Vol. 26 No. 2 (2026): April - Juni
Open Access
Peer Reviewed

Blue Light Exposure Duration Improves Antioxidant Capacity in Mung Bean Seedlings (Vigna radiata L.)

Authors

Kaylee Ann Siswanto , Artadana Made Ida Bagus

DOI:

10.29303/jbt.v26i2.11604

Published:

2026-05-11

Downloads

Abstract

Antioxidant chemicals found in mung bean (Vigna radiata L.) sprouts may help prevent non-communicable diseases linked to oxidative stress; however, it is unknown how long exposure to blue light affects the build-up of these bioactive substances. This study aimed to evaluate the effect of different duration of blue LED light exposure on growth, antioxidant activity, and antioxidant-related compound in mung bean sprout. Three replications of a completely randomized design (CRD) were used. The Folin-Ciocalteu method was used to measure total phenolic content, iodometric titration was used to measure vitamin C, and the DPPH assay was used to measure antioxidant activity. According to the results, antioxidant activity increased significantly (p<0.05) under 24-hour exposure to blue light (30.34±0.61%) compared to darkness (26.40±0.39%). Additionally, tests made using the Folin-Ciocalteu technique and iodometric titration show an increasing tendency when exposed to blue light. Dry weight and hypocotyl length were not considerably impacted by light treatment. These results suggest that blue light duration is important for boosting antioxidant capacity without sacrificing development. This discovery offers a workable method for enhancing the functional quality of mung bean sprouts for controlled-environment agriculture systems and functional food development.

Keywords:

Antioxidant Blue light Light duration Mung bean sprout

References

Badan Pusat Statistik. (2025). Rata-rata konsumsi per kapita seminggu menurut kelompok sayur-sayuran per kabupaten/kota. https://www.bps.go.id/id/statistics-table/2/MjEwMCMy/ratarata-konsumsi-perkapita-seminggumenurut-kelompok-sayur-sayuranper-kabupaten-kota.html

Bian, Z. H., Yang, Q. C., & Liu, W. K. (2015). Effects of light quality on the accumulation of phytochemicals in vegetables produced in controlled environments: A review. Journal of the Science of Food and Agriculture, 95, 869–877. https://doi.org/10.1002/jsfa.6789

Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1200. https://doi.org/10.1038/1811199a0

Bungala, L. T. D. C., Park, S. U., Nguyen, B. V., Lim, J., Kim, K., Kim, J. K., Park, C. H., Le, A. T., Chung, Y. S., & Yeo, H. J. (2024). Effect of LED lights on secondary metabolites and antioxidant activities in red pakchoi baby leaves. ACS Omega, 9(22), 23420–23430. https://doi.org/10.1021/acsomega.3c10261

Carvalho, S. D., & Folta, K. M. (2014). Sequential light programs shape kale (Brassica napus) sprout appearance and alter metabolic and nutrient content. Horticulture Research, 1, Article 8. https://doi.org/10.1038/hortres.2014.8

Chandimali, N., Bak, S. G., Park, E. H., Lim, H. J., Won, Y. S., Kim, E. K., Park, S. I., & Lee, S. J. (2025). Free radicals and their impact on health and antioxidant defenses: A review. Journal of Biomedical Science, 32(1), 45–58. DOI: 10.1038/s41420-024-02278-8

Chutimanukul, P., Wanchichanun, P., Janta, S., Toojinda, T., Kerdsuwan, O., & Ruenkrairaksa, C. (2023). The influence of different light spectra on physiological responses, antioxidant capacity and chemical compositions in two holy basil cultivars. Scientific Reports, 13, Article 12538. https://doi.org/10.1038/s41598-023-40577-x

Ding, S., Su, P., Wang, D., Chen, X., & Tang, C. (2023). Blue and red-light proportion affects growth, nutritional composition, antioxidant properties and volatile compounds of Toona sinensis sprouts. LWT – Food Science and Technology, 173, Article 114400. https://doi.org/10.1016/j.lwt.2022.114400

Ebert, A. W. (2022). Sprouts and microgreens—Novel food sources for healthy diets. Plants, 11(4), Article 571. https://doi.org/10.3390/plants11040571

Facinelli, B., Bulgari, R., Nicola, S., & Incrocci, L. (2024). The effect of blue:red light proportion on germination parameters, growth attributes, and quality of borage sprouts. Scientia Horticulturae, 336, Article 113399. https://doi.org/10.1016/j.scienta.2024.113399

Georgescu, C. V., Gavat, C. C., & Voinescu, D. C. (2019). Iodometric quantitative analysis method of ascorbic acid in tablets. Revista de Chimie, 70(10), 3555–3560.

Karaman, R., Akgün, İ., & Türkay, C. (2024). Nutritional, phenol content and antioxidant activity of edible sprouts of commonly occurring plants. Vegetos. https://doi.org/10.1007/s42535-024-00818-2

Kementerian Kesehatan Republik Indonesia. (2025). Benarkah dengan skrining kesehatan dapat mencegah kematian dini? https://keslan.kemkes.go.id/view_artikel/4067/benarkah-

Lee, W. H., Zebro, M., & Heo, J. Y. (2023). Light-emitting diode light quality influences germination and sprout characteristics of motherwort. Journal of Food Quality, 4646078. https://doi.org/10.1155/2023/4646078

Lim, Y. J., Kwon, S. J., & Eom, S. H. (2023). Red and blue light-specific metabolic changes in soybean seedlings. Frontiers in Plant Science, 14, Article 1128001. https://doi.org/10.3389/fpls.2023.1128001

