Effect of Formaldehyde Inhalation on Leukocyte Counts and Malondialdehyde Levels in Wistar Rats (Rattus norvegicus)

Muhammad  Fauzan; Ima Arum Lestarini; Anak Agung Ayu Niti  Wedayani

doi:10.29303/jbt.v23i1.6087

Effect of Formaldehyde Inhalation on Leukocyte Counts and Malondialdehyde Levels in Wistar Rats (Rattus norvegicus)

Authors

Muhammad Fauzan , Ima Arum Lestarini , Anak Agung Ayu Niti Wedayani

DOI:

10.29303/jbt.v23i1.6087

Published:

2023-12-04

Issue:

Vol. 23 No. 1 (2023): Special Issue

Keywords:

Formaldehyde, formalin, leukocyte, malondialdehyde, wistar rats.

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Fauzan, M. ., Lestarini, I. A., & Wedayani, A. A. A. N. . (2023). Effect of Formaldehyde Inhalation on Leukocyte Counts and Malondialdehyde Levels in Wistar Rats (Rattus norvegicus). Jurnal Biologi Tropis, 23(1), 348–352. https://doi.org/10.29303/jbt.v23i1.6087

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Abstract

Formaldehyde is a chemical compound that is often found in everyday life, such as in the chemical industry to research laboratories. Formaldehyde has toxic properties that can harm humans in long term exposure. The genotoxic and cytotoxic properties of formaldehyde can cause oxidative stress. This study assesses the number of leukocytes and levels of malondialdehyde in Wistar rats induced by 40 ppm formaldehyde. This study used 12 Wistar rats taken at random and put into 2 groups, namely the control and treatment groups. The treatment group was given formaldehyde inhalation at a concentration of 40 ppm for 16 weeks. At the 16th week, rat blood was taken retroorbital to calculate the number of leukocytes and levels of malondialdehyde. The calculation results were then tested statistically using IBM SPSS Statistics 25 software. The results showed that there was no significant difference (p>0.05) between the number of leukocytes in the control group and the treatment group. This study also showed that there was a significant difference (p<0.05) between malondialdehyde levels in the control group and the treatment group. In conclusion, there was no effect of formaldehyde induction with a concentration of 40 ppm on the number of leukocytes and there was an effect of formaldehyde induction with a concentration of 40 ppm on malondialdehyde levels in Wistar rats.

References

Al-Sarraj, A., & Al-Habity, A. (2013). Effect of formaldehyde vapor on the blood constituents of male rabbits. Iraqi Journal of Veterinary Sciences, 27(1), 15–19. DOI: https://doi.org/10.33899/ijvs.2013.82864

Asare-Donkor, N. K., Kusi Appiah, J., Torve, V., Voegborlo, R. B., & Adimado, A. A. (2020). Formaldehyde Exposure and Its Potential Health Risk in Some Beauty Salons in Kumasi Metropolis. Journal of Toxicology, 2020, 8875167. DOI: https://doi.org/10.1155/2020/8875167

Ayala, A., Muñoz, M. F., & Argüelles, S. (2014). Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxidative Medicine and Cellular Longevity, 2014, 360438. DOI: https://doi.org/10.1155/2014/360438

Ghelli, F., Bellisario, V., Squillacioti, G., Panizzolo, M., Santovito, A., & Bono, R. (2021). Formaldehyde in Hospitals Induces Oxidative Stress: The Role of GSTT1 and GSTM1 Polymorphisms. Toxics, 9(8). DOI: https://doi.org/10.3390/toxics9080178

Kang, D. S., Kim, H. S., Jung, J. H., Lee, C. M., Ahn, Y. S., & Seo, Y. R. (2021). Formaldehyde exposure and leukemia risk: a comprehensive review and network-based toxicogenomic approach. Genes and Environment, 43(1), 1–10. DOI: https://doi.org/10.1371/journal.pone.0179231

Mittal, M., Siddiqui, M. R., Tran, K., Reddy, S. P., & Malik, A. B. (2014). Reactive oxygen species in inflammation and tissue injury. Antioxidants and Redox Signaling, 20(7), 1126–1167. DOI: https://doi.org/10.1089/ars.2012.5149

