Pengaruh Priming Auksin Terhadap Perkecambahan Benih Padi Ir64 Pada Cekaman Salinitas
DOI:
10.29303/jbt.v26i1.11474Published:
2026-03-31Downloads
Abstract
Salinity is a major abiotic constraint that limits rice establishment, particularly during germination when seedlings are physiologically vulnerable. This study investigated the effect of auxin priming on germination performance and early seedling growth of IR64 rice exposed to 100 mM NaCl, and identified the most effective auxin concentration. The experiment was conducted using a Completely Randomized Design with five treatments: non-primed seeds and auxin priming at concentrations of 0, 25, 50, and 100 ppm, each with three replications. Statistical analysis was performed using analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test at the 5% significance level. Parameters observed included germination percentage and rate, root and plumule length, total seedling length, vigor index, fresh and dry weight, and lipid peroxidation. Auxin priming significantly enhanced germination rate, seedling growth, vigor index, and biomass accumulation, while reducing lipid peroxidation under saline conditions. Among the treatments, P3 exhibited the most consistent improvement under salinity stress, whereas P1 showed the highest overall growth under non-stress conditions. These findings indicate that appropriate auxin priming can improve the early physiological performance of rice seedlings under salt stress.
Keywords:
Auxin, oxidative stress, rice, seed priming, salinityReferences
Abdul-Baki, A. A., & Anderson, J. D. (1973). Vigor determination in soybean seed by multiple criteria. Crop Science, 13(6), 630–633. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
Abo-El-Kheer, E. S. A., Gad, S. B., & Abido, W. A. E. (2019). The integrate effect of seed soaking in aqueous plant extracts and ascorbic acid on germination and seedling characters of rice grains under salinity stress. Journal of Plant Production, 10(7), 511-514. https://doi.org/10.21608/jpp.2019.53545
Acosta‑Motos, J. R., Ortuño, M. F., Bernal‑Vicente, A., Diaz‑Vivancos, P., Sanchez‑Blanco, M. J., & Hernandez, J. A. (2017). Plant responses to salt stress: Adaptive mechanisms. Agronomy, 7(1), 18. https://doi.org/10.3390/agronomy7010018
Anjum, S. A., Khan, N. A., & Masood, A. (2014). Ascorbate–glutathione pathway and plant tolerance to abiotic stresses. In A.
Ahmad, S. A. Anjum & S. H. T. Naeem (Eds.), Plant Physiology and Biochemistry (pp. 209–231). Academic Press.
Ballo, M., Nio, S. A., Mantiri, F. R., & Pandiangan, D. (2012). Respons morfologis beberapa varietas padi (Oryza sativa L.) terhadap kekeringan pada fase perkecambahan. Jurnal Bios Logos, 4(2). https://ejournal.unsrat.ac.id/index.php/bioslogos
Bewley, J. D., Bradford, K. J., Hilhorst, H. W. M., & Nonogaki, H. (2013). Seeds: Physiology of development, germination and dormancy (3rd ed.). Springer. https://doi.org/10.1007/978-1-4614-4693-4
Cackett, L., Rountree, K., & Gifford, M. L. (2022). Auxin signaling and abiotic stress responses in plants. Journal of Experimental Botany, 73(18), 6055–6070. https://doi.org/10.1093/jxb/erac300
Fahad, S., Bano, A., Cui, K., & Huang, J. (2021). Hormonal regulation of plant responses to salinity stress. Plant Physiology and Biochemistry, 168, 204–217. https://doi.org/10.1016/j.plaphy.2021.10.003
Foti, S., De Lacerda, C. F., & Bose, J. (2020). Effects of salinity stress on plant growth, seed germination and physiological functions. South African Journal of Botany, 139, 399–408. https://doi.org/10.1016/j.sajb.2021.03.019
Gamalero, F., & Glick, B. R. (2022). Auxin and plant stress responses: From signaling to tolerance. Journal of Experimental Botany, 73(22), 7000–7014. https://doi.org/10.1093/jxb/erac358
Ghiyasi, M., Amirnia, R., Fazelimanesh, M., & Tajbakhsh, M. (2016). Seed priming with ascorbic acid under saline conditions. Thai Journal of Agricultural Science, 48(4), 179–185. https://www.thaiscience.info/journals/Article/TJAS/10973747.pdf
Gill, S. S., & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48(12), 909–930. https://doi.org/10.1016/j.plaphy.2010.08.016
Hasanuzzaman, M., Nahar, K., Alam, M. M., & Fujita, M. (2022). Plant responses and tolerance to salt stress. Springer. https://doi.org/10.1007/978-981-16-4362-9
Hasanuzzaman, M., Bhuyan, M. H. M. B., Zulfiqar, F., Raza, A., Mohsin, S. M., Mahmud, J. A., Fujita, M., & Fotopoulos, V. (2020). Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator. Antioxidants, 9(8), 681. https://doi.org/10.3390/antiox9080681
Hassan, N. E. (2024). Salinity stress in plants: Growth, photosynthesis and adaptation review. GSC Advanced Research and Reviews, 20(02), 231–243. https://doi.org/10.30574/gscarr.2024.20.2.0304
