Vol. 26 No. 1 (2026): Januari-Maret
Open Access
Peer Reviewed

Literature Review: The Role of Ascorbic Acid as a Priming Compound in the Physiological Response of Plants to Drought Stress

Authors

Muftia Nadhra , Anggraini Anggraini , Violita Violita

DOI:

10.29303/jbt.v26i1.11463

Published:

2026-03-31

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Abstract

Drought stress is a major abiotic factor that limits plant growth and productivity by disrupting photosynthesis, water uptake, and cellular metabolism. One strategy to enhance plant tolerance to drought is seed priming using ascorbic acid (AsA), which functions as an important antioxidant in plant defense systems. This study aims to systematically review the role of AsA as a priming compound in improving plant physiological responses under drought stress. A systematic literature review was conducted following the PRISMA framework. Relevant articles were collected from Google Scholar, ScienceDirect, and MDPI databases using keywords related to drought stress, seed priming, and ascorbic acid. Selected studies were analyzed descriptively to identify patterns of plant responses. The results indicate that AsA priming enhances antioxidant enzyme activities, reduces reactive oxygen species accumulation, maintains membrane stability, and improves germination, photosynthesis, and early seedling growth across several crop species. In conclusion, AsA seed priming is an effective approach to improve plant tolerance to drought stress. Future studies are recommended to explore optimal concentrations and molecular mechanisms of AsA application in different crop species.

Keywords:

Ascorbic acid, drought, plant, priming, oxidative stress.

References

Abubakar, M. A., Yusuf, M., Lawal, A. A., & Sadiq, I. S. (2024). Synergistic effects of ascorbic acid and microwave seed priming on growth, chlorophyll content, and stress tolerance of spinach (Spinacia oleracea) under drought conditions. Journal of Plant Stress Physiology, 15(2), 101–110.

Alam, M. M., Hasanuzzaman, M., Nahar, K., & Fujita, M. (2023). Exogenous ascorbic acid enhances drought tolerance by regulating antioxidant defense and osmolyte accumulation in plants. Plant Stress, 7, 100147. https://doi.org/10.1016/j.stress.2022.100147

Ali, S., Rizwan, M., Hussain, A., Rehman, M. Z. U., Ali, B., Yousaf, B., & Ahmad, P. (2021). Ascorbic acid mitigates drought stress in plants: Physiological and molecular insights. Journal of Plant Growth Regulation, 40(3), 1385–1400. https://doi.org/10.1007/s00344-020-10158-9

Al-Demrdash, F. M., El-Sabagh, A., Hossain, A., & Barutçular, C. (2025). Role of antioxidants in improving plant tolerance to drought stress. Agronomy, 15(1), 112. https://doi.org/10.3390/agronomy15010112

Anjum, S. A., Tanveer, M., Ashraf, U., Hussain, S., Shahzad, B., Khan, I., & Wang, L. (2021). Osmoregulation and antioxidant production in plants under drought stress. Plants, 10(2), 246. https://doi.org/10.3390/plants10020246

Flexas, J., Diaz-Espejo, A., Gago, J., Gallé, A., Galmés, J., Gulías, J., & Medrano, H. (2014). Photosynthetic limitations in Mediterranean plants under drought stress. Plant, Cell & Environment, 37(6), 1274–1287. https://doi.org/10.1111/pce.12241

Violita, V., & Hamim, H. (2010). Sistem pertahanan tanaman kedelai yang mendapat perlakuan cekaman kekeringan. Eksakta, 2.

Hamim, H., Violita, V., Triadiati, T., & Miftahudin, M. (2017). Oxidative stress and photosynthesis reduction of cultivated (Glycine max L.) and wild soybean (G. tomentella L.) exposed to drought and paraquat. Asian Journal of Plant Sciences, 16(2), 65–77.

Han, Y. (2025). Seed priming with ascorbic acid enhances antioxidant defense and early seedling tolerance of cotton (Gossypium hirsutum) under abiotic stress. Plant Stress, 7, 100145. https://doi.org/10.1016/j.stress.2024.100145

Khamis, M. H., Hassan, F. A., & Seleiman, M. F. (2025). Drought stress effects on crop productivity and mitigation strategies. Frontiers in Plant Science, 15, 1298743. https://doi.org/10.3389/fpls.2024.1298743

Khazaei, H. R., Estaji, A., & Niknam, V. (2019). Effect of seed priming with ascorbic acid on antioxidant enzymes activity and lipid peroxidation of pepper (Capsicum annuum) seedlings under drought stress. Scientia Horticulturae, 246, 395–401. https://doi.org/10.1016/j.scienta.2018.11.025

Maaroufi-Dguimi, H., Ben Abdallah, F., & Bouaziz, M. (2023). Ascorbic acid seed priming improves germination and reduces oxidative stress in tomato (Solanum lycopersicum) under osmotic stress. South African Journal of Botany, 156, 223–231. https://doi.org/10.1016/j.sajb.2023.01.014

Mi, J., Liu, H., Zhang, Y., & Wang, Q. (2025). Seed priming with ascorbic acid improves seed viability and membrane stability of oat (Avena sativa) under drought stress. Seed Science and Technology, 53(1), 45–56.