Mohammed, N., Ahmed, S. A., Hegazy, N. I., & Kashishy, K. (2021). Ameliorative effects of hesperidin and N-acetylcysteine against formaldehyde-induced-hemato- And genotoxicity. Toxicology Research, 10(5), 992–1002. DOI: https://doi.org/10.1093/toxres/tfab083

Protano, C., Buomprisco, G., Cammalleri, V., Pocino, R. N., Marotta, D., Simonazzi, S., Cardoni, F., Petyx, M., Iavicoli, S., & Vitali, M. (2022). The carcinogenic effects of formaldehyde occupational exposure: A systematic review. Cancers, 14(1), 1–12. https://doi.org/10.3390/cancers14010165

Pullen Fedinick, K., Yiliqi, I., Lam, Y., Lennett, D., Singla, V., Rotkin-Ellman, M., & Sass, J. (2021). A cumulative framework for identifying overburdened populations under the toxic substances control act: Formaldehyde case study. International Journal of Environmental Research and Public Health, 18(11). DOI: https://doi.org/10.3390/ijerph18116002

Ranneh, Y., Ali, F., Akim, A. M., Hamid, H. A., Khazaai, H., & Fadel, A. (2017). Crosstalk between reactive oxygen species and pro-inflammatory markers in developing various chronic diseases: a review. Applied Biological Chemistry, 60(3), 327–338. DOI: https://doi.org/10.1007/s13765-017-0285-9

Sahlol, A. T., Kollmannsberger, P., & Ewees, A. A. (2020). Efficient Classification of White Blood Cell Leukemia with Improved Swarm Optimization of Deep Features. Scientific Reports, 10(1), 1–11. DOI: https://doi.org/10.1038/s41598-020-59215-9

Susilawati, N. K., Yasa, I. W. P. S., Suardana, W., Maliawan, S., Jawi, I. M., Romdhoni, A. C., Kadriyan, H., Putra, I. W. G. A. E., & Arijana, I. G. K. N. (2022). Purple Sweet Potato Reduces Malondialdehyde and TNF-a, Increases p53, and Protects Histopathological Appearance in Formaldehyde-induced Nasopharyngeal Carcinoma Rats. Indonesian Biomedical Journal, 14(2), 211–217. DOI: DOI: https://doi.org/10.18585/inabj.v14i2.1906

Tesfaye, S., Hamba, N., Gerbi, A., & Negeri, Z. (2021). Occupational formaldehyde exposure linked to increased systemic health impairments and counteracting beneficial effects of selected antioxidants. Alexandria Journal of Medicine, 57(1), 157–167. DOI: https://doi.org/10.1080/20905068.2021.1926172

Uluçam, E., & Bakar, E. (2016). The effect of proanthocyanidin on formaldehyde-induced toxicity in rat testes. Turkish Journal of Medical Sciences, 46(1), 185–193. DOI: https://doi.org/10.3906/sag-1411-13

Wultsch, G., Nersesyan, A., Kundi, M., Wagner, K. H., Ferk, F., Jakse, R., & Knasmueller, S. (2015). Impact of exposure to wood dust on genotoxicity and cytotoxicity in exfoliated buccal and nasal cells. Mutagenesis, 30(5), 701–709. DOI: https://doi.org/10.1093/mutage/gev034

Zhang, L., Tang, X., Rothman, N., Vermeulen, R., Ji, Z., Shen, M., Qiu, C., Guo, W., Liu, S., Reiss, B., Freeman, L. B., Ge, Y., Hubbard, A. E., Hua, M., Blair, A., Galvan, N., Ruan, X., Alter, B. P., Xin, K. X., … Lan, Q. (2010). Occupational exposure to formaldehyde, hematotoxicity, and leukemia-specific chromosome changes in cultured myeloid progenitor cells. Cancer Epidemiology, Biomarkers & Prevention : A Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology, 19(1), 80–88. DOI: https://doi.org/10.1158/1055-9965.EPI-09-0762

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Effect of Formaldehyde Inhalation on Leukocyte Counts and Malondialdehyde Levels in Wistar Rats (Rattus norvegicus)

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