Hussain, S., Shaukat, M., Ashraf, M., & Zhu, C. (2021). Salinity stress in rice: Effects, tolerance mechanisms, and management. Plants, 10(6), 1–22. https://doi.org/10.3390/plants10061168
Ismail, A. M., Horie, T., & Nguyên, H. T. (2020). Physiological and genetic basis of salinity tolerance in rice. Plant Molecular Biology, 103(1–2), 1–19. https://doi.org/10.1007/s11103-020-00993-7
Iqbal, M., Ashraf, M., Jamil, A., & Rehman, S. (2016). Does seed priming induce changes in the levels of some endogenous plant hormones in hexaploid wheat plants under salt stress? Journal of Integrative Agriculture, 15(7), 1560–1571. https://doi.org/10.1016/S2095-3119(15)61290-6
Kader, M. A. (2005). A comparison of seed germination calculation formulae. Journal and Proceedings of the Royal Society of New South Wales, 138(3–4), 65–75. https://royalsoc.org.au
Kaur, H., Sirhindi, G., & Bhardwaj, R. (2024). Role of auxin in plant adaptation to salinity stress. Plant Stress, 9, 100199. https://doi.org/10.1016/j.stress.2024.100199
Mittler, R. (2017). ROS are good. Trends in Plant Science, 22(1), 11–19. https://doi.org/10.1016/j.tplants.2016.08.002
Munns, R., & Tester, M. (2020). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 71, 699–728. https://doi.org/10.1146/annurev-arplant-050718-100005
Nour, M. M., Aljabi, H. R., AL-Huqail, A. A., Horneburg, B., Mohammed, A. E., & Alotaibi, M. O. (2024). Drought responses in plants. Frontiers in Ecology and Evolution, 12, 1–14. https://doi.org/10.3389/fevo.2024.1301521
Nonogaki, H., Bassel, G. W., & Bewley, J. D. (2010). Germination—Still a mystery. Plant Science, 179, 574–581. https://doi.org/10.1016/j.plantsci.2010.02.010
Paparella, S., Araújo, S. S., Rossi, G., Wijayasinghe, M., Carbonera, D., & Balestrazzi, A. (2021). Seed priming: State of the art. Plant Cell Reports, 40(2), 231–250. https://doi.org/10.1007/s00299-020-02602-3
Per, T. S., Khan, N. A., Reddy, P. S., Masood, A., Hasanuzzaman, M., Khan, M. I. R., & Anjum, N. A. (2017). Approaches in modulating proline metabolism in plants for salt and drought stress tolerance: Phytohormones, mineral nutrients and transgenics. Plant Physiology and Biochemistry, 115, 126–140. https://doi.org/10.1016/j.plaphy.2017.03.018
Rhaman, M. S., Imran, S., Rauf, F., Khatun, M., & Ahmad, M. (2022). Seed priming improves salt tolerance in rice. Plants, 11(3), 1–16. https://doi.org/10.3390/plants11030317
Rosawanti, P, M Ghulamahdi, & N Khumaida. (2015). Respon Anatomi Dan Fisiologi Akar Kedelai Terhadap Cekaman Kekeringan. J.Agron.Indonesia 43(3): 186–92.
Ryu, H., Cho, Y. G., & Yoon, J. S. (2014). Auxin-mediated regulation of root growth and biomass accumulation in rice under salinity stress. Plant Growth Regulation, 74(2), 189–200. https://doi.org/10.1007/s10725-014-9918-5
Shahid, S. A., Zaman, M., & Heng, L. (2020). Soil salinity: Historical perspectives and a world overview of the problem. FAO Soils Bulletin. Food and Agriculture Organization of the United Nations.
Taiz, L., Zeiger, E., Møller, I. M., & Murphy, A. (2015). Plant physiology and development (6th ed.). Sinauer Associates.
Utama, Y. P., & Violita, V. (2024). Pengaruh Konsentrasi Penyemprotan Auksin Pada Padi (Oryza Sativa) Yang Diberikan Simulasi Cekaman Salinitas. Jurnal Biologi UNAND, 12(2), 149-155.
Wang, Y., Li, K., Li, X., & Chen, X. (2015). Auxin redistribution modulates plastic development of root system architecture under salt stress in rice (Oryza sativa L.). Plant Cell Reports, 34(6), 1015–1028. https://doi.org/10.1007/s00299-015-1765-1
Wang, X., Liu, H., Yu, F., Hu, B., Jia, Y., Sha, H., & Zhao, H. (2019). Antioxidant defense in rice under stress. Scientific Reports, 9(1), 1–11. https://doi.org/10.1038/s41598-019-43861-1
Yan, M., Xiong, Y., Shi, P., Deng, S., Zhao, B., & Chen, X. (2023). Salinity stress inhibits seed germination and seedling growth by affecting osmotic balance and ion homeostasis in rice. Plants, 12(4), 812.
https://doi.org/10.3390/plants12040812
Zagoto, A. D. P., & Violita, V. (2019). Leaf anatomical modification in rice drought. Eksakta, 20(2), 42–52. https://doi.org/10.24036/eksakta/vol20-iss2/176
Zheng, C., Chen, J. P., Wang, X. W., & Li, P. (2025). Reactive oxygen species in plants. Antioxidants, 14(6), 1–17. https://doi.org/10.3390/antiox14060552
Zhou, S., Zhang, B., & Wang, X. (2018). Antioxidant defenses in rice. Rice Science, 25(5), 232–248. https://doi.org/10.1016/j.rsci.2018.06.004
Zhu, L., Zhang, H., & Liu, S. (2024). Oxidative stress in rice under abiotic stresses. Frontiers in Plant Science, 15, 237. https://doi.org/10.3389/fpls.2024.1287237
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