Photoperiod Extension Enhances Lettuce Performance Across LED Spectra in an Indoor Vertical Farming Setup
DOI:
10.29303/jbt.v26i2.11752Published:
2026-05-02Downloads
Abstract
Artificial lighting is a key factor in indoor vertical farming because light spectrum and photoperiod strongly regulate plant growth, biomass accumulation, and pigment formation. Lettuce is highly responsive to light manipulation, making it an important model crop for evaluating lighting strategies that can improve productivity and quality under controlled environments. This study evaluated the effects of LED light color and photoperiod on the growth, yield, and pigment content of lettuce (Lactuca sativa L.) cultivated in an indoor vertical farming system in Yogyakarta, Indonesia (November 2025 – January 2026). A 3 × 3 factorial experiment arranged in a nested design was conducted with three light colors (white, blue, and green) and three photoperiods (8, 12, and 16 h). Data were analyzed using ANOVA (α = 0.05), followed by Tukey's HSD when significant differences were detected. Growth and yield were assessed using stem diameter, plant height, number of leaves, leaf area, root length, fresh weight, and dry weight, while physiological responses were evaluated using chlorophyll a, chlorophyll b, and total chlorophyll. Significant light color × photoperiod interactions were observed for stem diameter, root length, fresh and dry weight, and chlorophyll contents. Overall, white light produced superior lettuce performance compared with blue and green light, particularly under a 16-h photoperiod. The combination of white light and a 16-h photoperiod resulted in the highest biomass accumulation and pigment content, indicating that optimizing both light spectrum and photoperiod is critical to improve lettuce productivity and quality in indoor vertical farming.
Keywords:
Biomass yield Indoor vertical farming Light spectrum Lettuce PhotoperiodReferences
Alrajhi, A. A., Alsahli, A. S., Alhelal, I. M., Rihan, H. Z., Fuller, M. P., Alsadon, A. A., & Ibrahim, A. A. (2023). The Effect of LED Light Spectra on the Growth, Yield and Nutritional Value of Red and Green Lettuce (Lactuca sativa). Plants, 12(3), 463. https://doi.org/10.3390/plants12030463
Avianto, Y. (2025). The Impact of Calcium Supplementation on Physiological Activity and Cherelle Wilt Reduction in Cocoa (Theobroma cacao L.). Jurnal Biologi Tropis, 25(1), 179–189. https://doi.org/10.29303/jbt.v25i1.8292
Avianto, Y., Noviyanto, A., Jaya, G. I., Handru, A., Ferhat, A., Hartanto, E. S., Sidiq, M. F., Saputra, B. F., Ramadhani, J. N., & Shofry, M. A. (2024). Integrating Automated Drip Irrigation and Organic Matter to Improve Enzymatic Performance and Yield of Water Efficient Chilli in Karst Region. Journal of Ecological Engineering, 25(11), 175–187. https://doi.org/10.12911/22998993/192820
Avianto, Y., Riyadi, F., Hasibuan, S. B., & Nababan, J. G. (2025). Unraveling the Physiological Mechanisms and Bioaccumulation Efficacy of Lemna perpusilla for Heavy Metal Phytoremediation in Yogyakarta River Wastewater. Jurnal Biologi Tropis, 25(4b), 73–84. https://doi.org/10.29303/jbt.v25i4b.10387
Avianto, Y., & Saputra, B. F. (2024). Perbandingan Ekofisiologis Pucuk Teh pada Ketinggian Rendah dan Menengah di DIY. Jurnal Agrosains Dan Teknologi, 9(2), 59–69. https://doi.org/10.24853/jat.9.2.%25p
Avianto, Y., & Susila, W. A. (2024). Application techniques of photosynthesis bacteria and its effect on the growth and yield of local Bantul shallot variety (Allium cepa var. Aggregatum cv. Crok Kuning). JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 6(2). https://doi.org/10.36378/juatika.v6i2.3577
Boros, I. F., Székely, G., Balázs, L., Csambalik, L., & Sipos, L. (2023). Effects of LED lighting environments on lettuce (Lactuca sativa L.) in PFAL systems – A review. Scientia Horticulturae, 321, 112351. https://doi.org/10.1016/j.scienta.2023.112351