Liu, H. K., Chen, Y. Y., Hu, T. T., Zhang, S. J., Zhang, Y. H., Zhao, T. Y., Yu, H. E., & Kang, Y. F. (2016). The influence of light-emitting diodes on the phenolic compounds and antioxidant activities in pea sprouts. Journal of Functional Foods, 25, 459–465. https://doi.org/10.1016/j.jff.2016.06.028

Ma, D., Xu, X., Guo, Y., Fang, J., Yan, C., Noel, J. P., & Liu, H. (2016). Cryptochrome 1 interacts with PIF4 to regulate high temperature-mediated hypocotyl elongation in response to blue light. Proceedings of the National Academy of Sciences, 113(1), 224–229. https://doi.org/10.1073/pnas.1511473113

Mastropasqua, L., Dipierro, N., & Paciolla, C. (2020). Effects of darkness and light spectra on nutrients and pigments in radish, soybean, mung bean and pumpkin sprouts. Antioxidants, 9(6), Article 558. https://doi.org/10.3390/antiox9060558

Mendes, F. Q., Carvalho, R. F., Souza, M. O., & Filho, A. B. C. (2025). Biofortification of arugula microgreens through supplemental blue light. Horticulturae, 11(4), Article 412. https://doi.org/10.3390/horticulturae11040412

Nam, T. G., Kim, D. O., & Eom, S. H. (2018). Effects of light sources on major flavonoids and antioxidant activity in common buckwheat sprouts. Food Science and Biotechnology, 27(1), 169–176. https://doi.org/10.1007/s10068-017-0204-1

Nediani, C., & Giovannelli, L. (2020). Oxidative stress and inflammation as targets for novel preventive and therapeutic approaches in non-communicable diseases. Antioxidants, 9(4), Article 290. https://doi.org/10.3390/antiox9040290

Paradiso, R., & Proietti, S. (2021). Light-quality manipulation to control plant growth and photomorphogenesis in greenhouse horticulture: The state of the art and the opportunities of modern LED systems. Journal of Plant Growth Regulation, 41, 742–780. https://doi.org/10.1007/s00344-021-10337-y

Qian, H. M., Liu, T. Y., Deng, M. D., Miao, H. Y., Cai, C. X., Shen, W. S., & Wang, Q. M. (2016). Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts. Food Chemistry, 196, 1232–1238. https://doi.org/10.1016/j.foodchem.2015.10.055

Sequeros, T., et al. (2021). Mungbean in Southeast Asia and East Africa: Varieties, practices and constraints. Agricultural & Food Security, 10, Article 2. https://doi.org/10.1186/s40066-020-00273-7

Siddiqui, N., Rauf, A., Latif, A., & Mahmood, Z. (2017). Spectrophotometric determination of the total phenolic content. Journal of Taibah University Medical Sciences, 12(4), 360–363. https://doi.org/10.1016/j.jtumed.2016.11.006

Siriparu, P., Panyatip, P., Pota, T., Ratha, J., Yongram, C., Srisongkram, T., ... & Puthongking, P. (2022). Effect of germination and illumination on melatonin and its metabolites, phenolic content, and antioxidant activity in mung bean sprouts. Plants, 11(21), 2990. https://doi.org/10.3390/plants11212990

Sun, K., Peng, Y., Wang, M., Li, W., Li, Y., & Chen, J. (2024). Effect of red and blue light on the growth and antioxidant activity of alfalfa sprouts. Horticulturae, 10(6), 706. https://doi.org/10.3390/horticulturae10060706

Taiz, L., Zeiger, E., Møller, I. M., & Murphy, A. (2015). Plant physiology and development (6th ed.). Sinauer Associates.

World Health Organization. (2023). World health statistics 2023: Monitoring health for the SDGs. https://cdn.who.int/

Wu, W., Wu, H., Liang, R., Huang, S., Meng, L., Zhang, M., ... & Zhu, H. (2025). Light regulates the synthesis and accumulation of plant secondary metabolites. Frontiers in Plant Science, 16, 1644472. https://doi.org/10.3389/fpls.2025.1644472

Zhang, S., Guo, X., Li, J., Zhang, Y., Yang, Y., Zheng, W., & Xue, X. (2022). Effects of light-emitting diode spectral combinations on growth and quality of pea sprouts under long photoperiod. Frontiers in Plant Science, 13, 978462. https://doi.org/10.3389/fpls.2022.978462

Zhang, X., Bian, Z., Yuan, X., Chen, X., & Lu, C. (2020). A review on the effects of light-emitting diode (LED) light on the nutrients of sprouts and microgreens. Trends in food science & technology, 99, 203-216. https://doi.org/10.1016/j.tifs.2020.02.031

Zhao, T., Nie, J., Yan, X., & Xue, W. (2024). Identifying the critical LED light condition.. Scientia Horticulturae, 327, Article 112801. https://doi.org/10.1016/j.scienta.2023.112801

Zheng, Y. J., Zhang, Y. T., Liu, H. C., Li, Y. M., Liu, Y. L., Hao, Y. W., & Lei, B. F. (2018). Supplemental blue light increases growth and quality of greenhouse pak choi... Journal of Integrative Agriculture, 17, 2245–2256. https://doi.org/10.1016/S2095-3119(18)62064-7

Author Biographies

Kaylee Ann Siswanto, SMP Cita Hati Christian School, Surabaya, Indonesia

Author Origin : Indonesia

Artadana Made Ida Bagus, Universitas Surabaya

Author Origin : Indonesia

Downloads

Download data is not yet available.

How to Cite

Siswanto, K. A., & Bagus, A. M. I. (2026). Blue Light Exposure Duration Improves Antioxidant Capacity in Mung Bean Seedlings (Vigna radiata L.). Jurnal Biologi Tropis, 26(2), 648–654. https://doi.org/10.29303/jbt.v26i2.11604

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.