Antioxidant potential of gotu kola leaf extract (Centella asiatica (L.) Urban) as an alternative to antihyperglycemic herbal drinks
DOI:
10.29303/jpm.v17i6.4179Published:
2022-11-30Issue:
Vol. 17 No. 6 (2022): November 2022Keywords:
Centella asiatica (l.) urban, Antioxidant, AntihyperglycemicArticles
Downloads
How to Cite
Downloads
Metrics
Abstract
This study aims to determine the antihyperglycemic potential of Gotu kola leaf extract (Centella asiatica (L.) Urban as an alternative herbal drink in diabetic male white mice. This study was divided into four stages: extracting Gotu kola leaves using 96% ethanol as solvent, phytochemical screening, and testing antihyperglycemic activity. Male white mice were made diabetic by alloxan-induced. Mice were divided into five groups consisting of negative control (Aquadest), positive control (Glibenclamide 13 mg/Kg BW), dose 1 (200 mg Gotu kola extract/kg BW), dose 2 (400 mg Gotu kola extract/kg BW), dose 2 (400 mg Gotu kola extract/kg BW), 3 (600 mg Gotu kola extract/kg BW). The results of phytochemical screening showed that the Gotu kola leaf extract was positive for flavonoids and tannins. The analysis results on the Gotu kola leaf extract showed an IC50 value of 80.58 ppm, including strong antioxidant activity. The results of the antihyperglycemic activity test showed that diabetic male white mice were found to have decreased blood sugar levels on days 14 and 21 by respectively 24.3% and 41.6%; 25.4% and 42.3%; 37.8% and 55.3% after administration of Gotu kola leaf extract at P1, P2, and P3.
References
Boccardi, A. and Shubrook, J.H. (2022). Cutaneous Reactions to Antidiabetic Agents: A Narrative Review. Diabetology, 3(1), pp.97-107.
Agustina, Eva. (2017). Uji Aktivitas Senyawa Antioksidan Dari Ekstrak Daun Tiin (Ficus Carica Linn.) Dengan Pelarut Air, Metanol Dan Campuran Metanol-Air. KLOROFIL Vol. 1 No. 1.
Ali, Muhkti. (2016). Optimasi Pengolahan Teh Kulit Buah Naga (Hylocereus polyrhizus). Agritepa. Vol. II, No.2 hal: 216-223.
Anjani, P.P., Andrianty, S., Widyaningsih, T.D. (2015). Pengaruh Penambahan Pandan Wangi dan Kayu Manis pada Teh Herbal Kulit Salak bagi Penderita Diabetes. Jurnal Pangan dan Agroindustri. 3(1): 203-214.
Chari, M. G., & Colagar, A. H. (2011). Seminal plasma lipid peroxidation, total antioxidant capacity, and cigarette smoking in asthenoteratospermic men. Journal of Men’s Health, 8(1), 43–49.
Eguchi, N., Toribio, A.J., Alexander, M., Xu, I., Whaley, D.L., Hernandez, L.F., Dafoe, D. and Ichii, H. (2022). Dysregulation of β-Cell Proliferation in Diabetes: Possibilities of Combination Therapy in the Development of a Comprehensive Treatment. Biomedicines, 10(2), p.472.
Forkink, M., Smeitink, J. a M., Brock, R., Willems, P. H. G. M., & Koopman, W. J. H. (2010). Detection and manipulation of mitochondrial reactive oxygen species in mammalian cells. Biochimica et Biophysica Acta, 1797(6-7), 1034–44.
Gülçin, İ. (2012). Antioxidant activity of food constituents: an overview. Archives of Toxicology, 86(3), 345–91.
N. K. Trisna Rahayu, I. D. G. Mayun Permana, and G. K. Diah Puspawati (2020). Pengaruh Waktu Maserasi Terhadap Aktivitas Antioksidan Ekstrak Daun Gotu kola (Centella asiatica (L.) Urban). J. Ilmu dan Teknologi Pangan. 7(2)
Kusumowati, Ika T D. dan Sudjono, T A; dkk. (2012). “Korelasi Kandungan Fenolik Dan Aktivitas Antiradikal Ekstrak Etanol daun Empat Tanaman Obat Indonesia (Piper bettle, Sauropus androgynus, Averrhoa bilimbi, Guazuma ulmifolia). Jurnal Farmasi Indonesia. 13(1).
Hajleh, M.N.A., Khleifat, K.M., Alqaraleh, M., AlHraishat, E., Al-limoun, M.O., Qaralleh, H. and AlDujaili, E.A. (2022). Antioxidant and Antihyperglycemic Effects of Ephedra foeminea Aqueous Extract in Streptozotocin-Induced Diabetic Rats. Nutrients, 14(11), p.2338.