Carotti, L., Potente, G., Pennisi, G., Ruiz, K. B., Biondi, S., Crepaldi, A., Orsini, F., Gianquinto, G., & Antognoni, F. (2021). Pulsed LED Light: Exploring the Balance between Energy Use and Nutraceutical Properties in Indoor-Grown Lettuce. Agronomy, 11(6), 1106. https://doi.org/10.3390/agronomy11061106
Cho, J. Y., Yoo, K. S., Kim, J., Choi, B. J., & Oh, W. (2020). Growth and Bioactive Compounds of Lettuce as Affected by Light Intensity and Photoperiod in a Plant Factory Using External Electrode Fluorescent Lamps. Horticultural Science and Technology, 38(5), 645–659. https://doi.org/10.7235/HORT.20200059
Choi, C. S., Siregar, I. Z., & Ravi, S. (2020). Reframing the Competition for Land between Food and Energy Production in Indonesia. In S. J. Walsh, D. Riveros-Iregui, J. Arce-Nazario, & P. H. Page (Eds.), Land Cover and Land Use Change on Islands (pp. 241–260). Springer International Publishing. https://doi.org/10.1007/978-3-030-43973-6_11
Fei, S., Wu, R., Liu, H., Yang, F., & Wang, N. (2025). Technological Innovations in Urban and Peri-Urban Agriculture: Pathways to Sustainable Food Systems in Metropolises. Horticulturae, 11(2), 212. https://doi.org/10.3390/horticulturae11020212
Gao, Q., Liao, Q., Li, Q., Yang, Q., Wang, F., & Li, J. (2022). Effects of LED Red and Blue Light Component on Growth and Photosynthetic Characteristics of Coriander in Plant Factory. Horticulturae, 8(12), 1165. https://doi.org/10.3390/horticulturae8121165
García-Caparrós, P., Martínez-Ramírez, G., Almansa, E. M., Javier Barbero, F., Chica, R. M., & Teresa Lao, M. (2020). Growth, Photosynthesis, and Physiological Responses of Ornamental Plants to Complementation with Monochromic or Mixed Red-Blue LEDs for Use in Indoor Environments. Agronomy, 10(2), 284. https://doi.org/10.3390/agronomy10020284
Ghorbani, M., Azarnejad, N., Carril, P., Celletti, S., & Loppi, S. (2024). Boosting the resilience to drought of crop plants using wood distillate: A pilot study with lettuce (Lactuca sativa L.). Plant Stress, 12, 100450. https://doi.org/10.1016/j.stress.2024.100450
Hadikusuma, M. I., Sufandi, M. R., & Bakar, A. (2024). Otomasi Dan Pemantauan Budidaya Tanaman Hidroponik Dengan Teknik NFT Pada Indoor Vertical Farming Berbasis ESP32. Jurnal ELIT, 5(1), 82–91. https://doi.org/10.31573/elit.v5i1.726
Jasenovska, L., Brestic, M., Barboricova, M., Ferencova, J., Filacek, A., & Zivcak, M. (2024). Analysis of the effects of various light spectra on microgreen species. Folia Horticulturae, 36(2), 197–209. https://doi.org/10.2478/fhort-2023-0012
Li, J., Zhang, Y., Cheng, R., & Li, T. (2025). Light Spectrum, Intensity, and Photoperiod Are Key for Production as Well as Speed Breeding of Spring Wheat in Indoor Farming. Plant-Environment Interactions, 6(5), e70085. https://doi.org/10.1002/pei3.70085
Liu, J., & Van Iersel, M. W. (2021). Photosynthetic Physiology of Blue, Green, and Red Light: Light Intensity Effects and Underlying Mechanisms. Frontiers in Plant Science, 12, 619987. https://doi.org/10.3389/fpls.2021.619987
Matsuda, R., Ozawa, N., & Fujiwara, K. (2014). Leaf photosynthesis, plant growth, and carbohydrate accumulation of tomato under different photoperiods and diurnal temperature differences. Scientia Horticulturae, 170, 150–158. https://doi.org/10.1016/j.scienta.2014.03.014
Medina-Lozano, I., Bertolín, J. R., & Díaz, A. (2021). Nutritional value of commercial and traditional lettuce (Lactuca sativa L.) and wild relatives: Vitamin C and anthocyanin content. Food Chemistry, 359, 129864. https://doi.org/10.1016/j.foodchem.2021.129864
Mohammed, N., El-Hendawy, S., Alsamin, B., Mubushar, M., & Dewir, Y. H. (2023). Integrating Application Methods and Concentrations of Salicylic Acid as an Avenue to Enhance Growth, Production, and Water Use Efficiency of Wheat under Full and Deficit Irrigation in Arid Countries. Plants, 12(5), 1019. https://doi.org/10.3390/plants12051019