Mittler, R. (2017). ROS are good. Trends in Plant Science, 22(1), 11–19. https://doi.org/10.1016/j.tplants.2016.08.002

Nyaupane, D. (2024). Plant growth and productivity under water deficit conditions. Plant Physiology Reports, 29(1), 1–12. https://doi.org/10.1007/s40502-023-00729-6

Paparella, S., Araujo, S. S., Rossi, G., Wijayasinghe, M., Carbonera, D., & Balestrazzi, A. (2022). Seed priming: State of the art and new perspectives. Plant Cell Reports, 41(5), 993–1011. https://doi.org/10.1007/s00299-021-02796-5

Raut, V. V., Bapat, V. A., & Yadav, S. R. (2025). Ascorbic acid-mediated stress tolerance in plants. Plant Physiology and Biochemistry, 208, 108560. https://doi.org/10.1016/j.plaphy.2024.108560

Rosawanti, P., Ghulamahdi, M., & Khumaida, N. (2015). Respon anatomi dan fisiologi akar kedelai terhadap cekaman kekeringan. Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 43(3), 186–192. https://doi.org/10.24831/jai.v43i3.11243

Sharma, P., Jha, A. B., Dubey, R. S., & Pessarakli, M. (2024). Ascorbate metabolism and drought stress tolerance in plants. Physiologia Plantarum, 176(2), e14015. https://doi.org/10.1111/ppl.14015

Srivastava, A. K. (2020). Ascorbic acid: An essential plant antioxidant. Biologia Plantarum, 64(1), 1–11. https://doi.org/10.32615/bp.2020.001

Taiz, L., Zeiger, E., Møller, I. M., & Murphy, A. (2015). Plant physiology and development (6th ed.). Sinauer Associates.

Tasan, S., Kaya, C., & Ashraf, M. (2024). Ascorbic acid seed priming enhances germination and early seedling growth of canola (Brassica napus) under osmotic stress. Journal of Plant Nutrition, 47(4), 512–523. https://doi.org/10.1080/01904167.2023.2278901

Ur Rahman, M., Gul, S., Ahmad, I., & Khan, A. (2021). Seed priming with ascorbic acid improves water relations, photosynthesis, and biomass production of wheat (Triticum aestivum) under drought stress. Physiology and Molecular Biology of Plants, 27(5), 1021–1031. https://doi.org/10.1007/s12298-021-00980-3

Zagoto, A. D. P., & Violita, V. (2019). Leaf anatomical modification in rice drought. Eksakta, 20(2), 42–52. https://doi.org/10.24036/eksakta/vol20-iss2/176

Zhang, L., Chen, Y., Wang, M., & Li, Z. (2024). Ascorbic acid seed priming enhances root growth and auxin-related gene expression in rice (Oryza sativa L.) under drought stress. Plant Physiology and Biochemistry, 201, 107921. https://doi.org/10.1016/j.plaphy.2023.107921

Zhang, Y., Liu, X., Li, Y., & Wang, J. (2024). Ascorbic acid regulates antioxidant enzymes under drought stress. Plant Physiology and Biochemistry, 203, 108201. https://doi.org/10.1016/j.plaphy.2023.108201

Zulfiqar, F., Ashraf, M., & Siddique, K. H. M. (2023). Seed priming improves drought tolerance in crops. Agronomy, 13(2), 486. https://doi.org/10.3390/agronomy13020486

Author Biographies

Muftia Nadhra, Universitas Negeri Padang

Author Origin : Indonesia

Anggraini Anggraini, Universitas Negeri Padang

Author Origin : Indonesia

Violita Violita , Universitas Negeri Padang

Author Origin : Indonesia

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How to Cite

Nadhra, M., Anggraini, A., & Violita , V. (2026). Literature Review: The Role of Ascorbic Acid as a Priming Compound in the Physiological Response of Plants to Drought Stress. Jurnal Biologi Tropis, 26(1), 1128–1134. https://doi.org/10.29303/jbt.v26i1.11463

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