Khalid, M., Petroianu, G. and Adem, A. (2022). Advanced Glycation End Products and Diabetes Mellitus: Mechanisms and Perspectives. Biomolecules, 12(4), p.542.
Kusumaningrum, Ria; Supriadi, Agus dan J, Siti, H R. (2013). “Karakteristik Dan Mutu Teh Bunga Lotus (Nelumbo nucifera)”. Fishtech. 2(1).
Soumyanath A, Zhong YP, Henson E, Wadsworth T, Bishop J, Gold BG, et. al. (2012). Centella asiatica extract improves behavioral deficits in a mouse model of alzheimer’s disease: investigation of a possible mechanism of action. Int J Alzheimers Dis.
Kifle, Z.D., Abdelwuhab, M., Melak, A.D., Meseret, T. and Adugna, M. (2022). Pharmacological evaluation of medicinal plants with antidiabetic activities in Ethiopia: A review. Metabolism Open, p.100174.
Lamkeng, S., Santibenchakul, S. and Sooksawat, N. (2022). Potential of Maranta arundinacea residues for recycling: Analysis of total phenolic, flavonoid, and tannin contents. Biodiversitas Journal of Biological Diversity, 23(3).
Méril-Mamert, V., Ponce-Mora, A., Sylvestre, M., Lawrence, G., Bejarano, E. and Cebrián-Torrejón, G. (2022). Antidiabetic Potential of Plants from the Caribbean Basin. Plants, 11(10), p.1360.
Gallego, Ana., Karla Ramirez-Estrada, Heriberto Rafael Vidal-Limon, Diego Hidalgo, Liliana Lalaleo, Waqas Khan Kayani, Rosa M. Cusido, Javier Palazon. 2014. Biotechnological Production of Centellosides in Cell Cultures of Centella asiatica (L) Urban. Eng. Life Sci, 14: 633–642.
Sisein, E. A. (2014). Review Article Biochemistry of Free Radicals and Antioxidants, 2(2), 110–118.
Soa UOM, Amelia R, Octaviani DA. (2018). Perbandingan Efektivitas Pemberian Rebusan Jahe Merah Dan Daun Mint Dengan Jeruk Nipis Dan Madu Terhadap Mual Muntah Pada Ibu Hamil Trimester I Di Puskesmas Waepana, Ngada, Ntt. Jurnal Kebidanan. 8(2):157
Widyani, M., Ulfa, M., & Wirasisya, D. G. (2019). Efek Penghambatan Radikal Bebas Infusa dan Ekstrak Etanol Herba Pegagan (Centella Asiatica (L.) Urb) Dengan Metode DPPH. Jurnal Pijar Mipa, 14(1), 100-106.
Nofita, S. D., Ngibad, K., & Rodli, A. F. (2022). Determination of percentage yield and total phenolic content of ethanol extract from purple passion (Passiflora edulis f. edulis Sims) fruit peel. Jurnal Pijar Mipa, 17(3), 309-313.
Aisyah, S. D., & Ngibad, K. (2022). Determination of flavonoid content of ethanol and ethyl acetate extract from purple passion fruit peel. Jurnal Pijar Mipa, 17(5), 696-700.
Sutrisno, E., Adnyana, I.K., Sukandar, E.Y., Fidrianny, I., Lestari, T. (2014) Kajian Aktivitas Penyembuhan Luka Dan Antibakteri Binahong (Anredera cordifolia (Ten.) Steenis, Gotu kola (Centella Asiatica (L.) Urban) Serta Kombinasinya Terhadap Bakteri Staphylococcus Aureus Dan Pseudomonas Aeruginosa Dari Pasien Luka Kaki Diabetes. Bionatura-Jurnal Ilmu-ilmu Hayati dan Fisik, 16(2),78 – 82.
Author Biographies
Ida Ayu Manik Damayanti, Clinical and Community Pharmacy Study Program Faculty of Health, Institute of Technology and Health Bali
Ni Wayan Sukma Antari, Nursing Study Program, Faculty of Health, Institute of Technology and Health Bali
Sri Dewi Megayanti, Nursing Study Program, Faculty of Health, Institute of Technology and Health Bali
License
Copyright (c) 2022 Ida Ayu Manik Damayanti, Ni Wayan Sukma Antari, Sri Dewi Megayanti
This work is licensed under a Creative Commons Attribution 4.0 International License.
The following terms apply to authors who publish in this journal:
1. Authors retain copyright and grant the journal first publication rights, with the work simultaneously licensed under a Creative Commons Attribution License 4.0 International License (CC-BY License) that allows others to share the work with an acknowledgment of the work's authorship and first publication in this journal.
2. Authors may enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), acknowledging its initial publication in this journal.
3. Before and during the submission process, authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website), as this can lead to productive exchanges as well as earlier and greater citation of published work (See The Effect of Open Access).