Nguyen, T. K. L., Cho, K. M., Lee, H. Y., Cho, D. Y., Lee, G. O., Jang, S. N., Lee, Y., Kim, D., & Son, K.-H. (2021). Effects of White LED Lighting with Specific Shorter Blue and/or Green Wavelength on the Growth and Quality of Two Lettuce Cultivars in a Vertical Farming System. Agronomy, 11(11), 2111. https://doi.org/10.3390/agronomy11112111
Novriani. (2014). Respon tanaman selada (Lactuca sativa L.) terhadap pemberian pupuk organik cair asal sampah organik pasar. Klorofil: Jurnal Ilmu-Ilmu Agroteknologi, 9(2), 57–61. https://doi.org/10.32502/jk.v9i2.112
Nurdianna, D., Putri, R. B. A., & Harjoko, D. (2018). Penggunaan Beberapa Komposisi Spektrum Led Pada Potensi Dan Hasil Hidroponik Indoor Selada Keriting Hijau. Agrosains: Jurnal Penelitian Agronomi, 20(1), 1. https://doi.org/10.20961/agsjpa.v20i1.26310
Pashkovskiy, P., Khalilova, L., Vereshchagin, M., Voronkov, A., Ivanova, T., Kosobryukhov, A. A., Allakhverdiev, S. I., Kreslavski, V. D., & Kuznetsov, V. V. (2023). Impact of varying light spectral compositions on photosynthesis, morphology, chloroplast ultrastructure, and expression of light-responsive genes in Marchantia polymorpha. Plant Physiology and Biochemistry, 203, 108044. https://doi.org/10.1016/j.plaphy.2023.108044
Pennisi, G., Orsini, F., Blasioli, S., Cellini, A., Crepaldi, A., Braschi, I., Spinelli, F., Nicola, S., Fernandez, J. A., Stanghellini, C., Gianquinto, G., & Marcelis, L. F. M. (2019). Resource use efficiency of indoor lettuce (Lactuca sativa L.) cultivation as affected by red:blue ratio provided by LED lighting. Scientific Reports, 9(1), 14127. https://doi.org/10.1038/s41598-019-50783-z
Sengodan, P. (2022). An Overview of Vertical Farming: Highlighting the Potential in Malaysian High-Rise Buildings. Pertanika Journal of Science and Technology, 30(2), 949–981. https://doi.org/10.47836/pjst.30.2.06
Smith, H. L., McAusland, L., & Murchie, E. H. (2017). Don’t ignore the green light: Exploring diverse roles in plant processes. Journal of Experimental Botany, 68(9), 2099–2110. https://doi.org/10.1093/jxb/erx098
Suhandoko, A. A., Sumarsono, S., & Purbajanti, E. D. (2018). Produksi selada (Lactuca sativa l.) dengan penyinaran lampu led merah dan biru di malam hari pada teknologi hidroponik sistem terapung termodifikasi. Journal of Agro Complex, 2(1), 79. https://doi.org/10.14710/joac.2.1.79-85
Supriani, E., Budiyanto, S., & Sutarno, S. (2021). Respon Tanaman Selada Keriting Hijau Terhadap Penyinaran Lampu LED dan Konsentrasi CaCl2 pada Sistem Hidroponik. AGROVITAL : Jurnal Ilmu Pertanian, 6(2), 99. https://doi.org/10.35329/agrovital.v6i2.2713
Thomas, T., Biradar, M. S., Chimmad, V. P., & Janagoudar, B. S. (2021). Growth and physiology of lettuce (Lactuca sativa L.) cultivars under different growing systems. Plant Physiology Reports, 26(3), 526–534. https://doi.org/10.1007/s40502-021-00591-3
Tran, T. P., Tran, D. V., Phan, V. K., Nguyen, D. T., Cao, T. S., Nguyen, D. L., & Vo, H. C. (2025). Factors influencing agricultural land transformation for climate change adaptation in Can Loc district, Ha Tinh province, Vietnam. Environmental and Sustainability Indicators, 25, 100576. https://doi.org/10.1016/j.indic.2024.100576
Widodo, S., Solahudin, M., & Sucahyo, L. (2022). Pertumbuhan dan Efisiensi Penggunaan Energi pada Budidaya Selada dalam Plant factory dengan Beberapa Perlakuan Fotoperiode. Jurnal Keteknikan Pertanian Tropis Dan Biosistem, 10(2), 154–161. https://doi.org/10.21776/ub.jkptb.2022.010.02.08
Yudina, L., Sukhova, E., Gromova, E., Mudrilov, M., Zolin, Y., Popova, A., Nerush, V., Pecherina, A., Grishin, A. A., Dorokhov, A. A., & Sukhov, V. (2023). Effect of Duration of LED Lighting on Growth, Photosynthesis and Respiration in Lettuce. Plants, 12(3), 442. https://doi.org/10.3390/plants12030442
Zhang, X., He, D., Niu, G., Yan, Z., & Song, J. (2018). Effects of environment lighting on the growth, photosynthesis, and quality of hydroponic lettuce in a plant factory. Int J Agric & Biol Eng, 11(2), 33–40. https://doi.org/10.25165/j.ijabe.20181102.3420
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