Influence of Light Wavelength on the Growth Performance and Protein Content of Arthrospira platensis
DOI:
10.29303/jbt.v26i2.11925Published:
2026-04-28Downloads
Abstract
Microalgae play an important role in aquatic ecosystems as primary producers and have significant potential as a sustainable source of protein. One of the microalgae with high economic value is Arthrospira platensis, which is rich in bioactive compounds and protein content. Light is a key factor influencing microalgae growth, particularly its wavelength and spectrum, which can affect biomass production and biochemical composition. This study aimed to analyze the effect of different LED light wavelengths (red, blue, and a combination of red–blue) on the growth performance and protein content of Arthrospira platensis. The experiment was conducted in January 2026 using a completely randomized design with three treatments and three replications. The microalgae were cultivated in Walne medium under controlled conditions with continuous aeration for 14 days. Growth was monitored daily, and protein content was analyzed using a UV-Vis spectrophotometer at wavelengths of 280 nm and 260 nm. The results indicated that different light wavelengths significantly influenced the growth and protein content of Arthrospira platensis. The combination of red–blue light showed better performance compared to single wavelengths in enhancing biomass production and protein content. In conclusion, the use of combined LED light spectra can optimize the cultivation of Arthrospira platensis and improve its potential as an alternative protein source
Keywords:
Arthrospira platensis Growth performance Light spectrum Protein contentReferences
Amrulloh, M., & Dewi, R. (2026). Respon Pertumbuhan dan Pigmen Fotosintetik Klorofil-a Spirulina platensis pada Intensitas Cahaya Berbeda. Buletin Oseanografi Marina, 15(1), 168–180. https://doi.org/10.14710/buloma.v15i1.73449
Bryant, D. A., & Gisriel, C. J. (2024). The structural basis for light harvesting in organisms producing phycobiliproteins. The Plant Cell, 36(10), 4036–4064. https://doi.org/10.1093/plcell/koae126
Chini Zittelli, G., Mugnai, G., Milia, M., Cicchi, B., Silva Benavides, A. M., Angioni, A., Addis, P., & Torzillo, G. (2022). Effects of blue, orange and white lights on growth, chlorophyll fluorescence, and phycocyanin production of Arthrospira platensis cultures. Algal Research, 61, 102583. https://doi.org/10.1016/j.algal.2021.102583
Costa, B. R., Rocha, S. F., Rodrigues, M. C. K., Pohndorf, R. S., Larrosa, A. P. Q., & Pinto, L. A. A. (2015). Physicochemical characteristics of the Spirulina sp. Dried in heat pump and conventional tray dryers. International Journal of Food Science & Technology, 50(12), 2614–2620. https://doi.org/10.1111/ijfs.12930
De Rijdt, M., Verhaegen, J., Dohogne, D., Brostaux, Y., Haubruge, E., & Goffin, D. (2025). Impact of spirulina biomass physical form on texture, cooking properties, and sensory profile of enriched pasta and meat analogues. Applied Food Research, 5(2), 101328. https://doi.org/10.1016/j.afres.2025.101328
Georgieva, Z., Karcheva, Z., Toshkova-Yotova, T., Georgieva, A., Toshkova, R., Petrova, D., Zhiponova, M., & Chaneva, G. (2024). Blue Light Enhances the Antioxidant, Antimicrobial, and Antitumor Potential of the Green Microalgae Coelastrella sp. BGV. Plants, 13(23), 3295. https://doi.org/10.3390/plants13233295
Hasim, H., Akram, M., & Koniyo, Y. (2022). Kinerja Kepadatan Spirulina Sp. Yang diberi Salinitas Berbeda pada Media Kultur Walne. Jurnal Sumberdaya Akuatik Indopasifik, 6(2), 141–152. https://doi.org/10.46252/jsai-fpik-unipa.2022.Vol.6.No.2.234
Ilhamdy, A. F., Jumsurizal, & Darwin. (2020). Kultivasi Spirulina Plantesis menggunakan media Walne dalam Skala Laboratorium. Marinade, 3(02), 114–120. https://doi.org/10.31629/marinade.v3i02.2731
Iswati, I., Natsir, M. H., Ciptadi, G., & Susilawati, T. (2021). Pengaruh NaCl Fisiologis dan Ringer Laktat terhadap Kualitas Spermatozoa pada Suhu Ruang dan Fertilitas Telur Ayam Buras. Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science), 23(1), 33–42. https://doi.org/10.25077/jpi.23.1.33-42.2021
