pale yellow. The purity test used the TLC test with a solvent ratio of n-hexane and chloroform 6:4 with an Rf value of 0.04 cm. GCMS confirmation of 85% purity, UV-Visible spectroscopic test maximum wavelength 343 with absorbance 0.865 and FTIR test confirmed wave number 928 cm-1 (CO), 996 cm-1 ((CH bending of trans ââ¬âCH=CH-), 1028 cm-1(=COC sym. st.), 1250 cm-1 (=COC asym. st.), 1440 cm-1 (methylenedioxy CH2bending), 1581 cm-1 (arom. C=C st.), 1631 cm-1 (-CO-N-). Based on the confirmed test results, the isolated compound is piperine from the alkaloid group. Piperine compounds can be used as standard compound preparations for testing the purity of compounds in research and chemistry lab work on natural ingredients.References
Putri, I. Z., & Sumarno, S. (2017). Perbedaan Efek Antibakteri Ekstrak Etanol Lada Hitam (Piper Nigrum L.) dengan Ekstrak Etanol Lada Putih (Piper Nigrum L.) terhadap Streptococcus Mutans secara In Vitro. E-Prodenta Journal of Dentistry, 1(1), 1-7.
Vasavirama, K.and Upender, M., 2014, Piperine: A Valuable Alkaloid from Piper Species, International Journal of Pharmacy and Pharmaceutical Sciences, 6 (4): 34-38.
Hakim, A., Loka, I. N., Hajar, S., & Junaidi, E. (2020). Isolation and Confirmation of Quercetin-3-O-Glycosides from Rubber Cassava Leaves. Natural Resources, 11(9), 420-425.
Chithra, S., Jasim, B., Sachidanandan, P., Jyothis, M., & Radhakrishnan, E. K. (2014). Piperine production by endophytic fungus Colletotrichum gloeosporioides isolated from Piper nigrum. Phytomedicine, 21(4), 534-540.
Hakim, A., & Jufri, A. W. (2018). Natural products laboratory project: Isolation and structure elucidation of piperin from piper nigrum and andrographolide from Andrographis paniculata. Journal of Turkish Science Education, 15(4), 15-28.
Subramanian, R., Subbramaniyan, P., Ameen, J. N., & Raj, V. (2016). Double bypasses soxhlet apparatus for extraction of piperine from Piper nigrum. Arabian journal of chemistry, 9, S537-S540.
Mulangsri, D. A. K., Budiarti, A., & Saputri, E. N. (2017). Aktivitas antioksidan fraksi dietileter buah mangga arumanis (Mangifera indica L.) dengan metode DPPH. Jurnal Pharmascience, 4(1).
Kusnadi, K., & Devi, E. T. (2017). Isolasi dan Identifikasi Senyawa Flavanoid pada Ekstrak Daun Seledri (Apium graveolens L.) dengan Metode Refluks. PSEJ (Pancasakti Science Education Journal), 2(1).
Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese medicine, 13(1), 20.
Pramono, A., Fauzantoro, A., Hidayati, I. R., Hygea, A., Puspita, O. S., Muktamiroh, H., ... & Gozan, M. (2018, March). In vitro assay of ethanolic heat reflux extract of Nicotiana tabacum L. var Virginia against nosocomial bacteria pathogen. In Journal of Physics: Conference Series (Vol. 970, No. 1, p. 012021). IOP Publishing.
Purwanto, B. T. (2018). Sintesis Senyawa N-(2-klorobenzoil)-Nââ¬â¢fenilurea dan uji aktivitasnya antikanker terhadap sel HeLa. Jurnal Ilmu Kefarmasian Indonesia, 16(2), 159-165.
Sugiyono. 2017. Statistika Untuk Penelitian. Bandung: Alfabeta.
Hikmawanti, N. P. E., Hariyanti, C. A., & Viransa, V. P. (2016). Kandungan piperin dalam ekstrak buah lada hitam dan buah lada putih (Piper nigrum L.) yang diekstraksi dengan variasi konsentrasi etanol menggunakan metode KLT-densitometri. Media Farmasi, 13(2), 173-185.
Hasriyani, H., Zulfa, A., Anggun, L., & Murhayati, R. (2021). UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70% BIJI LADA HITAM (Piper nigrum L) TERHADAP BAKTERI Escherichia coli. Indonesia Jurnal Farmasi, 5(2), 14-18.
Subramanian, R., Subbramaniyan, P., Ameen, J. N., & Raj, V. (2016). Double bypasses soxhlet apparatus for extraction of piperine from Piper nigrum. Arabian journal of chemistry, 9, S537-S540.
Kolhe, S.R., Borole, P., and Patel, U., 2011, Extraction and Evaluation of Piperine from Piper nigrum, Internasional Journal of Applied Biology and Pharmaceutical Technology, 144-149.
Pratama, D. G. A. Y., Bawa, I. G. A. G., & Gunawan, I. W. G. (2016). Isolasi dan identifikasi senyawa minyak atsiri dari tumbuhan sembukan (Paederia foetida L.) dengan metode kromatografi gas-spektroskopi massa (GC-MS). Jurnal Kimia, 10(1), 149-154.
Fagen, H. J., Kolen, E. P., & Hussong, R. V. (1955). Spice analysis, spectrophotometric method for determining piperine in oleoresins of black pepper. Journal of Agricultural and Food Chemistry, 3(10), 860-862.
Moraru, A. C., ROà žCA, I., CRÃâCIUN, B., Nicolescu, A., Chiriac, A. E., & Voicu, V. (2019). INSIGHTS OF THE ANTIMICROBIAL ACTIVITY OF PIPERINE EXTRACTED FROM PIPER NIGRUM L. Farmacia, 67(6), 1099-1105.
Author Biography
Lalu Sulman, PLP Chemical Education Laboratory FKIP University of Mataram
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