Jung, C. H. G., Waldeck, P., Sykora, S., Braune, S., Petrick, I., Küpper, J.-H., & Jung, F. (2022). Influence of Different Light-Emitting Diode Colors on Growth and Phycobiliprotein Generation of Arthrospira platensis. Life, 12(6), 895. https://doi.org/10.3390/life12060895
Leca, M.-A., Regnault, L., Sambusiti, C., Monlau, F., Le Guer, Y., & Beigbeder, J.-B. (2025). Pilot-scale cultivation of Arthrospira platensis using pretreated anaerobic digestate and geothermal water: A comparative study. Journal of Environmental Management, 393, 126984. https://doi.org/10.1016/j.jenvman.2025.126984
Lestari, M. D., Sajidan, & Pangastuti, A. (2025). Laju Pertumbuhan, Biomassa, dan Produksi Fikosianin Arthrospira platensis Perairan Pulau Jawa Pada Variasi Salinitas. Bioscientist : Jurnal Ilmiah Biologi, 13(4), 2848–2857. https://doi.org/10.33394/bioscientist.v13i4.18387
Li, B., Shi, X., Yang, S., Yang, L., Wang, X., Fan, C., Xie, Q., Jiang, Y., & Jiang, S. (2024). Impact of storage temperature on physical stability and protein-lipid Co-oxidation in whey protein functional emulsions. Food Bioscience, 62, 105433. https://doi.org/10.1016/j.fbio.2024.105433
Li, K., Ye, Q., Li, Q., Xia, R., Guo, W., & Cheng, J. (2020). Effects of the spatial and spectral distribution of red and blue light on Haematococcus pluvialis growth. Algal Research, 51, 102045. https://doi.org/10.1016/j.algal.2020.102045
Nghinaunye, T., Waldeck, P., Jung, C. G. H., Küpper, J.-H., Jung, F., & Braune, S. (2024). Response of Arthrospira platensis to different temperatures regarding growth and biochemical composition. Clinical Hemorheology and Microcirculation, 86(1–2), 205–211. https://doi.org/10.3233/CH-238104
Sampaio, I. C. F., Maestrello, C. C., de Moura, I. V. L., Estevam, R., de Morais, E. G., Ferrer, I., de Oliveira, J. P., & Cassini, S. T. A. (2024). Microalgae cell wall hydrolysis using snailase and mechanical sand milling. Algal Research, 78, 103425. https://doi.org/10.1016/j.algal.2024.103425
Sathinathan, P., Parab, H. M., Yusoff, R., & Ngoh, G. C. (2025). Optimizing microalgae growth under different illumination spectra preceding tubular photobioreactor fabrication. Biomass and Bioenergy, 201, 108088. https://doi.org/10.1016/j.biombioe.2025.108088
Spínola, M. P., Mendes, A. R., & Prates, J. A. M. (2024). Chemical Composition, Bioactivities, and Applications of Spirulina (Limnospira platensis) in Food, Feed, and Medicine. Foods, 13(22), 3656. https://doi.org/10.3390/foods13223656
Thoré, E. S. J., Muylaert, K., Bertram, M. G., & Brodin, T. (2023). Microalgae. Current Biology, 33(3), R91–R95. https://doi.org/10.1016/j.cub.2022.12.032
Trevi, S., Uren Webster, T., Consuegra, S., & Garcia de Leaniz, C. (2023). Benefits of the microalgae Spirulina and Schizochytrium in fish nutrition: A meta-analysis. Scientific Reports, 13(1), 2208. https://doi.org/10.1038/s41598-023-29183-x
Xu, Y., & Harvey, P. J. (2019). Carotenoid Production by Dunaliella salina under Red Light. Antioxidants, 8(5), 123. https://doi.org/10.3390/antiox8050123
Zhu, L., Kong, Y., Chang, X., Feng, J., Wang, X., Hou, L., Zhao, X., Pei, C., & Kong, X. (2023). Effects of two fish-derived probiotics on growth performance, innate immune response, intestinal health, and disease resistance of Procambarus clarkii. Aquaculture, 562, 738765. https://doi.org/10.1016/j.aquaculture.2022.738765
License
Copyright (c) 2026 Gabriela Sabatin Siama, Marvel Reuben Suwitono, Doli Situmeang

This work is licensed under a Creative Commons Attribution 4.0 International License.

Jurnal Biologi Tropis is licensed under a Creative Commons Attribution 4.0 International License.
The copyright of the received article shall be assigned to the author as the owner of the paper. The intended copyright includes the right to publish the article in various forms (including reprints). The journal maintains the publishing rights to the published articles.
Authors are permitted to disseminate published articles by sharing the link/DOI of the article at the journal. Authors are allowed to use their articles for any legal purposes deemed necessary without written permission from the journal with an acknowledgment of initial publication to this journal.























